Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer by Jeremy L. Pinyon, Georg vonJonquieres, Stephen L. Mow, Amr Al Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H. Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley

“…Abstract Viral vector and lipid nanoparticle based gene delivery have limitations around spatiotemporal control, transgene packaging size, and vector immune reactivity, compromising translation of nucleic acid (NA) therapeutics. …”
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Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1 by Xinxin Li, Jinghua Cui, Zanbo Ding, Ziyan Tian, Yiming Kong, Linghai Li, Yang Liu, Wen Zhao, Xueying Chen, Han Guo, Zhengshuo Cui, Xinwei Li, Jing Yuan, Huina Zhang

“…Methods K.pn EVs were purified from the bacterial suspension using ultracentrifugation and characterized by transmission electron microscopy nanoparticle tracking analysis, and EV marker expression. …”
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mRNA-LNP vaccines combined with tPA signal sequence elicit strong protective immunity against Klebsiella pneumoniae by Ting Huang, Siyou Che, Zheng Lv, Danrui Hao, Runyu Wang, Qinxuan Yi, Ling Mei, Yang Yuan, Hang Zou, Yidong Guo, Xinrong Wang, Yiwen Chu, Kelei Zhao

“…The results showed that lipid nanoparticle (LNP)-YidR-mRNA vaccine was produced with good morphology, high the encapsulation efficiency, and the specific antigen was highly expressed in cells in vitro. …”
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Small Extracellular Vesicles from Young Healthy Human Plasma Inhibit Cardiac Fibrosis After Myocardial Infarction via miR-664a-3p Targeting SMAD4 by Wang W, Li Y, Zhang C, Zhou H, Li C, Cheng R, Chen X, Pu Y, Chen Y

“…Weiwei Wang,1,* Ying Li,1,* Cheng Zhang,2,* Haoyang Zhou,1 Chunyu Li,3 Rong Cheng,1 Xufeng Chen,1 Yanan Pu,4 Yan Chen1,5,6 1Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People’s Republic of China; 2Long Jiang Central Laboratory, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, People’s Republic of China; 3Long Jiang Intensive Care Unit, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People’s Republic of China; 4Department of Clinical Laboratory, Nanjing Chest Hospital, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, 210029, People’s Republic of China; 5Department of Emergency and Critical Care Medicine, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, 215000, People’s Republic of China; 6Department of Emergency Management, School of Health Policy & Management, Nanjing Medical University, Nanjing, 211166, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Chen; Yanan Pu, Email chenyandoc@njmu.edu.cn; pyn342626@njmu.edu.cnPurpose: Cardiac fibrosis, a key contributor to ventricular pathologic remodeling and heart failure, currently lacks effective therapeutic approaches.Patients and Methods: Small extracellular vesicles from young healthy human plasma (Young-sEVs) were characterized via protein marker, transmission electron microscopy, and nanoparticle tracking analysis, then applied in cellular models and mouse models of cardiac fibrosis. …”
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Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo by Claudia Palazzo, Roberta Mastrantonio, Noemi Gioelli, Erika Testa, Francesco Recco, Donatella Lucchetti, Giulia Villari, Alessio D’Alessio, Alessandro Sgambato, Flavio Mignone, Guido Serini, Maria Teresa Viscomi, Luca Tamagnone

“…The quality of exosome-enriched preparations was validated by the presence of specific markers in western blotting, as well as by light scattering and nanoparticle tracking analysis. Wound healing, transwell, and digital real-time migration assays were carried out to assess the activity of cancer cell-derived exosomes in the regulation of endothelial cells. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Methods: Immunophenotype and secretory ability at soluble protein and EV-related levels, including embedded miRNAs, were analysed in the secretomes by means of flow cytometry, nanoparticle tracking analysis, high throughput ELISA and qRT-PCR arrays. …”
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Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy by Wenjing Bai, Jinxin Xu, Wenbin Gu, Danyang Wang, Ying Cui, Weidong Rong, Xiaoan Du, Xiaoxia Li, Cuicui Xia, Qingqing Gan, Guantao He, Huahui Guo, Jinfeng Deng, Yuqiong Wu, Ray-Whay Chiu Yen, Srinivasan Yegnasubramanian, Scott B. Rothbart, Cheng Luo, Linping Wu, Jinsong Liu, Stephen B. Baylin, Xiangqian Kong

“…For translating these findings to cancer therapy, we use a lipid nanoparticle (LNP)-mediated mRNA delivery approach in which the short mSTELLA, but not hSTELLA regions are required to reverse cancer-specific DNA hypermethylation and impair colorectal cancer tumorigenicity.…”
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Performance evaluation of four cascade impactors for airborne ultrafine-particle (UFP) collection: the influence of particle type, concentration, mass, and chemical nature by E. Eckenberger, A. Mittereder, N. Gawlitta, N. Gawlitta, J. Schnelle-Kreis, J. Schnelle-Kreis, M. Sklorz, D. Brüggemann, R. Zimmermann, R. Zimmermann, A. C. Nölscher

“…Therefore, we conducted a comprehensive characterization and comparison of four cascade impactors suitable for separating and collecting UFPs – namely, the 120R Micro-Orifice Uniform Deposit Impactor (120R MOUDI-II), ultraMOUDI, electrical low-pressure impactor (ELPI), and personal nanoparticle sampler (PENS) – under controlled laboratory conditions and in a field application.…”
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Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application by Ya-chen Hou, Jing-an Li, Chang Cao, Chang Su, Zhen Qin, Ge Zhang, Jia-cheng Guo, Jun-nan Tang, Jin-ying Zhang, Shao-kang Guan

“…In this work, the biodegradable Mg-Zn-Y-Nd alloy was modified by MgF2 and dopamine polymer film (PDA) as the corrosion resistance layer and the bonding layer respectively, and then the exosome, a natural nanoparticle contains mRNAs and proteins, was tailored to give the surface better biocompatibility. …”
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Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties by Jan Šťastný, Ángela Morellá-Aucejo, Ángela Morellá-Aucejo, Ángela Morellá-Aucejo, Tomáš Skala, Andrea Bernardos, Andrea Bernardos, Andrea Bernardos, Petr Maršík, Araceli Lérida-Viso, Araceli Lérida-Viso, Araceli Lérida-Viso, Jaroslav Matějka, Anna Mascellani Bergo, María Dolores Marcos, María Dolores Marcos, María Dolores Marcos, Ramón Martínez-Máñez, Ramón Martínez-Máñez, Ramón Martínez-Máñez, Ivan Jablonský, Pavel Klouček

“…This study is an essential prerequisite for further steps like nanoparticle functionalization for sustained or on-command delivery of these natural extracts.…”
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Multimodal lung cancer theranostics via manganese phosphate/quercetin particle by Chong Qiu, Fei Xia, Qingchao Tu, Huan Tang, Yinan Liu, Hongda Liu, Chen Wang, HaiLu Yao, Linying Zhong, Yuanfeng Fu, Pengbo Guo, Weiqi Chen, Xinyu Zhou, Li Zou, Licheng Gan, Jiawei Yan, Yichong Hou, Junzhe Zhang, Huanhuan Pang, Yuqing Meng, Qiaoli Shi, Guang Han, Xijun Wang, Jigang Wang

“…Here, we present a multifunctional pH-sensitive core-shell nanoparticle containing quercetin (QCT), termed AHA@MnP/QCT NPs, designed for the simultaneous diagnosis and treatment of non-small cell lung cancer. …”
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Effective cancer immunotherapy combining mRNA-encoded bispecific antibodies that induce polyclonal T cell engagement and PD-L1-dependent 4-1BB costimulation by Oana Hangiu, Oana Hangiu, Oana Hangiu, Rocío Navarro, Susana Frago, Laura Rubio-Pérez, Laura Rubio-Pérez, Laura Rubio-Pérez, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Laura Díez-Alonso, Laura Díez-Alonso, Laura Díez-Alonso, Marina Gómez-Rosel, Marina Gómez-Rosel, Marina Gómez-Rosel, Noelia Silva-Pilipich, Noelia Silva-Pilipich, Lucía Vanrell, Cristian Smerdou, Cristian Smerdou, Kenneth A. Howard, Laura Sanz, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Marta Compte

“…Pharmacokinetic studies demonstrated sustained expression of both bispecific antibodies following intravenous administration of the mRNAs formulated using a polymer/lipid-based nanoparticle (LNP) but different pharmacokinetic profiles, shorter for the TCE and longer for the PD-L1 x 4-1BB. …”
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Nanotechnology-Enhanced Controlled Release Systems in Topical Therapeutics by Yohannes Teklehaimanot

“…This review examines recent advances, challenges, and prospects of controlled-release nanoparticles for topical drug delivery. Various types of nanoparticles, including lipid nanoparticles, nanoemulsions, nanoemulgels, cubosome liposomes, poly-meric nanoparticles, solid-lipid nanoparticles, lipid-polymer hybrid nanoparticles, and nanostructured lipid carriers, carbon nanotubes, nanocomposites, and protein nanoparticles have been explored for their ability to encapsulate drugs, prolong drug release, and enhance skin permeation as well as improve therapeutic outcomes. …”
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Preparation of Polymeric Nanomaterials Using Emulsion Polymerization by Satyajeet Sahoo, Anitha Gopalan, S. Ramesh, P. Nirmala, G. Ramkumar, S. Agnes Shifani, Ram Subbiah, J. Isaac JoshuaRamesh Lalvani

“…Since nanoparticles were used in medical and biotechnological fields, there is a great demand in the preparation of nanoparticles. …”
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Preparation and Characterization of Nickel Ferrite-SiO2/Ag Core/Shell Nanocomposites by I. G. Blanco-Esqueda, G. Ortega-Zarzosa, J. R. Martínez, A. L. Guerrero

“…Magnetic composites with silver nanoparticles bonded to their surface were successfully prepared using a simple chemical method. …”
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Green metal nanotechnology in monogastric animal health: current trends and future prospects — A review by Sungyeon Chin, Mohammad Moniruzzaman, Elena Smirnova, Do Thi Cat Thoung, Anjana Sureshbabu, Adhimoolam Karthikeyan, Dong I. Lee, Taesun Min

“…Nanoparticles can be synthesised by physical, chemical and biological processes. …”
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A Systematic Review of Spironolactone Nano-Formulations for Topical Treatment of Skin Hyperandrogenic Disorders and Chronic Wounds by Saedah Dereiah, Muhammad Usman Ghori, Barbara R. Conway

“…This review systematically examines recent advancements in SP-loaded nanocarriers, including lipid nanoparticles (LNPs), vesicular nanoparticles (VNPs), polymeric nanoparticles (PNPs), and nanofibers (NFs). …”
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The Modification of Sodium Polyacrylate Water Solution Cooling Properties by AL2O3 by Wojciech Gęstwa, Małgorzata Przyłęcka

“…Obtaining the smallest wetting angle for the medium containing nanoparticles suggests that the heat transfer to the cooling medium is larger. …”
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Investigation on the Mechanical Properties of Powder Metallurgy-Manufactured AA7178/ZrSiO4 Nanocomposites by R. Srinivasan, S. Karunakaran, M. Hariprabhu, R. Arunbharathi, S. Suresh, S. Nanthakumar, S. K. H. Ahammad, S. Mayakannan, Mani Jayakumar

“…The current study used a ball milling to mechanically AA7178 powder and strengthened with zirconium silicate (ZrSiO4) nanoparticles. In addition, the AA7178 matrix was ball-milled to distribute the ZrSiO4 nanoparticles throughout the material. …”
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Investigating the Feasibility of Using Concrete Containing Waste Paper Sludge Ash and Pozzolanic Materials to Repair Bridge Stub Columns by F. Delaram, Y. Mohammadi, M. R. Adlparvar

“…In the present study, several concrete stub columns were built as bridge columns and retrofitted with concrete jackets containing waste paper sludge ash, silica nanoparticles, aluminium oxide nanoparticles, and acrylic resin. …”
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