Cutting-edge: bionanomaterial solutions in the battle against Severe Acute Respiratory Syndrome Coronavirus 2 by U. Saeed, R. Uppal, M. H. Najmi, I. Fazal, A. A. Khan, Z. Z. Piracha, M. R. Uppal, H. N. Ijaz, D. U. Ozsahin, B. Uzun, I. Ozsahin

“…Their role in automating nucleic acid extraction and purification has proven indispensable, particularly in the diagnostic milieu of SARS-CoV-2. …”
Get full text

Characterizations of cytokines and viral genomes in serum of patients with Dabie bandavirus infection by Zefeng Dong, Man Yuan, Yueping Xing, Hongkai Zhang, Qiang Shen

“…We detected and analyzed the cytokine concentrations and viral genomes in the serum samples of these patients to identify the possible causes of the patients’ deaths and to analyze the viral genetic characteristics.MethodsBlood serum specimens were obtained from the 10 individuals with Dabie bandavirus infection in Suzhou City. The specific nucleic acid of Dabie bandavirus was detected using a real-time reverse transcription polymerase chain reaction assay (RT-qPCR). …”
Get full text

Utilizing integrated bioinformatics and machine learning approaches to elucidate biomarkers linking sepsis to purine metabolism-associated genes by Fanqi Liang, Man Zheng, Jingjiu Lu, Peng Liu, Xinyu Chen

“…Among these, purine metabolism plays a crucial role in nucleic acid synthesis. However, the contribution of Purine Metabolism Genes (PMGs) to the defense mechanisms against sepsis remains inadequately explored. …”
Get full text

Pathogenetic therapeutic approaches for endocrine diseases based on antisense oligonucleotides and RNA-interference by Olga Golounina, Ildar Minniakhmetov, Ramil Salakhov, Rita Khusainova, Ekaterina Zakharova, Igor Bychkov, Natalia Mokrysheva

“…Molecular therapy uses nucleic acid-based therapeutics agents and becomes a promising alternative for disease conditions unresponsive to traditional pharmaceutical approaches. …”
Get full text

The combined manifestations of dramatically sore throat, congested and edematous mucosa, no-swelling tonsil are specific in acute Omicron pharyngitis by Lei Zhou, Lineng Zhang, Feng Xu

“…When compared with the gold standard measurement of Sars-Cov-2 virus antigen or nucleic acid, the diagnostic panel has a sensitivity of 66.7% and a specificity of 91.7%. …”
Get full text

Research on the Mechanism of Mannose Oligosaccharide Enhancing the Inhibitory Effect of Bifidobacterium breve on the Growth of Fusobacterium nucleatum by Xuyan ZHANG, Ruijie LIU, Bo ZHAO, Xiaoxi XU

“…The apparent structure was obviously damaged, protein and nucleic acid flowed out, and the integrity and permeability of the cell membrane were destroyed. …”
Get full text

Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae) by Mercy Makopa, Benjamin Mangiza, Benjamin Banda, Winnie Mozirandi, Molly Mombeshora, Stanley Mukanganyama

“…Only the acetone extract effected nucleic acid leakage. Screening of extracts’ potential to inhibit the activity of α-glucosidase was carried out spectrophotometrically following the production of p-nitrophenol from p-nitrophenol-glucopyranoside (substrate) at a wavelength of 405 nm. …”
Get full text

Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…In this article, we discuss in detail basic principles of the method, the processes that influence the result of genotyping, criteria for selecting optimal primers and probes, and the use of locked nucleic acid modifications in oligonucleotides as well as provide a protocol for the selection of primers and probes and for PCR by means of rs11121704 as an example. …”
Get full text

Occult Hepatitis B Infection among Hemodialysis Patients in Tabriz, Northwest of Iran: Prevalence and Mutations within the S Region by Narges Eslami, Vahdat Poortahmasebi, Javid Sadeghi, Reza Ghotaslou, Bahram Niknafs, Hossein Bannazadeh Baghi, Mahin Ahangar Oskouee

“…HBV serologic screening approaches need to be renewed to cover nucleic acid testing in the setting of hemodialysis and all the other high-risk groups associated with it (i.e., blood and organ donors).…”
Get full text

Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China by Le Chang, Junpeng Zhao, Fei Guo, Huimin Ji, Lu Zhang, Xinyi Jiang, Lunan Wang

“…All plasma samples were tested by eight ELISA assays in 16 blood centers, followed by the detection of CLIA and ECLIA methods in the National Center for Clinical Laboratories (NCCL), further confirmed by nucleic acid testing (NAT) and Western blot (WB). Results. …”
Get full text

Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital by Funda Şahin, Nida Özcan, Erdal Özbek, Selahattin Atmaca, Hakan Temiz

“…The Biospeedy viral nucleic acid isolation kit (Bioeksen, Türkiye) was used for the isolation of genetic material. …”
Get full text

Molecular Epidemiology of SARS-CoV-2 within Accra Metropolis Postlockdown by Frank T. Aboagye, Lawrence Annison, Henry Kwadwo Hackman, Maame E. Acquah, Yvonne Ashong, Isaac Owusu-Frimpong, Bill C. Egyam, Sharon Annison, George Osei-Adjei, Samuel Antwi-Baffour

“…Samples were subjected to nucleic acid extraction and followed by real-time polymerase chain reaction for the detection and quantification of SARS-CoV-2 RNA. …”
Get full text

Prognostic importance of human leukocyte antigen DRβ1 gene and protein tyrosine phosphatase nonreceptor Type 22 gene polymorphism in rheumatoid arthritis by Pramod Kumar Verma, Usha Singh, Shyam Kumar Saraf, Narendra Kumar Singh

“…HLA DRβ1 typing was done by low-resolution sequence-specific primer polymerase chain reaction and auto-antibodies (Abs) (rheumatoid factor [RF], anti-cyclic citrullinated peptide 2 (CCP2) Ab, anti-nuclear Ab, double-stranded deoxyribose nucleic acid, anticardiolipin Ab) were done by enzyme-linked immunosorbent assay. …”
Get full text

Protein-DNA interactions: statistical analysis of interatomic contacts in major and minor grooves by A. A. Anashkina, E. N. Kuznetsov, A. V.  Batianovskii, L. A. Uroshlev, V. G. Tumanyan, N. G. Esipova

“…Analysis of the accessible surface area of atoms in the major and the minor grooves showed a correlation with the number of contacts (coefficient of linear correlation 0.94 and 0.93, respectively), however, nucleic acid atoms forming hydrogen bonds make contacts more often than may be expected from statistical considerations. …”
Get full text

Prevalence and Residual Risk of HIV in Volunteer Blood Donors of Zhejiang Province, China, from 2018 to 2022 by Hong Zhu, Wei Ding, Wenjuan Han, Xiaofan Zheng, Yiqing Hu, Jie Dong, Yaling Wu, Danxiao Wu, Jinhui Liu, Faming Zhu

“…Blood safety levels have been significantly improved since the implementation of nucleic acid amplification technology (NAT) testing for blood donors. …”
Get full text

Clinical performance of two commercially available rapid antigen tests for influenza, RSV, and SARS-CoV-2 diagnostics by Laura E. Savolainen, Joanna Peltola, Risto Hilla, Tapio Åman, Markku Broas, Ilkka S. Junttila

“…ABSTRACT Rapid antigen tests (RATs) might provide rapid, low-cost, and easy-to-perform choice to nucleic-acid amplification methods (NAAT) in point-of-care (POC) diagnostics of respiratory infections. …”
Get full text

Altered O-Glycans in stimulated whole saliva from patients with primary Sjögren’s syndrome and non-pSS sicca by Sarah Kamounah, Kristina A. Thomsson, Christiane Elisabeth Sørensen, Eric Paul Bennett, Niclas G. Karlsson, Anne Marie Lynge Pedersen

Get full text

The Escalating Magnitude of COVID-19 Infections among the Northeastern Ethiopia Region: A Community-Based Cross-Sectional Study by Zeleke Geto, Saba Gebremichael, Melaku Ashagrie Belete, Alemu Gedefie, Genet Molla, Melkam Tesfaye, Wondmagegn Demsiss, Daniel Gebretsadik

“…After extraction of viral nucleic acid from oropharyngeal specimen, the real-time fluorescent polymerase chain reaction (RT-PCR) kit was used for detecting novel coronavirus. …”
Get full text

A Spike-Based mRNA Vaccine Encapsulated in Phospholipid 1,2-Dioleoyl-sn-Glycero-3-PhosphoEthanolamine Containing Lipid Nanoparticles Induced Potent B- and T-Cell Responses Associat... by Afshana Quadiri, Swayam Prakash, Latifa Zayou, Nisha Rajeswari Dhanushkodi, Amruth Chilukuri, Gemma Ryan, Kelly Wang, Hawa Vahed, Aziz A. Chentoufi, Lbachir BenMohamed

“…Studies on using the mRNA/LNP platform for vaccines have largely focused on the nucleic acid cargo with less attention paid to the LNP vehicle. …”
Get full text

Analytic and clinical validity of thyroid nodule mutational profiling using droplet digital polymerase chain reaction by Vincent L. Biron, Ashlee Matkin, Morris Kostiuk, Jordana Williams, David W. Cote, Jeffrey Harris, Hadi Seikaly, Daniel A. O’Connell

“…Droplet digital PCR (ddPCR) is an ultrasensitive method of nucleic acid detection that is particularly useful for identifying gene mutations. …”
Get full text