stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing. by Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z Metcalfe

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Next generation sequencing of multiple SARS-CoV-2 infections in the Omicron Era by Marzia Cavalli, Giulia Campoli, Anna Anselmo, Rossella Brandi, Antonella Fortunato, Maria Di Spirito, Anella Monte, Martina Lipari, Manfredo Bortone, Vanessa Vera Fain, Raffaele D’Amelio, Florigio Lista, Silvia Fillo

“…This analysis involved Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR) and Next Generation Sequencing (NGS). RT-qPCR was performed on 416,466 naso-oropharyngeal swabs, with 10,380 samples further analyzed using NGS technology. …”
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Detection of human noroviruses in sewage by next generation sequencing in Shandong Province, 2019–2021 by Suting Wang, Mingyi Xu, Xiaojuan Lin, Ping Xiong, Yao Liu, Aiqiang Xu, Meng Chen, Shengxiang Ji, Zexin Tao

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Identification of novel genetic variants associated with feline cardiomyopathy using targeted next-generation sequencing by Jade Raffle, Jose Novo Matos, Marsha Wallace, Lois Wilkie, Richard J. Piercy, Perry Elliott, David J. Connolly, Virginia Luis Fuentes, Androniki Psifidi

“…DNA was extracted from residual blood, and targeted next-generation sequencing was performed on two separate feline cohorts: an across-breed cohort (23 healthy cats and 21 HCM-affected pedigree or Domestic Shorthair cats), and a within-breed cohort of Birman pedigree cats (14 healthy, 8 HCM-affected, and 6 RCM-affected). …”
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Next-generation sequencing (NGS) for the personalized treatment of head and neck squamous cell carcinoma (HNSCC) by Prasad Monisha, Ramadurai Murugan

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Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China by Rui Zhao, Guilun He, Lin Xiang, Melinda Ji, Rongheng He, Xudong Wei

Subjects: “…metagenomic next-generation sequencing assists…”
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Pituitary Metastatic Composite Tumors: A Case Report with Next-Generation Sequencing and Review of the Literature by Matthew Helton, Muhammad Abu-Rmaileh, Kevin Thomas, Murat Gokden, Alissa Kanaan, Analiz Rodriguez

“…In our patient, the presence of VHL and Notch2 mutations in both tumors highlights the possibility of using next-generation sequencing to help identify therapeutic targets even in complex composite neoplasms.…”
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Genetic Etiology of Ichthyosis in Turkish Patients: Next-generation Sequencing Identified Seven Novel Mutations by Hanife SAAT, Ibrahim SAHIN, Neslihan DUZKALE, Muzeyyen GONUL, Taha BAHSI

“…The clinical use of next-generation sequencing (NGS) methodologies is beneficial in the diagnostic approach to ichthyosis and genetic counseling. …”
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Combination of metagenomic next-generation sequencing and morphology for identifying Coccidioides immitis: a case report by Hong Liu, Kaixin Zhou, Chaoran Zhong, Ming Guan

Subjects: “…metagenomic next-generation sequencing (mNGS)…”
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Targeted Next-Generation Sequencing in Pneumonia: Applications in the Detection of Responsible Pathogens, Antimicrobial Resistance, and Virulence by Liu B, Bao Z, Chen W, Xi X, Ge X, Zhou J, Zheng X, Zhang P, Deng W, Ding R, Zhou M, Fang J

Subjects: “…targeted next-generation sequencing…”
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Deployment of next-generation sequencing approach for variant detection in myocardial infarction: A concise investigation by Desaraju Suresh Bhargav, Nadeem Siddiqui, Bhadra Murthy V, Vijaya Tartte, Vanaja V

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Application of Targeted Next-Generation Sequencing in Bronchoalveolar Lavage Fluid for the Detection of Pathogens in Pulmonary Infections by Dai X, Xu K, Tong Y, Li J, Dai L, Shi J, Xie H, Chen X

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A Rare Case of Cladophialophora bantiana Intracranial Infection: Highlighting the Utility of Next-Generation Sequencing in Diagnosis by Melissa Whitman, Nicole Vissichelli

“…Here, we report a rare case of cerebral ventriculitis with development of a cerebral abscess due to C. bantiana diagnosed by cell-free DNA next-generation sequencing. Noninvasive diagnostics led to earlier disease identification and initiation of antifungal therapy, which has the potential to reduce mortality in this highly fatal disease.…”
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PIK3CA and p53 Mutations by Next Generation Sequencing in Lymphoepithelioma-Like Carcinoma of the Endometrium by Lucie Bienfait, Nicky D’Haene, Xavier Catteau, Jean-Christophe Noël

“…In particular, using the next generation sequencing (NGS) technique, we have demonstrated that this tumor could be associated with PIK3CA and p53 gene mutations. …”
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Risk Factors and Outcome of Sepsis in Traumatic Patients and Pathogen Detection Using Metagenomic Next-Generation Sequencing by Yiqing Tong, Jianming Zhang, Yimu Fu, Xingxing He, Qiming Feng

“…In this study, we aimed to analyze the risk factors and outcome of sepsis in traumatic patients and to investigate the performance of metagenomic next-generation sequencing (mNGS) compared with standard microbiological diagnostics in post-traumatic sepsis. …”
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Impact of Herpesvirus Detection via Metagenomic Next-Generation Sequencing in Patients with Lower Respiratory Tract Infections by Zhang H, Zhang X, Zheng Y, Gu X, Fu Z, Gai W, Wang H

“…., Ltd, No. 156 Jinghai 4th Road, Beijing Economic and Technological Development Zone, Beijing, 101103, People’s Republic of China, Email weigai@willingmed.com Huaying Wang, The Affiliated People’s Hospital of Ningbo University, Yinzhou People’s Hospital, No. 251, Baizhang East Road, Ningbo, 315040, People’s Republic of China, Email yingmeire@163.comPurpose: This study aimed to investigate the impact of herpesvirus detection by metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF) on lower respiratory tract infections (LRTIs) patients’ lung microbiome composition and prognosis.Patients and methods: We initially enrolled 234 hospitalized patients with LRTIs who underwent BALF mNGS between February 2022 and May 2023. …”
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Developing transcriptomic biomarkers for TAVO412 utilizing next generation sequencing analyses of preclinical tumor models by Ying Jin, Peng Chen, Huajun Zhou, Guangmao Mu, Simin Wu, Zhengxia Zha, Bin Ma, Chao Han, Mark L. Chiu, Mark L. Chiu

“…ConclusionsIn implementing precision medicine by leveraging preclinical model data, a predictive transcriptomic biomarker empowered by next-generation sequencing was identified that could optimize the selection of patients that may benefit most from TAVO412 treatment. …”
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Clinicopathological and molecular analyses of uterine carcinosarcomas using next-generation sequencing: A single-center experience by Ezgi Genç Erdoğan, Tülin D. Yalta, Nuray Can, Necdet Süt, Ebru Taştekin, Ufuk Usta, Fulya Öz Puyan, Fatma E. Usturalı Keskin, Busem B. Kurt

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Clinical Utility of Combined Tissue and Plasma Next-Generation Sequencing in Patients With Advanced, Treatment-Naïve NSCLC by Helena Bote-de Cabo, MD, Marco Siringo, MD, Esther Conde, MD, PhD, Susana Hernández, PhD, Fernando López-Ríos, MD, PhD, Alicia Castelo-Loureiro, MD, Esther García-Lorenzo, MD, Javier Baena, MD-PhD, Mercedes Herrera, MD, Ana Belén Enguita, MD, Yolanda Ruano, PhD, Jon Zugazagoitia, MD, PhD, Luis Paz-Ares, MD, PhD

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Inferring Tripartite Associations of Vector-Borne Plant Pathogens Using a Next-Generation Sequencing Approach by Ava M. Gabrys, Christopher H. Dietrich, Valeria Trivellone

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