Discordant Clostridioides difficile diagnostic assay and treatment practice: a cross-sectional study in a tertiary care hospital, Geneva, Switzerland by Antoine Poncet, Laurent Kaiser, Lauriane Lenggenhager, Marie-Céline Zanella, Jacques Schrenzel

“…Objectives To determine the proportion of patients who received a treatment for Clostridioides difficile infection (CDI) among those presenting a discordant C. difficile diagnostic assay and to identify patient characteristics associated with the decision to treat CDI.Design Cross-sectional study.Setting Monocentric study in a tertiary care hospital, Geneva, Switzerland.Participants Among 4562 adult patients tested for C. difficile between March 2017 and March 2019, 208 patients with discordant tests’ results (positive nucleic acid amplification test (NAAT+)/negative enzyme immunoassay (EIA−)) were included.Main outcome measures Treatment for CDI.Results CDI treatment was administered in 147 (71%) cases. …”
Get full text

Aktivitas Daya Hambat Isolat Actinomycetes dengan Lama Fermentasi yang Berbeda terhadap Pertumbuhan Klebsiella pneumoniae by Annisa Nur Insani, Meiskha Bahar, Nunuk Nugrohowati, Retno Yulianti

“…Actinomycetes' inhibitory mechanism ranges from inhibition of cell wall synthesis to nucleic acid and protein synthesis. One Way ANOVA test showed significant differences between the inhibition zone produced by each fermentation group. …”
Get full text

Tree polynomials identify a link between co-transcriptional R-loops and nascent RNA folding. by Pengyu Liu, Jacob Lusk, Nataša Jonoska, Mariel Vázquez

“…R-loops are a class of non-canonical nucleic acid structures that typically form during transcription when the nascent RNA hybridizes the DNA template strand, leaving the non-template DNA strand unpaired. …”
Get full text

De novo design of protein minibinder agonists of TLR3 by Chloe S. Adams, Hyojin Kim, Abigail E. Burtner, Dong Sun Lee, Craig Dobbins, Cameron Criswell, Brian Coventry, Adri Tran-Pearson, Ho Min Kim, Neil P. King

“…Abstract Toll-like Receptor 3 (TLR3) is a pattern recognition receptor that initiates antiviral immune responses upon binding double-stranded RNA (dsRNA). Several nucleic acid-based TLR3 agonists have been explored clinically as vaccine adjuvants in cancer and infectious disease, but present substantial manufacturing and formulation challenges. …”
Get full text

Differential gene expression analysis of papillary thyroid carcinoma reveals important genes for lymph node metastasis by Wan Fahmi Wan Mohamad Nazarie, Azliana Mohamad Yusof, Francis Yew Fu Tieng, Nur Fadhlina Mohamad Pakarulrazy, Rohaizak Muhammad, Shahrun Niza Abdullah Suhaimi, Nani Harlina Md Latar, Sazuita Saidin, Isa Mohamed Rose, Learn-Han Lee, Nurul-Syakima Ab Mutalib

“…The biological processes of genes expressed at higher levels in PTC LNM compared to PTC LNN were involved in cell communication, energy pathway, and metabolism, whereas ion transport, energy pathways, and regulation of nucleobase, nucleoside, nucleotide, and nucleic acid metabolisms, were downregulated. Conclusion: These findings provide further evidence for the role of cellular transport regulatory processes in metastatic disease. …”
Get full text

Localisation of Abundant and Organ-Specific Genes Expressed in Rosa hybrida Leaves and Flower Buds by Direct In Situ RT-PCR by Agata Jedrzejuk, Heiko Mibus, Margrethe Serek

“…In situ PCR is a technique that allows specific nucleic acid sequences to be detected in individual cells and tissues. …”
Get full text

DNA MITOKONDRIA (mtDNA) SEBAGAI SALAH SATU PEMERIKSAAN ALTERNATIF UNTUK IDENTIFIKASI BAYI PADA KASUS INFANTISIDA by Taufik Hidayat

“…Penggunaan DNA (Deoxyribose Nucleic Acid) mitokondria atau mtDNA sebagai salah satu cara untuk mengetahui hubungan antara barang  bukti  medis  dengan  pelaku  berkembang  pesat  setelah  era  90an.  …”
Get full text

An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr by Atilla Yoldaş, Ramazan İlgün, Nevin Tuzcu, Akın Yığın, Erdal Özmen

“…<p> <b>Results:</b> The rabies virus specific nucleic acid was detected in masseter muscles motor nerves; N. mandibularis branch of N. trigeminus, N. masticatorius, motor leaf of N. mandibularis, N. massetericus and Nn. temporalis profunda which are two extra branches of N. masticatorius, unilaterally ganglion trigeminale, and pons by Real Time-PCR. …”
Get full text

Integrated and streamlined microfluidic device for molecular diagnosis of pathogen through direct PCR amplification by Dohwan Lee, Tae Seok Seo

“…However, the complexity of the PCR process (including nucleic acid extraction/purification, amplification, and amplicon detection) hinders its widespread adoption in point-of-care testing, where simplicity and rapidity are essential for practical use. …”
Get full text

Supramolecular Biopolymers for Tissue Engineering by Rosario Pérez-Pedroza, Alan Ávila-Ramírez, Zainab Khan, Manola Moretti, Charlotte A. E. Hauser

“…In this review, we will analyse the 3 subtypes of SBPs: peptide-, nucleic acid-, and oligosaccharide-derived. Then, we will discuss the role that SBPs will be playing in TE as dynamic scaffolds, therapeutic scaffolds, and bioinks. …”
Get full text

Research on the Cross-Regional Traveling Welcome Short Messaging Service During the COVID-19 Pandemic: A Survey from Mobile Users’ Perspective by Zhiyuan Yu, Chi Zhang

“…Reminders about traveling registration and nucleic acid testing were assigned the highest importance, as they offer convenience in communicating the most up-to-date prevention and control information to mobile users during intercity travel. …”
Get full text

Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens by Govindan Muthukumar, Ayyanar Kamalakannan, Irudhayasamy Johnson, Pachamuthu Kamaraj, Iyyamperumal Muthuvel, Shanmugam Varanavasiappan

“…Age-old management practices are time-consuming and labour-intensive processes. In the past, nucleic acid-based methods, such as hybridization, amplification, and sequencing, have been used extensively for the preliminary identification of plant pathogens. …”
Get full text

Transcytosis of T4 Bacteriophage Through Intestinal Cells Enhances Its Immune Activation by Amanda Carroll-Portillo, October Barnes, Cristina N. Coffman, Cody A. Braun, Sudha B. Singh, Henry C. Lin

“…Interactions between bacteriophages with mammalian immune cells are of great interest and most phages possess at least one molecular pattern (nucleic acid, sugar residue, or protein structure) that is recognizable to the immune system through pathogen associated molecular pattern (PAMP) receptors (i.e., TLRs). …”
Get full text

A Small Surge in Incidence of SARS-CoV-2 Omicron Variant in the “Dynamic Zero” Period by Xiaona Li, Ruili Li, Qiguo Lian, Yang Wang, Wenkui Gu, Qinghe Meng

“…The low reproduction number and the Omicron outbreak being suppressed within a short time marked the effectiveness of the implemented public health measures, such as nucleic acid screening, social distancing, masking, vaccination, medical treatment of patients, and isolation of close contacts. …”
Get full text

Gene Editing in Pluripotent Stem Cells and Their Derived Organoids by Hang Zhou, Yun Wang, Li-Ping Liu, Yu-Mei Li, Yun-Wen Zheng

“…Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research and development boom with significant implications in regenerative medicine. …”
Get full text

Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates by Amin Hadi, Abbas Rastgoo, Maryam Eskandarian, Nooshin Haghighipour, Azam Bolhassani

“…Cell viability and low nucleic acid expression are the problems related to dendritic cells (DCs) and mesenchymal stem cells (MSCs), which are transfected with plasmid DNA. …”
Get full text

Analysis of multidrug resistant group B streptococci with reduced penicillin susceptibility forming small, less hemolytic colonies. by Hirotsugu Banno, Kouji Kimura, Yosuke Tanaka, Tsuyoshi Sekizuka, Makoto Kuroda, Wanchun Jin, Jun-Ichi Wachino, Keiko Yamada, Keigo Shibayama, Yoshichika Arakawa

“…We identified a 276_277insG nucleic acid insertion in the thiamin pyrophosphokinase (tpk) gene, resulting in premature termination at amino acid 103 in TPK, as a candidate mutation responsible for small colony formation. …”
Get full text

Role of ixodid ticks in tick-borne pathogen spread and circulation in the Belarusian Lakeland by A. A. Osmolovsky, I. A. Subotsina

“….), Babesia spp. and Tick-borne encephalitis virus genetic materials with real-time polymerase chain reaction using the reagent kit for nucleic acid extraction from environmental samples in accordance with the manufacturer’s instruc­tions. …”
Get full text

The Psu protein of phage satellite P4 inhibits transcription termination factor ρ by forced hyper-oligomerization by Daniela Gjorgjevikj, Naveen Kumar, Bing Wang, Tarek Hilal, Nelly Said, Bernhard Loll, Irina Artsimovitch, Ranjan Sen, Markus C. Wahl

“…ATPase, nucleotide binding and nucleic acid binding studies revealed that Psu hinders ρ ring closure and traps nucleotides in their binding pockets on ρ. …”
Get full text

Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China by Rui Zhao, Guilun He, Lin Xiang, Melinda Ji, Rongheng He, Xudong Wei

“…Direct identification of infected tissues is currently the primary method of diagnosis; however, the low sensitivity and inconvenience of microscopic examination in detecting amastigotes, parasitic manifestations of Leishmania, leads to the possibility of misdiagnosis, delayed diagnosis, and underdiagnosis.MethodsWith the development of metagenomic nextgeneration sequencing (mNGS) technology for pathogen identification, it is possible to detect specific nucleic acid sequences characteristic of Leishmania parasites, which opens new avenues for the more accurate diagnosis of leishmaniasis. …”
Get full text