Immune responses in rodent whole eye transplantation: elucidation and preliminary investigations into rejection diagnosis and monitoring by Bing Li, Yong Wang, Charles R. Owens, Touka Banaee, Charleen T. Chu, Kayvon Jabbari, Anna D. Lee, Neil J. Khatter, Alan G. Palestine, An-Jey A. Su, Christene A. Huang, Kia M. Washington

“…These methodologies collectively contribute to the development of non-invasive diagnostic and monitoring strategies for WET.ResultsOur study revealed a rapid and acute immune response following WET, characterized by an early innate immune response dominated by complement involvement, and infiltration of neutrophils and monocytes by post-operative day (POD) 2. This was succeeded by an acute T-cell-mediated immune reaction, predominantly involving T helper 1 (Th1) cells and cytotoxic T lymphocytes (CTLs). …”
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Identification and validation of glycolysis-related diagnostic signatures in diabetic nephropathy: a study based on integrative machine learning and single-cell sequence by Xiaoyin Wu, Xiaoyin Wu, Buyu Guo, Buyu Guo, Xingyu Chang, Xingyu Chang, Yuxuan Yang, Yuxuan Yang, Qianqian Liu, Qianqian Liu, Jiahui Liu, Jiahui Liu, Yichen Yang, Yichen Yang, Kang Zhang, Yumei Ma, Songbo Fu, Songbo Fu, Songbo Fu

“…GScore was significantly associated with neutrophils and non-classical monocytes. Potential agents including esmolol, estradiol, ganciclovir, and felbamate, targeting the 12 diagnostic signatures were identified. …”
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Sickle Cell Anemia Patients Display an Intricate Cellular and Serum Biomarker Network Highlighted by TCD4+CD69+ Lymphocytes, IL-17/MIP-1β, IL-12/VEGF, and IL-10/IP-10 Axis by Nadja Pinto Garcia, Alexander Leonardo S. Júnior, Geyse Adriana S. Soares, Thainá Cristina C. Costa, Alicia Patrine C. dos Santos, Allyson Guimarães Costa, Andréa Monteiro Tarragô, Rejane Nina Martins, Flávia do Carmo Leão Pontes, Emerson Garcia de Almeida, Erich Vinícius de Paula, Olindo Assis Martins-Filho, Adriana Malheiro

“…High expression of TLR2 in monocytes and VLA-4 in TCD8+ lymphocytes and high levels of MIP-1β and RANTES appear to be relevant in high death risk conditions. …”
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Exploring the comorbidity mechanisms between atherosclerosis and hashimoto’s thyroiditis based on microarray and single-cell sequencing analysis by Yirong Ma, Shuguang Wu, Junyu Lai, Qiang Wan, Jingxuan Hu, Yanhong Liu, Ziyi Zhou, Jianguang Wu

“…Single-cell analysis demonstrated predominant expression of the core genes in macrophages, monocytes, T cells and Common Myeloid Progenitor (CMP). …”
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Some haematological values in the healthy kangol dogs and german wolfdogs by Zafer Durgun, Mursayettin Eksen, Ercan Keskin

“…<p> In this study, band neutrophiles, polymorphnuclear neutrophiles, lymphocytes, monocytes and eosinophiles were determined as 2.00, 66.56, 22.35, 4.36 and 4.73 % in male Kangal dogs, whereas the same values found to be 0.86, 65.43, 26.43, 3.43 and 3.86 % in female Kangal dogs, respectively. …”
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Novel role of microphthalmia-associated transcription factor in modulating the differentiation and immunosuppressive functions of myeloid-derived suppressor cells by Yoon Kyung Jeon, Aram Lee, Haesun Park, Soyoung Lim, Jihyun Lim, Jaemoon Koh, Young Yang, Myeong-Sok Lee, Jong-Seok Lim

“…Furthermore, the number of MITF+ monocytic (MO)-MDSCs was examined in human tumor tissues or tumor-free lymph nodes by immunohistochemistry (IHC).Results The expression of MITF was strongly increased in MO-MDSCs from tumors of breast cancer-bearing mice compared with polymorphonuclear MDSCs. …”
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Deciphering the Role of CD36 in Gestational Diabetes Mellitus: Linking Fatty Acid Metabolism and Inflammation in Disease Pathogenesis by Huang L, Zhang T, Zhu Y, Lai X, Tao H, Xing Y, Li Z

“…As a heavily glycosylated transmembrane protein, CD36 is widely expressed in diverse cell types, including placental trophoblasts, monocytes/macrophages, adipocytes, and pancreatic cells et al. …”
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Circulatory Immune Cells in Cushing Syndrome: Bystanders or Active Contributors to Atherometabolic Injury? A Study of Adhesion and Activation of Cell Surface Markers by Gloria Aranda, Cristina Lopez, Rebeca Fernandez-Ruiz, Yaiza Esteban, Guillermo Garcia-Eguren, Mireia Mora, Irene Halperin, Gregori Casals, Joaquim Enseñat, Felicia A. Hanzu

“…Material and Methods. 16 ACTH-dependent CS (ACTH-D), 10 ACTH-independent (ACTH-ID) CS, and 16 healthy controls (C) were included. Leukocytes (Leuc), monocytes (MN), lymphocytes (Lym), and neutrophils (N) were analyzed by flow cytometry for atherosclerosis previously associated with CDs. …”
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De Novo Acute Myeloid Leukemia with Combined CBFB-MYH11 and BCR-ABL1 Gene Rearrangements: A Case Report and Review of Literature by Venkata Rakesh Sethapati, Ra’ed Jabr, Leyla Shune, Wissam El Atrouni, Patrick R. Gonzales, Wei Cui, Shivani Golem

“…Bone marrow evaluation and flow cytometry analysis were compatible with AML with monocytic features. Cytogenetic studies revealed two-related clones, a minor clone with inv(16) and a major clone with concurrent inv(16) and t(9;22) rearrangements. …”
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Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy by Adnette Fagninou, Magloire Pandoua Nekoua, Darius Sossou, Kabirou Moutairou, Nadine Fievet, Akadiri Yessoufou

“…The frequencies of total CD3+ and CD4+ T cells and nonclassical monocytes significantly increased in insulin-treated T2D pregnant women compared to pregnant controls. …”
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Transition metal-based MOFs for Fenton-like photocatalytic degradation of organic pollutants: Performance, stability, and biocompatibility by S.M. Tikhanova, Yu.A. Tishchenko, E.Yu. Stovpiaga, M. Timofeeva, D.V. Lipin, S.A. Povarov, V.A. Milichko, A.S. Timin, S.A. Shipilovskikh, V.I. Popkov

“…In vitro studies conducted on human monocytes (THP-1), murine embryonic fibroblasts (MEF-NF), breast cancer cells (4T1), melanoma cells (B16-F10), and colorectal cancer cells (CT26) demonstrated high biocompatibility of the developed MOFs. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Secretomes effect was tested in in vitro models of chondrocytes, lymphocytes and monocytes to mimic the OA microenvironment. Results: Within a conserved molecular signature, a divergent fingerprint emerged for ASCs' secretomes collected after expansion in standard FBS/hPL or next-generation S/X formulations. …”
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Identification and Analysis of Key Immune- and Inflammation-Related Genes in Idiopathic Pulmonary Fibrosis by Tan Y, Qian B, Ma Q, Xiang K, Wang S

“…Six distinct immune cells, including naive CD4 T cells, T cells CD4 memory resting, T cells regulatory (Tregs), Monocytes, Macrophages M2, Neutrophils were identified. …”
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Assessing the Causal Relationship Between Various Immune Cells and Attention Deficit Hyperactivity Disorder: Mendelian Randomization Study by Qian Ge, Zhongyan Li, Weijing Meng, Chen Cai, Mengdi Qiu, Yafei Liu, Haibo Zhu

“…Immune cell phenotypes that may act as a safeguard against ADHD included CD3 on resting Treg (OR = 0.925, 95% CI = 0.888–0.963, p = 1.5E−4) and SSC‐A on monocytes (OR = 0.951, 95% CI = 0.924–0.980, p = 8.5E−4), among others. …”
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CCR7 and CD48 as Predicted Targets in Acute Rejection Related to M1 Macrophage after Pediatric Kidney Transplantation by Jie Zhang, Jun Pei, Chengjun Yu, Jin Luo, Yifan Hong, Yi Hua, Guanghui Wei

“…In addition to immunoinfiltration, CD4+T, CD8+T, NK cells, NKT cells, and monocytes increased significantly in the AR group, which was highly consistent with the results of Hub gene screening. …”
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CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli by Thushari Gunawardana, Khawaja Ashfaque Ahmed, Kalhari Goonewardene, Shelly Popowich, Shanika Kurukulasuriya, Ruwani Karunarathana, Lisanework E. Ayalew, Ashish Gupta, Betty Lockerbie, Marianna Foldvari, Suresh K. Tikoo, Philip Willson, Susantha Gomis

“…On the day of hatch (72 hr after CpG-ODN treatment), we collected the spleen and lungs (n=3‐4 per group) and examined the recruitment of macrophages/monocytes, their expression of MHCII and CD40, and the number of CD4+ and CD8+ T-cell subsets in the immunological niches in the spleen and lungs using flow cytometry. …”
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Nutritional strategies for laying hens to address environmental challenges by reducing the nitrogen excretion by Vlaicu Petru Alexandru, Untea Arabela Elena, Panaite Tatiana Dumitra, Cornescu Gabriela Maria, Saracila Mihaela, Varzaru Iulia, Oancea Alexandra Gabriela

“…Results from blood samples showed a significant increase (p<0.05) in RPL group on monocytes and uric acid compared with CON and RPC groups with tendencies for leucocytes, lymphocytes, heterophiles. …”
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miR-10a in Peripheral Blood Mononuclear Cells Is a Biomarker for Sepsis and Has Anti-Inflammatory Function by Guoping Zheng, Guanguan Qiu, Menghua Ge, Jianbiao Meng, Geng Zhang, Jiangmei Wang, Ruoqiong Huang, Qiang Shu, Jianguo Xu

“…Data were validated in a mouse model of sepsis induced via cecal ligation and puncture (CLP) and THP-1 monocytes. Results. miR-10a levels in PBMC at admission were significantly lower in sepsis patients compared with patients with infection and healthy controls. miR-10a levels were negatively correlated with disease severity scores as well as levels for c-reactive protein and procalcitonin. …”
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Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy by Coraline Radermecker, Pascale Hubert, Patrick Roncarati, Stephanie Demoulin, Charlotte Pilard, Marie Ancion, Celia Reynders, Thomas Lerho, Diane Bruyere, Alizee Lebeau, Margot Meunier, Marie-Julie Nokin, Elodie Hendrick, Olivier Peulen, Philippe Delvenne, Michael Herfs

“…Indeed, without affecting the global number of (CD45+) immune cells, drastic reductions of monocytic/granulocytic myeloid-derived suppressor cells (MDSC) and regulatory T lymphocytes, a higher M1/M2 ratio of macrophages as well as an increased activation of both DC and pDC were continually observed following HMGB1 inhibition. …”
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SSL5-AnxA5 Fusion Protein Constructed Based on Human Atherosclerotic Plaque scRNA-Seq Data Preventing the Binding of Apoptotic Endothelial Cells, Platelets, and Inflammatory Cells by Yifei Zhao, Xingyu He, Teng Hu, Tianli Xia, Fangyang Huang, Changming Li, Yiming Li, Fei Chen, Mao Chen, Jun Ma, Yong Peng

“…The <i>SELPLG</i> signaling pathway network demonstrated a higher number of interactions in ACS, while the <i>ANNEXIN</i> signaling pathway network revealed stronger signaling from macrophages toward monocytes in ACS compared to SAP. Competition binding experiments with P-selectin showed that SSL5-AnxA5 induced a decrease in the affinity of PSGL1. …”
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