Intermediate between Idiopathic Hypereosinophilia and Chronic Eosinophilic Leukemia: A Report of Two Hypereosinophilic Cases with Possible Novel Molecular Mutations by Jui Choudhuri, Mohammad Eskandari, Yang Shi, Yanhua Wang

“…Here, we report two cases of hypereosinophilia in which five possible novel molecular mutations were identified by targeted next-generation sequencing (NGS) analysis. They were FBXW7, KM2A, TCF3, ERBB4, and MET. …”
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Impact of Extracellular Matrix-Related Genes on the Tumor Microenvironment and Prognostic Indicators in Esophageal Cancer: A Comprehensive Analytical Study by Yinghong Wu, Wenjie Hu, Zhihong Jia, Qiying Zhu, Jinghui Xu, Liang Peng, Renjie Wang

“…Our study utilized next-generation sequencing and clinical data from esophageal cancer patients provided by The Cancer Genome Atlas, employing the R package in RStudio for computational analysis. …”
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Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps by Jiachao Xiong, Zhixiao Liu, Minliang Wu, Mengyan Sun, Yu Xia, Yuchong Wang

“…We analyzed the differential microRNA (miRNA) expression in ADSC-Exo using next-generation sequencing (NGS) technology to explore their potential mechanisms in promoting vascularization. …”
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Orientia, Rickettsia, and the microbiome in rodent attached chiggers in North Carolina, USA. by Elise A Richardson, Reuben Garshong, Kaiying Chen, Dac Crossley, Bryan S Mclean, Gideon Wasserberg, Charles S Apperson, R Michael Roe, Loganathan Ponnusamy

“…DNA was extracted from the chiggers, and the V3-V4 regions of the bacterial 16S rRNA gene were sequenced using next-generation sequencing (NGS). Alpha diversity metrics revealed significant differences in bacterial diversity among different collection counties. …”
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Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…Traditional experimental methods involve genomic profiling techniques such as next­generation sequencing and fluorescence in situ hybridization. …”
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High-throughput sequence-based microsatellite genotyping for the non-model Neotropical tree species Anadenanthera colubrina (Leguminosae) by Alejandra Lorena Goncalves, María Victoria García, Emilie Chancerel, Olivier Lepais, Myriam Heuertz

“…Previously available genetic markers for A. colubrina consisted of a few microsatellites. Next-generation sequencing (NGS) strategies allow simple and cost-effective development of new SSR loci from low-coverage whole genome shotgun sequencing. …”
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Identification of a Novel Non-V600E BRAF Mutation in Papillary Thyroid Cancer by Marco Capezzone, Maja Rossi, Elisabetta Macerola, Silvia Cantara, Francesco Pepe, Eugenia Maria Morabito, Gilda Dalmazio, Sara Bardi, Agostino Ognibene, Massimo Alessandri, Gabriele Materazzi, Luigi De Napoli, Michele Cirianni, Liborio Torregrossa

“…We report a novel non-V600E mutation (c.1799_1812delinsAT, p.V600_W604delinsD), identified preoperatively with next-generation sequencing (NGS) on the material obtained with fine-needle aspiration cytology (FNAC) performed on a thyroid nodule cytologically suspicious for malignancy in a 35-year-old male patient. …”
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NLRP3 Is Expressed in the Spiral Ganglion Neurons and Associated with Both Syndromic and Nonsyndromic Sensorineural Deafness by Penghui Chen, Longxia He, Xiuhong Pang, Xiaowen Wang, Tao Yang, Hao Wu

“…Though a variety of targeted next-generation sequencing (NGS) panels has been recently developed to facilitate genetic screening of nonsyndromic deafness, some syndromic deafness genes outside the panels may lead to clinical phenotypes similar to nonsyndromic deafness. …”
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Mitochondrial DNA Structure in <i>Trypanosoma cruzi</i> by Alfonso Herreros-Cabello, Francisco Callejas-Hernández, Manuel Fresno, Núria Gironès

“…They consist of coding and divergent/variable regions, complicating their assembly due to repetitive elements. However, next-generation sequencing (NGS) methods have resolved these issues, enabling the complete sequencing of maxicircles from different strains. …”
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The First Infant Bloodstream Infection Caused by Pantoea dispersa in China: A Case Report and Literature Review by Yu W, Sun Z, Wang M, Li Z, Zhang C, Sun Y, Wang S

“…Through MALDI Biotyper and next-generation sequencing techniques, the pathogen was identified as P. dispersa. …”
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Phenotypic and molecular characterization of the largest worldwide cluster of hereditary angioedema type 1. by Juan Sebastian Arias-Flórez, Sandra Ximena Ramirez, Bibiana Bayona-Gomez, Lina Castro-Castillo, Valeria Correa-Martinez, Yasmín Sanchez-Gomez, William Usaquén-Martínez, Lilian Andrea Casas-Vargas, Carlos Eduardo Olmos Olmos, Nora Contreras Bravo, Camilo Andres Velandia-Piedrahita, Adrien Morel, Rodrigo Cabrera-Perez, Natalia Santiago-Tovar, Cristian Camilo Gaviria-Sabogal, Ingrid Tatyana Bernal, Dora Janeth Fonseca-Mendoza, Carlos M Restrepo

“…A geographically HAE1 cluster was found in the northeast Colombian department of Boyaca, which accounts for four unrelated families, with 79 suspected to be affected members. Next-Generation Sequencing (NGS) was performed in 2 out of 4 families (Family 1 and Family 4), identifying the variants c.1420C>T and c.1238T>G, respectively. …”
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High-Risk Genotypes Associated with Poor Response to Controlled Ovarian Stimulation in Indian Women by Anuradha Udumudi, K. Chaitra Lava, Vandana Hegde

“…Materials and Methods: A total of 312 females were included in the study comprising 212 women with infertility and 100 controls. Next-generation sequencing technology was employed to sequence multiple genes associated with response to COS. …”
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Chlamydia psittaci pneumonia - evolutionary aspects on chest CT by Na Yang, Zhengqiu Ou, Qian Sun, Junping Pan, Jing Wu, Chen Xue

“…Methods In total, 80 cases of C. psittaci pneumonia diagnosed through next-generation sequencing from January 2019 to December 2023 in multiple hospitals in China were collected according to the inclusion criteria and analyzed. …”
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Wilson Disease in a Turkish Population: Molecular Insights into an Old Disease with Reported and Novel Variants by Abdullatif Bakır, Vehap Topçu, Büşranur Çavdarlı

“…The molecular genetic diagnosis was performed using the next-generation sequencing method. Results: Molecular genetic analysis revealed 26 different variants, two of which were novel c.1707+4A>G (IVS4+4A>G) and p.M497K in 34 patients from 31 different families. p.M769Hfs*26 was the variant with the highest allele frequency at 11.3%, followed by the p.H1069Q variant (8%). …”
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Genetic Diversity of Human Enterovirus in Kazakhstan, during 2022 by Dinara Kamalova, Assel Akhmetova, Asylulan Amirgazin, Igor Sytnik, Viktoriya Rudenko, Gulzhan Yessimkhanova, Dinagul Bayesheva, Sergey Yegorov, Alexander Shevtsov

“…We then genotyped enteroviral subtypes associated with AM (n = 50) and HA (n = 9) using next-generation sequencing (NGS) on the viral protein 1 (VP1), followed up by whole-genome sequencing of the isolated viral species. …”
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Sagittal Craniosynostosis with Uncommon Anatomical Pathologies in a 56-Year-Old Male Cadaver by Andrey Frolov, Craig Lawson, Joshua Olatunde, James T. Goodrich, John R. Martin III

“…Exome sequencing of DNA extracted from the cadaveric tissue specimen performed on the Next Generation Sequencing (NGS) platform yielded 81 genetic variants identified as pathologic. …”
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Sanger validation of WGS variants by Arina Kopernik, Mariia Sayganova, Gaukhar Zobkova, Natalia Doroschuk, Anna Smirnova, Daria Molodtsova-Zolotukhina, Olesya Sagaydak, Oxana Ryzhkova, Sergey Kutsev, Olga Groznova, Lyusya Melikyan, Elizaveta Bondarchuk, Mary Woroncow, Eugene Albert, Viktor Bogdanov, Pavel Volchkov

“…Abstract With the development of next-generation sequencing (NGS) technologies it became possible to simultaneously analyze millions of variants. …”
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Challenges in Medicine: The Odyssey of a Patient with Isolated IgG4-Related Eosinophilic Angiocentric Fibrosis Presenting as a Locally Destructive Sinonasal Mass by Snigdha Nutalapati, Richard O’Neal, William O’Connor, Brett T. Comer, Gerhard C. Hildebrandt

“…Our case interestingly also had T-cell clonality and isolated isocitrate dehydrogenase 2 enzyme mutation on next-generation sequencing, suggesting a possible role of novel molecular-targeted therapies in this rare disease. …”
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Development and validation of a portable device for lab-free versatile nucleic acid extraction by Anthony J. Politza, Tianyi Liu, Aneesh Kshirsagar, Ming Dong, Md. Ahasan Ahamed, Weihua Guan

“…By providing an effective on-site sample preparation solution, the widespread adoption of low centrifugal extractions will improve the sensitivity and reliability of NAT and will positively impact other point-of-care technologies such as next generation sequencing (NGS), biomarker detection, and environmental monitoring.…”
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The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution by Meichen Pan, Sarah O’Flaherty, Ashley Hibberd, Svetlana Gerdes, Wesley Morovic, Rodolphe Barrangou

“…Abstract Background The advent of next generation sequencing technologies has enabled a surge in the number of whole genome sequences in public databases, and our understanding of the composition and evolution of bacterial genomes. …”
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