Preliminary analysis of the role of small hepatitis B surface proteins mutations in the pathogenesis of occult hepatitis B infection via the endoplasmic reticulum stress-induced UP... by Huang Chengrong, Zhang Hao, Wang Jing, Li Jianfei, Liu Qian, Zong Qiyin, Zhang Yunyun, Wang Qin, Zhou Qiang

“…Intracellular and extracellular HBsAg levels in these mutant plasmid-transfected cells were lower relative to those for WT plasmid-transfected cells, and HBsAg accumulation within the ER was detected via immunofluorescent staining in cells transfected with OBI-related SHB mutation-encoding plasmids, ERS-related protein content was also significantly increased in mutant plasmid-transfected cells as compared to those in the WT group. …”
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SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation by Ryan T. Wagner, Ryan A. Hlady, Xiaoyu Pan, Liguo Wang, Sungho Kim, Xia Zhao, Louis Y. El Khoury, Shafiq Shaikh, Jian Zhong, Jeong-Heon Lee, Jolanta Grembecka, Tomasz Cierpicki, Thai H. Ho, Keith D. Robertson

“…Depletion of NSD1 using a CRISPRi targeting approach promotes the loss of SETD2-mutant cells coincident with elevated levels of DNA damage and apoptosis. …”
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Low-exhaustion peripheral circulating γδ T cells serve as a biomarker for predicting the clinical benefit rate of non-small cell lung cancer (NSCLC) patients to chemotherapy or tar... by Dongdong Zhang, Guichao Liu, Jinhui Ye, Ke Li, Guojun Zhang, Qiang Quan, Xinhai Zhu, Peng Li

“…Methods Patients with EGFR wild-type (EGFR-WT) or mutant (EGFR-Mut) non-small cell lung cancer (NSCLC), diagnosed between January 2020 and January 2024, were included in this study. …”
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Comparison of Efficacy and Safety of First-Line Treatment Options for Unresectable Stage III Non-Small Cell Lung Cancer: A Retrospective Analysis by Luqing Zhao, Zhiting Zhao, Xiaoqi Yan, Fei Wu, Ning Sun, Renhong Guo, Shaorong Yu, Xiao Hu, Jifeng Feng

“…For unresectable stage III NSCLC patients with wild-type driver genes, the combination of radiotherapy and immunotherapy in the initial treatment was essential to significantly improve the efficacy. For patients with mutant driver genes, radiotherapy, targeted therapy, and the combination of radiotherapy and targeted therapy showed similar short-term efficacy.…”
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Unveiling immunogenic characteristics and neoantigens in endometrial cancer with POLE hotspot mutations for improved immunotherapy by Jian Huang, Shuangna Song, Yihua Yin, Yinyan He, Huimin Wang, Ye Gu, Laman He, Xintao Wang, Xiaocao Miao, Zhigang Zhang, Xueli Zhang, Yiran Li, Yiran Li

“…Consequently, by constructing human leukocyte antigen (HLA) tetramers and incubating them with patients’ peripheral blood mononuclear cells (PBMCs), T cells capable of recognizing the POLEP286R mutation were sorted for further transcriptomic, proteomic and T-cell receptor (TCR) sequencing analyses and for an organoid EC model.ResultsTumor- and immune-related pathways were shown to be activated in the POLEP286R mutant group. Importantly, by using an organoid model of EC, we further confirmed the antitumor potential of T cells that were specific to the POLEP286R mutation.ConclusionsOur study uncovers the pronounced immunogenicity of POLE-mutant EC and characterizes neoantigens that are unique to the POLEP286R mutation, thus providing a promising new immunotherapeutic strategy for EC.…”
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Antimony Resistance in Leishmania, Focusing on Experimental Research by Fakhri Jeddi, Renaud Piarroux, Charles Mary

“…Finally, we detail molecular mechanisms and targets involved in resistance and already identified in vitro within selected mutant strains or in clinical isolates.…”
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Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon by Sylvia A. Reimann, Alan J. Wolfe

“…In this study, we show that the E. coli ompR malTcon double mutant exhibits a synthetic lethal phenotype that is environmentally conditional. …”
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Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium by Agnieszka Michael, Justin John, Brendan Meyer, Hardev Pandha

“…We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). …”
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Syndrome of Reduced Sensitivity to Thyroid Hormones: Two Case Reports and a Literature Review by Anastasios Anyfantakis, Dimitrios Anyfantakis, Irene Vourliotaki

“…Most patients with RTH have mutations in the gene that encodes the β isoform of the receptor of thyroid hormone (THR-β gene). Mutant receptors are unable to activate or repress target genes. …”
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Effect of ADH II Deficiency on the Intracellular Redox Homeostasis in Zymomonas mobilis by Nina Galinina, Zane Lasa, Inese Strazdina, Reinis Rutkis, Uldis Kalnenieks

“…Mutant strain of the facultatively anaerobic, ethanol-producing bacterium Zymomonas mobilis, deficient in the Fe-containing alcohol dehydrogenase isoenzyme (ADH II), showed impaired homeostasis of the intracellular NAD(P)H during transition from anaerobic to aerobic conditions, and also in steady-state continuous cultures at various oxygen supplies. …”
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A mutation in RNA polymerase imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism by Yesha Patel, John D. Helmann

“…The CEF-resistant rpoC G1122D mutant evades these metabolic perturbations, and repression of the BCAA and pyrimidine pathways may function to restrict membrane biogenesis, which is beneficial when cell wall synthesis is impaired. …”
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A Faster Convergence Artificial Bee Colony Algorithm in Sensor Deployment for Wireless Sensor Networks by Xiangyu Yu, Jiaxin Zhang, Jiaru Fan, Tao Zhang

“…Some new parameters such as forgetting and neighbor factor for accelerating the convergence speed and probability of mutant for maximizing the coverage rate are introduced. …”
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Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method by Rajagopalan Prabu, Thomas Chand, Sunhare Raksha

“…A. niger was mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mutants (A. niger UV-112) was capable of producing sodium gluconate up to final concentrations of 60 g/L in batch fermentation, which was 3.0 fold higher than the parent strain. …”
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Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL) by Marcus Wäneskog, Emma Elise Hoch-Schneider, Shilpa Garg, Christian Kronborg Cantalapiedra, Elena Schäfer, Michael Krogh Jensen, Emil Damgaard Jensen

“…ABSTRACT High-throughput DNA transformation techniques are invaluable when generating high-diversity mutant libraries, a cornerstone of successful protein engineering. …”
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HDC1 Promotes Primary Root Elongation by Regulating Auxin and K⁺ Homeostasis in Response to Low-K⁺ Stress by Xiaofang Kuang, Hao Chen, Jing Xiang, Juan Zeng, Qing Liu, Yi Su, Chao Huang, Ruozhong Wang, Wanhuang Lin, Zhigang Huang

“…Using a <i>hdc1-2</i> mutant line, we observed that HDC1 positively regulated root growth under LK conditions. …”
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CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice by Yu Shicong, Luo Ruxian, Zheng Shuqin, Ning Jing, Shi Yuanzhu, Guo Daiming, Jia Liangmeng, Wang Sen, Xiao Guizong, Guo Pengwang, Li Yang, Ma Xiaoding

“…From an ethyl methanesulfonate (EMS)-mutagenized mutant library, we identified a mutant that is susceptible to osmotic stress, named Osmotic Stress Sensitivity 1 (Oss1). …”
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An Efficient Method for Immortalizing Mouse Embryonic Fibroblasts by CRISPR-mediated Deletion of the Tp53 Gene by Srisathya Srinivasan, Hsin-Yi Ho

“…The MEF system can also be combined with rescue studies to characterize the function of mutant genes/proteins, such as disease-causing variants. …”
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Molecular profiling and detection methods of microRNA in cancer research by Nurul-Syakima Ab Mutalib, Imilia Ismail, Hooi-Leng Ser

“…In truth, the first discovery of miRNA was in a simple organism -nematode (scientific name: Caenorhabditis elegans), whereby a mutant displayed aberrant morphological changes. Years after that, researchers then realized that these miRNAs are actually important regulatory molecules — controlling cell division signaling, apoptosis and so on. …”
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Do strigolactones play a role in the ascent and attachment behavior of Pisum sativum? by Bianca Bonato, Tom Bennett, Silvia Guerra, Sara Avesani, Umberto Castiello

“…To assess this, we compare the typical behavior of P. sativum, a wild-type plant that produces and perceives SLs, with mutants defective in SLs synthesis or signaling, known as ramosus(rms) mutants. …”
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University of Florida Potato Variety Trials Spotlight: Atlantic by Mario H. M. L. Andrade, Rodrick Z. Mwatuwa, Christian T. Christensen, Lincoln Zotarelli

“…The cultivar was released as a white mutant of the USDA breeding program. It was selected from a cross of Wauseon and Lenape (UDSA seedling B5141-6). …”
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