Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data by Lihua Jiang, Liyi Pei, Ping Wang, Liqin Liu, Gong Li, Binjian Liu, Zhenming Lǚ, Tabata Hiromasa, Hao Pan, Atsushi Ogura

“…TLR13 is a TLR that localizes to endosomes and recognizes nucleic acids released by internal microorganisms, including viruses, bacteria, and fungi. …”
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The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels by Andrey A. Ayginin, Ekaterina V. Pimkina, Alina D. Matsvay, Anna S. Speranskaya, Marina V. Safonova, Ekaterina A. Blinova, Ilya V. Artyushin, Vladimir G. Dedkov, German A. Shipulin, Kamil Khafizov

“…We have subsequently designed a genus-specific oligonucleotide panel for targeted enrichment of viral nucleic acids in biological material and demonstrated the possibility of its application for virus detection in bird samples. …”
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Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination by Nariman Zainali, Nariman Zainali, Houshang Alizadeh, Hassan Alizadeh, Philippe Delavault

“…Moreover, applying rNTPs and dNTPs, which mimic nitrogenous bases of nucleic acids, also caused a similar reduction in germination, suggesting that the non-specific inhibitory effect of the dsRNAs might arise from their nucleotides. …”
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A bird’s-eye view of the biological mechanism and machine learning prediction approaches for cell-penetrating peptides by Maduravani Ramasundaram, Honglae Sohn, Thirumurthy Madhavan

“…Cell-penetrating peptides (CPPs) are highly effective at passing through eukaryotic membranes with various cargo molecules, like drugs, proteins, nucleic acids, and nanoparticles, without causing significant harm. …”
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Production of recombinant norovirus VP1 protein and its antigenic and immunogenic properties by Vladislav A. Lapin, Dmitry V. Novikov, Ekaterina V. Mokhonova, Dmitry A. Melentyev, Maria I. Tsyganova, Dmitry E. Zaitsev, Viktor V. Novikov

“…Computer analysis of nucleotide and amino acid sequences, molecular cloning, polymerase chain reaction, electrophoresis of nucleic acids in agarose gel and proteins in polacrylamide gel, affinity chromatography, enzyme immunoassay. …”
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Ribonuclease activity as a new prospective disease resistance marker in potato by E. A. Trifonova, S. M. Ibragimova, O. A. Volkova, V. K. Shumny, A. V. Kochetov

“…Higher plants possess a wide spectrum of enzymes catalyzing the hydrolysis of nucleic acids; it is believed that protection against pathogens is the most probable function of the enzymes. …”
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Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer by Xiaowei Cao, Zhenyu Wang, Liyan Bi, Jie Zheng

“…The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. …”
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Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles by Margarida Vaz, Tânia Soares Martins, Kevin Leandro, Luís Pereira de Almeida, Odete A. B. da Cruz e Silva, Alexandra Nunes, Ana Gabriela Henriques

“…Specifically, Aβ seems to cause alterations in protein phosphorylation and in the nucleic acids content of extracellular vesicles. These findings support that Aβ’s role in Alzheimer’s disease pathology may be mediated by extracellular vesicles and highlight FTIR’s potential for advancing Alzheimer’s disease research and clinical applications.…”
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Non-viral systems for intracellular delivery of genome editing tools by I. H. Shaikhutdinov, P. V. Ilyasov, O. V. Gribkova, L.  V. Limareva

“…Therefore, the search for novel approaches to delivering proteins and nucleic acids into cells is a relevant priority. This work reviews abiotic vectors and systems for delivering genome editing tools into target cells, including liposomes and solid lipid particles, other membrane-based vesicles, cell-penetrating peptides, micelles, dendrimers, carbon nanotubes, inorganic, polymer, metal and other nanoparticles. …”
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Characterization of Malignant Melanoma Using Vibrational Spectroscopy by Ziad Hammody, Ranjit Kumar Sahu, Shaul Mordechai, Emanuela Cagnano, Shmuel Argov

“…The comparative analysis of various parameters calculated from the spectral data of the normal epidermis and the abnormal regions showed that the changes in the nucleic acids was a significant indicator of the abnormal nature of the tissues. …”
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Electroporation of mouse embryonic stem cells with Neon device by I. P. Savchenkova, A. A. Savchenkova

“…Electroporation of mouse embryonic stem cells with the new-generation device can bean effective method for in vitro insertion of nucleic acids into the cells of interest to the researcher.…”
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CLARITY and Light-Sheet microscopy sample preparation in application to human cerebral organoids by T. A. Shnaider, I. E. Pristyazhnyuk

“…This method is based on a special sample preparation, during which lipids are removed from cells and replaced with hydrogel compounds such as acrylamide, while proteins and nucleic acids remain intact. CLARITY provides researchers with a unique opportunity to study three-dimensional biological structures while preserving their internal organization, including whole animals or embryos, individual organs and artificially grown organoids, in particular cerebral organoids. …”
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Construction of a pathogenic microorganism detection method based on third-generation nanopore sequencing data by Guoqin Mai, Jiayi Chen, Min Zhang, Wanyue Zhang, Yuting Luo, Ying Dai

“…First, a comprehensive and penetrating reference database of nucleic acids from pathogenic microorganisms was constructed, and sequence alignment analysis was performed via nanopore sequencing of metagenomic data collected from 40 patients with lower respiratory tract infection symptoms. …”
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Wastewater metagenomics in Africa: Opportunities and challenges. by Stephen Kanyerezi, Fatma Zahra Guerfali, Abbas Abel Anzaku, Oluwasegun Adesina Babaleye, Tracey Calvert-Joshua, Julien Alban Nguinkal, Oluwaseun Paul Amoo, Chiraz Atri, Waqasuddin Khan, Iqra Saleh, M Imran Nisar, Arthur Shem Kasambula, Koketso Morapedi, Gerald Mboowa

“…Utilizing high-throughput sequencing (HTS) technologies, this method allows for direct analysis of nucleic acids from wastewater samples, providing a cost-effective and comprehensive approach for pathogen surveillance. …”
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Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes by SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen

“…Additionally, the Fe + SP + L treatment led to increased lipid peroxidation and ROS production and decreased proportion of GSH, thereby damaging the integrity of the cell membrane, increasing the permeability of the cell membrane, decreasing the membrane potential, increasing the leakage of intracellular nucleic acids and proteins, and ultimately resulting in the death of bacteria. …”
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Regenerative activity of stomach epithelial cells at small-intestinal obstruction by V. Ye. Milyukov, K. K. Nguyen

“…Metabolic processes in cells of epithelium and stomach mucosal glands were studied by Einarson method of total nucleic acids detection. Test results were estimated by quantitative microspectrophotometry and computer software allowing to define the number of the area units of the study object occupied by cytochemical reaction products. …”
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Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens. by Nkechi Martina Odogwu, Jin Sung Jang, Sabrina Albertson, Clinton Hagen, Boyd Rasmussen, Oommen Saji, Timothy J Nelson, Wanek Program Preclinical Pipeline

“…Here, the efficiency of four RNA extraction methods: Qiagen AllPrep DNA/RNA method (Method QP); Qiagen AllPrep DNA/RNA method with protocol modification on the ethanol wash step after deparaffinization (Method QE); CELLDATA RNA extraction (Method BP) and CELLDATA RNA extraction with protocol modifications on the lysis step (Method BL) was compared on 23 matching FFPE cardiac tissue specimens (n = 92).In comparing RNA quality metrics across FFPE RNA extract, nucleic acids extracted deploying Method QE and QP produced the highest RNA yield. …”
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Use of ascorbic acid (vitamin C) and alpha tocopherol (vitamin E) as adjuvants in the treatment of neuropathic pain by Fernando Antonio Silva de Azevedo-Filho, Yasmim Machado Chaves de Castro, Marcos Pinho Cerqueira, Thiago Arruda Rodrigues, Yussef Ali-Abdouni, Patrícia Maria de Moraes Barros Fucs

“…BACKGROUND AND OBJECTIVES: High concentrations of free radicals can damage nucleic acids, proteins and lipids and their accumulation may be involved in hypersensitivity to pain. …”
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Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples by Camille d’Humières, Skerdi Haviari, Marie Petitjean, Laurène Deconinck, Signara Gueye, Nathan Peiffer-Smadja, Lynda Chalal, Naima Beldjoudi, Geoffrey Rossi, Yann Nguyen, Charles Burdet, Ségolène Perrineau, Diane Le Pluart, Roza Rahli, Michael Thy, Piotr Szychowiak, Xavier Lescure, Véronique Leflon-Guibout, Victoire de Lastours, Etienne Ruppé

“…Clinical metagenomics (CMg), i.e. the sequencing of a sample’s entire nucleic acids, may allow for the identification of bacteria not detected by conventional methods. …”
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Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas by Sandra L. Delgado, Piedad M. Perilla, Doris M. Salgado, María Clemencia Rojas, Carlos F. Narváez

“…The isolation of nucleic acids is a critical and limiting step for molecular assays, which prompted the arrival in Colombia of automated purification instruments during the SARS-CoV-2 pandemic. …”
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