SCN10A gene polymorphism is associated with pain sensitivity and postoperative analgesic effects in patients undergoing gynecological laparoscopy by Yang Gao, Jing Li, Lin Gan, Meng Cai, Xiaofeng Lei, Jin Yu

“…SCN10A rs6795970 mutations were identified using MassARRAY SNP typing technology, and patients were categoried into homozygous mutant (AA), wild type (GG), and heterozygous mutation (GA) groups for analysis. …”
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In silico evaluation of missense SNPs in cancer-associated Cystatin A protein and their potential to disrupt Cathepsin B interaction by Shafaat Hossain, Omar Hamza Bin Manjur, Mst Sharmin Sultana Shimu, Tamanna Sultana, Mustafizur Rahman Naim, Shahariar Siddique, Abdullah Al Mamun, Md Miftaur Rahman, Md Abu Saleh, Md Rakibul Hasan, Tania Rahman

“…The root mean square fluctuation (RMSF) of the E94K mutant showed insufficient flexibility, indicating a reduced capacity to suppress CTSB. …”
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…The regulatory relationship between miR-135a-5p and LINC00599 was verified by constructing miR-135a-5p mimics, miR-135a-5p inhibit, wild type LINC00599 3′-untranslated region (UTR), and mutant LINC00599 3′-UTR. Ki-67 expression in the tumor tissues of nude mice was detected using immunofluorescent assays. …”
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A novel strategy for the protective effect of ginsenoside Rg1 against ovarian reserve decline by the PINK1 pathway by Pengdi Yang, Meiling Fan, Ying Chen, Dan Yang, Lu Zhai, Baoyu Fu, Lili Zhang, Yanping Wang, Rui Ma, Liwei Sun

“…Protein expression levels in the PINK1/Parkin pathway were assessed, and molecular docking and PINK1 mutant analyses were conducted to identify potential targets.Results Ginsenoside Rg1 significantly mitigated ovarian reserve decline, enhancing offspring quantity and quality, increasing the levels of ecdysteroids, preventing ovarian atrophy, and elevating germline stem cell numbers in aged Drosophila. …”
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Development of Safe and Non-Self-Immunogenic Mucosal Adjuvant by Recombinant Fusion of Cholera Toxin A1 Subunit with Protein Transduction Domain by Byoung-Shik Shim, In Su Cheon, Eugene Lee, Sung-Moo Park, Youngjoo Choi, Dae-Im Jung, Eunji Yang, Jung-ah Choi, June Young Chun, Jae-Ouk Kim, Cheol-Heui Yun, Cecil Czerkinsky, Man Ki Song

“…Moreover, in vivo cytotoxic T lymphocyte activity elicited by TCTA1T was significantly higher than that elicited by a mutant TCTA1T (TmCTA1T) lacking ADP-ribosyltransferase function. …”
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A root system architecture regulator modulates OsPIN2 polar localization in rice by Yong Li, Meiyan Ren, Yunrong Wu, Lingling Wang, Keju Zhao, Hongsheng Gao, Mengzhen Li, Yu Liu, Jianshu Zhu, Jiming Xu, Xiaorong Mo, Zhongchang Wu, Chungui Lu, Shaojian Zheng, Chuanzao Mao

“…We cloned and characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 (OsGLS1), in rice (Oryza sativa L.). The gls1 mutant displays an increased root growth angle, longer primary roots, more adventitious roots and greater nutrient uptake efficiency and grain yield in paddy fields. …”
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Truncated NS1 Influenza A Virus Induces a Robust Antigen-Specific Tissue-Resident T-Cell Response and Promotes Inducible Bronchus-Associated Lymphoid Tissue Formation in Mice by Anna-Polina Shurygina, Marina Shuklina, Olga Ozhereleva, Ekaterina Romanovskaya-Romanko, Sofia Kovaleva, Andrej Egorov, Dmitry Lioznov, Marina Stukova

“…Background: Influenza viruses with truncated NS1 proteins show promise as viral vectors and candidates for mucosal universal influenza vaccines. These mutant NS1 viruses, which lack the N-terminal half of the NS1 protein (124 a.a.), are unable to antagonise the innate immune response. …”
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Metabolic Blockade-Based Genome Mining of <i>Malbranchea circinata</i> SDU050: Discovery of Diverse Secondary Metabolites by Hu Yang, Xiaowei Luo, Zhuo Shang, Kunlong Li, Jian Cai, Yingying Chen, Longchao Xin, Jianhua Ju

“…This study reports the isolation and identification of four classes of secondary metabolites from wild-type <i>M. circinata</i> SDU050, alongside five additional metabolite classes, including three novel cytochalasins (<b>7</b>–<b>9</b>), obtained from a mutant strain through the metabolic blockade strategy. …”
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Unraveling the interactive effect of opaque2 and waxy1 genes on kernel nutritional qualities and physical properties in maize (Zea mays L.) by Subhra J. Mishra, Ikkurti Gopinath, Vignesh Muthusamy, Rajkumar U. Zunjare, Gulab Chand, Arun K. T. Venkatesh, Elangbam L. Devi, Konsam Sarika, Zahirul A. Talukder, Jitender Kumar, Firoz Hossain

“…Abstract The mutant waxy allele (wx1) is responsible for increased amylopectin in maize starch, with a wide range of food and industrial applications. …”
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ApWD40a, a Member of the WD40-Repeat Protein Family, Is Crucial for Fungal Development, Toxin Synthesis, and Pathogenicity in the Ginseng Alternaria Leaf Blight Fungus <i>Alternari... by Jinling Lan, Shengjie Mei, Yingxue Du, Meili Chi, Jiayi Yang, Shuliu Guo, Mingliang Chu, Ronglin He, Jie Gao

“…The deletion of <i>ApWD40a</i> impaired the mycelial growth, reduced the sporulation, and significantly decreased the efficiency in utilizing various carbon sources. The Δ<i>Apwd40a</i> mutant showed increased sensitivity to osmotic stress and metal ion stress induced by sorbitol, NaCl, and KCl, but decreased the sensitivity to a cell wall stress factor (SDS) and oxidative stress factors (paraquat and H₂O₂). …”
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Effects of Clinicopathological Characteristics on the Survival of Patients Treated with PD-1/PD-L1 Inhibitor Monotherapy or Combination Therapy for Advanced Cancer: A Systemic Revi... by Yuhan Wei, Yongfu Li, Qi Du, Xinyi Peng, Jiangtao Jin, Hong Guo, Yongyan Li, Qin Li

“…EGFR wild-type patients could benefit from immunotherapy monotherapy (HR, 0.77; P<0.001) while those of the mutant type had no survival benefit (HR, 1.11; P=0.54), and the difference was statistically significant (interaction, P=0.005). …”
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Impact of meropenem exposure on fluoroquinolone and carbapenem resistance in Pseudomonas aeruginosa infection in inpatients in a Japanese university hospital: Insights into oprD mu... by Tadanori Yamochi, Kazuhisa Ugajin, Rintaro On, Sho Inoue, Hiromi Ikeda, Toshiko Yamochi, Masafumi Takimoto, Issei Tokimatsu

“…Meropenem exposure within the past 59 days may have contributed to the selection of the oprD mutant overexpressing mexB, and meropenem exposure within the past 6 months may have contributed to meropenem resistance.…”
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Abnormal cytoskeletal remodeling but normal neuronal excitability in a mouse model of the recurrent developmental and epileptic encephalopathy-susceptibility KCNB1-p.R312H variant by Alessandro Bortolami, Elena Forzisi Kathera-Ibarra, Anastasia Balatsky, Mansi Dubey, Rusheel Amin, Srinidi Venkateswaran, Stefania Dutto, Ishan Seth, Adam Ashor, Angel Nwandiko, Ping-Yue Pan, David P. Crockett, Federico Sesti

“…Surprisingly, the electrical properties of Kcnb1 R312H(+/+) pyramidal neurons are similar to those of WT neurons, indicating that the arginine to histidine replacement does not affect the conducting properties of the mutant channel. In contrast, fluorescence recovery after photobleaching, biochemistry, and immunofluorescence, reveal marked differences in the way WT and Kcnb1 R312H(+/+) neurons modulate the remodeling of the actin cytoskeleton, a key player in the processes underlying neurodevelopment. …”
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Human striatal progenitor cells that contain inducible safeguards and overexpress BDNF rescue Huntington’s disease phenotypes by Danielle A. Simmons, Sridhar Selvaraj, Tingshuo Chen, Gloria Cao, Talita Souto Camelo, Tyne L.M. McHugh, Selena Gonzalez, Renata M. Martin, Juste Simanauskaite, Nobuko Uchida, Matthew H. Porteus, Frank M. Longo

“…Engrafted STRpcs overexpressing BDNF alleviated motor and cognitive deficits and reduced mutant huntingtin aggregates. Activating the inducible safety switch with rapamycin safely eliminated the engrafted cells. …”
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High glucose induces podocyte ferroptosis through BAP1/SLC7A11 pathway by Ren Peiyao, Man Xueli, Sun Wenbo, Zheng Danna, Gong Jianguang, Jin Juan, He Qiang

“…In BAP1 WT cells, but not in its C91A mutant cells, the transcription of SLC7A11 was downregulated and the level of ferroptosis was increased. …”
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Marigold: a machine learning-based web app for zebrafish pose tracking by Gregory Teicher, R. Madison Riffe, Wayne Barnaby, Gabrielle Martin, Benjamin E. Clayton, Josef G. Trapani, Gerald B. Downes

“…First, we examined novel aspects of the touch-evoked escape response in techno trousers (tnt) mutant embryos, which contain a previously described loss-of-function mutation in the gene encoding Eaat2b, a glial glutamate transporter. …”
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Phage vB_Kpn_HF0522: Isolation, Characterization, and Therapeutic Potential in Combatting K1 Klebsiella pneumoniae Infections by Yan T, Wang Q, Ma C, Teng X, Gong Z, Chu W, Zhou Q, Liu Z

“…The bacterial growth rate remained largely unchanged after the phage resistance mutation, but mice infected with the mutant strain showed significantly higher survival rates than those infected with the wild-type strain. vB_Kpn_HF0522 increased the survival rate of infected G. mellonella from 12.5% to 75%, inhibited incisional surgical site infections and alleviated inflammatory response in mice.Conclusion: These findings indicate that vB_Kpn_HF0522 has significant potential for treating specific bacterial infections, and may serve as an antimicrobial agent for research and clinical anti-infective therapy.Keywords: phage vB_Kpn_HF0522, phage therapy, Klebsiella pneumoniae, phage resistance, incisional surgical site infection, biofilm…”
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Pyrrocidines A and B demonstrate synergistic inhibition of Fusarium verticillioides growth by Lily W. Lofton, Lily W. Lofton, Quentin D. Read, Hailey L. Hamilton, Anthony E. Glenn, Jaci A. Hawkins, Trevor R. Mitchell, Scott E. Gold

“…Additionally, we showed that the FvABC3 (FVEG_11089) mutant, earlier described as hypersensitive to pyrrocidine, is particularly sensitive to pyrrocidine B. …”
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Importance of benzoyltransferase GcnE and lysine benzoylation of alcohol dehydrogenase AdhB in pathogenesis and aflatoxin production in Aspergillus flavus by Xuan Chen, Lihan Wu, Yuqi Zhang, Sen Wang, Shihua Wang

“…Our data demonstrated that alcohol dehydrogenase B (AdhB) is regulated by benzoylation on lysine 321 (K321), and mutations of Kbz site in AdhB significantly reduced the alcohol dehydrogenase activity in vivo and in vitro. Both adhB deletion mutant and benzoylated site mutants (K321R and K321A) exhibited similar phenotype, including decreased conidiation and seed colonization, increased sclerotia formation and aflatoxin production, and more sensitive to cell wall damage stress. …”
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Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan by Kaori Mukai, Robert Cost, Xin Sheen Zhang, Emily Condiff, Joanne Cotton, Xiaohua Liu, Ekaterina Boudanova, Björn Niebel, Peter Piepenhagen, Xinming Cai, Anna Park, Qun Zhou

“…The method uses a high affinity synthetic M6P glycan, bisM6P, that is coupled to an Fc-engineered antibody NNAS. This mutant without any effector function was generated by switching the native glycosylation site from position 297 to 298 converting non-sialylated structures to highly sialylated N-glycans. …”
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