TYRO3 blockade enhances anti-PD-1 therapy response by modulating expression of CCN1 in tumor microenvironment by Miso Park, Da-Sol Kuen, Keon Wook Kang, Yeonseok Chung, Jaewoo Park, Munkyung Choi, Yeonji Kim, Eun Chae Roh, Yong June Choi, Yoon Gyoon Kim, Sung Yun Cho

“…Co-cultures of macrophages and T cells were performed to verify the role of CCN1 in the tumors.Results TYRO3 blockade boosts antitumor immune responses in both the tumor-draining lymph nodes and tumors in MC38-syngeneic mice models. Moreover, the combination of KRCT87 and anti-PD-1 therapy exerts significant synergistic antitumor effects in anti-PD-1-non-responsive 4T1-syngeneic model. …”
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Bacteroides Fragilis Exacerbates T2D Vascular Calcification by Secreting Extracellular Vesicles to Induce M2 Macrophages by Cong Chen, Zhengfeng Liang, Yuqi He, Yan Gao, Shuhui Ouyang, Lili Wang, Jianghua Liu, Jingsong Cao

“…Macrophages clearance significantly antagonized BF EV‐induced T2D VC in mice. Mechanistically, EV‐rich double‐stranded DNA (dsDNA) activates stimulator of interferon response cGAMP interactor 1 (Sting), promotes myocyte enhancer factor 2D (Mef2d) phosphorylation, upregulates tribbles pseudokinase 1 (Trib1) expression, and induces macrophage M2 polarization. …”
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow by Tongling Huang, Zhaocheng Lu, Zihui Wang, Lixin Cheng, Lu Gao, Jun Gao, Ning Zhang, Chang-An Geng, Xiaoli Zhao, Huaiyu Wang, Chi-Wai Wong, Kelvin W. K. Yeung, Haobo Pan, William Weijia Lu, Min Guan

“…Pharmacological inhibition of ESRRA protects obese mice against bone loss and high marrow adiposity. …”
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Spironolactone Protects against Diabetic Cardiomyopathy in Streptozotocin-Induced Diabetic Rats by Wenjuan Liu, Wei Gong, Min He, Yemei Liu, Yeping Yang, Meng Wang, Meng Wu, Shizhe Guo, Yifei Yu, Xuanchun Wang, Fei Sun, Yiming Li, Linuo Zhou, Shengmei Qin, Zhaoyun Zhang

“…Here, we determined the cardioprotective role of SPR in diabetic mice and further explored the potential mechanisms in both in vivo and in vitro models. …”
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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…In addition, in vivo experiments showed that melatonin could reverse the damage caused by TNF-α on bone regeneration by BMMSCs in nude mice. Overall, our results suggest that melatonin can reverse the loss of stemness caused by inflammatory factor TNF-α in BMMSCs. …”
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PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…In vivo, CD274 knockout (KO), CD8a KO, platelet-specific KO (PF4-Cre-Hsp90b1flox/flox) mouse models and a subcutaneous tumour model treated with aspirin and PD-L1 mAb were established in C57BL/6 N mice. Results We found that PD-L1 positive platelets are correlated with a poor prognosis, CD8 + T cell exhaustion and serve as a novel noninvasive biomarker for predicting immunotherapy efficacy in patients with CRC. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…Finally, USP39 downregulation restricted the formation of lung cancer xenografts in nude mice. Conclusions USP39 undergoes LLPS in the nucleolus and promotes tumor progression by regulating GLI1 expression. …”
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MSC-EVs and UCB-EVs promote skin wound healing and spatial transcriptome analysis by Ruonan Li, Haotian Wang, Xiaolong Wang, Yanbin Yang, Kai Zhong, Xuemei Zhang, Heping Li

“…ST was performed on skin tissue samples from mice in each group after wound healing to analyze the heterogeneity of major cell types compared with the control group and investigate potential mechanisms affecting wound healing and scar formation. …”
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N-2-Hydroxypropyl Trimethyl Ammonium Chloride Chitosan-Aluminum Nano-Adjuvant Elicit Strong Immune Responses in Porcine Epidemic Diarrhea Inactivated Vaccine by Jin Z, Liu J, Guo S, Xu S, Gong X, Zhang C, Zhao K

“…PED inactivated vaccine (N-2-HACC-Al/PEDV) was prepared by electrostatic adsorption method, and mice were inoculated by intramural injection and orally to evaluate the immune enhancement effect and application potential of the N-2-HACC-Al/PEDV.Results: The hemolysis rate was 3.89 ± 0.12% and the activity of PK15 cells was 77.40 ± 1.74%, indicating that the N-2-HACC-Al/PEDV had good biosafety. …”
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Comparative Analysis of Immunoactivation by Nanosecond Pulsed Electric Fields and PD-1 Blockade in Murine Hepatocellular Carcinoma by Maiweilidan Yimingjiang, Talaiti Tuergan, Xinhua Chen, Hao Wen, Yingmei Shao, RuiQing Zhang, Kasimu Aihaiti, Jing Xue, Tuerganaili Aji, Wei Zhang

“…Briefly, 24 C57BL-6J tumor-bearing mice were randomly assigned to three groups: nsPEF ablation group, anti-PD-1 administration group, and untreated control group. …”
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Development of Cephradine-Loaded Gelatin/Polyvinyl Alcohol Electrospun Nanofibers for Effective Diabetic Wound Healing: In-Vitro and In-Vivo Assessments by Anam Razzaq, Zaheer Ullah Khan, Aasim Saeed, Kiramat Ali Shah, Naveed Ullah Khan, Bouzid Menaa, Haroon Iqbal, Farid Menaa

“…LNFs were further characterized biologically by in-vitro assessment of their potency against <i>S. aureus</i> clinical strains (<i>N</i> = 16) using the Kirby–Bauer test and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, by <i>ex-vivo</i> assessment to evaluate their cytotoxicity against primary human epidermal keratinocytes using MTT assay, and by in-vivo assessment to estimate their diabetic chronic wound-healing efficiency using NcZ10 diabetic/obese mice (<i>N</i> = 18). Thin and uniform NFs with a smooth surface and standard size (<400 nm) were observed by SEM at the optimized 5:5 (GEL:PVA) volumetric ratio. …”
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Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds by Alsulami KA, Bakr AA, Sirwi A, Elfaky MA, Shaik RA, Alshehri BY, Aodah AH, Al Fayez N, Alshehri AA, Almughem FA, Halwani AA, Tawfik EA

“…The prepared nanofibers were tested in vivo in an S. aureus-infected wound mice model to assess their efficacy, and the results showed that the FA/LDC-PVP had a faster wound closure and the lowest bacterial counts compared to other groups.Conclusion: These findings showed the potential application of the fabricated dual drug-loaded nanofibers as a wound-healing plaster against infected acute wounds.Keywords: electrospinning, nanofibers, fusidic acid, lidocaine, wound healing, infected wound…”
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Dim blue light at night worsens high-fat diet-induced kidney damage via increasing corticosterone levels and modulating the expression of circadian clock genes by Wenji Ren, Zixu Wang, Yulan Dong, Jing Cao, Ting Gao, Qingyun Guo, Yaoxing Chen

“…Nevertheless, the impact of light pollution on kidney disease associated with obesity remains mostly unknown, and potential processes have been minimally investigated. Herein, we fed mice a high-fat diet and gave them dim white (dWL), blue (dBL), green (dGL), and red (dRL) light for 12 weeks. …”
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Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer by Babak Jahangiri, Zahra-Soheila Soheili, Elahe Asadollahi, Mehdi Shamsara, Vahid Shariati, Alireza Zomorodipour

“…The effects of TA-MSCs ontumor volume, survival rate, and lung metastasis were evaluated by subcutaneous co-injection of MSCs with 4T1 cellsin the right flanks of BALB/c mice (n=5 per group). Intratumoral interleukin-12 (IL-12) immunotherapy was performedusing lentiviral particles as a rescue experiment. …”
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Bone Formation by Sheep Stem Cells in an Ectopic Mouse Model: Comparison of Adipose and Bone Marrow Derived Cells and Identification of Donor-Derived Bone by Antibody Staining by Kristian Kjærgaard, Chris H. Dreyer, Nicholas Ditzel, Christina M. Andreasen, Li Chen, Søren P. Sheikh, Søren Overgaard, Ming Ding

“…A-CEAC and BM-CEAC were isolated from five female sheep and seeded on hydroxyapatite granules prior to subcutaneous implantation in immunodeficient mice. The doses of cells in the implants were 0.5 × 106, 1.0 × 106, or 1.5 × 106 A-CEAC and 0.5 × 106 BM-CEAC, respectively. …”
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Exploring the mechanism of rosmarinic acid in the treatment of lung adenocarcinoma based on bioinformatics methods and experimental validation by Chaowang Zhou, Ruqian Zhong, Lei Zhang, Renyi Yang, Yuxin Luo, Huijun Lei, Liang Li, Jianzhong Cao, Zhiying Yuan, Xiaoning Tan, Mengzhou Xie, Haoyu Qu, Zuomei He

“…In vivo experimental results showed that RosA could inhibit the growth of transplanted tumors in nude mice bearing tumors of lung cancer cells, reduce the positive expression of Ki67 in lung tumor tissue, and hinder the proliferation of lung tumor cells. …”
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The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced hypercholesterolemia model by Jan Dusek, Ivana Mejdrová, Klára Dohnalová, Tomas Smutny, Karel Chalupsky, Maria Krutakova, Josef Skoda, Azam Rashidian, Ivona Pavkova, Kryštof Škach, Jana Hricová, Michaela Chocholouskova, Lucie Smutna, Rajamanikkam Kamaraj, Miloš Hroch, Martin Leníček, Stanislav Mičuda, Dirk Pijnenburg, Rinie van Beuningen, Michal Holčapek, Libor Vítek, Magnus Ingelman-Sundberg, Oliver Burk, Thales Kronenberger, Radim Nencka, Petr Pavek

“…Through comprehensive analyses utilizing lipidomics, bile acid metabolomics, and transcriptomics in humanized PXR-CAR-CYP3A4/3A7 mice fed high-fat and high-cholesterol diets, we demonstrate that MI-883 significantly reduces plasma cholesterol levels and enhances fecal bile acid excretion. …”
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Tumor Necrosis Factor Receptor 1 Is Required for Human Umbilical Cord–Derived Mesenchymal Stem Cell–Mediated Rheumatoid Arthritis Therapy by Guangyang Liu, Herui Wang, Chenliang Zhang, Xin Li, Yi Mi, Yaoyao Chen, Liqiang Xu, Li Miao, Haomiao Long, Yongjun Liu

“…Subsequently, we administered hUC-MSCs or siTNFR1-MSCs to DBA/1J male mice with collagen-induced arthritis. The results indicated that hUC-MSCs significantly alleviated the pathological features of RA and suppressed the inflammatory cytokines IFN-γ, TNF-α, and IL-6 in peripheral blood, also in a manner dependent on TNFR1 either. …”
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NUDT21 regulates lysyl oxidase-like 2(LOXL2) to influence ECM protein cross-linking in silica-induced pulmonary fibrosis by Lan Peng, Wenqing Sun, Demin Cheng, Xinying Jia, Wenxiu Lian, Ziwei Li, Haojie Xiong, Ting Wang, Yi Liu, Chunhui Ni

“…By injecting LOXL2 siRNA-loaded liposomes into the tail vein of mice in the silica dust-treated mouse pulmonary fibrosis model, the severity of lung fibrosis was significantly reduced. …”
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Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model by Satoko Kishimoto, Ken-ichi Inoue, Ryoichi Sohma, Shigeru Toyoda, Masashi Sakuma, Teruo Inoue, Ken-ichiro Yoshida

“…Subcutaneous fat from the inguinal region of BALB/c mice was surgically processed (primed) by mincing adipose parenchyma (injury) and ligating the subcutaneous fat-feeding artery (ischemia). …”
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