Effectiveness of inactivated influenza vaccine against laboratory-confirmed influenza in elderly Chinese patients with diabetes: A test-negative design case-control study by Zhengyan Chen, Tianchi Yang

“…A total of 2,204 elderly patients were tested for influenza virus nucleic acid or antigen during the study period. After applying exclusion criteria, 199 influenza-positive cases and 199 matched influenza-negative controls were included in the final analysis. …”
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Gene Editing in Pluripotent Stem Cells and Their Derived Organoids by Hang Zhou, Yun Wang, Li-Ping Liu, Yu-Mei Li, Yun-Wen Zheng

“…Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research and development boom with significant implications in regenerative medicine. …”
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Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates by Amin Hadi, Abbas Rastgoo, Maryam Eskandarian, Nooshin Haghighipour, Azam Bolhassani

“…Cell viability and low nucleic acid expression are the problems related to dendritic cells (DCs) and mesenchymal stem cells (MSCs), which are transfected with plasmid DNA. …”
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Analysis of multidrug resistant group B streptococci with reduced penicillin susceptibility forming small, less hemolytic colonies. by Hirotsugu Banno, Kouji Kimura, Yosuke Tanaka, Tsuyoshi Sekizuka, Makoto Kuroda, Wanchun Jin, Jun-Ichi Wachino, Keiko Yamada, Keigo Shibayama, Yoshichika Arakawa

“…We identified a 276_277insG nucleic acid insertion in the thiamin pyrophosphokinase (tpk) gene, resulting in premature termination at amino acid 103 in TPK, as a candidate mutation responsible for small colony formation. …”
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Role of ixodid ticks in tick-borne pathogen spread and circulation in the Belarusian Lakeland by A. A. Osmolovsky, I. A. Subotsina

“….), Babesia spp. and Tick-borne encephalitis virus genetic materials with real-time polymerase chain reaction using the reagent kit for nucleic acid extraction from environmental samples in accordance with the manufacturer’s instruc­tions. …”
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The Role of Surveillance Inspections in Reducing False-Positives of SARS-CoV-2 Omicron Variants during the COVID-19 Epidemic by Li-Li Liu, Yu-Hong Zheng, You-Quan Zhang, Jin-Piao Lin, Zhi-Lin Luo, Liu-Min Yu, Shi-Hua Gao, Falin Chen

“…Daily surveillance inspection in laboratories can remind laboratories to strictly comply with standard procedures, focus on laboratory quality control, and reduce the occurrence of false-positive cases in SARS-CoV-2 nucleic acid tests to some extent. This study recommends that government decision-making departments establish policies and arrange experts to conduct daily surveillance inspections to improve laboratory quality control.…”
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Analysis of the Effect of Female Genital Tuberculosis on Ovarian Reserve Parameters by Sharma Richa, Kanhere Anjali, Jain Sonal, Jain Akrati

“…Materials and Methods: A total of 133 infertile women who underwent diagnostic hysterolaparoscopy and cartridge-based nucleic acid amplification test testing of an endometrial biopsy were included in the study. …”
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Mycoplasma pneumoniae detections in children with acute respiratory infection, 2010–2023: a large sample study in China by Yuzhu Miao, Jungen Li, Linlin Huang, Ting Shi, Tingbo Jiang

“…Methods Children with ARTIs admitted to the Children’s Hospital of Soochow University (SCH) from January 2010 to December 2023 and underwent MP nucleic acid PCR assay were included. Clinical data on age, sex, onset time and detection result were collected and analyzed. …”
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Impact of deformed wing virus master variants (dwv-a, dwv-b, and dwv-c) in managed honey bee colonies of türkiye by Mustafa Emin Oz, Oguzhan Avci, Muge Dogan

“…<p> <b>Materials and Methods:</b> For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively.…”
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Purification and Characterization of Hyaluronic Acid Produced by Priestia flexa N7 by Dinh Giap Vu, Quang Tung Nguyen, Thi Cam Van Do, Thi Cuong Vu, Nguyen Phuong Dai Nguyen

“…Methods: The biochemical methods used in this study included Superdex™ G100 columns chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, protein and nucleic acid quantitation assays, and purification of HA. …”
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The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al-Najaf, Iraq by Aoula Al-Zebeeby, Ali Hadi Abbas, Haider Abas Alsaegh, Furkan Sabbar Alaraji

“…The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. …”
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Study of some virulence genes from uropathogenic Klebsiella pneumoniae and Pseudomonas aeruginosa isolated in Wasit Province, Iraq by Rana H. Raheema, Sabaa Hilal Hadi, Zainab Adil Ghani Chabuck

“…When DNA was recovered from the isolates, the nucleic acid purity was diverse from 1.8 to 2.0, and the concentration fluctuated from 50 to 360 mg/µL. …”
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Molecular epidemiology of foot-and-mouth disease (review) by A. V. Scherbakov

“…These tests are based on constantly progressing technologies of nucleic acid sequencing and phylogenetic analysis. The use of these technologies made it possible to assess the genetic diversity of the FMD virus, to analyze the evolution of the virus in the enzootic regions, and track the pathways of FMD epizootic and panzootic spread. …”
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Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus by George Tetz, Kristina Kardava, Maria Vecherkovskaya, Alireza Khodadadi-Jamayran, Aristotelis Tsirigos, Victor Tetz

“…The results of this study add another line of evidence that the Universal Receptive System plays an important role in cell regulation, including cell responses to the environmental factors of clinically important pathogens, and that nucleic acid-based TezRs are functionally active parts of the extrabiome.…”
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Naked-Eye Molecular Testing for the Detection of <i>Xylella fastidiosa</i> in Mallorca (Balearic Island) Almond Orchards by Colorimetric LAMP by Amoia Serafina Serena, Ana Falcón-Piñeiro, Milica Pastar, José Manuel Garcìa-Madero, Nicoletta Contaldo, Mikael Muegge, Stéphane Compant, Pasquale Saldarelli, Angelantonio Minafra

“…Molecular detection through isothermal amplification reactions enables simplified instrumentation and the use of raw nucleic acid extracts. Colorimetric loop-mediated isothermal amplification (cLAMP) was applied to rapidly detect <i>Xf</i> in naturally infected almonds on Mallorca Island (Spain), using a quick crude sap extraction without DNA purification. …”
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Ticks and Tick-Borne Pathogens in Wild Animals and Birds from Two Rehabilitation Facilities in Greece by Dimitrios Vourvidis, Georgia Tzouganatou, Sokratis Perdikaris, Evangelia Kofidou, Beatriz Martinez-Gonzalez, Mary Emmanouil, Emmanouil Papadogiannakis, Anastasia Komnenou, Emmanouil Angelakis

“…., <i>Bartonella</i> spp., or viral nucleic acid was found. Phylogenetic analysis revealed close genetic relationships between the detected <i>Rickettsia</i> species and those previously reported in neighboring regions. …”
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Optimization of Fermentation Conditions for Increasing Erucamide Content in <i>Bacillus megaterium</i> Using Several Accelerants by Hao Zhao, Yudan Xie, Zhu Li, Longfeng Wei, Renli Ai

“…The mechanism of membrane metabolism in the L2 strain was further investigated, and our data suggested that the conductivity, nucleic acid and protein content, and β-galactosidase activity of L2 were increased significantly after treatment with accelerants, indicating that the accelerants changed the cell membrane permeability of the L2 strain but did not harm or kill the bacteria. …”
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Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice by Denis N. Kisakov, Larisa I. Karpenko, Lyubov A. Kisakova, Sergey V. Sharabrin, Mariya B. Borgoyakova, Ekaterina V. Starostina, Oleg S. Taranov, Elena K. Ivleva, Oleg V. Pyankov, Anna V. Zaykovskaya, Elena V. Dmitrienko, Vladimir A. Yakovlev, Elena V. Tigeeva, Irina Alekseevna Bauer, Svetlana I. Krasnikova, Nadezhda B. Rudometova, Andrey P. Rudometov, Artemiy A. Sergeev, Alexander A. Ilyichev

“…The development of naked mRNA vaccines could improve vaccine tolerability. Naked nucleic acid delivery using the jet injection method may be a solution. …”
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Enterococcus durans SL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn (Triticum monococcum L. ssp. monococcum) by Berna Genc, Seyda Merve Karatas, Merve Tuğçe Tunç

“…This anionic heteropolysaccharide had, in addition to the carbohydrate backbone, a protein structure that did not contain nucleic acid. The carbohydrate backbone consisted of mannose, glucose, rhamnose, arabinose, xylose and galactose. …”
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Rap1 and mTOR signaling pathways drive opposing immunotoxic effects of structurally similar aryl-OPFRs, TPHP and TOCP by Bilin Zhao, Shuang Zheng, Gaoxiang Yang, Zhijun He, Jiewei Deng, Lijuan Luo, Xinyan Li, Tiangang Luan

“…These results revealed comparable disturbances induced by both compounds, including disruptions in nucleic acid synthesis and energy metabolism, blocking ADP to ATP conversion by reducing TCA cycle intermediates, consequently leading to ATP depletion. …”
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