Handgrip-Ring Structure Sensing Probe Assisted Multiple Signal Amplification Strategy for Sensitive and Label-Free Single-Stranded Nucleic Acid Analysis by Ying Ren, Yu He, Ping Li

“…Precise and efficient identification of single-stranded nucleic acids is crucial for both pathological research and early diagnosis of diseases, such as cancers. …”
Get full text

Diagnostic accuracy of cartridge-based nucleic acid amplification test and resistance pattern in new and previously treated tuberculosis cases: A prospective observational study by Ranjit Suva, Gaurang Khadiya, Kinjal D. Rami, Urvashi Modh, Firoz Ghanchi, Yogesh Murugan

Subjects: “…cartridge-based nucleic acid amplification test…”
Get full text

Tumor derived cell‐free nucleic acid upregulates programmed death‐ligand 1 expression in neutrophil via intracellular Toll‐like receptor signaling by Suguru Saito, Duo‐Yao Cao, Tomohiro Shibata, Yan Liu, Aoi Otagiri‐Hoshi, Xiaojiang Cui, Kenneth E. Bernstein

Get full text

A preliminary evaluation of a fast, low-cost, and high-throughput nucleic acid extraction method for bacterial microbiota profiling in low-microbial biomass samples by Xiangning Bai, Simon Rayner, Linda Ellevog Skuggen, Kine Kragl Engseth, Pål Marius Bjørnstad, Marianne Dalland, Gregor D. Gilfillan, Magnar Bjørås, Andreas Matussek

Subjects: Get full text

Evaluation of the performance of nucleic acid amplification tests (NAATs) in detection of chlamydia and gonorrhoea infection in vaginal specimens relative to patient infection status: a systematic review by Minttu M Rönn, Louise Mc Grath-Lone, Bethan Davies, Janet D Wilson, Helen Ward

“…Objective We evaluated the performance of nucleic acid amplification tests (NAATs) using vaginal specimens in comparison to specimens from the cervix or urine in their ability to detect chlamydia and gonorrhoea infection in women based on patient infection status (PIS).Design Systematic review.Data sources EMBASE and Ovid MEDLINE databases were searched through 3 October 2017.Eligibility criteria for selecting studies We included studies that tested samples from the vagina and ≥1 other site (cervix and/or urine) with ≥2 NAATs for chlamydia and ≥2 NAATs or 1 NAAT and culture for gonorrhoea for each site.Data extraction and synthesis Performance is defined as the sensitivity of a NAAT using a specimen site and PIS of the patient. …”
Get full text

Nucleic Acid Aptamers for Human Norovirus GII.4 and GII.17 Virus-Like Particles (VLPs) Exhibit Specific Binding and Inhibit VLPs from Entering Cells by Cheng C, Sun M, Li J, Xue Y, Cai X, Liu J, Wang X, Xu S, Xie Y, Zhang J

“…Here, we screened nucleic acid aptamers against the widespread HuNoV GII.4 and emerging HuNoV GII.17.Methods: After ten rounds of sieving for HuNoV GII.4 and GII.17 virus-like particles (VLPs), eight ssDNA aptamers were generated and characterized for each genotype.Results: Four of the eight aptamers generated for GII.4 VLP had dissociation constants (Kd) less than 100 nM, and all aptamers for GII.17 VLP had Kd less than 10 nM. …”
Get full text

Design and application of a bar-code scanning device for nucleic acid testing (一种核酸检测用辅助扫码装置设计与应用) by ZHANG Hongling (张红伶), YU Xinying (虞新颖), JIANG Hongli (蒋宏利)

Subjects: “…nucleic acid testing…”
Get full text

Evaluation of One Step Nucleic Acid Amplification for detection of lymph node metastases compared to histopathological ultrastaging in women with endometrial cancer: a protocol for a diagnostic accuracy study by Jan Kostun, Alessandra Mescalchin, Martin Pešta, Robert Slunečko, Khaled M. Ismail, Vendula Smoligová, Jiri Presl

“…Abstract Background This is a multicentre, European, prospective trial evaluating the diagnostic accuracy of One Step Nucleic Acid Amplification (OSNA) compared to sentinel lymph nodes histopathological ultrastaging in endometrial cancer patients. …”
Get full text

Assessing traditional Chinese medicine from a molecular perspective by Minhui Su, Wenxi Wang, Xudong Sun, Lihong Li, Weihong Tan

Subjects: Get full text

A Century-Old Threat: Disseminated Tuberculosis in a Healthy Host – Case Report by Poongodi Santhana Kumarasamy, Bala Ponnuthurai, Stanley James Benjamin Swamidoss, Mohamed Arafath Mohamed

Subjects: “…cartridge-based nucleic acid amplification test…”
Get full text

A prospective study to evaluate the clinical specificity of the cobas® MPX test kit for screening for HIV RNA, HCV RNA, and HBV DNA in blood donation samples using the cobas® 6800... by Lei Zhou, Lin Wang, Xiaofang Gong, Xiaochun Liu, Yaxuan Zou, Yingying Wang, Jinfeng Zeng, Liang Zang

Subjects: Get full text

Management modes and effects analysis of strengthening the volunteer’s normalized nucleic acid sampling (加强志愿者常态化核酸采样的管理方法与效果) by HAN Chunying (韩春英), YOU Chenggang (尤诚刚), PAN Meili (潘美丽), JIANG Yuting (蒋玉婷), XU Yiwen (徐伊雯), LU Weijie (陆玮婕), XIA Hui (夏卉), CHEN Haiyan (陈海燕), HUANG Ying (黄莹), GU Yuanlu (顾元露), CHEN Huiying (陈慧颖), ZHANG Qi (张琪), LI Xu (李旭), WU Weina (吴维娜), SHEN Xiaojie (沈晓洁), ZHOU Yi (周毅), ZHANG Xian (张宪), LI Qin (李琴)

Subjects: Get full text

Challenges in observing transcription–translation for bottom-up synthetic biology by Vadim G. Bogatyr, Gijs J. L. Wuite

Subjects: Get full text

Hepatitis E virus infection among blood donors in Zhengzhou by ZHAO Hongna, WEI Yueguang, YAN Lumin, TU Tiantian, WANG Shumin, WEI Yihui, WANG Yifang, ZHAO Lei, CHEN Mingjun

Subjects: Get full text

Gastroenterological Surgery and Management of Clostridioides difficile Infection: A Review by Tomonori Takano, Hiroyuki Aiba, Mitsuo Kaku, Hiroyuki Kunishima

Subjects: Get full text

Hepatitis E virus infection among blood donors in Ningbo by PENG Mingxi, LIU Yiyu, MAO Huyan, LIN Dan, XIN Lu, SHU Ning, HAN Jianfeng, DING Feng

Subjects: Get full text

Development of a Copro-RPA-CRISPR/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method. by Guoqing Shao, Xiaowei Zhu, Ruiqi Hua, Yanxin Chen, Guangyou Yang

Get full text

Development and validation of a LAMP-based method for rapid and reliable detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald by Moutoshi Chakraborty, Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Shamsul Arafin Bhuiyan, Simon Strachan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, Rebecca Ford, Rebecca Ford

Subjects: “…nucleic acid isolation…”
Get full text

Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy by Danhua Wang, Linlin Liu, Wenjing Chi, Zhenping Liu, Jiayun Wu, Yirou Liang, Fei He, Ruixiang Zhang, Pengxin Huang, Yunbo Li, Guangyu Qiu

Subjects: Get full text

Whole-genome amplification as a tool to improve bacterial detection by PCR in microbiological samples after endodontic treatment by Gabriela Ceccon Chianca, Gabriela Ceccon Chianca, Caroline Corrêa Fendeler, Caroline Corrêa Fendeler, Sérgio Pinto Chaves Junior, Sérgio Pinto Chaves Junior, Gabriella Lorena Dias Pereira, Helvécio Cardoso Corrêa Póvoa, Helvécio Cardoso Corrêa Póvoa, Helvécio Cardoso Corrêa Póvoa, Lívia Azeredo Alves Antunes, Lívia Azeredo Alves Antunes, Lívia Azeredo Alves Antunes, Leonardo Santos Antunes, Leonardo Santos Antunes, Leonardo Santos Antunes, Natalia Lopes Pontes Póvoa Iorio, Natalia Lopes Pontes Póvoa Iorio, Natalia Lopes Pontes Póvoa Iorio

Subjects: Get full text