METTL16 controls airway inflammations in smoking-induced COPD via regulating glutamine metabolism by Xinyu Jia, Shan Liu, Chunan Sun, Manni Zhu, Qi Yuan, Min Wang, Tingting Xu, Zhengxia Wang, Zhongqi Chen, Mao Huang, Ningfei Ji, Mingshun Zhang

“…Moreover, in the Mettl16-deficient (Mettl16+/-) mice challenged with CS, airway inflammation was aggravated. …”
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PfCSP-ferritin nanoparticle malaria vaccine antigen formulated with aluminum-salt and CpG 1018® adjuvants: Preformulation characterization, antigen-adjuvant interactions, and mouse... by John M. Hickey, Nitya Sharma, Max Fairlamb, Jennifer Doering, Yetunde Adewunmi, Katherine Prieto, Giulia Costa, Benjamin Wizel, Elena A. Levashina, Nicholas J. Mantis, Jean-Philippe Julien, Sangeeta B. Joshi, David B. Volkin

“…The in vivo immunological impact of various adjuvanted formulations of the 155 antigen was investigated in mice. The results support the formulation of 155 with AlhydrogelTM + CpG 1018® adjuvants as a promising recombinant malaria vaccine candidate from both a pharmaceutical and immunological perspective.…”
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Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation by Mathias Guayasamin, Lewis R Depaauw-Holt, Ifeoluwa I Adedipe, Ossama Ghenissa, Juliette Vaugeois, Manon Duquenne, Benjamin Rogers, Jade Latraverse-Arquilla, Sarah Peyrard, Anthony Bosson, Ciaran Murphy-Royal

“…Here, using male and female mice we report that early-life stress induces anxiety-like behaviour and fear generalisation in an amygdala-dependent learning and memory task. …”
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Pharmacological Effects of Antioxidant Mycosporine-Glycine in Alleviating Ultraviolet B-Induced Skin Photodamage: Insights from Metabolomic and Transcriptomic Analyses by Kai Wang, Ling Qin, Huan Lin, Mengke Yao, Junhan Cao, Qing Zhang, Changfeng Qu, Yingying He, Jinlai Miao, Ming Liu

“…In this investigation, 30 female ICR mice were divided into four groups: a control group and three Ultraviolet B (UVB)-exposed groups treated with saline or M-Gly via intraperitoneal injection for 30 days. …”
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Effect of Melatonin as an Antioxidant Drug to Reverse Hepatic Steatosis: Experimental Model by Blanca Martínez Soriano, Antonio Güemes, Guillermo Pola, Azucena Gonzalo, Pilar Palacios Gasós, Ana C. Navarro, Roberto Martínez-Beamonte, Jesús Osada, José J. García

“…Melatonin has been successfully tested in experimental models in mice as a drug capable of reversing steatosis. The effect of melatonin on fat metabolism can be summarized as a decrease in lipid peroxidation and a decrease in oxidative stress, biochemical phenomena intimately related to fat deposition in the hepatocyte. …”
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Isolation and function of a human endothelial cell C1q receptor by M. R. Daha, L. Dunn, R. van den Berg, Y. Muizert-de Lange, A. Gerritsen, L. A. van Es

“…Two IgM monoclonal antibodies (mAb) D3 and D5 were raised following immunization of mice with purified receptor preparations. Both monoclonal antibodies increased the binding of 125I-C1q to endothelial cells but F(ab')2 anti-C1qR mAb inhibited the binding of a125I-C1q to EC in a dosedependent manner. …”
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Structural engineering of stabilized, expanded epitope nanoparticle vaccines for HPV by Michaela Helble, Michaela Helble, Xizhou Zhu, Pratik S. Bhojnagarwala, Kevin Liaw, Yangcheng Gao, Amber Kim, Kelly Bayruns, Madison E. McCanna, Joyce Park, Kylie M. Konrath, Kylie M. Konrath, Sam Garfinkle, Sam Garfinkle, Taylor Brysgel, David B. Weiner, David B. Weiner, Daniel W. Kulp, Daniel W. Kulp

“…We demonstrated that many such designs for both the HPV16 E6 and E7 antigens assembled and drove strong CD8+ T-cell responses in mice. We further tested nanoparticles in a genetically diverse, more translationally relevant CD-1 mouse model and demonstrated that both E6 and E7 nanoparticle designs drove a CD8+ biased T-cell response. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Ethanol-induced endometrial injury mouse models were established and H2S donor sodium hydrosulfide (NaHS) was randomly administered to the injured mice. Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. …”
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Liquid-based encapsulation for implantable bioelectronics across broad pH environments by He Sun, Xiaoting Xue, Gabriella L. Robilotto, Xincheng Zhang, ChangHee Son, Xingchi Chen, Yue Cao, Kewang Nan, Yiyuan Yang, Gavin Fennell, Jaewook Jung, Yang Song, Huijie Li, Shao-Hao Lu, Yizhou Liu, Yi Li, Weiyi Zhang, Jie He, Xueju Wang, Yan Li, Aaron D. Mickle, Yi Zhang

“…We also demonstrate the in vivo biocompatibility of our encapsulation approach and show that encapsulated wireless optoelectronics maintain robust operation throughout 3 months of implantation in freely moving mice. These results indicate that our encapsulation strategy has the potential to protect implantable bioelectronic devices in a wide range of research and clinical applications.…”
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NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity by Rui Ma, Zhenlong Li, Hejun Tang, Xiaojin Wu, Lei Tian, Zahir Shah, Ningyuan Liu, Tasha Barr, Jianying Zhang, Sean Wang, Srividya Swaminathan, Guido Marcucci, Yong Peng, Michael A. Caligiuri, Jianhua Yu

“…This can be reversed by injecting NKp46+ ILC1s into NKp46 knock-out mice. Human NKp46+ ILC1s exhibit stronger cytokine production and cytotoxicity than their NKp46− counterparts, suggesting that NKp46 plays a similar role in humans. …”
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Integrative Bioinformatics Analysis to Identify Key Ferroptosis-Related Genes and Immune Infiltration in Aortic Aneurysm and Dissection: Implication of PTGS2 by An W, Luo J, Zhang C, Xiao Q

“…Functionally, administration of celecoxib, a PTGS2-specific inhibitor, into mice significantly reduced β-aminopropionitrile-induced AAD development and progression.Conclusion: Through an integrative bioinformatics analysis, we have identified multiple key AAD-related FRGs including PTGS2. …”
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Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds by Jun Tan, Danshu Wang, Yu Lu, Yehan Wang, Zongcai Tu, Tao Yuan, Lu Zhang

“…The 40 % ethanol eluting fraction of 40EUS showed the strongest a-glucosidase inhibition with the IC50 value of 37.79 ng/mL, it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice. Twenty-six compounds were identified from 40 % ethanol fraction by using HPLC-QTOF-MS/MS, hydrolysable tannins (including 11 ellagitannins and 4 gallotannins) were the major compounds, phenolic acids came to the second. …”
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Adverse outcome pathway-based assessment of pulmonary toxicity from the in vivo mixture of biocides dinotefuran and cetylpyridinium chloride by Yoon Cho, Mi-Kyung Song, Dong Im Kim, Min-Seok Kim, Kyuhong Lee

“…In this study, we aimed to validate these AOPs by assessing the toxicity of cetylpyridinium chloride and dinotefuran mixture. Sixty C57BL/6 male mice were exposed to either dinotefuran or cetylpyridinium chloride or a mixture of the two via intratracheal instillation (ITI) to examine the synergistic effects of MIE and key events (KEs) within putative AOPs. …”
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Phenylalanine-derived β-lactam TRPM8 antagonists: revisiting configuration and new benzoyl derivatives by Cristina Martín-Escura, Maria Angeles Bonache, Alicia Medina-Peris, Thomas Voets, Antonio Ferrer-Montiel, Asia Fernández-Carvajal, Rosario González-Muñiz

“…The pharmacological activity in vivo was studied on a mice model of oxaliplatin-induced peripheral neuropathy. …”
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Gender-Specific Hippocampal Dysrhythmia and Aberrant Hippocampal and Cortical Excitability in the APPswePS1dE9 Model of Alzheimer’s Disease by Anna Papazoglou, Julien Soos, Andreas Lundt, Carola Wormuth, Varun Raj Ginde, Ralf Müller, Christina Henseler, Karl Broich, Kan Xie, Dan Ehninger, Britta Haenisch, Marco Weiergräber

“…Automatic seizure detection revealed severe electroencephalographic seizure activity in both M1 and CA1 deflection in APPswePS1dE9 mice with gender-specific characteristics. Frequency analysis of both surface and deep EEG recordings elicited complex age, gender, and activity dependent alterations in the theta and gamma range. …”
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Microglia TRPC1 SUMOylation drives neuroinflammation after stroke by modulating NLRP3 activity via increasing TRPC1 interaction with ARRB2 by Huinan Zhang, Xinzhe Du, Tian Gao, Xing Wang, Huifeng Zhang, Manyang Yu, Jing Huang

“…Our findings suggest a notable increase in microglial TRPC1 SUMOylation within both the middle cerebral artery occlusion reperfusion (MCAO/R) model and the in vitro oxygen-glucose deprivation/regeneration model. Mice with a loss of TRPC1 SUMOylation in microglia exhibited improved stroke outcomes including reduced behavior deficits, infarct volume, blood brain barrier damage as well as neuronal apoptosis. …”
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The regulatory role of cystatin C in autophagy and neurodegeneration by T. A. Korolenko, A. B. Shintyapina, A. B. Pupyshev, A. A. Akopyan, G. S. Russkikh, M. A. Dikovskaya, V. A. Vavilin, E. L Zavjalov, M. A. Tikhonova, T. G. Amstislavskaya

“…In an experiment on C57Bl/6J mice, cystatin C concentration was significantly higher in brain tissue than in the liver and spleen: an indication of an important function of this cysteine protease inhibitor in the brain. …”
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Dihydrotanshinone I enhanced BRAF mutant melanoma treatment efficacy by inhibiting the STAT3/SOX2 signaling pathway by Xing Luo, Yi Duan, Jinwei He, CongGai Huang, Jun Liu, Yifan Liu, Mengdei Xu, Qiong Dai, Zhihui Yang

“…CCK-8 was used to assess cell viability, while flow cytometry was used to identify apoptosis. In addition, mice were inoculated with A375 cells to establish a model of tumour formation, and various drug groups and treatment models were utilized. …”
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Quercetin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy by Inhibiting ERK/NF-κB Pathway by Liwei Wu, Qinghui Zhang, Weiqi Dai, Sainan Li, Jiao Feng, Jingjing Li, Tong Liu, Shizan Xu, Wenwen Wang, Xiya Lu, Qiang Yu, Kan Chen, Yujing Xia, Jie Lu, Yingqun Zhou, Xiaoming Fan, Chuanyong Guo

“…The aim of the present study was to determine the protective effect of quercetin (QE) on hepatic IR injury via the ERK/NF-κB pathway. Methods. Mice were randomized into the sham, IR, QE100 + IR, and QE200 + IR groups. …”
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Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon by Menglu Chen, Lei Gao, Pan Chen, Dandan Feng, Yalin Jiang, Yongchao Chang, Jianjun Jin, Fong-Fong Chu, Qiang Gao

“…Materials and Methods. C57BL/6J (B6) mice were treated with either low-dose (1.0 mg/kg) or high-dose (2.0 mg/kg) 5-HT by enema, low-dose (1.0%) or high-dose (2.5%) DSS, or combined low-dose (1.0%) DSS and (1.0 mg/kg) 5-HT. …”
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