Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy by Apisitt Thaiprayoon, Yodpong Chantarasorn, Worrapoj Oonanant, Anongnard Kasorn, Phoomintara Longsompurana, Satita Tapaneeyakorn, Pinpunya Riangrungroj, Fabien Loison, Andrew C. Kruse, Matthew P. DeLisa, Dujduan Waraho-Zhmayev

“…This combined process enabled isolation of three unique Nb clones (NbT15, NbT21, and NbT22) that all bound specifically to a target antigen, namely proprotein convertase subtilisin/kexin type 9 (PCSK9) as well as a gain-of-function PCSK9 mutant (D374Y). All three clones bound to PCSK9 and blocked the interaction between the low-density lipoprotein receptor (LDLR) and either wild-type PCSK9 or the D374Y mutant. …”
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Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families in Aspergillus niger highlighting the versatility of the fu... by Katharina J. Ost, Mark Arentshorst, Bruno M. Moerschbacher, Mareike E. Dirks-Hofmeister, Arthur F.J. Ram

“…The 12-fold deletion mutant was viable and did not exhibit growth defects under non-stressing growth conditions. …”
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Effect of RAS mutations and related immune characteristics on the prognosis of patients with MSI-H/dMMR colorectal cancer by Yupeng Jiang, Yuyao Liu, Hong Huang, Tiantian Zhao, Zengyi Zhao, Yawen Gao

“…Results RAS-mutant MSI-H colorectal patients were more likely to have: (1) higher platelet values; (2) shorter disease-free survival (DFS); (3) lower infiltrated numbers of CD11c+ dendritic cells, CD4+ T lymphocytes, and CD8+ T lymphocytes, and higher infiltrated numbers of Foxp3+ Treg cells. …”
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<i>Weizmannia coagulans</i> BC99 Attenuates Oxidative Stress Induced by Acute Alcoholic Liver Injury via Nrf2/SKN-1 Pathway and Liver Metabolism Regulation by Ying Wu, Cheng Li, Yinyin Gao, Jie Zhang, Yao Dong, Lina Zhao, Yuwan Li, Shaobin Gu

“…The protective effects of BC99 were markedly diminished in the <i>skn-1</i> mutant (GR2245) and <i>daf-16</i> mutant (CF1038), further confirming the pivotal roles of SKN-1 and DAF-16 pathways in BC99-mediated antioxidant protection. …”
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A Systematic Review of the Prevalence and Diagnostic Workup of PIK3CA Mutations in HR+/HER2– Metastatic Breast Cancer by Elizabeth J. Anderson, Lea E. Mollon, Joni L. Dean, Terri L. Warholak, Ayal Aizer, Emma A. Platt, Derek H. Tang, Lisa E. Davis

“…Given the clinical benefit of the PI3Ki alpelisib in patients with PIK3CA mutant HR+/HER2– mBC, determination of tumor PIK3CA mutation status is of importance in managing patients with HR+/HER2– mBC. …”
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Polymorphism of renin — angiotensin system genes in progression of liver fibrosis in patients with chronic hepatitis C by O. V. Taratina, T. N. Krasnova, L. M. Samokhodskaya, T. N. Lopatkina, V. A. Tkachuk, N. A. Mukhin

“…No significant differences between groups in distribution of alternative alleles and genotypes of ATR1 gene on A1166C locus have been revealed.Conclusion. Carriage of mutant alleles of angiotensinogen gene on any of loci (G-6A or M235T) is the factor predicting more rapid progression of disease. …”
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Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations by Nele Niebrügge, Olga Trovato, Roman Praschberger, Andreas Lieb

“…However, a detailed analysis of basal/constitutive activity also revealed a gain-of-function phenotype for mutant M345R. For a more comprehensive investigation of heterotrimeric G-protein complex signaling, we investigated the impact of WT mutants in combination with (i) a specifically suggested assay, and (ii) the most abundantly expressed heterotrimeric G-protein complex combinations in WT receptor-enriched regions. …”
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The regulatory effects of (p)ppGpp and indole on cAMP synthesis in <i>Escherichia coli</i> cells by N. M. Kashevarova, E. A. Khaova, A. G. Tkachenko

“…At the same time, the (p)ppGpp0 mutant showed a decrease in maximum cAMP levels compared to the (p)ppGpp+ parent, which was the most pronounced in the medium with 22.2 mM glucose. …”
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Hypoxia-inducible factor 1α is required to establish the larval glycolytic program in Drosophila melanogaster by Yasaman Heidarian, Tess D. Fasteen, Liam Mungcal, Kasun Buddika, Nader H. Mahmoudzadeh, Travis Nemkov, Angelo D'Alessandro, Jason M. Tennessen

“…Results: Our studies reveal that sima mutants fail to activate aerobic glycolysis and die during larval development with metabolic phenotypes that mimic those displayed by dERR mutants. …”
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University of Florida Potato Variety Spotlight: Red LaSoda by Lincoln Zotarelli, Doug Gergela, Chad M. Hutchinson, David Dinkins, Edsel Redden

“…It was observed in 1949 as a deep red mutant of LaSoda in the Louisiana potato breeding program. …”
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Multiple Control Strategies for Prevention of Avian Influenza Pandemic by Roman Ullah, Gul Zaman, Saeed Islam

“…To do this, we use three control functions, the effort to reduce the number of contacts with human infected with mutant avian influenza, the antiviral treatment of infected individuals, and the effort to reduce the number of infected birds. …”
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University of Florida Potato Variety Spotlight: Red LaSoda by Lincoln Zotarelli, Doug Gergela, Chad M. Hutchinson, David Dinkins, Edsel Redden

“…It was observed in 1949 as a deep red mutant of LaSoda in the Louisiana potato breeding program. …”
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Chinese Medicine Treatment for Afatinib-Induced Paronychia by Pei-Yuu Yang, Chen-Jei Tai

“…Afatinib (Gilotrif™) is widely used to treat patients with mutant activating epidermal growth factor receptor- (EGFR-) dependent lung adenocarcinoma; however, it has various adverse side effects. …”
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The inheritance pattern for the dwarf phenotype in hybrids from crosses among sunflower lines differing in alleles of the <i>Rht1</i> locus by I. N. Anisimova, G. V. Khafizova, L. G. Makarova, N. V. Alpatieva, M. K. Ryazanova, O. M. Borisenko, V. A. Gavrilova

“…Using the marker, the mutant Rht1 allele was identified in the VIR 171 and VIR 434 dwarf lines, similar in their origin and phenotype. …”
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Characterization of the Vibrio anguillarum VaRyhB regulon and role in pathogenesis by Yingjie Li, Xinran Yu, Peng Li, Xin Li, Lushan Wang

“…The sensitivity toward H2O2 in the ΔVafur mutant could be restored by the loss of VaRyhB, suggesting that the role of Fur in oxidative stress is mediated by VaRyhB. …”
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RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection by Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Fuminori Sugihara, Yasuhiko Horiguchi

“…Mouse infection experiments also showed that the hppD-deficient mutant was eliminated from the respiratory tract more rapidly than the wild-type strain, although the initial colonization levels were comparable between the two strains. …”
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Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies by Daniel F. Paulo, Thu N. M. Nguyen, Chris M. Ward, Renee L. Corpuz, Angela N. Kauwe, Pedro Rendon, Rocio E. Y. Ruano, Amanda A. S. Cardoso, Georgia Gouvi, Elisabeth Fung, Peter Crisp, Anzu Okada, Amanda Choo, Christian Stauffer, Kostas Bourtzis, Sheina B. Sim, Simon W. Baxter, Scott M. Geib

“…In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. …”
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Nucleic Acid-Based Therapy Approaches for Huntington's Disease by Tatyana Vagner, Deborah Young, Alexandre Mouravlev

“…These approaches include increasing expression of growth factors, decreasing levels of mutant huntingtin, and restoring cell metabolism and transcriptional balance. …”
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PU.1 and Haematopoietic Cell Fate: Dosage Matters by Ka Sin Mak, Alister P. W. Funnell, Richard C. M. Pearson, Merlin Crossley

“…This paper will discuss how a range of experiments in cell lines and mutant and transgenic mouse models have enhanced our knowledge of the mechanisms by which PU.1 drives lineage-specific differentiation during haematopoiesis.…”
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Mouse Models for Blistering Skin Disorders by Radhika Ganeshan, Jiangli Chen, Peter J. Koch

“…A comparison of the phenotypes observed in these mouse lines is provided and the relationship between the mutant mouse phenotypes and human diseases, in particular pemphigus vulgaris, is discussed. …”
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