β-Glucosidase VmGlu1 is required for toxin production and pathogenicity of Valsa mali by Xinyue Cui, Dewan Zhang, Liyong Gao, Na Liu, Sen Lian, Weichao Ren, Baohua Li, Caixia Wang

“…Furthermore, we verified that VmGlu1 and VmGlu2 exhibit functional redundancy in pycnidia production, toxin-related β-glucosidase activity, and pathogenicity by generating double-deletion mutants. The results of this study provide valuable insights into the roles of VmGlus in the interaction of V. mali and apple during the infection process.…”
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A new type III effector from Bradyrhizobium sp. DOA9 encoding a putative SUMO-protease blocks nodulation in Arachis hypogaea L. by Beedou Aphaiso, Pongdet Piromyou, Pakpoom Boonchuen, Pongpan Songwattana, Jenjira Wongdee, Teerana Greetatorn, Kamonluck Teamtisong, Alicia Camuel, Panlada Tittabutr, Nantakorn Boonkerd, Eric Giraud, Neung Teaumroong

“…While the DOA9 strain and the two mutants of SUMO-proteases, p0490 and p0871, induced inefficient nodulation in A. hypogaea, the mutation of SUMO-proteases SkP48 or p0903 promoted efficient symbiosis comparable to the type strain Bradyrhizobium arachidis CCBAU051107. …”
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“Sichuanvirus”, a novel bacteriophage viral genus, able to lyse carbapenem-resistant Klebsiella pneumoniae by Juan Li, Qingqing Fang, Huan Luo, Yan Feng, Yu Feng, Zhiyong Zong

“…We also obtained three bacterial mutants resistant to the phage and performed genome sequencing for them. …”
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Trichostatin A promotes de novo shoot regeneration from Arabidopsis root explants via a cytokinin related pathway by Su Hyun Park, Yu Jeong Jeong, Soyoung Kim, Jiyoung Lee, Cha Young Kim, Jae Cheol Jeong

“…Concomitantly, type-B ARABIDOPSIS RESPONSE REGULATOR (ARR) 10 and 12, which are key regulators of cytokinin signaling, were upregulated in TSA-treated callus, whereas the downstream targets of type-B ARRs, such as ARR5, ARR7, and ARR15, were significantly upregulated during shoot regeneration. Furthermore, mutants deficient in ARR10 and ARR12 showed diminished responsiveness to shoot regenerative capacity, a phenotype that was enhanced by TSA treatment. …”
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Aportes a la Técnica de Insecto Estéril: Una cepa de Anastrepha fraterculus (Diptera: Tephritidae) con maduración sexual temprana by P.A. Peralta, F.H. Milla, J.L. Cladera

“…Asimismo, se estudió el efecto del tratamiento tópico con hormona juvenil (HJ) sobre la madura- ción sexual en estos machos mutantes. El tiempo que tardan los individuos en alcanzar la madurez sexual fue determinado mediante observaciones del comportamiento sexual, donde machos de distintas edades fueron confrontados con hembras vírgenes sexualmente maduras. …”
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Separable roles of the DNA damage response kinase Mec1ATR and its activator Rad24RAD17 during meiotic recombination. by Margaret R Crawford, Jon A Harper, Tim J Cooper, Marie-Claude Marsolier-Kergoat, Bertrand Llorente, Matthew J Neale

“…Such effects are similar to, but less extreme than, the phenotype of 'ZMM' mutants such as zip3Δ, and may be driven by reductions in the proportion of interfering COs. …”
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Investigating a role for PUB17 and PUB16 in the self‐incompatibility signaling pathway in transgenic Arabidopsis thaliana by Paula K. S. Beronilla, Daphne R. Goring

“…Two close ARC1 homologs, PUB17 and PUB16, were selected, and (Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) technology was used to generate pub17 and pub16 mutations in the C24 accession. These mutants were then crossed into the transgenic A. thaliana SI‐C24 line and their potential impact on SI pollen rejection was investigated. …”
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Palmitic Acid Accelerates Endothelial Cell Injury and Cardiovascular Dysfunction via Palmitoylation of PKM2 by Yu He, Senlin Li, Lujing Jiang, Kejue Wu, Shanshan Chen, Linjie Su, Cui Liu, Peiqing Liu, Wenwei Luo, Shilong Zhong, Zhuoming Li

“…Palmitoyl‐proteomics analysis identifies pyruvate kinase isozyme type M2 (PKM2) as the palmitoylated protein responsible for PA‐induced endothelial injury, and Cys31 as the predominant palmitoylated site. PKM2‐C31S mutants (cysteine replaced by serine) prevents PA‐induced endothelial injury. …”
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GENI as an AMPK Activator Binds α and γ Subunits and Improves the Memory Dysfunction of Alzheimer’s Disease Mouse Models via Autophagy and Neuroprotection by Ying Wang, Lanjie Li, Danni Chen, Jiaheng Shan, Meijuan Yi, Hiroyuki Osada, Minoru Yoshida, Lan Xiang, Jianhua Qi

“…Furthermore, the Western blot, small interfering RNA (siRNA), drug affinity responsive target stability (DARTS), cellular thermal shift assay (CESTA), liquid chromatography-tandem mass spectrometry (LC/MS-MS), adenosine 5′-monophosphate-activated protein kinase (AMPK) mutants and surface plasmon resonance (SPR) analysis were utilized to clarify the mechanism of action and identify target protein of this molecule. …”
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Qualitative and quantitative characteristics of black patch trait in Oujiang color common carp by Ke Jing, Huifan Chen, Nusrat Hasan Kanika, Penghui Zhang, Roland Nathan Mandal, Chunxiao Zeng, Zhiyi Zhang, Jun Wang, Chenghui Wang

“…Gene editing on Ednrb by using the CRISPR/Cas9 system showed that the mutants displayed a significantly reduced number of black patches in comparison to the wild-type RB, accompanied by noticeably smaller melanocytes. …”
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Essential Roles of PKA, iNOS, CD95/CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents by Bianca de Luca, Pedro Xavier-Elsas, Mônica Barradas, Ricardo A. Luz, Túlio Queto, Carla Jones, Maria Augusta Arruda, Thiago Mattar Cunha, Fernando Queiroz Cunha, Maria Ignez Gaspar-Elsas

“…Bone-marrow collected from BALB/c mice, or from iNOS-, CD95-, or CD95L-deficient mutants (and wild-type controls), was cultured with interleukin-5 (IL-5), alone or associated with PGE2, cAMP-inducing/mimetic agents, caspase inhibitor zVAD-fmk, iNOS inhibitor aminoguanidine, or combinations thereof, and eosinopoiesis was evaluated at various times. …”
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Rational design of a triple-type HPV53/56/66 vaccine with one preferable base particle incorporating two identified immunodominant sites by Ciying Qian, Jie Chen, Yurou Yang, Yihan Lu, Tianyu Ren, Yanan Jiang, Yang Huang, Xin Chi, Shuyue Zhang, Chengzong Zhang, Kewei Li, Jingjia Shen, Sibo Zhang, Daning Wang, Lizhi Zhou, Tingting Li, Qingbing Zheng, Hai Yu, Ying Gu, Ningshao Xia, Shaowei Li

“…It introduces an effective method for their soluble expression, as well as that of their mutants, within an Escherichia coli expression system. …”
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Mode of action of silver-based perovskite against Gram-negative bacteria by Fereshteh Fani, Cyrus Talebpour, Philippe Leprohon, Hossein Salimnia, Houshang Alamdari, Marc Ouellette

“…This was concluded from the characterization of mutants resistant to AgNbO3 NPs that showed cross-resistance to AgNO3, impaired reactive oxygen species production and lipid peroxidation, and harbored a key mutation in a two-component regulatory system regulating an Ag+ efflux pump. …”
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Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion by Mehul Vora, Jonathan Dietz, Zachary Wing, Karen George, Jun Kelly Liu, Christopher Rongo, Cathy Savage-Dunn

“…Here, we identify functional binding sites for SMA-3/Smad and its transcriptional partner SMA-9/Schnurri based on ChIP-seq peaks (identified by modEncode) and expression differences of nearby genes identified from RNA-seq analysis of corresponding mutants. We found that SMA-3 and SMA-9 have both overlapping and unique target genes. …”
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Standardization of the Agar Plate Method for Bacteriophage Production by Su Jin Jo, Young Min Lee, Kevin Cho, Seon Young Park, Hyemin Kwon, Sib Sankar Giri, Sung Bin Lee, Won Joon Jung, Jae Hong Park, Mae Hyun Hwang, Da Sol Park, Eun Jae Park, Sang Wha Kim, Jin Woo Jun, Sang Guen Kim, Ji Hyung Kim, Se Chang Park

“…The model’s validation showed a strong inverse correlation between plaque size and minimum-lawn cell clearing PFUs (MCPs; R² = 98.91%) and identified an optimal inoculum density that maximizes yield while minimizing the evolution of resistant mutants. These results highlight that the PSC model offers a standardized and scalable method for efficient phage production, which is crucial for personalized therapy and AMR management. …”
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Optimization of a microarray for fission yeast by Dong-Uk Kim, Minho Lee, Sangjo Han, Miyoung Nam, Sol Lee, Jaewoong Lee, Jihye Woo, Dongsup Kim, Kwang-Lae Hoe

“…A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. …”
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DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage by Qiao Yang, Yaya Feng, Lizhen Liu, Linlin Yang, Mingshu Wang, Ying Wu, Bin Tian, Xumin Ou, Renyong Jia, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Juan Huang, Di Sun, Yu He, Zhen Wu, Ling Zhang, Yanling Yu, Anchun Cheng

“…Subsequently, several single-point mutants were constructed to identify the vital amino acids within the NLS. …”
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Sunflower lines originated from varieties with high oil content in their seed by V. A. Gavrilova, I. N. Anisimova, T. G. Stupnikova

“…Pustovoit and his associates, and the first high-yielding cultivars ('Peredovik', 'VNIIMK 8883') with high oil content in seeds (50-60%) were produced. Mutants with modified fatty acid composition were obtained, and high oleic cultivar 'Pervenets' was created. …”
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Plant cell wall and the mechanisms of resistance to pathogens by O. G. Smirnova, A. V. Kochetov

“…Plants also can evaluate the cell wall state to compensate for imbalances and deficiencies. For instance, mutants with cellulose deficiency may have a higher lignification rate and a stronger stress response. …”
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Inhibition of the anti-apoptotic protein BCL2 in EML4-ALK cell models as a second proposed therapeutic target for non-small cell lung cancer. by Richard Junior Zapata Dongo, Diletta Fontana, Luca Mologni, Juan Enrique Faya Castillo, Stefany Fiorella Infante Varillas

“…In addition, our analysis using bioinformatics indicated that ABT-199 not only targets BCL2 but also binds to the active site of all ALK mutants, it was contrasted by in vitro ALK kinase activity inhibition by ABT-199 (5.5 μM). …”
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