Leveraging Next-Generation Sequencing Application from Identity to Purity Profiling of Nucleic Acid-Based Products by Rucha Wadapurkar, Swarda Deo, Renuka Khanzode, Ajay Singh

“…They represent an emerging field; thus, their quality control and regulatory landscape is evolving to ensure adequate quality and safety. Next-Generation Sequencing (NGS) is mostly recommended for NAP identity testing, and we are leveraging its application for impurity profiling. …”
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Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide. by Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

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MaterniCode: New Bioinformatic Pipeline to Detect Fetal Aneuploidies and Rearrangements Using Next-Generation Sequencing by Federico Gabrielli, Filomena Tiziana Papa, Fabio Di Pietro, Andreu Paytuví-Gallart, Daniel Julian, Walter Sanseverino, Cinzia Alfonsi

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Next generation sequencing uncovers multiple miRNAs associated molecular targets in gallbladder cancer patients by Rahul Saxena, Baskar Chakrapani, M. P. Sarath Krishnan, Amit Gupta, Sweety Gupta, Jayanta Das, Subash C. Gupta, Anissa A. Mirza, Shalinee Rao, Bela Goyal

“…However, many patients are ineligible for surgery, and current adjuvant treatments exhibit limited effectiveness. Next-generation sequencing has improved our understanding of molecular pathways in cancer, sparking interest in microRNA-based gene regulation. …”
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Metagenomic next-generation sequencing for lung cancer low respiratory tract infections diagnosis and characterizing microbiome features by Yao Liu, Bohan Yang, Qi Qi, Shijie Liu, Yiheng Du, Linlin Ye, Qiong Zhou

Subjects: “…metagenomic next-generation sequencing (mNGS)…”
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Application of Next-Generation Sequencing Technology Based on Single Gene Locus in Species Identification of Mixed Meat Products by Xinmei Liu, Zhiyang Liu, Yiyu Cheng, Haijing Wu, Wei Shen, Yan Liu, Qiushi Feng, Jun Yang

“…This study aimed to test whether next-generation sequencing (NGS) technology could be used as a method for the certification of mixed meat products. …”
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Genome Sequencing of Hericium coralloides by a Combination of PacBio RS II and Next-Generation Sequencing Platforms by Caixia Zhang, Lijun Xu, Jian Li, Jiansong Chen, Manjun Yang

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Cancer treatment comes to age: from one-size-fits-all to next-generation sequencing (NGS) technologies by Sepideh Parvizpour, Hanieh Beyrampour-Basmenj, Jafar Razmara, Farhad Farhadi, Mohd Shahir Shamsir

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FastContext: A tool for identification of adapters and other sequence patterns in next generation sequencing (NGS) data by E. Viesná, V. Fishman

Subjects: “…next generation sequencing…”
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The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing by Ping Sun, Zhiyong Guo, Daiping Guo, Jian Wang, Tingting Wu, Tingjun Li, Jiannan Liu, Xinhua Liu

“…Then, the V3-V4 hypervariable regions of the 16S rRNA gene were amplified and subjected to next-generation sequencing. The statistical analysis was performed by R software (V3.5.1). …”
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A walk through the development of human leukocyte antigen typing: from serologic techniques to next-generation sequencing by Haeyoun Choi, Eun-Jeong Choi, Hyoung-Jae Kim, In-Cheol Baek, Aegyeong Won, Su Jin Park, Tai-Gyu Kim, Yeun-Jun Chung

Subjects: “…human leukocyte antigen; histocompatibility testing; transplantation; next-generation sequencing…”
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Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage by Rob E. Carpenter, Vaibhav Tamrakar, Harendra Chahar, Tyler Vine, Rahul Sharma

“…Rapid identification and tracking of emerging SARS-CoV-2 variants are critical for understanding the transmission dynamics and developing strategies for interrupting the transmission chain. Next-Generation Sequencing (NGS) is an exceptional tool for whole-genome analysis and deciphering new mutations. …”
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Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation by Olivia Ott, Sabrina Tolppi, Jennifer Figueroa-Cruz, Khaliun Myagmar, Khulan Unurbuyan, Anubhav Tripathi

“…Abstract Next-generation sequencing (NGS) is emerging as a powerful tool for molecular diagnostics but remains limited by cumbersome and inefficient sample preparation. …”
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Accelerating next generation sequencing data analysis: an evaluation of optimized best practices for Genome Analysis Toolkit algorithms by Karl R. Franke, Erin L. Crowgey

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Metagenomic Next-Generation Sequencing (mNGS) for the Timely Diagnosis of Carbapenem-Resistant Klebsiella pneumoniae in Leukemia Patients by Huan Tao, Fang Hua, Yingying Chen, Yongqian Jia

“…This report shows the contribution of metagenomic next-generation sequencing (mNGS) as an alternative to challenging diagnostic infection in immunosuppressed individuals. …”
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Efficiency to Discovery Transgenic Loci in GM Rice Using Next Generation Sequencing Whole Genome Re-sequencing by Doori Park, Dongin Kim, Green Jang, Jongsung Lim, Yun-Ji Shin, Jina Kim, Mi-Seong Seo, Su-Hyun Park, Ju-Kon Kim, Tae-Ho Kwon, Ik-Young Choi

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Comparison of molecular analysis results determined by next-generation sequencing to immunohistochemical indicators and clinicopathological parameters in prostate adenocarcinomas by Merve Çırak Balta, İbrahim Halil Erdoğdu, Esin Oktay, Nil Çulhac

Subjects: “…next-generation sequencing…”
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Utilizing Targeted Next-Generation Sequencing for Rapid, Accurate, and Cost-Effective Pathogen Detection in Lower Respiratory Tract Infections by Qin L, Liang M, Song J, Chen P, Zhang S, Zhou Y, Li H, Tang J, Ma Y, Yang B, Xu J, Zhang J

Subjects: “…targeted next-generation sequencing…”
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Embryo Mosaicism Rate in National Referral Hospital of Indonesia Detected Using Next-Generation Sequencing: A Retrospective Study by Achmad Kemal Harzif, Muhammad Ikhsan, Pritta Ameilia Iffanolida, Kresna Mutia, Budi Wiweko, R Muharam, Kanadi Sumapraja, Gita Pratama, Mila Maidarti, Vita Silvana, Amalia Shadrina, Irene Sinta Febriana, Nafi'atul Ummah, Aisyah Retno Puspawardani, Andon Hestiantoro

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Clinical characteristics of pathogens in children with community-acquired pneumonia were analyzed via targeted next-generation sequencing detection by Junhua Zhao, Mingfeng Xu, Zheng Tian, Yu Wang

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