Alkaloids of narrow-leaved lupine as a factor determining alternative ways of the crop’s utilization and breeding by M. A. Vishnyakova, A. V. Kushnareva, T. V. Shelenga, G. P. Egorova

“…The first varieties of scientific breeding were created only in the 1930s after the appearance of low-alkaloid mutants. Despite wide prospects for use in various areas of the national economy, unstable productivity and susceptibility to diseases hinder the production of this crop. …”
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Characterization of YdgH: a mediator of beta-lactam susceptibility in Enterobacterales by Jacob E. Lazarus, Matthew K. Waldor, David C. Hooper

“…Finally, we delineate the results of a genetic screen for spontaneous mutants that complement the detergent susceptibility phenotype, the results of which may fuel the further studies that are necessary to determine the precise role YdgH plays in bacterial physiology.IMPORTANCEBeta-lactams such as penicillins and cephalosporins are the most commonly prescribed antibiotics for serious bacterial infections. …”
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Exploitation of the genetic potential of <i>Thinopyrum</i> and <i>Agropyron</i> genera to protect wheat from diseases and environmental stresses by L. Ya. Plotnikova, V. V. Knaub

“…Approaches to improving the agronomic properties of introgression breeding material (the use of irradiation, ph-mutants and compensating Robertsonian translocations) were described. …”
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Cell-associated flagella enhance the protection conferred by mucosally-administered attenuated Salmonella Paratyphi A vaccines. by Orit Gat, James E Galen, Sharon Tennant, Raphael Simon, William C Blackwelder, David J Silverman, Marcela F Pasetti, Myron M Levine

“…<h4>Results</h4>Mucosal (intranasal) immunization of mice with strain CVD 1901 expressing cell-associated flagella conferred superior protection (vaccine efficacy [VE], 90%) against a lethal intraperitoneal challenge, compared with the flagellin monomer-exporting mutants CVD 1901K (30% VE) or CVD 1901D (47% VE). …”
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Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology in Escherichia coli by Christopher W. Hamm, Michael J. Gray

“…Disruption of either (p)ppGpp or polyP synthesis led to the formation of filamentous cells, but simultaneous disruption of both pathways resulted in cells with heterogenous cell morphologies, including highly branched cells, severely mislocalized Z-rings, and cells containing substantial void spaces. These mutants also failed to grow when nutrients were limited, even when amino acids were added. …”
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Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsi... by Nian Anwar Nasralddin, Mehri Haeili, Sasan Karimzadeh, Fatemeh Alsahlani

“…Our study demonstrated the capacity of chloramphenicol and tetracycline exposure for selection of mutants which revealed tigecycline resistance/decreased susceptibility mostly mediated by active efflux mechanism. …”
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Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India by Avik Biswas, Rajesh Panigrahi, Partha Kumar Chandra, Arup Banerjee, Sibnarayan Datta, Manisha Pal, Subhashish Chakraborty, Prasun Bhattacharya, Sekhar Chakrabarti, Runu Chakravarty

“…This study was aimed to characterize the OBI strains circulating among the blood donors and to estimate the risk associated with the prevailing viral variants/mutants. Blood samples from 2195 voluntary blood donors were included in the study. …”
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Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora by Qianqian Liu, Kexin Jiang, Shipeng Duan, Na Zhao, Yanmei Shen, Lirong Zhu, Ke-Qin Zhang, Jinkui Yang

“…In particular, spore yield, spore germination rate, traps, and nematode predation efficiency were remarkably decreased in the mutants. Phenotypic and transcriptomic analyses revealed that AoMsn2 is essential for fatty acid metabolism and autophagic pathways. …”
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Impacts of Pta-AckA pathway on CPS biosynthesis and type 3 fimbriae expression in Klebsiella pneumoniae by Tien-Huang Lin, Chen-Yu Wang, Chien-Chen Wu, Ching-Ting Lin

“…However, the role of the Pta-AckA pathway, which regulates AcP levels, in K. pneumoniae pathogenesis remains unclear. Methods: Deletion mutants lacking the pta and ackA, involved in AcP production and hydrolysis, were generated in K. pneumoniae CG43S3. …”
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TALome and phenotypic analysis of Pakistani Xanthomonas oryzae pv. oryzae population revealed novel virulent TALEs contributing to bacterial blight of rice by Syed Mashab Ali Shah, Rafia Ahsan, Linlin Liu, Ying Li, Qi Wang, Yong Wang, Jiali Yan, Moein Khojasteh, Xiameng Xu, Zhengyin Xu, Awais Rasheed, Muhammad Zakria, Gongyou Chen

“…In contrast, paddy trails on NILs containing single, double, and triple mutants of OsSWEET11a, OsSWEET13, and OsSWEET14 in the effector binding elements (EBEs) of cv. …”
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Involvement of p38 MAPK and MAPKAPK2 in promoting cell death and the inflammatory response to ischemic stress associated with necrotic glioblastoma by Soo Yeon Kim, Miaolu Tang, Stephen Y. Chih, Jessica Sallavanti, Yan Gao, Zhiqiang Qiu, Hong-Gang Wang, Wei Li

“…We found that glioblastoma (GBM) cells expressing patient-derived Kirsten rat sarcoma (KRAS) or phosphoinositide-3-kinase (PI3K) active mutants showed enhanced cell death under ischemia-mimetic conditions in vitro and were more likely to develop into necrotic tumors in vivo. …”
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Determination of streptococcus mutans retention in acidic and neutral pH artificial saliva environment of all-ceramic materials with different surface treatment by Yeliz Hayran, Süha Kuşcu, Ali Aydın

“…This study aims to produce four different all-ceramic materials in a single session with CAD/CAM devices and apply two different surface finishing processes, glazing and polishing, and then determine the retention of Streptococcus mutants on the surfaces of the materials in saliva with varying levels of acidity. …”
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The transcription factor dFOXO controls the expression of insulin pathway genes and lipids content under heat stress in <i>Drosophila melanogaster</i> by M. A. Eremina, P. N. Menshanov, O. D. Shishkina, N. E. Gruntenko

“…Both mutations caused an increase in these parameters under normal conditions and prevented decrease in total lipids content following heat stress observed in the control strain. In mutants, feeding intensity was increased under normal conditions; and decreased following short-term heat stress in all studied strains for the first 24 h of observation, and in dilp641 strain, for 48 h. …”
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The alphavirus determinants of intercellular long extension formation by Caroline K. Martin, Judy J. Wan, Peiqi Yin, Thomas E. Morrison, William B. Messer, Vanessa Rivera-Amill, Jonathan R. Lai, Nina Grau, Félix A. Rey, Thérèse Couderc, Marc Lecuit, Margaret Kielian

“…Here, we used well-characterized virus mutants and monoclonal antibodies with known epitopes to dissect the virus requirements for ILE formation. …”
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Functional and Structural Consequences of Nine CYP21A2 Mutations Ranging from Very Mild to Severe Effects by Débora de Paula Michelatto, Leif Karlsson, Ana Letícia Gori Lusa, Camila D’Almeida Mgnani Silva, Linus Joakim Östberg, Bengt Persson, Gil Guerra-Júnior, Sofia Helena Valente de Lemos-Marini, Michela Barbaro, Maricilda Palandi de Mello, Svetlana Lajic

“…The maximum velocity was decreased for all mutants except for p.Leu12Met. We conclude that p.Leu12Met is a normal variant; the mutations p.Arg16Cys, p.Ser101Asn, p.Ser202Gly, p.Pro267Leu, and p.Thr450Met could be associated with very mild nonclassic CAH, and the mutations p.Ser113Phe, p.Gln389_Ala391del, and p.Thr450Pro are associated with classic CAH. …”
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The Rickettsia actin-based motility effectors RickA and Sca2 contribute differently to cell-to-cell spread and pathogenicity by Cuong J. Tran, Zahra Zubair-Nizami, Ingeborg M. Langohr, Matthew D. Welch

“…Using live cell imaging of rickA::Tn and sca2::Tn mutants, we discovered both RickA and Sca2 contribute to different modes of cell-to-cell spread. …”
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Phage-mediated virulence loss and antimicrobial susceptibility in carbapenem-resistant Klebsiella pneumoniae by Yanshuang Yu, Mengzhu Wang, Liuying Ju, Minchun Li, Mengshi Zhao, Hui Deng, Christopher Rensing, Qiu E. Yang, Shungui Zhou

“…In contrast, phages evolved under the selection pressure against Kp2092 mutants and exhibited enhanced bacterial killing potency, targeting mutations in phage tail proteins gp12 and gp17. …”
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Revealing the biological features of the axolotl pancreas as a new research model by Hui Ma, Hui Ma, Hui Ma, Guangcong Peng, Yan Hu, Binbin Lu, Binbin Lu, Yiying Zheng, Yingxian Wu, Weimin Feng, Yu Shi, Xiangyu Pan, Li Song, Ina Stützer, Yanmei Liu, Jifeng Fei, Jifeng Fei, Jifeng Fei

“…To assess pancreatic gene function, Pdx1 mutants were generated using CRISPR/Cas9-mediated gene editing, and their effects on pancreatic morphology, endocrine cell populations, and glucose homeostasis were analyzed.ResultsThe axolotl pancreas contains all known pancreatic cell types and develops from dorsal and ventral buds. …”
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Pseudovibriamides from Pseudovibrio marine sponge bacteria promote flagellar motility via transcriptional modulation by Yitao Dai, Vitor Lourenzon, Laura P. Ióca, Dua Al-Smadi, Lydia Arnold, Ian McIntire, Roberto G. S. Berlinck, Alessandra S. Eustáquio

“…RNA-sequencing transcriptomic analyses of strains having different compositions of pseudovibriamides suggested that both pseudovibriamides A and B affect genes potentially involved in motility, and that a compensatory mechanism is at play in mutants that produce only pseudovibriamide A, resulting in comparable flagellar motility as the wild type. …”
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Bifurcation analysis of multistability and hysteresis in a model of HIV infection by I. V. Mironov, M. Yu. Khristichenk, Yu. M. Nechepurenko, D. S. Grebennikov, G. A. Bocharov

“…The model describes the most important blocks of the processes of interaction between viruses and the human body, namely, the spread of infection in productively and latently infected cells, the appearance of viral mutants and the development of the T cell immune response. …”
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