The gut–kidney axis is regulated by astragaloside IV to inhibit cyclosporine A-induced nephrotoxicity by Cong Han, Ran-ran Gao, Le Zhou, Wei Li

“…This study aimed to verify the ameliorative effects of AS-IV on CICN and further explore the mechanisms underlying the modulation of the “gut–transcriptome–metabolome coexpression network” by AS-IV within the context of the “gut–kidney axis” to improve CICN.MethodsFive groups of 40 mice were studied: a normal group (N, olive oil), a model group (M, CsA, 30 mg kg-−1 d−1), a low-dose AS-IV group (CsA + AS-IV, 30 mg kg−1 d−1 + 10 mg kg−1 d−1), a high-dose AS-IV group (CsA + AS-IV, 30 mg kg−1 d−1 + 20 mg kg−1 d−1), and a valsartan group (CsA + Val, 30 mg kg−1 d−1 + 10 mg kg−1 d−1). …”
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The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network by Yi Wang, Yi Wang, Moran Xu, Xiaoli Zuo, Sheng Wang, Yong Yu, Zhaobing Gao, Zhaobing Gao, Jingbo Qie, Ye Jiang, Fang Huang, Bingqing Xia, Bingqing Xia

“…Furthermore, we established an intracerebral hemorrhage model by collagenase IV injection in WT and Mlkl-/- mice. Subsequently, we investigated the impact of MLKL knockout on ICH pathological damage through behavioral tests, Western blotting, and RT-qPCR. …”
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Estrogen-related receptor gamma is a regulator of mitochondrial, autophagy, and immediate-early gene programs in spiny projection neurons: Relevance for transcriptional changes in... by Stephanie N. Fox, Cody H. Savage, Narcy R. Amireddy, Laura J. McMeekin, David K. Crossman, Peter J. Detloff, Michelle Gray, Rita M. Cowell

“…Concordantly, Esrrg-deficient mice exhibited lack of amphetamine-induced hyperactivity and further upregulation of immediate-early genes. …”
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The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner by Tomoko Okuyama, Takahiro Tsuno, Ryota Inoue, Setsuko Fukushima, Mayu Kyohara, Anzu Matsumura, Daisuke Miyashita, Kuniyuki Nishiyama, Yusuke Takano, Yu Togashi, Makiko Meguro-Horike, Shin-ichi Horike, Tatsuya Kin, A.M. James Shapiro, Hiromi Yanagisawa, Yasuo Terauchi, Jun Shirakawa

“…Fbln5 expression was increased upon glucose stimulation in β-cells of mouse and human islets. β-Cell-specific Fbln5-knockout (βFbln5KO) mice exhibit significantly reduced β-cell proliferation in vivo but not in vitro. …”
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Apolipoprotein A-V Is a Novel Diagnostic and Prognostic Predictor in Pediatric Patients with Sepsis: A Prospective Pilot Study in PICU by Chunxia Wang, Yun Cui, Huijie Miao, Xi Xiong, Jiaying Dou, Lujing Shao, Xiaomeng Tang, Yucai Zhang

“…ApoA5 was screened from serum proteomics profile in lipopolysaccharide- (LPS-) treated mice for 2 h, 24 h, and controls. Then, we conducted a prospective pilot study, and patients with sepsis admitted to a pediatric intensive care unit (PICU) were enrolled from January 2018 to December 2018. …”
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Bleomycin pollution and lung health: The therapeutic potential of peimine in bleomycin-induced pulmonary fibrosis by inhibiting glycolysis by Kangchen Li, Xuefang Liu, Ruilong Lu, Peng Zhao, Yange Tian, Jiansheng Li

“…We demonstrate that BLM induces pulmonary fibrosis in mice and enhances glycolysis and fibroblast activation. …”
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Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY12/P2RY13 related functions by Martin Škandík, Lara Friess, Guillermo Vázquez-Cabrera, Lily Keane, Kathleen Grabert, Mireia Cruz De los Santos, Mercedes Posada-Pérez, Austeja Baleviciute, Mathilde Cheray, Bertrand Joseph

“…Furthermore, a subsequent comparative analysis of aged BV-2 microglia with established transcriptomic microglial datasets from aged mice and humans identified 13 overlapping genes, laying the foundation for identifying core microglial aging signature. …”
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TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3 by Jing Gu, Yang-fan Lv, Ji-ying Xia, Fu-hai Bai, Ji Gong, Guang-qiang Pan, Bo Liu, Lu Huang, Qiao-nan Guo, Xiang-lin Hao

“…Methods TC2N-engineered mice model was used to assess the effect of TC2N knockout on normal lung and urethane-induced carcinogenesis. …”
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Mesenchymal stem cell-mediated adipogenic transformation: a key driver of oral squamous cell carcinoma progression by Yiting Shao, Yu Du, Zheng Chen, Lei Xiang, Shaoqin Tu, Yi Feng, Yuluan Hou, Xiaoxing Kou, Hong Ai

“…Subcutaneous xenograft experiments were conducted in BALB/c-nu mice to further investigate the effects in vivo. …”
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Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…EDB could improve symptoms and the underlying pathogenesis of CCH mice and decrease the expression of the fibrous profiles of pericytes and ECM proteins, which may be regulated by TGF-β1/ IL-11. …”
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Hepatic Abnormal Secretion of Apolipoprotein C3 Promotes Inflammation in Aortic Dissection by Xinghui Zhuang, Mohammad Zarif, Yue Shen, Zhaofeng Zhang, Jian He, Linfeng Xie, Qingsong Wu, Xinfan Lin, Keyuan Chen, Yue Tian, Yong Lin, Yuling Zhang, Ziwen Cai, Zhihuang Qiu, Liangwan Chen

“…Apo C3 in the aortas of AD mice was found to originate from abnormal hepatic secretion, which enters the bloodstream and subsequently deposits in the aorta. …”
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Syngeneic natural killer cell therapy activates dendritic and T cells in metastatic lungs and effectively treats low-burden metastases by Shih-Wen Huang, Yein-Gei Lai, Hao-Ting Liao, Chin-Ling Chang, Ruo-Yu Ma, Yung-Hsiang Chen, Yae-Huei Liou, Zhen-Qi Wu, Yu-Chen Wu, Ko-Jiunn Liu, Yen-Tsung Huang, Jen-Lung Yang, Ming-Shen Dai, Nan-Shih Liao

“…Using a spontaneous metastasis mouse model of MHC-I+ breast cancer, we found that transfer of IL-15/IL-12-conditioned syngeneic NK cells after primary tumor resection promoted long-term survival of mice with low metastatic burden and induced a tumor-specific protective T cell response that is essential for the therapeutic effect. …”
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Absent in melanoma 2: a potent suppressor of retinal pigment epithelial-mesenchymal transition and experimental proliferative vitreoretinopathy by Yu Chen, Mingyuan Jiang, Liping Li, Shanshan Yang, Zuimeng Liu, Shiwen Lin, Wanxiao Wang, Jinyang Li, Feng Chen, Qiang Hou, Xiaoyin Ma, Ling Hou

“…Consequently, Aim2-deficient mice showed morphological changes and increased FN expression in RPE cells under physiological conditions, whereas AIM2 overexpression in phRPE cells inhibited EMT. …”
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GAMG alleviates liver fibrosis through inducing ferroptosis in inflammatory macrophages via the IRF1/SLC7A11 signaling pathway by Qing Pang, Shuai Zhou, Yong Wang, Hongtao Pan, Zhixin Wang, Xilaing Qin, Chao Zhu, Shilei Chen, Huichun Liu, Xiaosi Hu, Hao Jin

“…In this study, GAMG treatment significantly reduced hepatocyte steatosis, fibroplasia, inflammatory cell infiltration, and collagen fiber deposition in LF mice. In addition, GAMG remarkably decreased the content of collagen protein and improved liver function indicators. …”
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NOX4 modulates breast cancer progression through cancer cell metabolic reprogramming and CD8+ T cell antitumor activity by Yingying Xiong, Yiming Weng, Shan Zhu, Jian Qin, Jia Feng, Xiaopeng Jing, Chao Luo, Wei Gong, Rui Sun, Min Peng

“…In contrast, NOX4 overexpression improved survival in mice and synergized with checkpoint blockade, demonstrating a positive effect on anti-tumor immunity.DiscussionThese findings show that NOX4 constrains breast cancer aggressiveness by limiting MYC-driven metabolic adaptations and supporting CD8+ T cell-mediated immunity. …”
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Utilisation of the Innovative [18F]-Labelled Radiotracer [18F]-BIBD-071 Within HR+ Breast Cancer Xenograft Mouse Models by Di Fan, Xin Wang, Xueyuan Ling, Hongbin Li, Lu Zhang, Wei Zheng, Zehui Wu, Lin Ai

“…MCF-7 cells were cultured and implanted into BALB/c nude mice to establish subcutaneous tumour models. MicroPET/CT imaging was conducted after injection of the tracer at 1 and 2 h, and a blocking study was also conducted using the aromatase inhibitor letrozole. …”
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Role of calcium overload-mediated disruption of mitochondrial dynamics in offspring neurotoxicity due to methylmercury exposure during pregnancy and lactation by Lingxu Kong, Huajie Yang, Jing Yang, Liujiangshan Jiang, Bin Xu, Tianyao Yang, Wei Liu

“…Here, we used ICR-pregnant mice and their resulting offspring and administered the BAPTA-AM calcium antagonist to investigate the molecular mechanisms by which MeHg exposure during gestation and lactation affects neurodevelopment. …”
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Comprehensive safety and toxicity analysis of 2,2’-Bipyridine derivatives in combating MRSA biofilm formation and persistence by Priyanka, Priyanka, Mohini Sharma, Bhavna Vaid, Bhavna Vaid, Ram Bharti, Ram Bharti, Sachin Raut, Sachin Raut, R. S. Jolly, Neeraj Khatri, Neeraj Khatri

“…The murine thigh infection model was also used to investigate the in vivo efficacy.ResultsAll derivatives exhibited favorable physicochemical profiles and ADMET properties and are predicted to be safe based on their drug-like properties. in vitro studies demonstrated that derivatives are non-toxic to 3T3 L1, and in vivo studies confirmed their safety in mice at a dose of 300 mg/kg and their non-hemolytic nature against rabbit red blood cells. …”
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Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models by Yu-heng Wei, Shu-mian Zhou, Shu-mian Zhou, Shu-mian Zhou, Wen Zhao, Qi Chen, Qiu-hua Wang, Qiu-hua Wang, Mei-ling Yu, Ying-yi Wei, Ting-jun Hu, Ting-jun Hu, Ting-jun Hu

“…And assigning both RAW264.7 cells and 108 SPF-grade KM mice to Control, PCV2, Rutin, and various dosages of FNB groups. …”
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Asiaticoside Attenuates Chronic Restraint Stress-Induced Hippocampal CA1 Neuronal Ferroptosis via Activating BDNF/Nrf2/GPX4 Signaling Pathway by Zhou A, Feng HY, Fan CN, Wang J, Yuan ZY, Xu GH, Li CF, Huang WF, Yi LT

“…Our research aims to elucidate the potential antidepressant mechanisms of asiaticoside, a bioactive compound known for its neuroprotective and immunomodulatory properties.Methods: The antidepressant-like properties of asiaticoside in a model of chronic restraint stress (CRS)-induced depression in mice, with a particular focus on its interaction with ferroptosis-related pathways.Results: The behavioral results revealed that asiaticoside significantly ameliorated CRS-induced depressive symptoms, as evidenced by increased sucrose preference and reduced immobility time. …”
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