The Psu protein of phage satellite P4 inhibits transcription termination factor ρ by forced hyper-oligomerization by Daniela Gjorgjevikj, Naveen Kumar, Bing Wang, Tarek Hilal, Nelly Said, Bernhard Loll, Irina Artsimovitch, Ranjan Sen, Markus C. Wahl

“…ATPase, nucleotide binding and nucleic acid binding studies revealed that Psu hinders ρ ring closure and traps nucleotides in their binding pockets on ρ. …”
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Substrate transport and drug interaction of human thiamine transporters SLC19A2/A3 by Peipei Li, Zhini Zhu, Yong Wang, Xuyuan Zhang, Chuanhui Yang, Yalan Zhu, Zixuan Zhou, Yulin Chao, Yonghui Long, Yina Gao, Songqing Liu, Liguo Zhang, Pu Gao, Qianhui Qu

“…Abstract Thiamine and pyridoxine are essential B vitamins that serve as enzymatic cofactors in energy metabolism, protein and nucleic acid biosynthesis, and neurotransmitter production. …”
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CD133+PD-L1+ cancer cells confer resistance to adoptively transferred engineered macrophage-based therapy in melanoma by Jiaojiao Xu, Zhe Li, Qinli Tong, Sihang Zhang, Jianchen Fang, Aihua Wu, Guoguang Wei, Chen Zhang, Sheng Yu, Binbin Zheng, Hongzheng Lin, Xueling Liao, Zeyu Xiao, Wei Lu

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Classification, Mechanisms of Action, and Therapeutic Applications of Inhibitory Oligonucleotides for Toll-Like Receptors (TLR) 7 and 9 by Petar S. Lenert

“…Interestingly, B cells also express some innate sensors, like TLR7 and TLR9, and may respond to bacterial hypomethylated CpG motifs and single-stranded RNA viruses. Intracellular nucleic acid sensing TLRs play an important role in the pathogenesis of Systemic Lupus Erythematosus (SLE). …”
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INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform by Chun Ngo Yau, Jacky Tin Shing Hung, Robert A. A. Campbell, Thomas Chun Yip Wong, Bei Huang, Ben Tin Yan Wong, Nick King Ngai Chow, Lichun Zhang, Eldric Pui Lam Tsoi, Yuqi Tan, Joshua Jing Xi Li, Yun Kwok Wing, Hei Ming Lai

“…INSIHGT also allows multi-round, highly multiplexed 3D molecular probing and is compatible with downstream traditional histology and nucleic acid sequencing. With INSIHGT, we map undescribed podocyte-to-parietal epithelial cell microfilaments in mouse glomeruli and neurofilament-intensive inclusion bodies in the human cerebellum, and identify NPY-proximal cell types defined by spatial morpho-proteomics in mouse hypothalamus. …”
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Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies by Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Rita Lasickienė, Artiomas Akatov, Gabrielis Kundrotas, Vilimas Sereika, Raimundas Lelešius, Aurelija Žvirblienė, Kęstutis Sasnauskas

“…The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and inserted into yeast Saccharomyces cerevisiae expression plasmid. …”
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A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9 by Akram Hosen, Md Nakibul Hasan Nishat, MD. Mohaimin Hossain Soaib, Omar Sharif Sharkar, Md. Sahabuddin, Imdadul Haque Sharif, Sujay Kumar Bhajan

“…ABSTRACT CRISPR Cas system is a revolutionary and precise genome editing system that induces bacterial and archaeal adaptive immunity and already has shown several promising applications in the treatment of genetic diseases and detection of nucleic acid targets. It is a complex tool, comprising diverse effector proteins and guide RNA that can be applied to dissociate and modify genes in living organisms along with the in vitro process. …”
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Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens by Govindan Muthukumar, Ayyanar Kamalakannan, Irudhayasamy Johnson, Pachamuthu Kamaraj, Iyyamperumal Muthuvel, Shanmugam Varanavasiappan

“…Age-old management practices are time-consuming and labour-intensive processes. In the past, nucleic acid-based methods, such as hybridization, amplification, and sequencing, have been used extensively for the preliminary identification of plant pathogens. …”
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Structural basis of RNA polymerase complexes in African swine fever virus by Guoliang Zhu, Fei Xi, Wuxia Zeng, Yifei Zhao, Weijun Cao, Chen Liu, Fan Yang, Yi Ru, Shuqi Xiao, Shilei Zhang, Huanan Liu, Hong Tian, Fayu Yang, Biao Lu, Shukai Sun, Haiyang Song, Bozhang Sun, Xiaoyi Zhao, Lijie Tang, Kangli Li, Jijun He, Jianhong Guo, Yun Zhu, Zixiang Zhu, Fei Sun, Haixue Zheng

“…Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. …”
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Discordant Clostridioides difficile diagnostic assay and treatment practice: a cross-sectional study in a tertiary care hospital, Geneva, Switzerland by Antoine Poncet, Laurent Kaiser, Lauriane Lenggenhager, Marie-Céline Zanella, Jacques Schrenzel

“…Objectives To determine the proportion of patients who received a treatment for Clostridioides difficile infection (CDI) among those presenting a discordant C. difficile diagnostic assay and to identify patient characteristics associated with the decision to treat CDI.Design Cross-sectional study.Setting Monocentric study in a tertiary care hospital, Geneva, Switzerland.Participants Among 4562 adult patients tested for C. difficile between March 2017 and March 2019, 208 patients with discordant tests’ results (positive nucleic acid amplification test (NAAT+)/negative enzyme immunoassay (EIA−)) were included.Main outcome measures Treatment for CDI.Results CDI treatment was administered in 147 (71%) cases. …”
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Investigation and cause analysis of the psychological status of nursing staff under the normalization of epidemic prevention and control (常态化疫情防控期间护理人员心理状况调查及原因分析)... by ZHAO Jialu (赵佳璐), XIANG Wensheng (相文胜), WANG Huaxin (王华新), DONG Yuxia (董玉霞)

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RAPID AND SPESIFIC DETECTION OF MYCOBACTERIUM TUBERCULOSIS USING POLYMERASE CHAIN REACTION by Anita Kurniati, Desak Nyoman Surya Suameitra Dewi, Ni Nyoman Purwani

“…A specific, sensitive, rapid and cheap method for TB and other mycobacteria diagnosis in clinical specimen is a desperate needed in the laboratory diagnosis and hence management of tuberculosis. PCR as one of nucleic acid amplification assays have revolutionized MTB detection. …”
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Novel Fluorescent Dyes for Single DNA Molecule Techniques by Alexander Zarkov, Aleksey Vasilev, Todor Deligeorgiev, Stoyno Stoynov, Marina Nedelcheva-Veleva

“…To answer the demands of scientific and medical imaging issues, the family of nucleic acid fluorescent dyes is constantly enlarging. …”
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A Review on the Antimutagenic and Anticancer Effects of Cysteamine by Chun-Man Lee

“…Although the mechanisms of action are not fully understood, possible explanations are (i) free radical scavenging, (ii) alteration of the tumor cell proliferation by affecting nucleic acid and protein synthesis or inhibition of DNA synthesis, and (iii) hormone regulation. …”
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Imaging Gene Expression in Regional Brain Ischemia in Vivo with a Targeted [In]-Antisense Radiopharmaceutical by Toyofumi Suzuki, Yun Zhang, Yu-feng Zhang, Felix Schlachetzki, William M. Pardridge

“…The in vivo imaging of decreased GFAP gene expression in cerebral ischemia was examined in the present studies using a targeted peptide nucleic acid (PNA), which was labeled with 111 In, and which hybridized to nucleotides 20–37 of the rat GFAP mRNA. …”
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Novel Approaches to Diagnose COVID-19 by Solaf Jawhar Ali

“…SARS-CoV-2 can be detected with different methods in the laboratory including real time RT-PCR, chest CT scan and immunoassays. Viral nucleic acid testing has played important role in control COVIDI-19 outbreak.  …”
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Glycine rich proteins of ticks: more than a cement component by Renata Perotto de Souza, Mariana Vieira Dalla Valentina, Bruna Ferreira Leal, Sílvia Dias Oliveira, Carlos Alexandre Sanchez Ferreira, Ala Tabor

“…GRPs have been identified in a diverse array of organisms and have been shown to possess a number of distinctive biological characteristics, including nucleic acid binding, adhesive glue-like properties, antimicrobial activity, involvement in the stress response and in the formation of cuticle components. …”
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The quest for environmental analytical microbiology: absolute quantitative microbiome using cellular internal standards by Chunxiao Wang, Yu Yang, Xiaoqing Xu, Dou Wang, Xianghui Shi, Lei Liu, Yu Deng, Liguan Li, Tong Zhang

“…Abstract Background High-throughput sequencing has revolutionized environmental microbiome research, providing both quantitative and qualitative insights into nucleic acid targets in the environment. The resulting microbial composition (community structure) data are essential for environmental analytical microbiology, enabling characterization of community dynamics and assessing microbial pollutants for the development of intervention strategies. …”
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A Simple and Sensitive RT-qPCR Technology for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus by Hongri Zhao, Xingyu Xiao, Yajuan Sun, Yang Chen, Yongzhe Zhang, Peng Li, Hui Jin, Ying Li, Rui Yin

“…A simple and rapid RT-qPCR detection method for PRRSV was developed by optimizing nucleic acid amplification conditions. The results showed that the method was able to specifically detect PRRSV without cross-reactivity with the other 11 porcine susceptible viruses. …”
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A Novel Spontaneous Mutation of the SOX10 Gene Associated with Waardenburg Syndrome Type II by Sen Chen, Yuan Jin, Le Xie, Wen Xie, Kai Xu, Yue Qiu, Xue Bai, Hui-Min Zhang, Xiao-Zhou Liu, Xiao-Hui Wang, Wei-Jia Kong, Yu Sun

“…According to analysis based on nucleic acid and amino acid sequences, this mutation may produce a truncated protein, with loss of the HMG structure domain. …”
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