Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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The use of bacterial culture for Leptospirosis di- agnosis in female water buffaloes by Rosaura Pérez-Gil, Oriana Gavidia, Juan Torres

“…Finally, it is likely that this bacterium growing in the bacteriological cultures belongs to a serovar different from those in the panel of strains used in MAT, so the cultures were preserved for subsequent studies that contemplate molecular identification. …”
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MT1G promotes iron autophagy and inhibits the function of gastric cancer cell lines by intervening in GPX4/SQSTM1 by Kaiqiang Meng, Jian Song, Fan Qi, Jiamin Li, Zhichao Fang, Liang Song

“…Our research findings elucidate that overexpression of MT1G promotes iron autophagy centered around ARNTL in GC cells via the GPX4/SQSTM1 axis, thereby inhibiting GC cell function and providing a new molecular mechanism and therapeutic target for the development of GC.…”
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Postprogression therapy and confounding for the estimated treatment effect on overall survival in phase III oncology trials by Pavlos Msaouel, Ramez Kouzy, Joseph Abi Jaoude, Ethan B Ludmir, Alexander D Sherry, Timothy A Lin, Esther J Beck, Avital M Miller, Adina H Passy, Gabrielle S Kupferman, Eugene J Koay, Clifton David Fuller, Charles R Thomas, Zachary R McCaw

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Microwave hyperthermia enhances radiosensitization by decreasing DNA repair efficiency and inducing oxidative stress in PC3 prostatic adenocarcinoma cells by Yajun Wu, Pengyuan Liu, Wendy Chen, Shiting Bai, Sisi Chen, Jianglin Chen, Xiaogang Xu, Jindan Xia, Yufei Wu, Jianjun Lai, Chuan Sun, Zhenghong Lao, Xiaoqing Wan, Zhibing Wu

“…In this study, we aimed to explore the use of microwave hyperthermia (MW-HT) to sensitize PCa to RT and investigate the underlying molecular mechanisms.Methods We developed a dedicated MW-HT heating setup, created an in vitro and in vivo MW-HT + RT treatment model for CRPC. …”
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From cellular senescence towards age-related macular degeneration: the role of telomeres by L.K. Moshetova, O.I. Abramova, K.I. Turkina, O.P. Dmitrenko, N.S. Karpova

“…The understanding of molecular mechanisms involved in aging may provide innovative therapeutic and preventive strategies for AMD. …”
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Neoadjuvant Immunotherapy and De-escalation of Surgery in Locally Advanced Breast Implant-associated Anaplastic Large Cell Lymphoma by Marzia Salgarello, Jaroslaw Krupa, Rebecca Allchin, Simon Pilgrim, Fiona Miall, Arianna Di Napoli, Maurizio Martelli, Giulio Tarantino

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Evolutive radiological findings in a case of coccidioidal infection in an immunocompetent male: a case report by Paolo La Montanara, Annamaria Albergo, Loredana Capozzi, Roberto Castellana, Gerardo Cazzato, Carmela De Carlo, Sofia Denicolò, Antonella Netti, Mariacristina Poliseno, Enrica Maddalena Ranieri, Caterina Romano, Annalisa Rossiello, Stefania Stolfa, Alessandra Tripoli, Giuseppe Vella

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Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress by Xiao Wu, Yaoran Fan, Jianbin Feng, Keyi Ma, Jiale Li

“…These results could be helpful for further understanding the molecular mechanisms underlying the thermal response of M. nipponense, and increasing knowledge of the regulation mechanisms of freshwater crustaceans in the context of global warming.…”
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Deciphering the Transcriptional Metabolic Profile of Adipose-Derived Stem Cells During Osteogenic Differentiation and Epigenetic Drug Treatment by Giulia Gerini, Alice Traversa, Fabrizio Cece, Matteo Cassandri, Paola Pontecorvi, Simona Camero, Giulia Nannini, Enrico Romano, Francesco Marampon, Mary Anna Venneri, Simona Ceccarelli, Antonio Angeloni, Amedeo Amedei, Cinzia Marchese, Francesca Megiorni

“…However, a deeper understanding of the molecular mechanisms underlying ASC biology is crucial to identifying key regulatory pathways that influence differentiation and enhance regenerative potential. …”
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The forgotten link: how the oral microbiome shapes childhood growth and development by Raymond Rubianto Tjandrawinata, Nurlinah Amalia, Nurlinah Amalia, Nurlinah Amalia, Yosi Yohanes Putra Tandi, Ariq Fadhil Athallah, Caesaroy Afif Wibowo, Muhammad Reva Aditya, Muhammad Reva Aditya, Athaya Rahmanardi Muhammad, Athaya Rahmanardi Muhammad, Maghfira Rahma Azizah, Maghfira Rahma Azizah, Farizky Martriano Humardani, Ammar Nojaid, Jeremy Alvaro Christabel, Alfi Agnuristyaningrum, Fahrul Nurkolis, Fahrul Nurkolis

“…Future research should focus on elucidating the molecular pathways linking the oral microbiome to stunting and developing personalized interventions that optimize microbiome health in early life.…”
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E3 ubiquitin ligase SYVN1 as a promising therapeutic target for diverse human diseases by Li Zhu, Yong-Ping Liu, Yuan-Wang, Bo-Xuan Sun, Yu-Ting Huang, Ji-Kai Zhao, Jian-Feng Liu, Li-Ming Yu, Hui-Shan Wang

“…SYVN1, an E3 ubiquitin ligase, is characterized by its significant functions in regulating various biological processes, including molecular mechanisms related to gene expression, signaling pathways, and cell death, among others. …”
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Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…Therefore, it was proposed that DBF4 could serve as a novel potential prognostic biomarker and molecular target for ccRCC. Clinical trial number Not applicable.…”
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A Cu(I)-Based MOF with Nonlinear Optical Properties and a Favorable Optical Limit Threshold by Jing Cui, Zhaohui Yang, Yu Zhang, Zhaoxuan Fan, Jianquan Wang, Xiaoyun Qin, Lijun Gao, Haoran Yang, Shuangliang Liu, Liming Zhou, Shaoming Fang, Zhen Zhang

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Los olvidados: Non-BRCA variants associated with Hereditary breast cancer in Mexican population by Dione Aguilar, María Lourdes Garza-Rodríguez, Carolina Elizabeth Muñiz-Garza, Fernando Alcorta Nuñez, Cynthia Mayte Villarreal-Garza, Oscar Vidal-Gutiérrez, Diana Cristina Pérez-Ibave, Carlos Horacio Burciaga-Flores

“…In analyzing GPVs in molecular pathways, both have similar frequencies with no statistical difference for BC. …”
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Ursolic acid attenuates obesity-related metabolic dysfunction via modulation of peroxisome proliferator activated receptor-gamma in male Wistar rats fed with high-carbohydrate high... by Oluwatosin O. Omodara, Mohammed U. Kawu, Ibrahim G. Bako, Daniel H. Mhya, Theophilus T. Dawus

“…The present study aimed to elucidate the molecular mechanisms underlying the preventive effects of dietary UA supplementation on obesity-related metabolic disorders and inflammation in male Wistar rats fed with HCHFD. …”
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Early Transcriptional Changes Induced by Wnt/β-Catenin Signaling in Hippocampal Neurons by Eduardo Pérez-Palma, Víctor Andrade, Mario O. Caracci, Bernabé I. Bustos, Camilo Villaman, Matías A. Medina, Miguel E. Ávila, Giorgia D. Ugarte, Giancarlo V. De Ferrari

“…Likewise, following activation of the signaling cascade, the expression of a significant number of genes with transcription factor activity (GO:0043565, p-adjusted = 4.1 × 10−6) was induced. We also studied molecular networks enriched upon Wnt3a activation and detected three highly significant expression modules involved in glycerolipid metabolic process (GO:0046486, p-adjusted = 4.5 × 10−19), learning or memory (GO:0007611, p-adjusted = 4.0 × 10−5), and neurotransmitter secretion (GO:0007269, p-adjusted = 5.3 × 10−12). …”
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Evaluation of the merit of ethanolic extract of Annona reticulata as an anti-cancer agent in human colon cancer cell lines (HCT-116) [version 2; peer review: 2 approved, 1 not appr... by Sri Pragnya Cheruku, Krishnananda Prabhu, Akshatha G Nayak, Vijetha Shenoy Belle, Vanishree Rao, Pooja Prakash Rao, Nitesh Kumar

“…This study was aimed to explore the anti-proliferative and anti-migratory activity of various fractions of Annona reticulata ethanolic leaf extract on human colon cancer cell lines (HCT-116) and to explore the potential molecular targets from the most potent plant extract fraction. …”
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Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice by Peng Liu, Chen Gao, Wen Li, Wanchao Chen, Zhong Zhang, Di Wu, Tingzhao Li, Shuai Sun, Yan Yang

“…Herein, S. rugosoannulata protein (SRA) with mainly molecular weight of 15, 40 and 50 kDa, complete amino acid composition and typical α-helix structure, was obtained by wet pulverization combined with temperature cycling strategy. …”
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Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease by Angel D'Oliviera, Xuhang Dai, Saba Mottaghinia, Sophie Olson, Evan P Geissler, Lucie Etienne, Yingkai Zhang, Jeffrey S Mugridge

“…Finally, enzymology and molecular dynamics simulations indicate that kinetic discrimination occurs during a later step of Mpro-mediated proteolysis following substrate binding. …”
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