Importance of EQA/PT for the detection of genetic variants in comprehensive cancer genome testing by Kazuyuki Matsushita, Takayuki Ishige, Kousuke Watanabe, Toshiaki Akahane, Akihide Tanimoto, Michiko Yoshimoto, Munekazu Yamakuchi, Teruto Hashiguchi, Yoshinaga Okugawa, Makoto Ikejiri, Toshikazu Yamaguchi, Tadashi Yamasaki, Mayu Takeda, Masaaki Hibi, Naoki Akiyama, Kaho Shimizu, Naonori Hashimoto, Hiroko Sato, Yoshinori Tanaka, Fumie Amari, the EQA working group of Japan Association for Clinical Laboratory Science(JACLS)

“…This study revealed that preanalytical processes, such as derived nucleic acids (NA) extraction from formalin fixed paraffine embedded (FFPE) samples, are critical. …”
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Implementation and validation of a new qPCR assay to detect imported human Plasmodium species by Camille Cordier, Samia Hamane, Théo Ghelfenstein-Ferreira, Sarah Dellière, Élodie Da Silva, Blandine Denis, Sandrine Houzé, Valentin Joste, Alexandre Alanio

“…We have developed duplex (Plasmodium spp. and Plasmodium falciparum) real-time PCR (qPCR) and species-specific qPCR assays for the identification and quantification of human Plasmodium species. Whole nucleic acids from 523 samples prospectively collected from 410 patients suspected of malaria between June 2016 and March 2021 were tested by qPCRs and compared to standard diagnostic procedures. …”
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Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context by Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, Kaixiang Zhang, Jinghong Li

“…Rapid and inexpensive at-home testing of viral nucleic acids with single-base resolution remains a challenge. …”
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Active surveillance of avian influenza in the southwestern Poyang Lake area, China: Analyzing changes in wholesale and frozen fresh retail markets post-policy implementation by Wentao Song, Zhiqiang Deng, Fenglan He, Kang Fang, Lintao Sheng, Jingwen Wu, Junling Tu, Kun Zhou, Xi Wang, Wei Wang, Liu Yi, Kangguo Li, Buasiyamu Abudunaibi, Ping Zhang, Hui Li, Tianmu Chen

“…The type A avian influenza virus nucleic acids were detected using real-time reverse transcription polymerase chain reaction (real-time RT-PCR), and a triplex real-time RT-PCR assay kit specific for H5/H7/H9 RNA was utilized on the influenza A-positive samples to differentiate among the avian influenza virus subtypes. …”
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Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples by Camille d’Humières, Skerdi Haviari, Marie Petitjean, Laurène Deconinck, Signara Gueye, Nathan Peiffer-Smadja, Lynda Chalal, Naima Beldjoudi, Geoffrey Rossi, Yann Nguyen, Charles Burdet, Ségolène Perrineau, Diane Le Pluart, Roza Rahli, Michael Thy, Piotr Szychowiak, Xavier Lescure, Véronique Leflon-Guibout, Victoire de Lastours, Etienne Ruppé

“…Clinical metagenomics (CMg), i.e. the sequencing of a sample’s entire nucleic acids, may allow for the identification of bacteria not detected by conventional methods. …”
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The Laboratory Diagnosis of Neisseria gonorrhoeae by Lai-King Ng, Irene E Martin

“…Nucleic acid methods can also be used to detect the presence of low numbers in a specimen. …”
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Antiviral and Immunoenhancing Properties of 7-Thia-8-Oxoguanosine and Related Guanosine Analogues by Donald F Smee, Howard B Cottam, Brahma S Sharma, Ganesh D Kini, Ganapathi R Revankar, Emmanuel A Ojo-Amaize, Robert W Sidwell, Weldon B Jolley, Roland K Robins

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A drop dispenser for simplifying on-farm detection of foodborne pathogens. by Mohsen Ranjbaran, Simerdeep Kaur, Jiangshan Wang, Bibek Raut, Mohit S Verma

“…Nucleic-acid biosensors have emerged as useful tools for on-farm detection of foodborne pathogens on fresh produce. …”
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DNA replication initiation drives focal mutagenesis and rearrangements in human cancers by Pierre Murat, Guillaume Guilbaud, Julian E. Sale

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Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia by Hardian Susilo Addy, Norita Fatatik Azizi, Paniman Ashna Mihardjo

“…Moreover, two bacteriophages were obtained from this experiment (ϕRSSKD1 and ϕRSSKD2), which were able to infect all nine R. solanacearum isolates. Nucleic acid analysis showed that the nucleic acid of bacteriophages was DNA (deoxyribonucleic acid).…”
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An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-Reactivity with SARS Coronavirus by David M Patrick, Martin Petric, Danuta M Skowronski, Roland Guasparini, Timothy F Booth, Mel Krajden, Patrick McGeer, Nathalie Bastien, Larry Gustafson, Janet Dubord, Diane MacDonald, Samara T David, Leila F Srour, Robert Parker, Anton Andonov, Judith Isaac-Renton, Nadine Loewen, Gail McNabb, Alan McNabb, Swee-Han Goh, Scott Henwick, Caroline Astell, Jian Ping Guo, Michael Drebot, Raymond Tellier, Francis Plummer, Robert C Brunham

“…BACKGROUND: In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV).…”
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CRISPR/Cas12a-based method coupled with isothermal amplification to identify Alternaria spp. isolated from wheat grain samples by Aisha Shaizadinova, Aisha Shaizadinova, Meruyert Amanzholova, Meruyert Amanzholova, Irina Rukavitsina, Sailau Abeldenov, Anuar Rysbekovich Zhumakayev

“…Isothermal amplification methods, including nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA), enable nucleic acid amplification at constant temperatures, making them ideal for point-of-care diagnostics without the need for thermal cycling equipment. …”
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Investigation of west nile virus infection in brown bears (ursus arctos) in turkey by Yakup Yıldırım, Fethiye Çöven, Erdoğan Uzlu, Volkan Yılmaz, Emrah Çoban, Ayşegül Çoban, Çağan Hakkı Şekercioğlu

“…Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of West Nile Virus nucleic acid.<p> <b>Results:</b> Seropositivity rate was detected to be zero % and West Nile virus nucleic acid was not found in samples.…”
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The Gut Microbiota-Related Antihyperglycemic Effect of Metformin by Izabela Szymczak-Pajor, Józef Drzewoski, Małgorzata Kozłowska, Jan Krekora, Agnieszka Śliwińska

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The laboratory diagnosis of Chlamydia trachomatis infections by Max A Chernesky

“…Most invasive specimens, such as cervical or urethral swabs, may be collected for culture, antigen or nucleic acid detection. Noninvasive samples such as first-void urine and vaginal swabs can be easily collected by the patient; these samples must be tested by more sensitive nucleic acid amplification tests. …”
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Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis by Zahra Farshchian Yazdi, Shahrzad Roshannezhad, Samaneh Sharif, Mohammad Reza Abbaszadegan

“…Among diagnostic methods, those relying on nucleic acid detection are highly sensitive and specific. …”
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Ionizable polymeric micelles (IPMs) for efficient siRNA delivery by Ziyu Zhou, Yu Feng, Mingzhou Jiang, Zijun Yao, Jing Wang, Feng Pan, Rulan Feng, Chong Zhao, Yinyu Ma, Jinge Zhou, Lei Sun, Xiaotian Sun, Changyou Zhan, Xiao He, Kuan Jiang, Jiahui Yu, Zhiqiang Yan

“…Abstract Lipid nanoparticles (LNPs) are widely used for nucleic acid delivery but face challenges like limited targeting and accelerated blood clearance (ABC) effect. …”
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Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems by Xuebing Zhou, Qingcao Li, Zhanying Shi, Wenbo Lu, Chunhui Shu, Junyao Zhu, Yong Wu

“…During this experiment, viral nucleic acid was extracted directly from the samples without prior concentration. …”
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Raman Microspectral Study and Classification of the Pathological Evolution of Breast Cancer Using Both Principal Component Analysis-Linear Discriminant Analysis and Principal Compo... by Heping Li, Yu Ren, Fan Yu, Dongliang Song, Lizhe Zhu, Shibo Yu, Siyuan Jiang, Shuang Wang

“…Spectral analysis highlighted differences in levels of unsaturated and saturated lipids, carotenoids, protein, and nucleic acid between healthy and cancerous tissue and variations in the levels of nucleic acid, protein, and phenylalanine between DCIS and IDC. …”
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Children with COVID-19 Who Manifest Febrile Seizure by Lilia Dewiyanti, Neni Sumarni, Joseph Deni Lie, Zuhriah Hidajati, Harancang Pandih Kahayana, Adriana Lukmasari, Cipta Pramana

“…From the SARS-CoV-2 nucleic acid test, positive results were obtained on the third day of hospitalization. …”
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