Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas by Sandra L. Delgado, Piedad M. Perilla, Doris M. Salgado, María Clemencia Rojas, Carlos F. Narváez

“…The isolation of nucleic acids is a critical and limiting step for molecular assays, which prompted the arrival in Colombia of automated purification instruments during the SARS-CoV-2 pandemic. …”
Get full text

Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination by Nariman Zainali, Nariman Zainali, Houshang Alizadeh, Hassan Alizadeh, Philippe Delavault

“…Moreover, applying rNTPs and dNTPs, which mimic nitrogenous bases of nucleic acids, also caused a similar reduction in germination, suggesting that the non-specific inhibitory effect of the dsRNAs might arise from their nucleotides. …”
Get full text

Extracellular Vesicles: Role in Inflammatory Responses and Potential Uses in Vaccination in Cancer and Infectious Diseases by João Henrique Campos, Rodrigo Pedro Soares, Kleber Ribeiro, André Cronemberger Andrade, Wagner Luiz Batista, Ana Claudia Torrecilhas

“…Almost all cells and organisms release membrane structures containing proteins, lipids, and nucleic acids called extracellular vesicles (EVs), which have a wide range of functions concerning intercellular communication and signaling events. …”
Get full text

Roles for Exosomes in the Pathogenesis, Drug Delivery and Therapy of Psoriasis by Yuchao Chen, Huazhen Liu, Yuming He, Bin Yang, Weihui Lu, Zhenhua Dai

“…Especially, engineered exosomes may serve as drug delivery systems for effective delivery of proteins, nucleic acids or other drugs due to their low immunogenicity, good stability and ability to fuse with target cells. …”
Get full text

Specific Viruses Detected in Nigerian Children in Association with Acute Respiratory Disease by Oluwabukola M. Akinloye, Esa Rönkkö, Carita Savolainen-Kopra, Thedi Ziegler, Bamidele A. Iwalokun, Mope A. Deji-Agboola, Afolabi Oluwadun, Merja Roivainen, Festus D. Adu, Tapani Hovi

“…Validated real-time RT-PCR techniques revealed nucleic acids of at least one virus group in 189 specimens (77%). …”
Get full text

Positive Pneumocystis jirovecii Sputum PCR Results with Negative Bronchoscopic PCR Results in Suspected Pneumocystis Pneumonia by Kelly Pennington, John Wilson, Andrew H. Limper, Patricio Escalante

“…The diagnostic standard for Pneumocystis jirovecii pneumonia (PCP) is direct microscopic identification; however, in recent years, polymerase chain reaction (PCR) from bronchoalveolar lavage (BAL) samples to detect Pneumocystis nucleic acids has proven to be more sensitive and specific. …”
Get full text

The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels by Andrey A. Ayginin, Ekaterina V. Pimkina, Alina D. Matsvay, Anna S. Speranskaya, Marina V. Safonova, Ekaterina A. Blinova, Ilya V. Artyushin, Vladimir G. Dedkov, German A. Shipulin, Kamil Khafizov

“…We have subsequently designed a genus-specific oligonucleotide panel for targeted enrichment of viral nucleic acids in biological material and demonstrated the possibility of its application for virus detection in bird samples. …”
Get full text

New Insights in the Pathogenesis of HPV Infection and the Associated Carcinogenic Processes: The Role of Chronic Inflammation and Oxidative Stress by Simona Roxana Georgescu, Cristina Iulia Mitran, Madalina Irina Mitran, Constantin Caruntu, Maria Isabela Sarbu, Clara Matei, Ilinca Nicolae, Sandra Milena Tocut, Mircea Ioan Popa, Mircea Tampa

“…Reactive oxygen species exert a harmful effect on proteins, lipids, and nucleic acids. Viral oncogenes E5, E6, and E7 are involved in the development of chronic inflammation through various mechanisms. …”
Get full text

Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment by Artur Gurgul, Klaudia Pawlina-Tyszko, Monika Bugno-Poniewierska, Tomasz Szmatoła, Igor Jasielczuk, Stefan Dobosz, Konrad Ocalewicz

“…HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. …”
Get full text

Application of the weighted histogram method for calculating the thermodynamic parameters of the formation of oligodeoxyribonucleotide duplexes by I. I. Yushin, V. M. Golyshev, D. V. Pyshnyi, A. A. Lomzov

“…To date, many derivatives and analogs of nucleic acids (NAs) have been developed. Some of them have found uses in scientific research and biomedical applications. …”
Get full text

Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation by Viktoriya O. Samarskaya, Sofya Koblova, Tatiana Suprunova, Eugene A. Rogozhin, Nadezhda Spechenkova, Sofiya Yakunina, Andrew J. Love, Natalia O. Kalinina, Michael Taliansky

“…ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. …”
Get full text

Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles by Margarida Vaz, Tânia Soares Martins, Kevin Leandro, Luís Pereira de Almeida, Odete A. B. da Cruz e Silva, Alexandra Nunes, Ana Gabriela Henriques

“…Specifically, Aβ seems to cause alterations in protein phosphorylation and in the nucleic acids content of extracellular vesicles. These findings support that Aβ’s role in Alzheimer’s disease pathology may be mediated by extracellular vesicles and highlight FTIR’s potential for advancing Alzheimer’s disease research and clinical applications.…”
Get full text

Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…Modified primers (locked nucleic acids containing conformationally blocked nucleosides) providing a higher affinity to the DNA matrix and physico-chemical stability and a FAM-labeled TaqMan probe were selected for 5’-untranslatedregion of the genome. …”
Get full text

Emerging Strategies for Revascularization: Use of Cell-Derived Extracellular Vesicles and Artificial Nanovesicles in Critical Limb Ischemia by Vijay Murali Ravi Mythili, Ramya Lakshmi Rajendran, Raksa Arun, Vasanth Kanth Thasma Loganathbabu, Danyal Reyaz, ArulJothi Kandasamy Nagarajan, Byeong-Cheol Ahn, Prakash Gangadaran

“…They act as carriers of bioactive cargo, including proteins, nucleic acids, and lipids, that can modulate intricate cellular responses associated with ischemic tissue repair and angiogenesis. …”
Get full text

Metabolic profile of blood serum in experimental arterial hypertension by A. A. Seryapina, A. A. Malyavko, Yu. K. Polityko, L. V. Yanshole, Yu. P. Tsentalovich, A. L. Markel

“…To determine specific markers of diseases, various omics technologies are applied, including metabolomics, which makes it possible to evaluate the content of low-molecular compounds – amino acids, lipids, carbohydrates, nucleic acids fragments – in biological samples available for clinical analysis (blood and urine). …”
Get full text

Use of ascorbic acid (vitamin C) and alpha tocopherol (vitamin E) as adjuvants in the treatment of neuropathic pain by Fernando Antonio Silva de Azevedo-Filho, Yasmim Machado Chaves de Castro, Marcos Pinho Cerqueira, Thiago Arruda Rodrigues, Yussef Ali-Abdouni, Patrícia Maria de Moraes Barros Fucs

“…BACKGROUND AND OBJECTIVES: High concentrations of free radicals can damage nucleic acids, proteins and lipids and their accumulation may be involved in hypersensitivity to pain. …”
Get full text

Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs by Anna Schade, Paula Müller, Evgenya Delyagina, Natalia Voronina, Anna Skorska, Cornelia Lux, Gustav Steinhoff, Robert David

“…Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo. Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.…”
Get full text

Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer by Rebeca San Martin, Prijoyit Das, Tianchun Xue, Morgan Rose Brown, Renata Dos Reis Marques, Michael Essington, Adrian Gonzalez, Rachel Patton McCord

“…We also present modified protocols for subculturing cells and collecting nucleic acids and protein in high-calcium samples. Results: We find that prostate epithelial cell lines show increased adhesion and proliferation when cultured in these amorphous calcium surfaces, accompanied by independence from androgen starvation. …”
Get full text

Exosomal non-coding RNAs in the regulation of bone metabolism homeostasis: Molecular mechanism and therapeutic potential by Chengxiong Huang, Yu Xiao, Liming Qing, Juyu Tang, Panfeng Wu

“…Exos are rich in a variety of bioactive substances, such as non-coding RNAs, nucleic acids, proteins and lipids. In view of the structure of Exos, it can protect the biologically active molecules can be smoothly delivered to the target cells and involved in the regulation of cell function. …”
Get full text

Production of recombinant norovirus VP1 protein and its antigenic and immunogenic properties by Vladislav A. Lapin, Dmitry V. Novikov, Ekaterina V. Mokhonova, Dmitry A. Melentyev, Maria I. Tsyganova, Dmitry E. Zaitsev, Viktor V. Novikov

“…Computer analysis of nucleotide and amino acid sequences, molecular cloning, polymerase chain reaction, electrophoresis of nucleic acids in agarose gel and proteins in polacrylamide gel, affinity chromatography, enzyme immunoassay. …”
Get full text