Eight Years of Norovirus Surveillance in Urban Wastewater: Insights from Next-Generation by Giusy Bonanno Ferraro, David Brandtner, Pamela Mancini, Carolina Veneri, Marcello Iaconelli, Elisabetta Suffredini, Giuseppina La Rosa

“…A total of 337 wastewater samples were analyzed using RT-nested PCR and next-generation sequencing (NGS) to identify genogroups GI and GII and their respective genotypes. …”
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Electroencephalographic and Epilepsy Findings in ZNF711 Variants: A Case Series of Two Siblings by Michele Minerva, Lorenzo Perilli, Samanta Carbone, Margherita Maria Rossi, Federica Lotti, Luisa Lonoce, Maria Rosaria Curcio, Salvatore Grosso

“…Both children underwent various genetic testing and counselling, including an extended next-generation sequencing (NGS) panel, revealing a hemizygous c.657C > G pathogenic variant in the ZNF711 gene from maternal inheritance. …”
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Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study by Birgit Henrich, Ilka Hermann, Mara Di Giulio, Karl Köhrer, René Deenen, Sugi Sivalingam, Ulrike Peters, Thomas Beikler, Ralf Janda, Stefan Rüttermann

“…In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed. Results. In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST. …”
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Analysis of a Family with Brugada Syndrome and Sudden Cardiac Death Caused by a Novel Mutation of SCN5A by Yao-Bin Zhu, Jian-Hui Zhang, Yuan-Yuan Ji, Ya-Nan Hu, Han-Lu Wang, Dan-Dan Ruan, Xiao-Rong Meng, Xin-Fu Lin, Jie-Wei Luo, Wei Chen

“…Three generations of a Han Chinese family with Brugada syndrome were recruited in the study; their clinical phenotype data were collected and DNA samples extracted from the peripheral blood. Next-generation sequencing was carried out in the proband, and candidate genes and mutations were screened using the full exon capture technique. …”
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De Novo Genome Assembly for an Endangered Lemur Using Portable Nanopore Sequencing in Rural Madagascar by Lindsey Hauff, Noa Elosmie Rasoanaivo, Andriamahery Razafindrakoto, Hajanirina Ravelonjanahary, Patricia C. Wright, Rindra Rakotoarivony, Christina M. Bergey

“…While the advent of next‐generation sequencing technologies and the resulting reduction in associated costs have led to the proliferation of genomic data and high‐quality reference genomes, global discrepancies in genomic sequencing capabilities often result in biological samples from biodiverse host countries being exported to facilities in the Global North, creating inequalities in access and training within genomic research. …”
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Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer by V. G. Druzhinin, E. D. Baranova, P. S. Demenkov, L. V. Matskova, A. V. Larionov

“…In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. …”
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Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep by Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A. Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

“…The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. …”
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Immunolipid magnetic bead-based circulating tumor cell sorting: a novel approach for pathological staging of colorectal cancer by Qingyan Deng, Weidong Li, Yueming Huang, Haitao Wang, Xinhao Zhou, Zhifen Guan, Bohao Cheng, Yao Wang

“…Hotspot mutations in patient tissue samples were identified via next-generation sequencing (NGS), whereas mutations in blood CTCs were detected via Sanger sequencing. …”
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Genetic Characteristics of the Rat Fibroblast Cell Line Rat-1 by Thomas Liehr, Stefanie Kankel, Eva Miriam Buhl, Sarah K. Schröder-Lange, Ralf Weiskirchen

“…Additionally, we analyzed the transcriptome of Rat-1 cells through mRNA sequencing (mRNA-Seq) using next-generation sequencing (NGS). Finally, typical features of these fibroblastic cells were determined using electron microscopy, Western blotting, and fluorescent phalloidin conjugates.…”
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Case report: A panorama gene profile of ovarian cancer metastasized to axillary lymph node by Yu Xia, Yu Huang, Zheng Liu, Siyuan Song, Yi Wang, Yi Wang, Jing Luo

“…To unravel the carcinogenesis, the next-generation sequencing (NGS) was performed.ResultsConventional examinations and imaging suggested the presence of both occult breast cancer and ovarian cancer. …”
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Autosomal Recessive Congenital Sensorineural Hearing Loss due to a Novel Compound Heterozygous PTPRQ Mutation in a Chinese Family by Xia Wu, Shan Wang, Sen Chen, Ying-ying Wen, Bo Liu, Wen Xie, Dan Li, Lin Liu, Xiang Huang, Yu Sun, Wei-jia Kong

“…Its mutations can cause the defects of stereocilia in hair cell, which lead to nonsyndromic sensorineural hearing loss. Using next-generation sequencing and Sanger sequencing method, we identified a novel compound heterozygous missense mutation, c.4472C>T p.T1491M (maternal allele) and c.1973T>C p.V658A (paternal allele), in PTPRQ gene. …”
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Unveiling the rhizosphere microbiome of Dendrobium: mechanisms, microbial interactions, and implications for sustainable agriculture by Surendra Sarsaiya, Surendra Sarsaiya, Archana Jain, Ranjan Singh, Qihai Gong, Qin Wu, Jishuang Chen, Jingshan Shi, Jingshan Shi

“…Additionally, it addresses modern techniques for analyzing microbial communities, including metagenomics and next-generation sequencing, and their applications in advancing precision agriculture. …”
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Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion by Wen-jing Meng, Zi-lan Wen, Risto Kasanen, Hui Sun, Hui Sun, Fred O. Asiegbu

“…In this study, using next-generation sequencing technology, we investigated the microbial community in different tree regions (needles, upper stem, and lower stem) of Norway spruce with and without wood decay symptoms. …”
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Differential Regulation of NK Cell Receptors in Acute Lymphoblastic Leukemia by Le Jie Lee, Norfarazieda Hassan, Siti Zuleha Idris, Suresh Kumar Subbiah, Heng Fong Seow, Norhafizah Mohtaruddin, Kian Meng Chang, Raudhawati Osman, Hishamshah Mohd Ibrahim, Sheila Nathan, Maha Abdullah

“…Influence of ALL on NK cell is still unclear. Targeted next-generation sequencing was conducted on 62 activating/inhibitory receptors, ligands, effector, and exhaustion molecules on T-ALL (6 males) and normal controls (NC) (4 males and 4 females). …”
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Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders by Sarisa Klinhom, Chanon Kunasol, Sirawit Sriwichaiin, Sasiwan Kerdphoo, Nipon Chattipakorn, Siriporn C. Chattipakorn, Chatchote Thitaram

“…The samples were subjected to DNA extraction and amplification targeting the V3-V4 region of 16S rRNA gene for next-generation sequencing analysis. Elephants with GI symptoms exhibited a decreased microbial stability, as characterized by a significant reduction in microbiota diversity within individual guts and notable differences in microbial community composition when compared with healthy elephants. …”
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An X-Linked Hyper-IgM Patient Followed Successfully for 23 Years without Hematopoietic Stem Cell Transplantation by Necil Kutukculer, Neslihan Edeer Karaca, Guzide Aksu, Ayca Aykut, Erhan Pariltay, Ozgur Cogulu

“…In 2017, targeted next generation sequencing was performed in order to understand his molecular pathology. …”
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A study of macroinvertebrate communities in Bolshiye Koty Bay of Lake Baikal using DNA metabarcoding by L. S. Kravtsova, T. E. Peretolchina, T. I. Triboy, I. A. Nebesnykh, A. E. Tupikin, M. R. Kabilov

“…The approach proposed for studying the structure of macroinvertebrate communities based on DNA metabarcoding and next generation sequencing can be recommended for express assessment of the state of aquatic ecosystems in the monitoring.…”
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Recombinant AAV batch profiling by nanopore sequencing elucidates product-related DNA impurities and vector genome length distribution by Florian Dunker-Seidler, Kathrin Breunig, Magdalena Haubner, Florian Sonntag, Markus Hörer, Rebecca C. Feiner

“…Comprehensive analysis of encapsidated DNA by long-read next-generation sequencing is destined to guide platform optimization and provide crucial insights into safety of gene therapies. …”
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Pathogenicity of avian reovirus variant in the immune organs of broiler chicks by Haiyang Yu, Yudong Zhu, Qiong Wu, Wanjun Zhao, You Wang, Dong Wang, Huaguang Lu, Youxiang Diao, Yijing Li, Yi Tang

“…In this study, an ARV strain (Chicken/XJ/China/2022/01/16) was isolated from arthritic broiler chicks in Xinjiang province and whole genome sequencing of the ARV strain was performed using next generation sequencing (NGS) technology, and sequence analysis showed that the strain was a type V variant of the ARV gene. …”
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Chromothripsis in Treatment Resistance in Multiple Myeloma by Kyoung Joo Lee, Ki Hong Lee, Kyong-Ah Yoon, Ji Yeon Sohn, Eunyoung Lee, Hyewon Lee, Hyeon-Seok Eom, Sun-Young Kong

“…First, we analyzed nine MM cell lines (U266, MM.1S, RPMI8226, KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, KMS-28-PE, and NCI-H929) and bone marrow samples of four patients who were diagnosed with MM by next-generation sequencing-based copy number variation analysis. …”
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