Community-Acquired Pneumonia and Empyema Caused by Citrobacter koseri in an Immunocompetent Patient by Miguel Angel Ariza-Prota, Ana Pando-Sandoval, Marta García-Clemente, Ramón Fernández, Pere Casan

“…The infections caused by Citrobacter are difficult to treat with usual broad spectrum antibiotics owing to rapid generation of mutants and have been associated with high death rates in the past. …”
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Phenotypic effects of different doses of physical and chemical mutagens in cotton plants by H.M Winkler, A.E. Cereijo, G.J. Scarpin, P.N. Dileo, R.J. Muchut, R.A. Roeschlin, F.G. Lorenzini, M.J. Paytas, Alejandra M. Landau

“…Las dosis deben optimizarse para aumentar las posibilidades de generar mutantes y lograr una población de plantas mutagenizadas que permita una selección exitosa de mutantes de interés. …”
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Cell Type Specific Suppression of Hyper-Recombination by Human RAD18 Is Linked to Proliferating Cell Nuclear Antigen K164 Ubiquitination by Colette B. Rogers, Wendy Leung, Ryan M. Baxley, Rachel E. Kram, Liangjun Wang, Joseph P. Buytendorp, Khoi Le, David A. Largaespada, Eric A. Hendrickson, Anja-Katrin Bielinsky

“…Our analyses found that loss of RAD18 in HCT116, but neither hTERT RPE-1 nor DLD1 cell lines, resulted in elevated sister chromatid exchange, gene conversion, and gene targeting, i.e., HCT116 mutants were hyper-recombinogenic (hyper-rec). Interestingly, these phenotypes were linked to RAD18’s role in PCNA K164 ubiquitination, as HCT116 <i>PCNA^K164R/+</i> mutants were also hyper-rec, consistent with previous studies in <i>rad18</i>^−/− and <i>pcna^K164R</i> avian DT40 cells. …”
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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

“…Summary: Characterization of zebrafish mutants is routinely time consuming as morphological phenotypes can be subtle or variable, thus hampering robust quantification. …”
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THE DEVELOPMENT OF A GENE POOL OF WHEAT AND TRITICALE AND ITS USE IN BREEDING by P. I. Stepochkin

“…In addition, a collection of spontaneous spring mutants isolated from populations of winter crops is being developed. …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

“…The anticoagulation assay showed that the four P2′ single-point mutants still had apparent inhibitory anticoagulation activity that selectively inhibited the human intrinsic coagulation pathway and had no influence on the extrinsic coagulation pathway or common coagulation pathway, which indicated that the single-point mutants had minimal effects on the anticoagulation activity of BF9-N17K. …”
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Genome-wide identification of novel flagellar motility genes in Pseudomonas syringae pv. tomato DC3000 by Zichu Yang, Tyler Helmann, Maël Baudin, Maël Baudin, Maël Baudin, Karl J. Schreiber, Karl J. Schreiber, Zhongmeng Bao, Paul Stodghill, Paul Stodghill, Adam Deutschbauer, Adam Deutschbauer, Jennifer D. Lewis, Jennifer D. Lewis, Bryan Swingle, Bryan Swingle

“…In this study, we used a competitive selection to enrich for mutants with altered swimming motility and used random barcode transposon-site sequencing (RB-TnSeq) to identify genes with significant roles in swimming motility. …”
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Coronavirus Vaccines in Abundance — What Then? by Dieter Cassel, Volker Ulrich

“…However, in order to achieve herd immunity, which is likely to require an immunisation rate of around 80 % due to the highly infectious mutants, approximately 65 million people need to be vaccinated. …”
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FEATURES OF THE FORMATION OF PRODUCTIVITY AND ADAPTIVE REACTIONS IN LEGUMINOUS CROP VARIETIES WITH RECESSIVE ALLELES OF GENES by S. N. Agarkova, N. E. Novikova, R. V. Belyaeva, E. V. Golovina, Zh. A. Belyaeva, Z. R. Tsukanova, N. I. Mit’kina

“…Researches were carried out on varieties, hybrids, isogenic lines and mutants of peas of various use developed from the beginning of the 1980s up to 2013. …”
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HP1 Promotes the Centromeric Localization of ATRX and Protects Cohesion by Interfering Wapl Activity in Mitosis by Erchen Zhang, Lei Peng, Kejia Yuan, Zexian Ding, Qi Yi

“…To study the binding mechanism between ATRX and heterochromatin protein 1 (HP1), both full-length and truncated mutants of hemagglutinin (HA)-ATRX were generated for co-immunoprecipitation and glutathione S-transferase (GST)-pull assays. …”
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Precision oncology through next generation sequencing in hepatocellular carcinoma by Sayali Shinde, Carola Maria Bigogno, Ana Simmons, Nikita Kathuria, Aruni Ghose, Vedika Apte, Patricia Lapitan, Shania Makker, Aydin Caglayan, Stergios Boussios

“…The knowledge gained from sequencing is then utilised to develop countermeasures against these mutants through different combination therapies. Advances in NGS have led to sequencing with higher accuracy and throughput, thereby enabling personalized and effective treatments. …”
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Metal Complexes and Their Potential Therapeutic Role Against COVID-19: Recent Developments in Drug Designing by Kanwal Ashiq, Bushra Naureen, Sana Ashiq

“…Despite the development of various vaccines, there is still an urgent need to design novel treatments backed with safety data for fighting  SARS-CoV-2 and its various mutants. Currently, scientists are putting their strenuous efforts into finding the best treatment option for COVID-19. …”
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Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action by Keerang Park, Michael E. Pape, Ki-Han Kim

“…By using 5' deletion mutants of promoter II fused to the bacterial chloramphenicol acetyltransferase (CAT) gene, the DNA mobility shift assay and the DNase I footprinting assay, the authors have identified the 30 bp from −389 to −359 as the TNF responsive element in promoter II. …”
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Sensory regulation of meal sorting in Aedes aegypti mosquitoes by Emi Maekawa, Anupama A. Dahanukar

“…Additionally, the robustness of meal sorting declines with mosquito age and is compromised in mutants of candidate thermoreceptors. Proper blood meal sorting is crucial for optimal egg production, as disruption of this process by adding sucrose results in reduced fecundity. …”
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Gene Therapy for HIV Infections: Intracellular Immunization by Alain Piché

“…These strategies include the use of single-chain antibodies, capsid-targeted viral inactivation, transdominant negative mutants, ribozymes, antisense oligonucleotides and RNA decoys. …”
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Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis by Karen Hudson

“…These lines are likely to represent 52 distinct genetic mutations. These mutants may represent new loci involved in the determination of soybean seed oil content or could be new isolates or alleles of previously identified genetic variants for soybean oil composition.…”
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Engineering artificial photosynthetic life-forms through endosymbiosis by Jay Cournoyer, Sarah D. Altman, Yang-le Gao, Catherine L. Wallace, Dianwen Zhang, Guo-Hsuen Lo, Noah T. Haskin, Angad P. Mehta

“…Here, we design and engineer artificial, genetically tractable, photosynthetic endosymbiosis between photosynthetic cyanobacteria and budding yeasts. We engineer various mutants of model photosynthetic cyanobacteria as endosymbionts within yeast cells where, the engineered cyanobacteria perform bioenergetic functions to support the growth of yeast cells under defined photosynthetic conditions. …”
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Importance of Lipopolysaccharide and Cyclic β-1,2-Glucans in Brucella-Mammalian Infections by Andreas F. Haag, Kamila K. Myka, Markus F. F. Arnold, Paola Caro-Hernández, Gail P. Ferguson

“…This paper will focus on the role of LPS and cyclic β-1,2-glucans in Brucella-mammalian infections and discuss the use of mutants, within the biosynthesis pathway of these cell envelope structures, in vaccine development.…”
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Irradiation-induced Deinococcus radiodurans genome fragmentation triggers transposition of a single resident insertion sequence. by Cécile Pasternak, Bao Ton-Hoang, Geneviève Coste, Adriana Bailone, Michael Chandler, Suzanne Sommer

“…The majority of thyA mutants resulted from transposition of one particular Insertion Sequence (IS), ISDra2, of the many different ISs in the D. radiodurans genome. …”
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Dual role of ramR mutation in enhancing immune activation and elevating eravacycline resistance in Klebsiella pneumoniae by Wei Yu, Peiyao Jia, Xiaobing Chu, Shengjie Li, Xinmiao Jia, Ying Zhu, Xiaoyu Liu, Yingchun Xu, Qiwen Yang

“…In mouse models, ramR L58P and F165L mutants showed lower bacterial burden in organs (spleen, lung, and kidney) 6 h post‐infection, and are fast cleared in 48 h. …”
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