Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model by Chun-Yi Wu, Hsin-Hua Hsieh, Pei-An Chu, Wen-Hsiang Hong, Ting-Yu Chang, Chia-Fang Hsu, Siao-Ting Lin, Po-Hsun Su, Shin-Lei Peng

“…Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (n=3), 1 (n=3), 2 (n=3), and 3 (n=3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. …”
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Antimicrobial Susceptibility Profiles of Commensal <i>Staphylococcus</i> spp. Isolates from Chickens in Hungarian Poultry Farms Between 2022 and 2023 by Ábel Szabó, Ákos Jerzsele, László Kovács, Ádám Kerek

“…To uncover the underlying causes of multidrug resistance, next-generation sequencing can be employed to elucidate the genetic basis of phenotypic resistance.…”
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Exogenous Janus Kinase 617 Codon Influences Small Noncoding RNAs and Gene Expression in Ba/F3 Cells by Yi-Yang Chen, Ying-Hsuan Wang, Chih-Cheng Chen, Cih-En Huang, Chia-Chen Hsu, Shu-Huei Hsiao, Yu-Wei Leu

“…Human wild type versus V617F-mutated JAK2 are expressed in mouse Ba/F3 cells, and the expressed small and total RNAs were subjected to next generation sequencing analysis to determine the direct induction. …”
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Leveraging mRNA technology for antigen based immuno-oncology therapies by Wei Zheng, Michele Ceccarelli, Charalampos S Floudas, Siranush Sarkizova

“…The identification and prioritization of patient-specific tumor neoantigens are based on advanced computational algorithms taking advantage of the profiling with next-generation sequencing considering factors involved in human leukocyte antigen (HLA)-peptide-T-cell receptor (TCR) complex formation, including peptide presentation, HLA-peptide affinity, and TCR recognition. …”
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Fast and accurate imputation of genotypes from noisy low-coverage sequencing data in bi-parental populations. by Cécile Triay, Alice Boizet, Christopher Fragoso, Anestis Gkanogiannis, Jean-François Rami, Mathias Lorieux

“…<h4>Motivation</h4>Genotyping of bi-parental populations can be performed with low-coverage next-generation sequencing (LC-NGS). This allows the creation of highly saturated genetic maps at reasonable cost, precisely localized recombination breakpoints (i.e., the crossovers), and minimized mapping intervals for quantitative-trait locus analysis. …”
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Characteristics of Lung Microbiota in Children’s Refractory Mycoplasma pneumoniae Pneumonia Coinfected with Human Adenovirus B by Wenxiang Zhou, Jinglong Chen, Zhimin Xi, Yanyan Shi, Libo Wang, Aizhen Lu

“…The characteristics of the clinical findings were examined, and the lung microbiota was analyzed by metagenomic next generation sequencing (mNGS). Results. Eleven patients in the MP&ADV group were coinfected with human mastadenovirus species B. …”
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A patient with long-term diabetes dies following infection with Francisella novicida in Guangdong province, China: a case report by Linjin Fan, Jialong Guan, Lizhi Feng, Yulong Wang, Huimin Zeng, Ying Zhu, Hao Li, Qiang Chen, Linghua Li, Jun Qian, Linna Liu, Yueping Li, Wei Zhao

“…Microbiological confirmation of F. novicida infection relies on metagenomic next-generation sequencing (mNGS) and pdpD-2 gene-specific identification. …”
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Improved ChIP Sequencing for H3K27ac Profiling and Super-Enhancer Analysis Assisted by Fluorescence-Activated Sorting of Formalin-Fixed Paraffin-Embedded Tissues by Nenggang Jiang, Zhihao Wen, Huan Tao, Hongyan Liao

“…Although protocol of chromatin immunoprecipitation coupled with next generation sequencing (NGS) (ChIP-seq) using FFPE samples has been established, removal of interference signals from non-target cell components in the samples is still needed. …”
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An Egyptian child with Wolman disease presenting with hemophagocytic lymphohistiocytosis by Rabab El Hawary, Safa Meshaal, Alia S. Eldash, Sohilla Lotfy, Dalia Abd Elaziz, Radwa Alkady, Rania Darwish, Aya Erfan, Mai Saad, Engy Chohayeb, Nermeen Galal, Aisha M. Elmarsafy

“…The patient had elevated levels of ferritin, triglycerides, fibrinogen, and soluble CD25, and thus, the diagnosis of HLH was suspected. Next-generation sequencing was performed, and it revealed one homozygous previously reported pathogenic variant in LIPA gene: c.398del; p. …”
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Optimizing the NGS-based discrimination of multiple lung cancers from the perspective of evolution by Ziyang Wang, Xiaoqiu Yuan, Kunkun Sun, Fang Wu, Ke Liu, Yiruo Jin, Olga Chervova, Yuntao Nie, Airong Yang, Yichen Jin, Jing Li, Yun Li, Fan Yang, Jun Wang, Stephan Beck, David Carbone, Guanchao Jiang, Kezhong Chen

“…Abstract Next-generation sequencing (NGS) offers a promising approach for differentiating multiple primary lung cancers (MPLC) from intrapulmonary metastasis (IPM), though panel selection and clonal interpretation remain challenging. …”
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Sox9-Increased miR-322-5p Facilitates BMP2-Induced Chondrogenic Differentiation by Targeting Smad7 in Mesenchymal Stem Cells by Yongsheng Zeng, Chengcheng Du, Pengcheng Xiao, Yiting Lei, Piao Zhao, Zhenglin Zhu, Shengqiang Gao, Bowen Chen, Shengwen Cheng, Wei Huang, Chen Zhao

“…We found that miR-322-5p meets the requirement through next-generation sequencing (NGS) and bioinformatic analysis. …”
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Characterization of the Merkel Cell Carcinoma miRNome by Matthew S. Ning, Annette S. Kim, Nripesh Prasad, Shawn E. Levy, Huiqiu Zhang, Thomas Andl

“…To identify microRNAs specific to MCC (MCC-miRs), next-generation sequencing (NGS) of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin. …”
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Mutational Analysis of a Familial Adenomatous Polyposis Pedigree with Bile Duct Polyp Phenotype by Li-jun Xie, Dan-dan Ruan, Jian-hui Zhang, Yi Li, Li Chen, Mao-lin Yan, Ming-dian Yu, Jie-wei Luo, Hui-zhen Zhang

“…Different mutation sites in APC are associated with the severity of FAP, risks of carcinogenesis, and extraintestinal manifestations. We used next-generation sequencing (NGS) and capture techniques to screen suspected mutation points in the proband in this pedigree. …”
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The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels by Andrey A. Ayginin, Ekaterina V. Pimkina, Alina D. Matsvay, Anna S. Speranskaya, Marina V. Safonova, Ekaterina A. Blinova, Ilya V. Artyushin, Vladimir G. Dedkov, German A. Shipulin, Kamil Khafizov

“…Advances in the next generation sequencing (NGS) technologies have significantly increased our ability to detect new viral pathogens and systematically determine the spectrum of viruses prevalent in various biological samples. …”
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X-Linked Autism Type 9 Caused by a Hemizygote Pathogenic Variant in the TMLHE Gene: Etiological Diagnosis in an Adult Male with Moderate Intellectual Disability by Verhoeven WM, Pfundt R, Engelke UF, Kluijtmans LA, Egger JI

“…To prevent regressive autistic episodes in young children, it is highly recommended to consider next-generation sequencing techniques as the first step in the differential diagnostic process of autism.Keywords: epsilon-trimethyllysine hydroxylase gene, TMLHE, levocarnitine, autism, intellectual disability, contextual neuropsychology, psychopathology…”
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Discovery of a novel Mediterranean Haemaphysalis (Ornithophysalis) doenitzi group tick species infesting Falco eleonorae on Antikythira Island, Greece by Lidia Chitimia-Dobler, Christos Barboutis, Anastasios Bounas, Christina Kassara, Ben J. Mans, Anastasios Saratsis, Ala Tabor

“…Following comprehensive morphological assessment and genetic analysis of the mitochondrial genome by means of next-generation sequencing of both adult and nymphal stages of the ticks, our empirical findings substantiate the delineation of a previously unclassified species. …”
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Mitochondrial genome insights into the phylogenetics and biogeographic evolution of snow trout (Cyprinidae, Schizothorax) in the Tien Shan Mountains by Akbarjon Rozimov, Yufan Wang, Min Wang, Ming Zou, Jobir Sobirov, Erkin Karimov, Bakhtiyor Kholmatov, Jörg Freyhof, Sirojiddin Namozov, Chongnv Wang, Xinxin Li, Baocheng Guo

“…Here, we analyzed three mitochondrial genomes assembled using the next-generation sequencing data of three snow trout species, S. eurystomus, S. fedtschenkoi, and Schizothorax sp., from the western Tien Shan Mountains. …”
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Invasive Klebsiella pneumoniae liver abscess syndrome complicated by carbapenem-resistant Acinetobacter baumannii infection: a case report by Qiupeng Feng, Qiupeng Feng, Hua Yuan, Hua Yuan, Jin Ma, Jin Ma, Zhiqiang Guo, Zhiqiang Guo, Xiaohua Xia, Xiaohua Xia, Guang Zhao, Guang Zhao

“…The patient underwent puncture and drainage, with analysis of the drainage fluid, sputum culture, and metagenomic next-generation sequencing (m-NGS) revealing a co-infection with blood-borne Klebsiella pneumoniae and Acinetobacter baumannii. …”
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Mutational Analysis of Extranodal NK/T-Cell Lymphoma Using Targeted Sequencing with a Comprehensive Cancer Panel by Seungkyu Choi, Jai Hyang Go, Eun Kyung Kim, Hojung Lee, Won Mi Lee, Chun-Sung Cho, Kyudong Han

“…The recent technological advancements in next-generation sequencing (NGS) have made DNA sequencing cost- and time-effective, with more reliable results. …”
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Pleura-Based Lipomatous Neoplasm with RUNX1T1::PLAG1 Rearrangement and RB1 Gene Deletion by Rayan Sibira, Anna Vu, Ryan Martinez, Emilian Racila, Siddhartha Sen, Diana Oramas

“…Remarkably, the next-generation sequencing assay unveiled a previously unreported RUNX1T1::PLAG1 fusion, in addition to RB1 gene deletion. …”
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