Optimization of the DNA isolation procedure for assessing the genetic diversity of essential oil roses (<i>Rosa</i> L.) by S. B. Seitadzhieva, S. Z. Guchetl, S. V. Didovich, E. V. Gorodnyaya

“…High content of organic compounds that worsen nucleic acid purification in aromatic plants, as well as the use of such toxic substances as phenol and mercaptoethanol in many protocols for plant DNA isolation, make it advisable to take the above disadvantages into account when optimizing the DNA extraction technique for the work with essential oil rose plants. …”
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Anionic liposomes prepared without organic solvents for effective siRNA delivery by Xiu Han, Yan Lu, Zhaoluo Xu, Yanan Chu, Xueping Ma, Haiping Wu, Bingjie Zou, Guohua Zhou

“…Therefore, anionic liposomes prepared with glycerol will be a safe and effective delivery platform for siRNA and even other nucleic acid drugs.…”
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Expression of cytokeratin 19 in prostatic adenocarcinoma: a systematic review by Sina Shahshenas, Seyyed Mohammad Hosseini, Hossein Yarmohammadi, Masood Soltanipur, Mohammadreza Jalali Nadoushan

“…Eleven studies used reverse transcription polymerase chain reaction (RT-PCR) to investigate CK-19 expression, four used immunohistochemistry (IHC) staining, three used both one-step nucleic acid amplification (OSNA) and hematoxylin and eosin (H&E) methods, and three used the electrochemiluminescence (ECL) technique. …”
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Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies by Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Rita Lasickienė, Artiomas Akatov, Gabrielis Kundrotas, Vilimas Sereika, Raimundas Lelešius, Aurelija Žvirblienė, Kęstutis Sasnauskas

“…The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and inserted into yeast Saccharomyces cerevisiae expression plasmid. …”
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Evaluation of extracts from Phyllostachys makinoi for their antibacterial and accelerated wound healing potential by Te-Hua Liu, Ju-Yun Wu, Meng-Chun Cheng, Bang-Yuan Chen, Tsung-Yu Tsai

“…The extracts disrupted the cell membranes, causing electrolyte, protein, and nucleic acid leakage, leading to cell death. Additionally, P. makinoi extracts reduced lipopolysaccharide-induced nitric oxide, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 levels in RAW264.7 cells, and promoted wound healing by enhancing CCD-1112Sk cell proliferation. …”
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DNA-mediated self-assembly oxidative damage amplifier combined with copper and MTH1 inhibitor for cancer therapy by Cui Ren, Zhiyong Shi, Xiaowen Zhang, Xueer Yu, Yang Gao, Zhi Qi, Yu Chen, Yong Wang

“…Meanwhile, the released functional nucleic acid G3139 downregulated the expression of Bcl-2, and accelerated the apoptosis of tumor cells. …”
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Diabetic Polyneuropathy in Type 2 Diabetes Mellitus: Inflammation, Oxidative Stress, and Mitochondrial Function by Luis Miguel Román-Pintos, Geannyne Villegas-Rivera, Adolfo Daniel Rodríguez-Carrizalez, Alejandra Guillermina Miranda-Díaz, Ernesto Germán Cardona-Muñoz

“…These free radicals cause lipid peroxidation, protein modification, and nucleic acid damage, to finally induce axonal degeneration and segmental demyelination. …”
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Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma by Haideh Namdari, Farhad Rezaei, Fatemeh Heidarnejad, Mohammad Yaghoubzad-Maleki, Maryam Karamigolbaghi

“…In silico codon optimization and cloning of the vaccine nucleic acid sequence were accomplished using the pET28a plasmid. …”
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A Simple and Sensitive RT-qPCR Technology for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus by Hongri Zhao, Xingyu Xiao, Yajuan Sun, Yang Chen, Yongzhe Zhang, Peng Li, Hui Jin, Ying Li, Rui Yin

“…A simple and rapid RT-qPCR detection method for PRRSV was developed by optimizing nucleic acid amplification conditions. The results showed that the method was able to specifically detect PRRSV without cross-reactivity with the other 11 porcine susceptible viruses. …”
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Enterococcus durans SL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn (Triticum monococcum L. ssp. monococcum) by Berna Genc, Seyda Merve Karatas, Merve Tuğçe Tunç

“…This anionic heteropolysaccharide had, in addition to the carbohydrate backbone, a protein structure that did not contain nucleic acid. The carbohydrate backbone consisted of mannose, glucose, rhamnose, arabinose, xylose and galactose. …”
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Alternative splicing of modulatory immune receptors in T lymphocytes: a newly identified and targetable mechanism for anticancer immunotherapy by Shay Tzaban, Ori Stern, Elad Zisman, Galit Eisenberg, Galit Eisenberg, Shiri Klein, Shiri Klein, Shoshana Frankenburg, Michal Lotem, Michal Lotem, Michal Lotem

“…Two developments have accelerated the use of AS dynamics for therapeutic interventions: advancements in long-read RNA sequencing and progress in nucleic acid chemical modifications. Improved oligonucleotide stability has enabled their use in directing splicing to specific sites or modifying sequences to enhance or silence particular splicing events. …”
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A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9 by Akram Hosen, Md Nakibul Hasan Nishat, MD. Mohaimin Hossain Soaib, Omar Sharif Sharkar, Md. Sahabuddin, Imdadul Haque Sharif, Sujay Kumar Bhajan

“…ABSTRACT CRISPR Cas system is a revolutionary and precise genome editing system that induces bacterial and archaeal adaptive immunity and already has shown several promising applications in the treatment of genetic diseases and detection of nucleic acid targets. It is a complex tool, comprising diverse effector proteins and guide RNA that can be applied to dissociate and modify genes in living organisms along with the in vitro process. …”
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Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 by D. V. Gadzevich, S. I. Danylchenko, N. V. Vorotilova, M. A. Pasunkina, V. A. Uppe, V. N. Irza, M. S. Volkov, N. G. Zinyakov

“…The comparative genetic analysis of 258 bp nucleic acid sequences of the AIV H gene fragment showed that the identified isolate belongs to the Asian genetic lineage of highly pathogenic AIV subtype H5 (clade 2.3.4.4) associated with the epidemic spread in Asia, Europe, the Middle East and Africa in 2016–2020.…”
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Integrative analysis of transcriptome and metabolome profiling uncovers underlying mechanisms of the enhancement of the synthesis of biofilm in Sporobolomyces pararoseus NGR under... by Dandan Wang, Nan Zeng, Chunji Li, Chunwang Li, Yunjiao Wang, Bin Chen, Jiajia Long, Ning Zhang, Bingxue Li

“…Transcriptome and metabolome profiling indicated that carbohydrate metabolism, amino acid metabolism, lipid metabolism, and nucleic acid metabolism in S. pararoseus NGR cells were significantly enriched in biofilm cells under pH 4.7 culture conditions, including 56 differentially expressed genes and 341 differential metabolites. …”
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The effectiveness of community-wide screening for pulmonary tuberculosis: a systematic review by Susanna S. van Wyk, Ntombifuthi Blose, Lester Kapanda-Phiri, Mareli Claassens, Taryn Young

“…We found that sputum smear microscopy screening may result in little to no difference in the prevalence of culture-confirmed TB (n = 962,655, RR 1.09; 95% CI: 0.86–1.38, 1 RCT, low certainty evidence). Community-wide nucleic acid amplification test (NAAT) screening probably reduces the prevalence of NAAT-positive TB (n = 105,108, RR 0.56; 95% CI: 0.40–0.78, 1 RCT, moderate certainty evidence). …”
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INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform by Chun Ngo Yau, Jacky Tin Shing Hung, Robert A. A. Campbell, Thomas Chun Yip Wong, Bei Huang, Ben Tin Yan Wong, Nick King Ngai Chow, Lichun Zhang, Eldric Pui Lam Tsoi, Yuqi Tan, Joshua Jing Xi Li, Yun Kwok Wing, Hei Ming Lai

“…INSIHGT also allows multi-round, highly multiplexed 3D molecular probing and is compatible with downstream traditional histology and nucleic acid sequencing. With INSIHGT, we map undescribed podocyte-to-parietal epithelial cell microfilaments in mouse glomeruli and neurofilament-intensive inclusion bodies in the human cerebellum, and identify NPY-proximal cell types defined by spatial morpho-proteomics in mouse hypothalamus. …”
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Pathway Analysis of Metabolic Syndrome Using a Genome-Wide Association Study of Korea Associated Resource (KARE) Cohorts by Unjin Shim, Han-Na Kim, Yeon-Ah Sung, Hyung-Lae Kim

“…Through pathway analysis of MetS, pathways related with PDGF, mitogen-activated protein kinase, and PPAR signaling, as well as nucleic acid binding, protein secretion, and DNA repair, were identified. …”
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Cutting-edge: bionanomaterial solutions in the battle against Severe Acute Respiratory Syndrome Coronavirus 2 by U. Saeed, R. Uppal, M. H. Najmi, I. Fazal, A. A. Khan, Z. Z. Piracha, M. R. Uppal, H. N. Ijaz, D. U. Ozsahin, B. Uzun, I. Ozsahin

“…Their role in automating nucleic acid extraction and purification has proven indispensable, particularly in the diagnostic milieu of SARS-CoV-2. …”
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Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia by Babu Kanti Nath, Suman Das Gupta, Saranika talukder, Nasrin Sultana Tonu, Shane R. Raidal, Jade K. Forwood, Subir Sarker

“…Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high‐throughput sequencing at the Australian Genome Research Facility (AGRF). …”
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Molecular investigation of feline herpesvirus 1 (fhv-1) and feline calicivirus in cats with respiratory system problem by Seval Bilge Dağalp, Fırat Doğan, Touraj Aligholipour Farzani, Ali Rıza Babaoğlu, Gülizar Acar Kırmızı, Mehmet Çabalar

“…<p> <b>Materials and Methods:</b> For this purpose, a total of 119 specimens were collected from 70 from cats clinically showing respiratory system problem, 31 nasal, 30 conjunctival, 8 oral, 7 orafarengeal and 11 rectal swaps and 32 EDTA blood samples. Viral nucleic acid extraction was performed from the samples and the presence of these infections was investigated by PCR. …”
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