Prion meeting 2023: implications of a growing field by Tiago F. Outeiro, Tuane C. R. G. Vieira

“…John Stanley Griffith hypothesized that the agent causing these diseases was “probably a protein without nucleic acid” and, in 1982, Stanley Prusiner reported the identification of a proteinaceous infectious particle (coining the term prion) that was resistant to inactivation methods that were at the time standard for nucleic acids, and identified PrP as the major protein component of the infectious agent in scrapie and in Creutzfeldt-Jakob disease, classifying this also as a prion disease. …”
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The Role of Surveillance Inspections in Reducing False-Positives of SARS-CoV-2 Omicron Variants during the COVID-19 Epidemic by Li-Li Liu, Yu-Hong Zheng, You-Quan Zhang, Jin-Piao Lin, Zhi-Lin Luo, Liu-Min Yu, Shi-Hua Gao, Falin Chen

“…Daily surveillance inspection in laboratories can remind laboratories to strictly comply with standard procedures, focus on laboratory quality control, and reduce the occurrence of false-positive cases in SARS-CoV-2 nucleic acid tests to some extent. This study recommends that government decision-making departments establish policies and arrange experts to conduct daily surveillance inspections to improve laboratory quality control.…”
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Bacterial Diversity Associated with Terrestrial and Aquatic Snails by Konstantinos Apostolou, Canella Radea, Alexandra Meziti, Konstantinos Ar. Kormas

“…The steadfast technological progress in nucleic acid-based approaches would cause one to expect that scientific works on the microbial symbionts of animals would be balanced at least for the farmed animals of human interest. …”
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Analysis of the Effect of Female Genital Tuberculosis on Ovarian Reserve Parameters by Sharma Richa, Kanhere Anjali, Jain Sonal, Jain Akrati

“…Materials and Methods: A total of 133 infertile women who underwent diagnostic hysterolaparoscopy and cartridge-based nucleic acid amplification test testing of an endometrial biopsy were included in the study. …”
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Mycoplasma pneumoniae detections in children with acute respiratory infection, 2010–2023: a large sample study in China by Yuzhu Miao, Jungen Li, Linlin Huang, Ting Shi, Tingbo Jiang

“…Methods Children with ARTIs admitted to the Children’s Hospital of Soochow University (SCH) from January 2010 to December 2023 and underwent MP nucleic acid PCR assay were included. Clinical data on age, sex, onset time and detection result were collected and analyzed. …”
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Impact of deformed wing virus master variants (dwv-a, dwv-b, and dwv-c) in managed honey bee colonies of türkiye by Mustafa Emin Oz, Oguzhan Avci, Muge Dogan

“…<p> <b>Materials and Methods:</b> For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively.…”
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Purification and Characterization of Hyaluronic Acid Produced by Priestia flexa N7 by Dinh Giap Vu, Quang Tung Nguyen, Thi Cam Van Do, Thi Cuong Vu, Nguyen Phuong Dai Nguyen

“…Methods: The biochemical methods used in this study included Superdex™ G100 columns chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, protein and nucleic acid quantitation assays, and purification of HA. …”
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Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag by Enos C. Kline, Rose Duong, Qin Wang, Barry R. Lutz

“…These particles have biotechnological utility because of the ability to easily modify surface chemistry and compartmentalize nucleic acids or other materials. A consequential implementation of PLPs in diagnostics is as process controls in nucleic acid amplification tests, where control RNAs are packaged within the protein capsid and protected from degradation by RNases in the sample matrix. …”
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In Silico Design of a Trans-Amplifying RNA-Based Vaccine against SARS-CoV-2 Structural Proteins by Fatemeh Nafian, Ghazal Soleymani, Zahra Pourmanouchehri, Mahnaz Kiyanjam, Simin Nafian, Sayed Mohammad Mohammadi, Hanie Jeyroudi, Sharareh Berenji Jalaei, Fatemeh Sabzpoushan

“…Nucleic acid-based vaccines allow scalable, rapid, and cell-free vaccine production in response to an emerging disease such as the current COVID-19 pandemic. …”
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Role of Chest Computed Tomography versus Real Time Reverse Transcription Polymerase Chain Reaction for Diagnosis of COVID-19: A Systematic Review and Meta-Analysis by Dina M. Ali, Lamiaa G. Zake, Nevine K. El Kady

“…The diagnosis of COVID-19 depends on detection of the viral nucleic acid by real time reverse transcription polymerase chain reaction (RT-PCR). …”
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The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al-Najaf, Iraq by Aoula Al-Zebeeby, Ali Hadi Abbas, Haider Abas Alsaegh, Furkan Sabbar Alaraji

“…The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. …”
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Effectiveness of inactivated influenza vaccine against laboratory-confirmed influenza in elderly Chinese patients with diabetes: A test-negative design case-control study by Zhengyan Chen, Tianchi Yang

“…A total of 2,204 elderly patients were tested for influenza virus nucleic acid or antigen during the study period. After applying exclusion criteria, 199 influenza-positive cases and 199 matched influenza-negative controls were included in the final analysis. …”
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Study of some virulence genes from uropathogenic Klebsiella pneumoniae and Pseudomonas aeruginosa isolated in Wasit Province, Iraq by Rana H. Raheema, Sabaa Hilal Hadi, Zainab Adil Ghani Chabuck

“…When DNA was recovered from the isolates, the nucleic acid purity was diverse from 1.8 to 2.0, and the concentration fluctuated from 50 to 360 mg/µL. …”
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Molecular epidemiology of foot-and-mouth disease (review) by A. V. Scherbakov

“…These tests are based on constantly progressing technologies of nucleic acid sequencing and phylogenetic analysis. The use of these technologies made it possible to assess the genetic diversity of the FMD virus, to analyze the evolution of the virus in the enzootic regions, and track the pathways of FMD epizootic and panzootic spread. …”
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Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus by George Tetz, Kristina Kardava, Maria Vecherkovskaya, Alireza Khodadadi-Jamayran, Aristotelis Tsirigos, Victor Tetz

“…The results of this study add another line of evidence that the Universal Receptive System plays an important role in cell regulation, including cell responses to the environmental factors of clinically important pathogens, and that nucleic acid-based TezRs are functionally active parts of the extrabiome.…”
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Combination drug therapy prevents CIAKI by suppressing ER stress-induced apoptosis by Xuan Wang, Shan Han, Lili Zhao, Hongliang Cong

“…Among the five groups, Group PCC presented the significantly highest creatinine increase rate and protein and nucleic acid expression levels, with the most severe cell injury and apoptosis observed via HE and TUNEL staining. …”
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Repurposing of Drugs for Antibacterial Activities on Selected ESKAPE Bacteria Staphylococcus aureus and Pseudomonas aeruginosa by Bridget Kamurai, Molly Mombeshora, Stanley Mukanganyama

“…Curcumin caused protein and nucleic acid leakage from the bacterial cell membrane in both bacterial species. …”
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Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India by Rahul Chaurasia, Diptiranjan Rout, Shamsuz Zaman, Kabita Chatterjee, Hem Chandra Pandey, Abhishek Kumar Maurya

“…Background. Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies. …”
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CRISPR-Cas9 in basic and translational aspects of cancer therapy by Maryam Samareh Salavatipour, Zahra Poursalehi, Negin Hosseini Rouzbahani, Sohaib Mohammadyar, Mohammad Vasei

“…Introduction: The discovery of gene editing techniques has opened a new era within the field of biology and enabled scientists to manipulate nucleic acid molecules. CRISPR-Cas9 genome engineering has revolutionized this achievement by successful targeting the DNA molecule and editing its sequence. …”
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Research Progress in Small-Molecule Detection Using Aptamer-Based SERS Techniques by Li Zheng, Qingdan Ye, Mengmeng Wang, Fan Sun, Qiang Chen, Xiaoping Yu, Yufeng Wang, Pei Liang

“…Nucleic acid aptamers are single-stranded oligonucleotides that are selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries. …”
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