Very first real-world data on zongertinib use in non-small cell lung cancer patients with HER2 mutations: A brief report by Oliver Illini, Anna Sophie Lang-Stöberl, Hannah Fabikan, Christoph Weinlinger, Arschang Valipour, Maximilian J. Hochmair

“…Introduction: Treatment options for HER2-mutant patients with non-small cell lung cancer (NSCLC) are limited. …”
Get full text

Peptide nucleic acid-immobilised paper combined with multiplex recombinase polymerase amplification for the ultrasensitive and rapid detection of rifampicin-resistant tuberculosis by Nuttapon Jirakittiwut, Panuwat Sathianpitayakul, Pitak Santanirand, Yukihiro Akeda, Tirayut Vilaivan, Panan Ratthawongjirakul

“…The absence of a signal was observed when the mutant strains were detected because of the inability to bind the mutant sequence. …”
Get full text

Dissection of Ire1 functions reveals stress response mechanisms uniquely evolved in Candida glabrata. by Taiga Miyazaki, Hironobu Nakayama, Yohsuke Nagayoshi, Hiroshi Kakeya, Shigeru Kohno

“…In this study, we comprehensively characterized mutant phenotypes of IRE1 and other related genes in the human fungal pathogen Candida glabrata. …”
Get full text

Combining Ability of Pod Yield and Related Traits of Groundnut (Arachis hypogaea L.) under Salinity Stress by Md. Abul Kalam Azad, Md. Shah-E-Alam, Md. Abdul Hamid, Mohd Y. Rafii, M. A. Malek

“…Cultivars “Binachinabadam-2” and “Dacca-1” and mutant M6/25/64-82 had the highest, second highest, and third highest pod number, as well as gca values, respectively. …”
Get full text

A NOTCH3 pathogenic variant influences osteogenesis and can be targeted by antisense oligonucleotides in induced pluripotent stem cells. by Ernesto Canalis, Jungeun Yu, Lauren Schilling, Paymaan Jafar-Nejad, Michele Carrer

“…ASOs targeting NOTCH3 decreased both NOTCH3 wild type and NOTCH36692-93insC mutant mRNA by 40% in mesenchymal and 90% in osteogenic cells. …”
Get full text

Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability by Guilong Wang, Zimin Liu, Li Xu, Yunjun Yan

“…By contrast, there are two amino acid residues (V94 and I100) with significant difference in the substrate binding pocket of YLLip2; they were subjected to site-directed mutagenesis (SDM) to introduce aromatic amino acid mutations. Two mutants (V94W and I100F) were created. The enzymatic properties of the mutant lipases were detected and compared with the wild-type. …”
Get full text

Study of the effect of the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro by E. G. Zakirova, Y. V. Vyatkin, N. A. Verechshagina, V. V. Muzyka, I. O. Mazunin, K. E. Orishchenko

“…It means that due to a high copy number of the mitochondrial genome, mutant copies of mtDNA coexist with wild-type molecules in the same organelle. …”
Get full text

Deletion of a Putative GPI-Anchored Protein-Encoding Gene Aog185 Impedes the Growth and Nematode-Trapping Efficiency of Arthrobotrys oligospora by Disrupting Transmembrane Transpor... by Hui Peng, Hengqian Lu, Xinyuan Dong, Xiao Liang, Kangliang Sheng, Jingmin Wang, Xiaowei Kong, Xiangdong Zha, Yongzhong Wang

“…Compared to the wild-type (WT) strain, the ΔAog185 mutant grew more slowly, exhibited a 20% decrease in conidiation, delayed conidial germination, generated fewer traps, attenuated nematode trapping efficiency, and was more sensitive to chemical stressors. …”
Get full text

Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium. by Iwona Bucior, Julia F Pielage, Joanne N Engel

“…In contrast, experiments utilizing a P. aeruginosa isogenic mutant that lacks Tfp or using beads coated with purified flagella demonstrate that flagella are necessary and sufficient for maximal binding to heparan sulfate (HS) chains of heparan sulfate proteoglycans (HSPGs) at the BL surface of polarized epithelium. …”
Get full text

Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. by Qin Zhou, Na Pu, Ge Xu, Hangchi Liu, Xudong Jia, Xiaomin Wang, Peng Xu

“…There was a significant difference in the MIC of the gyrA mutant types to FQs. The highest resistance to FQs was observed in the Gly88Cys mutant strains. …”
Get full text

Epigenetic regulation of the nuclear genome associated with mitochondrial dysfunction in Leber’s hereditary optic neuropathy (LHON) by Aswathy P. Nair, Ambika Selvakumar, Janani Gopalarethinam, B. Abishek Kumar, Balachandar Vellingiri, Mohana Devi Subramaniam

“…In comparison to their controls, both LHON patients and ND4 mutant cells exhibited a significant enrichment in activation and repressive markers. …”
Get full text

A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization in Clostridium Pasteurianum by Tom Nguyen, Luca W. G. Meleski, Minu P. Belavatta, Sivasubramanian Gurumoorthi, Chijian Zhang, Anna‐Lena Heins, An‐Ping Zeng

“…Additionally, examination of the phenotype of the generated mutant strain led to discovery of 2,3‐butanediol (2,3‐BDO) production of up to 0.48 g L−1, and this metabolite was not reported to be produced by C. pasteurianum before. …”
Get full text

Extramacrochaetae regulates Notch signaling in the Drosophila eye through non-apoptotic caspase activity by Sudershana Nair, Nicholas E Baker

“…Growth inhibition of emc mutant clones in wing imaginal discs also depended on caspases. …”
Get full text

Inference of a three-gene network underpinning epidermal stem cell development in Caenorhabditis elegans by Alicja Brożek, Arianna Ceccarelli, Andreas Christ Sølvsten Jørgensen, Mark Hintze, Vahid Shahrezaei, Michalis Barkoulas

“…We use single-molecule FISH to quantify TF mRNA levels in single seam cells of wild-type and mutant backgrounds across four early larval stages. …”
Get full text

Somatic Mutaome Profile in Human Cancer Tissues by Nayoung Kim, Yourae Hong, Doyoung Kwon, Sukjoon Yoon

“…In the present study, we integrated and analyzed the exome sequencing data from ~3,000 tissue samples and summarized the major mutant genes in each of the diverse cancer subtypes and stages. …”
Get full text

POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC by Ryana Budi Purnama, Setiadi Warata Logamarta, Agung Dhartono

“…From data analysis, X2 is 0,44 with p=0,50 (p>0,05) resulted. It was showing mutant VDR gene allele frequency in chronic periodontitis patients in Javanese Banyumas ethnic were balanced genetically. …”
Get full text

Marker-assisted identification of maize genotypes with improved protein quality by O. A. Orlovskaya, S. V. Kubrak, S. I. Vakula, L. V. Khotyleva, A. V. Kilchevsky

“…UREA-PAG electrophoresis of zein proteins was used for Quality Protein Maize (QPM) identification. In addition to the mutant o2 allele, QPM contains genetic modifiers that convert starchy endosperm of o2 mutant to the hard vitreous phenotype. …”
Get full text

Roles of the rpoEc-chrR-chrA operon in superoxide tolerance and β-lactam susceptibility of Stenotrophomonas maltophilia by Ren-Hsuan Ku, Hsu-Feng Lu, Li-Hua Li, Li-Hua Li, Ting-Yu Yeh, Yi-Tsung Lin, Yi-Tsung Lin, Tsuey-Ching Yang

“…The contribution of rpoEc-chrR-chrA operon to oxidative stress alleviation and antibiotic susceptibility was evaluated using mutant constructs and stress-tolerance assays. RNA-seq transcriptome assay of wild-type KJ, KJΔChrR (chrR mutant), and KJΔChrRΔRpoEc (chrR/rpoEc double mutant) was performed to reveal the σEc regulon.ResultsThe rpoEc-chrR pair and downstream chrA formed an operon. …”
Get full text

Molecular Mechanisms of Synergistic Effect of PRIMA‐1met and Oxaliplatin in Colorectal Cancer With Different p53 Status by Xiao‐lan Li, Jianbiao Zhou, Nicole Xin‐Ning Tang, Yi Chai, Meng Zhou, Ai‐di Gao, Zhong‐kai Lu, Han Min

“…PRIMA‐1met (APR‐246, eprenetapopt) restores the DNA‐binding capacity of different mutant P53 proteins. PRIMA‐1met has progressed to the Phase III clinical trial. …”
Get full text

A rare deleterious missense mutation in the AXIN2 gene in Chinese women with polycystic ovary syndrome by Ying Zhang, Yu Chen, Fa-Ying Liu, Jing-Wen Fu, Xian-Xian Liu, Jun Tan, Ou-Ping Huang, Yang Zou

“…The evolutionary conservation analysis of the identified Axin-2 mutant was analyzed among 15 vertebrates from human to zebrafish. …”
Get full text