Corticostriatal glutamate‐mediated dynamic therapeutic efficacy of electroacupuncture in a parkinsonian rat model by Xinxin Jiang, Min Sun, Yitong Yan, Yanhua Wang, Xinyu Fan, Jing Wei, Ke Wang, Peirong Liang, Zirui Wang, Jihan Wang, Xiaomin Wang, Jun Jia

“…Additionally, we evaluated corticostriatal spine plasticity using immunoelectron microscopy and measured the levels of dopaminergic and glutamatergic neurotransmitters through microdialysis, in vivo electrochemistry and high‐performance liquid chromatography. …”
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Turning the Surface Electronic Effect Over Core‐Shell CoS2─FexCo1‐xS2 Nanooctahedra Toward Electrochemical Water Splitting in the Alkaline Medium by Lian‐Ming Lyu, Yu‐Chung Chang, Han‐Jung Li, Pei‐En Wang, Ruei‐Hung Juang, Ming‐Yen Lu, Cheng‐Shiuan Li, Chun‐Hong Kuo

“…By various X‐ray spectroscopies as well as electron microscopy, the results showed that the shells of CoS2─FexCo1‐xS2 nanooctahedra formed the {111} surfaces of Fe0.9Co1.0S2 and Fe0.25Co0.75S2 with and without the promotion by OH− anions during the syntheses. …”
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Nestin Forms a Flexible Cytoskeleton by Means of a Huge Tail Domain That Is Reversibly Stretched and Contracted by Weak Forces by Ayana Yamagishi, Rina Tokuoka, Kazuki Imai, Mei Mizusawa, Moe Susaki, Koki Uchida, Saku T. Kijima, Akira Nagasaki, Daijiro Takeshita, Chiaki Yoshikawa, Taro Q. P. Uyeda, Chikashi Nakamura

“…In vitro tensile tests using atomic force microscopy were performed to assess the mechanical properties of NTDs. …”
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Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections by Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, Dan Xing, Ye Yuan, Donghui Ding, Ge Shen, Min Yang, Ronjie Wu, Ling Wang, Luiza Martins Nascentes Melo, Sien Lin, Xiaoguang Cheng, Gang Li, Alpaslan Tasdogan, Jessalyn M. Ubellacker, Hu Zhao, Shentong Fang, Bo Shen

“…The bone hemisections are compatible with diverse antibody labeling strategies. Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software, assessing a spectrum of parameters. …”
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Increased interferon I signaling, DNA damage response and evidence of T-cell exhaustion in a patient with combined interferonopathy (Aicardi-Goutières Syndrome, AGS) and cohesinopa... by Sorina Boiu, Nikolaos Paschalidis, George Sentis, Theodora Manolakou, Andrianos Nezos, Manolis Gialitakis, Maria Grigoriou, Erato Atsali, Melpomeni Giorgi, Argirios Ntinopoulos, Clio Mavragani, Periklis Makrythanasis, Dimitrios T. Boumpas, Aggelos Banos

“…DNA damage response was assayed by confocal microscopy in the peripheral blood of this patient. …”
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Impacts of high CDW levels on the chemical, microstructural, and mechanical behavior of cement-based mortars by M.L. Peixoto, S.D. Jesus, H.S. Cavalcante, B.S. Teti, R.C. Manta, N.B. Lima, H.C.B. Nascimento, S. Fucale, N.B.D. Lima

“…The mortars were made to evaluate the mechanical behavior and characterization tests of recycled and natural aggregates, consistency index, mass density, resistance to simple compression, x-ray diffraction, and scanning electron microscopy. The results revealed that the grains' maximum characteristic dimension corresponds to 2.36 mm, equivalent to the fine sand used. …”
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Bone marrow mesenchymal stem cell-derived extracellular vesicles alleviate diabetes-exacerbated atherosclerosis via AMPK/mTOR pathway-mediated autophagy-related macrophage polariza... by Libo Liu, Ziyu An, Huan Zhang, Xueqi Wan, Xin Zhao, Xueyao Yang, Jinfan Tian, Xiantao Song

“…Methods EVs were isolated from the supernatant of cultured BMSCs and characterized with transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting. …”
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Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal... by Nanquan Rao, Xiaotong Wang, Jing Xie, Jingzhi Li, Yue Zhai, Xiaoxia Li, Tengjiaozi Fang, Yuanyuan Wang, Yuming Zhao, Lihong Ge

“…At 8 weeks after administration, real-time PCR, immunohistochemistry (IHC), and electron microscopy were employed to examine pathological changes in glomerular and tubulointerstitial tissue. …”
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Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei by Maznah Alhesain, Ayman Alzu’bi, Ayman Alzu’bi, Ayman Alzu’bi, Niveditha Sankar, Niveditha Sankar, Charles Smith, Charles Smith, Janet Kerwin, Ross Laws, Susan Lindsay, Gavin J. Clowry

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Protein kinases MpkA and SepH transduce crosstalk between CWI and SIN pathways to activate protective hyphal septation under echinocandin cell wall stress by Alexander G. Doan, Jessica E. Schafer, Casey M. Douglas, Matthew S. Quintanilla, Meredith E. Morse, Harley Edwards, Walker D. Huso, Kelsey J. Gray, JungHun Lee, Joshua K. Dayie, Steven D. Harris, Mark R. Marten

“…To better understand this crosstalk, we used a microscopy-based assay to measure septum density, aiming to understand the septation response to cell wall stress. …”
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Study on the Regulated Cell Death of Hypertrophic H9c2 Cells Induced by Au:Ag Nanoparticles by Galindo-Padrón AG, Lorenzo-Anota HY, Rueda-Munguía M, García-Carrasco A, Gaitán López M, Vázquez-Garza E, Campos-González E, Lozano O, Cholula-Díaz JL

“…Their physicochemical properties were characterized through UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), ζ-potential measurements, and transmission electron microscopy (TEM). Moreover, the effect of the metal-based nanoparticle exposure on healthy and hypertrophic H9c2 cells was measured by analyzing the cellular vitality, the loss of mitochondrial membrane potential (∆Ψm), and the production of mitochondrial reactive oxygen species (mROS).Results: The Au:Ag NPs did not affect the cell vitality of healthy or metabolically activated macrophages. …”
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Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β-Lactam Antibiotics by Dyana Leal Veras, Ana Catarina de Souza Lopes, Grasielle Vaz da Silva, Gabriel Gazzoni Araújo Gonçalves, Catarina Fernandes de Freitas, Fernanda Cristina Gomes de Lima, Maria Amélia Vieira Maciel, Ana Paula Sampaio Feitosa, Luiz Carlos Alves, Fábio André Brayner

“…In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. …”
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Investigation the effect of calcination heating rate on the structural, morphological, thermal and color properties of nano Cobalt Aluminate (CoAl2O4) by Md. Farid Ahmed, Monmon Podder, Md. Rassel Moni, Md. Lutfor Rahman, Bristy Biswas, Juliya Khanam, Mahmuda Hakim, Moksodur Rahman, Md. Sahadat Hossain, Nahid Sharmin

“…The presence of functional groups was explored by Fourier Transform Infrared (FTIR) Spectroscopy, while Field Emission Scanning Electron Microscopy (FESEM) illustrated agglomerated nanoparticles showcasing diverse shapes. …”
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Hsp70/Bmi1-FoxO1-SOD Signaling Pathway Contributes to the Protective Effect of Sound Conditioning against Acute Acoustic Trauma in a Rat Model by Guoxia Zhu, Yongxiang Wu, Yang Qiu, Keyong Tian, Wenjuan Mi, Xinqin Liu, Yuanyuan Chen, Jinwen Jia, Jiasheng Luo, Lianjun Lu, Jianhua Qiu

“…Moreover, the auditory brainstem responses (ABRs) and electron microscopy revealed that SC could protect against acute acoustic trauma (AAT) based on a significant reduction of hearing impairment and visible reduction in outer hair cell loss as well as ultrastructural changes in OHCs and SGNs. …”
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Comprehensive Structural, Chemical, and Optical Characterization of Cu₂ZnSnS₄ Films on Kapton Using the Automated Successive Ionic Layer Adsorption and Reacti... by Perla J. Vázquez-González, Martha L. Paniagua-Chávez, Lizette A. Zebadua-Chavarria, Rafael Mota-Grajales, C. A. Meza-Avendaño, Enrique Campos-González, A. Escobosa-Echavarría, Yaoqiao Hu, Aldo E. Pérez-Ramos, Manuel-Matuz, Carlos A. Hernández-Gutiérrez

“…Field emission scanning electron microscopy (FE-SEM) showed enhancements in surface morphology, with increased grain size and uniformity post-annealing. …”
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Leveraging AI to automate detection and quantification of extrachromosomal DNA to decode drug responses by Kohen Goble, Aarav Mehta, Damien Guilbaud, Jacob Fessler, Jingting Chen, William Nenad, William Nenad, Christina G. Ford, Oliver Cope, Darby Cheng, William Dennis, Nithya Gurumurthy, Yue Wang, Kriti Shukla, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk

“…Extrachromosomal DNA (ecDNA), in contrast, represents a rapid, reversible, and predictable DNA alteration critical for cancer’s adaptive response.MethodsIn this study, we developed a novel post-processing pipeline for automated detection and quantification of ecDNA in metaphase Fluorescence in situ Hybridization (FISH) images, leveraging the Microscopy Image Analyzer (MIA) tool. This pipeline is tailored to monitor ecDNA dynamics during drug treatment.ResultsOur approach effectively quantified ecDNA changes, providing a robust framework for analyzing the adaptive responses of cancer cells under therapeutic pressure.DiscussionThe pipeline not only serves as a valuable resource for automating ecDNA detection in metaphase FISH images but also highlights the role of ecDNA in facilitating swift and reversible adaptation to epigenetic remodeling agents such as JQ1.…”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD). …”
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Evaluating oxide nanoparticle exsolution on A-site deficient PrBaCo2O6-δ electrodes by Alfonso J Carrillo, María Balaguer, Cecilia Solís, Andrés López-García, Sylvio Haas, María Fabuel, Blanca Delgado-Galicia, Isabelle Rodriguez, Einar Vøllestad, Sebastian Wachowski, Ragnar Strandbakke, Truls Norby, Jose M Serra

“…Combined Raman spectroscopy and electron microscopy analysis revealed that BaCoO _3 , Co _3 O _4 , and amorphous BaCO _3 nanoparticles were formed on the surface of the double perovskites after the reductive treatments. …”
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Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0... by Alena S. Ovchinnikova, Dmitrii A. Odnoshevsky, Alexey S. Kabanov, Sergey A. Bodnev, Oleg V. Pyankov, Oksana P. Os'kina, Maria V. Sivay, Andrey V. Bespalov, Tatyana V. Tregubchak, Larisa N. Shishkina, Oleg S. Taranov, Vladimir V. Zolin, Artemiy A. Sergeev, Alexander P. Agafonov

“…In this study, the pathogen of monkeypox was detected and identified using real-time PCR, NGS and electron microscopy, and the first imported case of this disease in Russia was confirmed. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Biofilm viability was assessed using CAM/TMA-DPH or SYTO9/PIstaining, followed by analysis with confocal laser scanning microscopy (CLSM) and ImageJ-based biofilm surface coverage quantification. …”
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