Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening by Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun

“…Notably, six of the newly identified mutants exhibited similarities to the most effective mutants reported to date, indicating the promising application potential of the SerRF104I-based biosensor. …”
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Substantia nigra alterations in mice modeling Parkinson’s disease by I. N. Rozhkova, S. V. Okotrub, E. Yu. Brusentsev, T. A. Rakhmanova, D. A. Lebedeva, V. S. Kozeneva, N. A. Shavshaeva, N. V. Khotskin, S. Ya. Amstislavsky

“…Thus, the mice of the B6.Cg-Tg strain at the age of six months have some symptoms of the onset of PD, such as the accumulation of mutant alpha-synuclein and a decrease in the number of dopaminergic neurons in the substantia nigra. …”
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Tgfbr1 regulates lateral plate mesoderm and endoderm reorganization during the trunk to tail transition by Anastasiia Lozovska, Ana Casaca, Ana Novoa, Ying-Yi Kuo, Arnon D Jurberg, Gabriel G Martins, Anna-Katerina Hadjantonakis, Moises Mallo

“…The vascular remodeling involving the dorsal aorta and the umbilical artery leading to the connection between embryonic and extraembryonic circulation was also affected in the Tgfbr1 mutant embryos. Similar alterations in the LPM and vascular system were also observed in Isl1 null mutants, indicating that this factor acts in the regulatory cascade downstream of Tgfbr1 in LPM-derived tissues. …”
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UNC-10/SYD-2 links kinesin-3 to RAB-3-containing vesicles in the absence of the motor's PH domain by Odvogmed Bayansan, Prerana Bhan, Chien-Yu Chang, Syed Nooruzuha Barmaver, Che-Piao Shen, Oliver Ingvar Wagner

“…Similarly, motility of RAB-3-labeled vesicles is only slightly altered in nematodes carrying a point mutation in the PH domain, whereas movement of SNB-1 is significantly reduced in this mutant. Western blots from purified fractions of synaptic vesicles reveal strong reduction of UNC-104 in rab-3/unc-10 double mutants. …”
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Elite Alleles of EPE1 Identified via Genome-wide Association Studies Increase Panicle Elongation Length in Rice by Hao Sun, Qiqi Yao, Mei Hai, Tianhu Li, Jinghan Sun, Zhengbo Liu, Yang Ang, Yingying Zhao, Yanan Zhang, Xianping Cheng, Tao Huang, Yinping Chang, Mingyu Du, Erbao Liu

“…Compared with the short PELs of wild plants, mutant plants with Cas9-induced knockout (KO) of OsGA2ox9 exhibit long PELs. …”
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Drosophila ubiquitin-specific peptidase 14 stabilizes the PERIOD protein by regulating a ubiquitin ligase SLIMB by So Who Kang, Jung-Eun Park, Soonhyuck Ok, Minhui Um, Hyeonjeong Son, Seunghee Byun, Nayoung Park, Su Jin Lee, Thị Xuân Thùy Trần, Gyeongmin Kim, Jeonghun Yeom, Kyunggon Kim, Eun Young Kim, Min-Ji Kang

“…Expression of PER1117A, 1118A mutant in per 01 background impairs circadian rhythm strength. …”
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N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis by Mariana O. Ribeiro, Mafalda Oliveira, Verónica Nogueira, Vítor Costa, Vitor Teixeira

“…Methods Quantitative untargeted mass spectrometric proteomic and lipidomic analyses were conducted to examine changes in protein and lipid abundance in wild-type (WT) versus N88S seipin-expressing mutant cells. Differentially expressed proteins were categorized into functional networks to highlight altered protein functions and signaling pathways. …”
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Genome-wide identification of short-chain dehydrogenases/reductases genes and functional characterization of ApSDR53C2 in melanin biosynthesis in Arthrinium phaeospermum by Jiao Liao, Yisi Wang, Han Liu, Sijia Liu, Peng Yan, Hang Chen, Shujiang Li, Shujiang Li

“…Candidate ApSDRs screened by transcriptome sequencing were compared by qPCR experiments to obtain key ApSDRs that may play an important role in infestation and adversity resistance. Knockout mutants, the co-knockout mutant, and backfill mutants of key ApSDRs were obtained for phenotypic and stress conditions analysis. …”
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The discovery and characterization of K‐563, a novel inhibitor of the Keap1/Nrf2 pathway produced by Streptomyces sp by Ran Hori, Kozo Yamaguchi, Hidetaka Sato, Miwa Watanabe, Kyoko Tsutsumi, Susumu Iwamoto, Masayuki Abe, Hideyuki Onodera, Satoshi Nakamura, Ryuichiro Nakai

“…To discover a small‐molecule Keap1/Nrf2 pathway inhibitor, we conducted high‐throughput screening in Keap1 mutant human lung cancer A549 cells using a transcriptional reporter assay. …”
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Characterizing immune biomarkers and effector CD8+ T-cell exhaustion in pancreatic adenocarcinoma via single-cell RNA sequencing profiling by Rawaa AlChalabi, Raghda Makia, Semaa A. Shaban, Ahmed AbdulJabbar Suleiman

“…Drug repurposing of oxaprozin and celecoxib showed favorable binding interactions with both wild and mutant IL7R proteins. Conclusions: The K110N mutation, despite not causing significant structural changes, may impact T-cell and B-cell homeostasis and development. …”
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Neuronal Ceroid Lipofuscinosis in Border Collie Dogs in Japan: Clinical and Molecular Epidemiological Study (2000–2011) by Keijiro Mizukami, Takuji Kawamichi, Hiroshi Koie, Shinji Tamura, Satoru Matsunaga, Shigeki Imamoto, Miyoko Saito, Daisuke Hasegawa, Naoaki Matsuki, Satoshi Tamahara, Shigenobu Sato, Akira Yabuki, Hye-Sook Chang, Osamu Yamato

“…The genotyping survey demonstrated a high mutant allele frequency in examined kennels (34.8%). …”
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Ductal Carcinoma In Situ (DCIS) Diagnosed by MRI-Guided Biopsy among BRCA1/BRCA2 Mutation Carriers by Renata Faermann, Eitan Friedman, Orit Kaidar-Person, Jonathan Weidenfeld, Malka Brodsky, Anat Shalmon, Osnat Halshtok Neiman, Michael Gotlieb, Yael Yagil, David Samoocha, Dana Madorsky Feldman, Miri Sklair-Levy

“…While BRCA1/BRCA2 pathogenic sequence variants (PSVs) clearly confer an increased risk for invasive breast cancer, the extent to which these mutant alleles increase DCIS risk is less clear. Objective. …”
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Clinical role of endothelial dysfunction and hemostasis gene allelic variants in hepatitis C virus-associated cryoglobulinemia by M. V. Sokolova, Ye. Ye. Starostina, T. N. Krasnova, L. M. Samokhodskaya, T. P. Rozina, V. G. Avdeyev, M. G. Artemova, Ye. B. Yarovaya, N. A. Mukhin

“…Multivariate assay revealed the number of mutant alleles («total score») of NOS3 894 G/T, ITGA2 807 C/T and ITGB3 1565 T/C genes as independent risk factor. …”
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Surgical Resection with Neoadjuvant Chemotherapy for Locoregionally Recurrent Appendiceal Cancer Invading the External Iliac Vessels by Jun Takahashi, Shingo Tsujinaka, Nao Kakizawa, Noriya Takayama, Erika Machida, Kazuki Iseya, Fumi Hasegawa, Rina Kikugawa, Yasuyuki Miyakura, Koichi Suzuki, Toshiki Rikiyama

“…The pathological evaluation revealed KRAS-mutant adenocarcinoma with the final stage of T4bN1M0. …”
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ERK1/2 mitogen‐activated protein kinase dimerization is essential for the regulation of cell motility by Dalia de laFuente‐Vivas, Vincenzo Cappitelli, Rocío García‐Gómez, Sara Valero‐Díaz, Camilla Amato, Javier Rodriguéz, Santiago Duro‐Sánchez, Alexander vonKriegsheim, Michael Grusch, José Lozano, Joaquín Arribas, Berta Casar, Piero Crespo

“…We also show that a constitutively dimeric ERK mutant can drive cell motility per se, demonstrating that ERK dimerization is both necessary and sufficient for inducing cellular migration. …”
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Functional characterization of endocytic signals in the SynDIG/PRRT family members SynDIG1 and SynDIG4 in heterologous cells and neurons by David J. Speca, Chun-Wei He, Christina M. Meyer, Erin C. Scott, Erin C. Scott, Elva Díaz

“…We also show that SynDIG4 mutants lacking an endocytic signal co-localize with GluA1 but less so with GluA2 on the surface of heterologous cells. …”
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Positively charged cytoplasmic residues in corin prevent signal peptidase cleavage and endoplasmic reticulum retention by Hui Li, Shijin Sun, Wenjun Guo, Lina Wang, Zihao Zhang, Yue Zhang, Ce Zhang, Meng Liu, Shengnan Zhang, Yayan Niu, Ningzheng Dong, Qingyu Wu

“…In signal peptidase-deficient cells, the mutant corin proteins were not secreted but retained in the ER. …”
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Effect of KRAS mutation status on clinicopathological characteristics and overall survival in patients with rectal cancer by Shengbin Zheng, Zhijie You, Guodon Guo, Zhijing Lin, Siming Wang, Guohua Yang

“…The patients were categorized into two groups according to their KRAS status: wild-type KRAS (wKRAS) and mutant KRAS (mKRAS), with performing research on mKRAS G12D and mKRAS G13D mutation. …”
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Functional and structural characterization of mouse Factor H-related B protein unveils a novel dimerization domain shared by FHR-B and FH by Adrián Martín-Ambrosio Doménech, Silvia González Sanz, Bárbara Márquez Tirado, Lucia Juana-López, Elena Goicoechea de Jorge, Santiago Rodríguez de Córdoba, Héctor Martín Merinero

“…Using purified plasma proteins and recombinant mutants, FHR-B was found to form dimers and bind strongly to C3, C3b, iC3b, and C3dg. …”
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Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus by Jiang Ye, Xiaowei Wu, Xiang Li, Yuting Zhang, Yuqing Li, Hui Zhang, Jie Chen, Yuyan Xiang, Yefan Xia, Hu Zhao, Zengdong Tan, Liang Guo, Xuan Yao

“…BnaA09.MYB52 activates the expression of PMEI14 and BAN. Mutant analyses indicate that the SOC is increased by 4.3%–7.7%, while seed coat thickness shows a 12.9%–22.8% reduction in the ban and pmei14 mutants. …”
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