Protein-DNA interactions: statistical analysis of interatomic contacts in major and minor grooves by A. A. Anashkina, E. N. Kuznetsov, A. V.  Batianovskii, L. A. Uroshlev, V. G. Tumanyan, N. G. Esipova

“…Analysis of the accessible surface area of atoms in the major and the minor grooves showed a correlation with the number of contacts (coefficient of linear correlation 0.94 and 0.93, respectively), however, nucleic acid atoms forming hydrogen bonds make contacts more often than may be expected from statistical considerations. …”
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Prevalence and Residual Risk of HIV in Volunteer Blood Donors of Zhejiang Province, China, from 2018 to 2022 by Hong Zhu, Wei Ding, Wenjuan Han, Xiaofan Zheng, Yiqing Hu, Jie Dong, Yaling Wu, Danxiao Wu, Jinhui Liu, Faming Zhu

“…Blood safety levels have been significantly improved since the implementation of nucleic acid amplification technology (NAT) testing for blood donors. …”
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Practical Experience of Endoscope Reprocessing and Working-Platform Disinfection in COVID-19 Patients: A Report from Guangdong China during the Pandemic by Xianqi Lin, Zhenyi Zhang, Minzhao Gao, Zhenling Zhang, Zhidong Lin, Siwen Huang, Jiangnan Ren, Meijuan Luo, Xiaoqing Xu, Guilan Chen, Hongjun Chen, Renxu Lai, Xiaochun Wu, Lihua Zhang, Xiaofeng Li

“…For endoscopic preprocessing at the bedside, a key disinfection step is using a multienzyme stock solution. The nucleic acid tests for endoscopists, washers, endoscopes, and working platforms were all negative. …”
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Altered O-Glycans in stimulated whole saliva from patients with primary Sjögren’s syndrome and non-pSS sicca by Sarah Kamounah, Kristina A. Thomsson, Christiane Elisabeth Sørensen, Eric Paul Bennett, Niclas G. Karlsson, Anne Marie Lynge Pedersen

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Examining the Formalin Fixation Method for Maintaining High RNA Quality in Surgical Lung Specimens by Takashi Teishikata, Manabu Itoh, Yusuke Okamoto, Naofumi Miyahara, Chiho Nakashima, Koichiro Takahashi, Masafumi Hiratsuka, Keita Kai, Keiji Kamohara

“…No significant difference was observed in nucleic acid concentration. The multiplex genetic analysis success rate was 95% with conventional methods (one failure); however, it was 100% with the sheet‐like fixation method. …”
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Performance evaluation of the Qiagen BK virus ASR on the NeuMoDx system by Amorce Lima, Luciano Soares, Caroline Simmons, Laura Rowe, Dominic Uy, Deanna Becker, Suzane Silbert

“…Methods: The analytical performance was assessed using commercially available BKV Panels that meet the 1st WHO International Standards for BKV nucleic acid amplification techniques. The clinical performance was evaluated using 204 residual plasma and urine samples previously identified with the SOC assay. …”
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A Novel Spontaneous Mutation of the SOX10 Gene Associated with Waardenburg Syndrome Type II by Sen Chen, Yuan Jin, Le Xie, Wen Xie, Kai Xu, Yue Qiu, Xue Bai, Hui-Min Zhang, Xiao-Zhou Liu, Xiao-Hui Wang, Wei-Jia Kong, Yu Sun

“…According to analysis based on nucleic acid and amino acid sequences, this mutation may produce a truncated protein, with loss of the HMG structure domain. …”
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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

“…The use of glycerol as a phase separator and the direct release of nucleic acids from the virus by NaOH significantly enhanced the stability of the assay and reduced its economic cost. …”
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Advancements in functional tetrahedral DNA nanostructures for multi-biomarker biosensing: Applications in disease diagnosis, food safety, and environmental monitoring by Yun Qiu, Yixing Qiu, Wenchao Zhou, Dai Lu, Huizhen Wang, Bin Li, Bin Liu, Wei Wang

“…This review highlights recent advancements in functional tetrahedral DNA nanostructures (FTDN) for various biomarkers monitoring, including nucleic acid, protein, mycotoxin, agent, and metal ion. …”
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Clade 2.3.4.4b but not historical clade 1 HA replicating RNA vaccine protects against bovine H5N1 challenge in mice by David W. Hawman, Thomas Tipih, Eddie Hodge, E. Taylor Stone, Nikole Warner, Natalie McCarthy, Brian Granger, Kimberly Meade-White, Shanna Leventhal, Kiara Hatzakis, Stephanie Park, Karen Gaffney, Kyle Rosenke, Jesse H. Erasmus, Heinz Feldmann

“…Our data highlight the utility of nucleic acid vaccines to be rapidly updated to match emergent viruses of concern while demonstrating that contemporary bovine H5N1 viruses can evade immunity elicited by historical HA antigens.…”
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Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya by Kinyatta Nancy, Wambua Lillian, Mutahi Wilkinson, Mugasa Claire, Kamau Luna, Wachira Dorcas, Githae Rosemary, Lusweti Japheth, Ichugu Christine, Waigi Emily, Kagai Jim

“…Immunological methods to detect infection are available; however, cross-reactivity issues have been reported in most of them. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity and can be used to detect the infections. …”
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Evaluation of a new human immunodeficiency virus antigen and antibody test using light-initiated chemiluminescent assay by Yijun Li, Fangfang Jin, Yunhui Li, Yan Li, Yajie Wang, Ximing Yang

“…Screening-reactive results were confirmed by Western blotting and nucleic acid testing.ResultsTotal imprecision was within 2.49%–6.56%. …”
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Isolation and Characterization of Coronavirus and Rotavirus Associated with Calves in Central Part of Oromia, Ethiopia by Umer Seid, Fufa Dawo, Asamino Tesfaye, Munera Ahmednur

“…At the molecular stage, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to determine the presence of coronavirus and rotavirus nucleic acid by using specific primers. The 5 samples that were coronavirus and rotavirus antigen positive by ELISA and develop CPE on cell culture were also positive on RT-PCR technique. …”
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The Escalating Magnitude of COVID-19 Infections among the Northeastern Ethiopia Region: A Community-Based Cross-Sectional Study by Zeleke Geto, Saba Gebremichael, Melaku Ashagrie Belete, Alemu Gedefie, Genet Molla, Melkam Tesfaye, Wondmagegn Demsiss, Daniel Gebretsadik

“…After extraction of viral nucleic acid from oropharyngeal specimen, the real-time fluorescent polymerase chain reaction (RT-PCR) kit was used for detecting novel coronavirus. …”
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The effect of SARS-CoV-2 Omicron BA. 5.2 infection on perioperative physiological indicators in orthopedic patients by Hao Wu, JunZhe Yang, YuQin Yan, Xiao Hu, BiXi Li, PengFei Cheng, XiaoYang Song

“…Methods Patients who underwent time-limited surgery (surgery that must be performed within a certain time) at the Department of Orthopedics of our hospital from September 1, 2022, to March 31, 2023, were divided into an observation group (those with Omicron BA 5.2 and a negative nucleic acid test after December 7, 2022, n = 100) and a control group (those negative for SARS-CoV-2 infection before December 7, 2022, n = 100). …”
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Analytic and clinical validity of thyroid nodule mutational profiling using droplet digital polymerase chain reaction by Vincent L. Biron, Ashlee Matkin, Morris Kostiuk, Jordana Williams, David W. Cote, Jeffrey Harris, Hadi Seikaly, Daniel A. O’Connell

“…Droplet digital PCR (ddPCR) is an ultrasensitive method of nucleic acid detection that is particularly useful for identifying gene mutations. …”
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Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China by Le Chang, Junpeng Zhao, Fei Guo, Huimin Ji, Lu Zhang, Xinyi Jiang, Lunan Wang

“…All plasma samples were tested by eight ELISA assays in 16 blood centers, followed by the detection of CLIA and ECLIA methods in the National Center for Clinical Laboratories (NCCL), further confirmed by nucleic acid testing (NAT) and Western blot (WB). Results. …”
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Urine proteomics defines an immune checkpoint-associated nephritis signature by Cassian Yee, Jamie S Lin, James P Long, Shailbala Singh, Yanlan Dong

“…However, identification of effective biomarkers of disease using established technologies such as proximity ligation assays (PLA) and bead-based immunoassays is challenging due to their limited sensitivity and loss of precision in multiplex analysis.To address this, we employed cutting-edge NUcleic acid Linked Immuno-Sandwich Assay (NULISA) technology to measure protein expression in urine and plasma samples from AKI patients undergoing ICI therapy. …”
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MicroRNA-3145 as a potential therapeutic target for hepatitis B virus: inhibition of viral replication via downregulation of HBS and HBX by Amrizal Muchtar, Amrizal Muchtar, Daichi Onomura, Daichi Onomura, Dan Ding, Dan Ding, Hironori Nishitsuji, Kunitada Shimotohno, Shunpei Okada, Keiji Ueda, Koichi Watashi, Takaji Wakita, Kei Iida, Kei Iida, Hironori Yoshiyama, Hisashi Iizasa

“…Current treatments for hepatitis B virus (HBV), such as interferons and nucleic acid analogs, have limitations due to side effects like depression and the development of drug-resistant mutants, highlighting the need for new therapeutic approaches. …”
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Comparative evaluation of cell-based assay technologies for scoring drug-induced condensation of SARS-CoV-2 nucleocapsid protein by Rui Tong Quek, Cyna R. Shirazinejad, Christina L. Young, Kierra S. Hardy, Samuel Lim, Phillip J. Elms, David T. McSwiggen, Timothy J. Mitchison, Pamela A. Silver

“…Protein-nucleic acid phase separation has been implicated in many diseases such as viral infections, neurodegeneration, and cancer. …”
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