Pore Structure and Its Fractal Dimension: A Case Study of the Marine Shales of the Niutitang Formation in Northwest Hunan, South China by Wei Jiang, Yang Zhang, Tianran Ma, Song Chen, Yang Hu, Qiang Wei, Dingxiang Zhuang

“…To analyze the pore structure and fractal characteristics of marine shale in the lower Cambrian Niutitang Formation in northwestern Hunan Province, China, the pore characteristics of shale were characterized using total organic carbon (TOC) content, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), low temperature nitrogen adsorption (LT-N₂GA) and methane adsorption experiments. …”
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Itraconazole promotes melanoma cells apoptosis via inhibiting hedgehog signaling pathway-mediated autophagy by Shunqiao Jin, Shunqiao Jin, Xiaojiao Liu, Xiaojiao Liu, Lingqin Cai, Lingqin Cai, Jiayu Yan, Ling Li, Hongjun Dong, Yuxue Gao, Xicong Zhu, Cong Zhang, Xuezhu Xu

“…Western blot analysis was performed to examine autophagy and associated signaling proteins, while autophagy flux and autophagosome formation were visualized using fluorescence microscopy. A melanoma cell xenograft mouse model was established to evaluate the inhibitory mechanisms of itraconazole on tumor cell proliferation.ResultUsing the PMF machine learning algorithm, SQSTM1 was identified as the primary target of itraconazole. …”
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Eco-Friendly Synthesis of Zirconium Dioxide Nanoparticles from <i>Toddalia asiatica</i>: Applications in Dye Degradation, Antioxidant and Antibacterial Activity by Arumugam Kathirvel, Ramalingam Srinivasan, Sathasivam Harini, Natarajan Ranjith, Govindan Suresh Kumar, Kesavan Lalithambigai, Raji Atchudan, Mohamed A. Habila, Ahmed M. Aljuwayid, Hae Keun Yun

“…Analytical techniques, including various spectroscopy methods and electron microscopy, confirmed the formation of aggregated spherical ZrO₂ NPs, ranging from 15 to 30 nm in size, with mixed-phase structure composed of tetragonal and monoclinic structures. …”
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Polydomus karssenii gen. nov. sp. nov. is a dark septate endophyte with a bifunctional lifestyle parasitising eggs of plant parasitic cyst nematodes (Heterodera spp.) by Samad Ashrafi, Jan-Peer Wennrich, Yvonne Becker, Jose G. Maciá-Vicente, Anke Brißke-Rode, Matthias Daub, Torsten Thünen, Abdelfattah A. Dababat, Maria R. Finckh, Marc Stadler, Wolfgang Maier

“…Confocal laser scanning microscopy further demonstrated that the fungus colonised the root cells by predominant intercellular growth of hyphae, and frequent formation of appressorium-like as well as penetration peg-like structures through internal cell walls surrounded by callosic papilla-like structures. …”
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WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota by Qi Sun, Bai-Rong Li, Dong-Hao Li, Xiao-Ying Wang, Qian-Yi Wang, Zhi-Meng Jiang, Shou-Bin Ning, Tao Sun

“…Epithelial barrier integrity was evaluated using immunofluorescence, transmission electron microscopy, and permeability assays. EGCs activity was analyzed via immunofluorescence and quantitative real-time PCR. …”
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Ionizing radiation and photodynamic therapy lead to multimodal tumor cell death, synergistic cytotoxicity and immune cell invasion in human bladder cancer organoids by Annabell Reinhold, Annegret Glasow, Sandra Nürnberger, Annett Weimann, Lucie Telemann, Jens-Uwe Stolzenburg, Jochen Neuhaus, Mandy Berndt-Paetz

“…Cytotoxicity was determined by microscopy, cell-based assays and histology. The cell death mode was analyzed by applying specific inhibitors followed by immunofluorescence or qPCR analyses of cell death markers. …”
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Pore Preservation and Failure Mechanism of Sinian Dengying Formation Carbonate Reservoirs: A Case Study of Two Ultradeep Wells in the Sichuan Basin, Western China by Zeqi Li, Wei Sun, Shugen Liu, Zhiwu Li, Bin Deng, Juan Wu, Yi Ding, Kun Jiao, Xin Jin, Pengda Lu, Tengzhen Tian

“…This study performed thin-section, geochemistry, field emission scanning electron microscopy, fluid inclusion, and basin model analysis of samples from two boreholes over 8,000 m deep in the Sichuan Basin to determine the pore features and preservation mechanism of the Sinian (Ediacaran) Dengying Formation carbonate reservoirs. …”
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Halloysite nanotubes as delivery mechanism for feather protein-based multi-surfactant systems in crude oil dispersion application by Yaw Kwakye Adofo, Emmanuel Nyankson, Benjamin Agyei-Tuffour, Selassie Gbogbo, Christian Amoako, Joseph Arko Morgan, Gloria Pokuaa Manu, Grace Karikari Arkorful

“…Chicken feather protein (CFP), and lecithin (L), Tween 80 (T), and DOSS (D) surfactant systems were loaded onto halloysite nanotubes (HNTs), a natural clay aluminosilicate product to form particulate dispersants at different surfactant concentrations and characterized by Fourier Transform Infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy coupled with EDS. For 24 wt% surfactants concentrations loaded HNTs; 100 wt%CFP-HNTs, 60 wt%CFP- 40 wt%L-HNTs, 20 wt%CFP- 80 wt%T-HNTs, 50 wt%CFP- 25 wt%T-25 wt%L-HNTs and 25 wt %CFP- 25 wt%T-50 wt%D-HNTs showed good interfacial tension lowering abilities by recording 6.39, 2.82, 2.43, 1.68, and 1.55 mN/m at 60s respectively. …”
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Layered metal carbo–selenide Nb2CSe2 with van der Waals interlayer coupling by Junchao Wang, Renfei Cheng, Tao Hu, Xinyue Tang, Yan Liang, Min Tian, Ting Xiong, Qiuhong Lu, Qi Zhu, Zhiqing Yang, Xiaohui Wang, Yanchun Zhou

“…The stacking structure of Nb2CSe2, a newly synthesized layered metal carbo-selenide, was elucidated by scanning transmission electron microscopy. Nb2CSe2 features Se−Nb−C−Nb−Se quintuple atomic layers. …”
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Corneal Reinnervation and Sensitivity Recovery after Pterygium Excision by ZhanLin Zhao, JiaYing Zhang, Hong Liang, SiYi Zhang, ChunYi Shao, XianQun Fan, Yao Fu

“…The central cornea was analyzed using in vivo confocal microscopy (IVCM) before surgery and 1 and 3 months after surgery. …”
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Human parietal epithelial cells as Trojan horses in albumin overload by Giovanna Priante, Monica Ceol, Lisa Gianesello, Claudia Maria Radu, Rachele Mantese, Lucia Federica Stefanelli, Martina Cacciapuoti, Francesca K. Martino, Lorenzo Arcangelo Calò, Franca Anglani, Federico Nalesso, Dorella Del Prete

“…Cell morphology was analyzed by phase-contrast microscopy and F-actin staining. We demonstrate that hPECs internalize albumin via receptor-mediated mechanisms. …”
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The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation by Humberto Osvaldo Schwartz-Filho, Kostas Bougas, Paulo G. Coelho, Ying Xue, Mariko Hayashi, Rafael Silveira Faeda, Rosemary Adriana Chiérici Marcantonio, Daisuke Ono, Fumio Kobayashi, Kamal Mustafa, Ann Wennerberg, Ryo Jimbo

“…After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). …”
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Experimental study on the engineering characteristics of expansive soil improved conjointly using enzyme induced carbonate precipitation and eggshell powder by Mudassir Mehmood, Yuancheng Guo, Yunlong Liu, Lei Wang, Wen Nie, Bantayehu Uba Uge, Sharafat Ali, Chen Xuanyu, Yingao Zhao

“…A thorough examination of the soil’s microstructure development after treatment was additionally executed through scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. …”
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Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells by Misaki Yagi, Mitsuru Mizuno, Ryota Fujisawa, Hisako Katano, Kentaro Endo, Nobutake Ozeki, Yuriko Sakamaki, Hideyuki Koga, Ichiro Sekiya

“…Suspensions of human synovial MSCs were plated on PLA films with different pore sizes (no pores, or with 5 μm or 20 μm pores) and then observed by scanning electron microscopy. The numbers of cells remaining in the film and passing through the film were quantified. …”
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Exploring the Anticancer Potential of MonoHER (7-Mono-O-(β-Hydroxyethyl)-Rutoside): Mitochondrial-Dependent Apoptosis in HepG2 Cells by Chujie Li, Yue Wang, Jian Liang, Guido R. M. M. Haenen, Yonger Chen, Zhengwen Li, Ming Zhang, Ludwig J. Dubois

“…The effects of monoHER on apoptosis proteins were detected by confocal microscopy analysis and Western blot. Results: It was found that MonoHER can reduce HepG2 cells’ and MCF7 cells’ viability, but not H1299 cells’, and induced apoptosis only in HepG2 cells. …”
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Reestablishment of Redox Homeostasis in the Nociceptive Primary Afferent as a Mechanism of Antinociception Promoted by Mesenchymal Stem/Stromal Cells in Oxaliplatin-Induced Chronic... by Anna Lethicia L. Oliveira, Gisele G. L. Santos, Renan F. Espirito-Santo, Gessica Sabrina A. Silva, Afrânio F. Evangelista, Daniela N. Silva, Milena B. P. Soares, Cristiane Flora Villarreal

“…Four weeks later, mitochondrial morphology, gene expression profile, and oxidative stress markers in the sciatic nerve and dorsal root ganglia (DRG) were evaluated by transmission electron microscopy, RT-qPCR, and biochemical assays, respectively. …”
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Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand by Panadda Krairojananan, Kasima Wasuworawong, Surachai Leepitakrat, Taweesak Monkanna, Elizabeth W. Wanja, Silas A. Davidson, Betty K. Poole-Smith, Patrick W. McCardle, Alyssa Mann, Erica J. Lindroth

“…A surveillance program was conducted at Engineering Civil Assistance Program (ENCAP) training sites using real-time PCR, dark-field microscopy, and 16S rRNA gene sequencing to detect <i>Leptospira</i> in rodents and environmental samples. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…Treated and control tumors were isolated at various time points to assess transcriptional changes associated with VN and TLS formation via quantitative PCR (qPCR), with corollary immune cell composition changes in isolated tissues determined using flow cytometry and immunofluorescence microscopy. In vitro assays were performed on CD11c+ BMDCs treated with 2.5 µg/mL ADU S-100 or CD11c+ DCs isolated from tumor digests and associated transcriptional changes analyzed via qPCR or profiled using DNA microarrays. …”
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Exosomal miR-122 derived from M2 macrophages induces osteogenic differentiation of bone marrow mesenchymal stem cells in the treatment of alcoholic osteonecrosis of the femoral hea... by Guoping Le, Riyou Wen, Huaixi Fang, Zhifa Huang, Yong Wang, Hanwen Luo

“…M2 macrophages were identified by flow cytometry, and the isolated exosomes were characterized by transmission electron microscopy (TEM) and Nanoparticle Tracking Analysis (NTA). …”
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Adsorption of Chromium(VI) onto Freshwater Snail Shell-Derived Biosorbent from Aqueous Solutions: Equilibrium, Kinetics, and Thermodynamics by Xuan Hoa Vu, Lan Huong Nguyen, Huu Tap Van, Dinh Vinh Nguyen, Thu Huong Nguyen, Quang Trung Nguyen, L. T. Ha

“…The FSS biosorbent was characterized using nitrogen adsorption/desorption isotherm, X-ray diffraction, scanning electron microscopy with energy dispersive spectroscopy, and Fourier transform infrared spectroscopy. …”
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