Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.) by Athanasios S. Tsaftaris, Alexios N. Polidoros, Konstantinos Pasentsis, Apostolos Kalivas

“…In order to uncover and understand the molecular mechanisms controlling flower development in cultivated crocus and its relative wild progenitor species, and characterize a number of crocus flower mutants, we have cloned and characterized different, full-length, cDNA sequences encoding MADS-box transcription factor proteins involved in flower formation.…”
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Biosynthesis and Role of N-Linked Glycosylation in Cell Surface Structures of Archaea with a Focus on Flagella and S Layers by Ken F. Jarrell, Gareth M. Jones, Divya B. Nair

“…The N-linked glycosylation system is not essential for any of M. voltae, M. maripaludis, or H. volcanii, as demonstrated by the successful isolation of mutants carrying deletions in the oligosaccharyltransferase gene aglB (a homologue of the eukaryotic Stt3 subunit of the oligosaccharyltransferase complex). …”
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A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> by Xiaoran Du, Yan Zeng, Yiying Li, Qin Peng, Jianqiang Miao, Xili Liu

“…The <i>Ps495620</i>-knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. …”
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TransLeish: Identification of membrane transporters essential for survival of intracellular Leishmania parasites in a systematic gene deletion screen by Andreia Albuquerque-Wendt, Ciaran McCoy, Rachel Neish, Ulrich Dobramysl, Çağla Alagöz, Tom Beneke, Sally A. Cowley, Kathryn Crouch, Richard J. Wheeler, Jeremy C. Mottram, Eva Gluenz

“…A total of 312 protein components of small molecule carriers, ion channels and pumps were identified and targeted in a CRISPR-Cas9 gene deletion screen in the promastigote form, yielding 188 viable null mutants. Forty transporter deletions caused significant loss of fitness in macrophage and mouse infections. …”
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Congenital Anomalies of the Kidney and Urinary Tract: A Genetic Disorder? by Ihor V. Yosypiv

“…In this paper, we will focus on the knowledge derived from the study of syndromic and non-syndromic forms of CAKUT in humans and mouse mutants to discuss the role of genetic, epigenetic, and in utero environmental factors in the pathogenesis of non-syndromic forms of CAKUT in children with particular emphasis on the genetic contributions to CAKUT.…”
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L’après-tourisme revisité by Philippe Bourdeau

“…Elle se présente comme une notion composite destinée à articuler entre elles trois modalités mutantes du tourisme moderne : post-tourisme(s), trans-tourisme et hypertourisme.Le texte s’exerce à une approche réflexive de la genèse, des étapes, de la réception et des évolutions de cette proposition. …”
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The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis th... by Weidong Zhu, Jie Qian, Yingke Hou, Luke R. Tembrock, Liyun Nie, Yi-Feng Hsu, Yong Xiang, Yi Zou, Zhiqiang Wu

“…Based on accurate long-read sequencing results, ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA (mtDNA) and plastid DNA (ptDNA), accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes. …”
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Blobby is a synaptic active zone assembly protein required for memory in Drosophila by J. Lützkendorf, T. Matkovic-Rachid, S. Liu, T. Götz, L. Gao, O. Turrel, M. Maglione, M. Grieger, S. Putignano, N. Ramesh, T. Ghelani, A. Neumann, N. Gimber, J. Schmoranzer, A. Stawrakakis, B. Brence, D. Baum, Kai Ludwig, M. Heine, T. Mielke, F. Liu, A. M. Walter, M. C. Wahl, S. J. Sigrist

“…Functionally, blobby mutants show diminished evoked synaptic currents due to reduced synaptic vesicle release probability and fewer functional release sites. …”
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COVID-19 Whole-Genome Resequencing with Redundant Tiling PCR and Subtract-Based Amplicon Normalization Successfully Characterized SARS-CoV-2 Variants in Clinical Specimens by Tatsuki Sugi, Mizanur Rahman, Rummana Rahim, Abu Hasan, Naoko Kawai, Kyoko Hayashida, Junya Yamagishi

“…With an increasing number of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) sequences gathered worldwide, we recognize that deletion mutants and nucleotide substitutions that may affect whole-genome sequencing are accumulating. …”
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ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics. by Michael D Stubenvoll, Jeffrey C Medley, Miranda Irwin, Mi Hye Song

“…Depleting ATX-2 results in embryonic lethality and cytokinesis failure, and restores centrosome duplication to zyg-1 mutants. In this pathway, SZY-20 promotes ATX-2 abundance, which inversely correlates with centrosome size. …”
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Glycogen homeostasis and mitochondrial DNA expression require motor neuron to muscle TGF-β/Activin signaling in Drosophila by Heidi Bretscher, Michael B. O’Connor

“…Lastly, expressing an activated form of the type I receptor Baboon in muscle restores both glycogen and mtDNA levels in actβ mutants, thereby confirming a direct link between Actβ signaling, glycogen homeostasis, and mtDNA expression factors.…”
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Conserved role of spike S2 domain N-glycosylation across betacoronaviruses by Qi Yang, Anju Kelkar, Balaji Manicassamy, Sriram Neelamegham

“…Studies were performed with 31 Asn-to-Gln mutants, betacoronavirus virus-like particles and single-cycle viral replicons. …”
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Full-length inhibitor protein is the most effective to perturb human dUTPase activity by Bianka Kőhegyi, Zoé S. Tóth, Enikő Gál, Máté Laczkovich, András Benedek, Beáta G. Vértessy, Kinga Nyíri

“…Towards this end we determined the 3D crystal structure of the crystallizable amino terminal domain of inhibitor protein, named StlNT in complex with the human dUTPase and designed several point mutants based on the structure to improve the inhibition effectivity. …”
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Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis by Kylie R. VanDerMolen, Martin A. Newman, Peter C. Breen, Yunjing Gao, Laura A. Huff, Robert H. Dowen

“…We demonstrate, using C. elegans and mouse hepatocytes, that Hh metabolic regulation does not occur through the canonical Hh transcription factor TRA-1/GLI, but rather via non-canonical signaling that engages mammalian target of rapamycin complex 2 (mTORC2). Hh mutants display impaired lipid homeostasis, decreased growth, and upregulation of autophagy factors, mimicking loss of mTORC2. …”
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Cooperative condensation of RNA-DIRECTED DNA METHYLATION 16 splicing isoforms enhances heat tolerance in Arabidopsis by Jing Ma, Shuai Li, Tengyue Wang, Zhen Tao, Shijie Huang, Ning Lin, Yibing Zhao, Chuanhong Wang, Peijin Li

“…Here, we identify a heat-responsive gene in Arabidopsis thaliana, RNA-DIRECTED DNA METHYLATION 16 (RDM16), which encodes a pre-mRNA splicing factor. Knockout mutants of RDM16 are hypersensitive to heat stress, which is associated with impaired splicing of the mRNAs of 18 out of 20 HEAT SHOCK TRANSCRIPTION FACTOR (HSF) genes. …”
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Characterization of a novel covS SNP identified in Australian group A Streptococcus isolates derived from the M1UK lineage by Johanna Richter, Amanda J. Cork, Yvette Ong, Nadia Keller, Andrew J. Hayes, Mark A. Schembri, Amy V. Jennison, Mark R. Davies, Kate Schroder, Mark J. Walker, Stephan Brouwer

“…These findings underscore the need for comprehensive analyses of covRS mutants to fully elucidate their contribution to M1UK virulence and persistence.…”
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Adhesion of Neisseria meningitidis to dermal vessels leads to local vascular damage and purpura in a humanized mouse model. by Keira Melican, Paula Michea Veloso, Tiffany Martin, Patrick Bruneval, Guillaume Duménil

“…The adhesive properties of the type IV pili of N. meningitidis were found to be the main mediator of association with the dermal microvessels in vivo. Bacterial mutants with altered type IV pili function also did not trigger inflammation or lead to vascular damage. …”
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Acetylation of the yeast Hsp40 chaperone protein Ydj1 fine-tunes proteostasis and translational fidelity. by Siddhi Omkar, Megan M Mitchem, Joel R Hoskins, Courtney Shrader, Jake T Kline, Nitika, Luca Fornelli, Sue Wickner, Andrew W Truman

“…By mutating acetylation sites on Ydj1's J-domain to either abolish or mimic constitutive acetylation, we observed that preventing acetylation had no noticeable phenotypic impact, whereas acetyl-mimic mutants exhibited various defects indicative of impaired Ydj1 function. …”
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scCamAge: A context-aware prediction engine for cellular age, aging-associated bioactivities, and morphometrics by Vishakha Gautam, Subhadeep Duari, Saveena Solanki, Mudit Gupta, Aayushi Mittal, Sakshi Arora, Anmol Aggarwal, Anmol Kumar Sharma, Sarthak Tyagi, Rathod Kunal Pankajbhai, Arushi Sharma, Sonam Chauhan, Shiva Satija, Suvendu Kumar, Sanjay Kumar Mohanty, Juhi Tayal, Nilesh Kumar Dixit, Debarka Sengupta, Anurag Mehta, Gaurav Ahuja

“…We present scCamAge, an advanced context-aware multimodal prediction engine that co-leverages image-based cellular spatiotemporal features at single-cell resolution alongside cellular morphometrics and aging-associated bioactivities such as genomic instability, mitochondrial dysfunction, vacuolar dynamics, reactive oxygen species levels, and epigenetic and proteasomal dysfunctions. scCamAge employed heterogeneous datasets comprising ∼1 million single yeast cells and was validated using pro-longevity drugs, genetic mutants, and stress-induced models. scCamAge also predicted a pro-longevity response in yeast cells under iterative thermal stress, confirmed using integrative omics analyses. …”
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Promoter capture Hi-C identifies promoter-related loops and fountain structures in Arabidopsis by Dingyue Wang, Suxin Xiao, Jiayue Shu, Lingxiao Luo, Minqi Yang, Myriam Calonje, Hang He, Baoxing Song, Yue Zhou

“…Moreover, fountain strength is positively correlated with the number of promoter-related loops, and the maintenance of these loops is linked to H3K4me3. In atxr3 mutants, which lack the major H3K4me3 methyltransferases in Arabidopsis, the number of promoter-related loops at fountains is reduced, leading to upregulation of fountain-regulated genes. …”
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