The effect of tumour necrosis factor-α (TNF-α) muteins on human neutrophils in vitro by H. Tchorzewski, K. Zeman, J. Kantorski, E. Paleolog, M. Kahan, M. Feldmann, M. Kwinkowski, P. Guga, B. Szymanska, P. Parniewski, A. Wilk, J. Jarosz

“…Binding of muteins to PMN was assessed using monoclonal antibodies recognizing either the 55 kDa (p55) or the 75 kDa (p75) TNF receptor subtypes. …”
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Predicting deamidation and isomerization sites in therapeutic antibodies using structure-based in silico approaches by David Hoffmann, Joschka Bauer, Markus Kossner, Andrew Henry, Anne R. Karow-Zwick, Giuseppe Licari

“…As such, the early engineering and selection of chemically stable monoclonal antibodies (mAbs) can substantially mitigate the risk of subsequent failure. …”
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Arterial Hypertension and Interleukins: Potential Therapeutic Target or Future Diagnostic Marker? by Daniela Maria Tanase, Evelina Maria Gosav, Smaranda Radu, Anca Ouatu, Ciprian Rezus, Manuela Ciocoiu, Claudia Florida Costea, Mariana Floria

“…Further research is needed to evaluate whether drugs targeting hypertensive-linked proinflammatory cytokines, such as monoclonal antibodies, could become a new therapeutic option in treating arterial hypertension.…”
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Comparison of HER2-Targeted Antibodies for Fluorescence-Guided Surgery in Breast Cancer by Solmaz AghaAmiri, Jo Simien, Alastair M. Thompson, Julie Voss, Sukhen C. Ghosh, Servando Hernandez Vargas, Sarah Kim, Ali Azhdarinia, Hop S. Tran Cao

“…Here, we examine two approved anti-HER2 monoclonal antibodies (mAbs), trastuzumab and pertuzumab, as potential candidates for the development of immunoconjugates for fluorescence-guided surgery (FGS). …”
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Increased Death of Peripheral Blood Mononuclear Cells after TLR4 Inhibition in Sepsis Is Not via TNF/TNF Receptor-Mediated Apoptotic Pathway by Chien-Ming Chu, Li-Chung Chiu, Chung-Chieh Yu, Li-Pang Chuang, Kuo-Chin Kao, Li-Fu Li, Huang-Pin Wu

“…Stimulated peripheral blood mononuclear cells (PBMCs) with or without lipopolysaccharides (LPS) and high-mobility group box 1 (HMGB1) were cultured with or without TLR4 inhibition using monoclonal antibodies from 20 patients with sepsis. Caspase-3, caspase-8, and caspase-9 activities were measured. …”
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The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin by Jin Zeng, Fuyang Song, Yi Yang, Chenjie Ma, Guangcun Deng, Yong Li, Yujiong Wang, Xiaoming Liu

“…A recombinant His-tagged C. perfringens beta2 (rCPB2) toxin and monoclonal antibodies (McAbs) against CPB2 were generated and characterized by assays of cytotoxicity, immunoblotting, ELISA, neutralization, and immunofluorescence. …”
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Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates by Olubukola O. Funsho-Sanni, Elijah E. Ella, Lawal D. Rogo, Olufunsho S. Sanni, Helen I. Inabo, Sodangi A. Luka, Ismaila Shittu

“…Mutation in this A2 antigenic epitope has been reported to induce escape mutation to monoclonal antibodies generated using the NDV LaSota strain. …”
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Candidate antirheumatic genotherapeutic plasmid constructions have low immunogenicity by T. S. Nepomnyashchikh, T. V. Tregubchak, S. N. Yakubitskiy, O. S. Taranov, R. A. Maksyutov, S. N. Shchelkunov

“…TNF inhibitors such as recombinant receptors or monoclonal antibodies are the most widely used biotherapeutics in clinical practice. …”
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T Cell Repertoire and Inflammatory Bowel Disease by Kenneth Croitoru, David KH Wong, Maria E Baca-Estrada

“…In addition, studies are exploring the T cell receptor repertoire in IBD patients for changes that may provide clues regarding etiopathogenesis. Using monoclonal antibodies to T cell receptor variable-gene products or polymerase chain reaction analysis of variable-gene mRNA expression, the mucosal T cell repertoire has been examined in humans. …”
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Multivariate quantitative analysis of glycan impact on IgG1 effector functions by Tamara Cvijić, Matej Horvat, Jakob Plahutnik, Ana Golob, Jaka Marušič

“…To the best of our knowledge, this is the first report of a systematic approach to quantitatively understand the multivariate impact of glycosylation on the effector functionality of therapeutic monoclonal antibodies, providing valuable tools for advancing therapeutic protein development.…”
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Comparison of the Efficacy and Safety of PD‐1/PD‐L1 Inhibitors in the Treatment of Small Cell Lung Cancer by Qichen Zhang, Xiaojuan Han, Jiayi Liu, Hui Qiao

“…ABSTRACT Objective This study aims to evaluate the efficacy and safety of PD‐1/PD‐L1 inhibitors in treating small‐cell lung cancer (SCLC) and determine the role of PD‐1 monoclonal antibodies in improving patient outcomes. Methods A retrospective analysis was performed on 37 SCLC patients who received PD‐1 or PD‐L1 inhibitors along with chemotherapy at the First Hospital of Lanzhou University between June 2018 and June 2023. …”
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Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-β1-Induced Periodontal Ligament-Fibroblastic Differentiation by Hyun-Jin Kim, Seong-Min Kim, Min-Jeong Choi, Young-Joo Jang

“…Our study was to investigate the mechanism of the fibroblastic process in the periodontal ligament differentiation of hPDLSCs through the discovery of novel markers. One of the monoclonal antibodies previously established through decoy immunization was the anti-LG11 antibody, which recognized Golgi subfamily A member 5 (GOLGA5) as a PDL-fibroblastic progenitor-specific antigen. …”
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Cavernous Hemangioma-Like Kaposi Sarcoma: Histomorphologic Features and Differential Diagnosis by Nilüfer Onak Kandemir, Figen Barut, Banu Doğan Gün, Nilgün Solak Tekin, Sevinç Hallaç Keser, Şükrü Oğuz Özdamar

“…A positive reaction with HHV-8, CD31, CD34, and D2-40 monoclonal antibodies was identified at both cavernous hemangioma-like areas and in immature vascular structures. …”
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Secondary Circulating Prostate Cells Predict Biochemical Failure in Prostate Cancer Patients after Radical Prostatectomy and without Evidence of Disease by Nigel P. Murray, Eduardo Reyes, Nelson Orellana, Cynthia Fuentealba, Leonardo Bádinez, Ruben Olivares, José Porcell, Ricardo Dueñas

“…Serial blood samples were taken during followup after RP, mononuclear cells were obtained by differential gel centrifugation, and CPCs identified using standard immunocytochemistry using anti-PSA monoclonal antibodies. Age, pathological stage (organ confined, nonorgan confined), pathological grade, margin status (positive, negative), extracapsular extension, perineural, vascular, and lymphatic infiltration (positive, negative) were compared with the presence/absence of CPCs and with and without biochemical failure. …”
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Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides by Serkan Yazıcı, Emel Bülbül Başkan, Ferah Budak, Barbaros Oral, Şaduman Balaban Adim, Zübeyde Ceylan Kalin, Güven Özkaya, Kenan Aydoğan, Hayriye Saricaoğlu, Şükran Tunali

“…Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3+, CD4+, CD8+, TCRαβ+, TCRγδ+, CD7+, CD4+CD7+, CD4+CD7−, and CD71+), B cells (HLA-DR+, CD19+, and HLA-DR+CD19+), NKT cells (CD3+CD16+CD56+), and NK cells (CD3−CD16+CD56+). …”
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Inferior Outcomes of Fludarabine–Cyclophosphamide–Rituximab Chemotherapy in Korean Chronic Lymphocytic Leukemia Patients with Concurrent Thrombocytopenia and Anemia by Tong-Yoon Kim, Gi-June Min, Young-Woo Jeon, Seung-Ah Yahng, Seok-Goo Cho, Jong-Mi Lee, Myungshin Kim, Ki-Seong Eom

“…<b>Background/Objectives</b>: Anti-CD20 monoclonal antibodies combined with alkylator-based chemotherapy enhance survival in chronic lymphocytic leukemia (CLL). …”
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Therapeutic efficacy of a potent anti-Venezuelan equine encephalitis virus antibody is contingent on Fc effector function by Jennifer L. Schwedler, Maxwell A. Stefan, Christine E. Thatcher, Peter R. McIlroy, Anupama Sinha, Ashlee M. Phillips, Christopher A. Sumner, Colleen M. Courtney, Christina Y. Kim, Dina R. Weilhammer, Brooke Harmon

“…The development of specific, safe, and potent monoclonal antibodies (Abs) has led to novel therapeutic options for infectious disease. …”
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Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples by Luis Escribano, Alberto Orfao, Jesús Villarrubia, Beatriz Díaz-Agustín, Carlos Cerveró, Agustín Rios, José L. Velasco, Juana Ciudad, José L. Navarro, Jesús F. San Miguel

“…The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. …”
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Caerin 1.1/1.9-mediated antitumor immunity depends on IFNAR-Stat1 signalling of tumour infiltrating macrophage by autocrine IFNα and is enhanced by CD47 blockade by Junjie Li, Yuandong Luo, Quanlan Fu, Shuxian Tang, Pingping Zhang, Ian H. Frazer, Xiaosong Liu, Tianfang Wang, Guoying Ni

“…In this study, we explored the impact of F1/F3 on the tumor microenvironment in a transplanted B16 melanoma model, and further investigated the mechanism of action of F1/F3 using monoclonal antibodies to deplete relevant cells, gene knockout mice and flow cytometry. …”
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Alternative buffer systems in biopharmaceutical formulations and their effect on protein stability by Lebar Blaž, Zidar Mitja, Mravljak Janez, Šink Roman, Žula Aleš, Pajk Stane

“…However, conventional buffers like histidine and phosphate buffers may not always be the optimal choice for all monoclonal antibodies (mAbs). In this study, we investigated the effects of several alternative buffer systems on seven different mAbs, exploring various combinations of ionic strengths, concentrations of the main buffer component, mAb concentrations, and stress conditions. …”
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