Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Methods We employed microarray and single-cell RNA sequencing (scRNA-seq) to investigate gene expression changes and immune cell dynamics in mice following crush injury at 3 and 7 days post-injury (dpi). …”
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A molecular toxicological study to explore potential health risks associated with ultrafine particle exposure in cold and humid indoor environments by Ziyu Shu, Shuo Qing, Xu Yang, Ping Ma, Yang Wu, Baizhan Li, Fangxin Fang, Runming Yao

“…Through comprehensive lung function tests, histopathological examinations, and biomarker analyses, this research focuses on the modulation of oxidative stress signaling pathways and NF-κB activation. Male Balb/c mice were exposed to specific concentrations of CB-NPs (30–50 nm in diameter, 0.184 mg/(kg·day)) in a controlled environmental chamber mimicking cold (10°C/14°C) and humid (90 % RH) conditions over three weeks. …”
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Stimulus-activated ribonuclease targeting chimeras for tumor microenvironment activated cancer therapy by Yuqi Zhang, Jinfeng Zhu, Ling Qiu, Zhengzhong Lv, Zhongsheng Zhao, Xingxiang Ren, Yirui Guo, Yan Chen, Miao Li, Yurong Fan, Zhixin Han, Yiming Feng, Haibin Shi

“…The degradation of pre-miR-21 by TaRiboTACs significantly increases the radiotherapeutic susceptibility of cancer cells achieving efficient suppression of human lung adenocarcinoma A549 tumors in living mice.…”
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Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses by Zhenwei Liu, Yuting Xian, Jixian Lan, Zhichao Zhou, Xiao Li, Rong Zhou, Dehui Chen, Xingui Tian

“…We found that the knob proteins elicited robust binding and neutralizing antibody responses after three immunizations of mice. In addition, mouse antisera raised against the knob proteins exhibited cross-neutralizing activity against original species B members. …”
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Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes by Maima Matin, Natalia Ksepka, Kamil Wysocki, Michel-Edwar Mickael, Alpana Pande, Jarosław Olav Horbańczuk, Artur Jóźwik, Olga Adamska, Artur Stolarczyk, Laszlo Barna Iantovics, Yordanka Ilieva, Pencho Penchev, Atanas G. Atanasov

“…Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. …”
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Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model by Agnieszka Szuster-Ciesielska, Leszek Wawiórka, Dawid Krokowski, Nikodem Grankowski, Łukasz Jarosz, Urszula Lisiecka, Marek Tchórzewski

“…An in vitro exposure of spleen lymphocytes from the immunized mice especially to the P1, P2, and P0-(P1-P2)2 protein caused 2-3-fold higher cell proliferation than that in the case of lymphocytes from the nonimmunized animals, suggesting development of immune memory. …”
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Combination Fractional Carbon Dioxide Laser Treatment and Bone Marrow Mesenchymal Stem Cell Therapy Enhances the Treatment of Skin Photoaging in a Murine Model System by Li L, He Z, Yu C, Zhang C, Yu Y, Li Y, Xu X

“…Li Li,1– 3,* Zeyu He,3– 5,* Chengqian Yu,3– 5 Chao Zhang,3– 5 Yanqiu Yu,6 Yuanhong Li,3– 5 Xuegang Xu3– 5 1ShanXi Medical University, Taiyuan, People’s Republic of China; 2Department of Dermatology, The First Hospital of ShanXi Medical University, Taiyuan, 030001, People’s Republic of China; 3Department of Dermatology, The First Hospital of China Medical University, Shenyang, 030001, People’s Republic of China; 4Key Laboratory of Immunodermatology (China Medical University), Ministry of Education, Shenyang, 110001, People’s Republic of China; 5NHC Key Laboratory of Immunodermatology (China Medical University), Shenyang, 110001, People’s Republic of China; 6Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang, 110122, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xuegang Xu, Department of Dermatology, The First Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning Province, 110001, People’s Republic of China, Email xuxuegang2749290@163.com Li Li, Department of Dermatology, The First Hospital of ShanXi Medical University, No. 85 South Jiefang Road, Yingze District, Taiyuan, Shanxi Province, 030001, People’s Republic of China, Email lilizaimu0807@163.comBackground: Fractional carbon dioxide lasers and bone marrow mesenchymal stem cells (BMSCs) are commonly employed in the treatment of skin photoaging.Objective: This study was developed to explore the effects of combination carbon dioxide laser treatment and BMSC injection on skin photoaging and the underlying molecular mechanisms.Methods & Materials: In total, 24 mice with experimentally photoaged skin were separated into control, carbon dioxide fractional laser treatment, combination therapy, and BMSC injection groups. …”
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Germinal Center B Cells are Uniquely Targeted by Antibody-Suppressor CXCR5+CD8+ T Cells by Jason M. Zimmerer, PhD, Sachi Chaudhari, BS, Kavya Koneru, BS, Jing L. Han, MD, PhD, Mahmoud Abdel-Rasoul, MA, Hope Uwase, MA, Tai Yi, MD, Christopher K. Breuer, MD, Ginny L. Bumgardner, MD, PhD

“…In vivo CD8-mediated cytotoxicity to wild-type germinal center (GC) B cells or wild-type CD4+ T follicular helper cells (TFH cells) was assessed in RAG1 knockout mice. The impact of in vivo adoptive transfer of CD8+ TAb-supp cells into hepatocyte or kidney transplant recipients on the quantity of lymphoid immune-cell subsets was assessed. …”
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Neuroligin 1 Regulates Autistic‐Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor‐Expressing Medium Spiny Neurons by Dandan Lv, An Liu, Ziyue Yi, Mingdao Mu, Miao Wu, Xingcan Li, Kun Cao, Ruining Liu, Zhengping Jia, Junhai Han, Wei Xie

“…Finally, through single‐nucleus RNA sequencing (sn‐RNAseq) and protein detection verification, it is revealed that the overactivation of protein kinase C (PKC) in Nlgn1‐deficient mice contributes to excessive repetitive behaviors and increased neuronal excitability. …”
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WT1 Peptide Cancer Vaccine for Patients with Hematopoietic Malignancies and Solid Cancers by Yoshihiro Oka, Akihiro Tsuboi, Olga A. Elisseeva, Hiroko Nakajima, Fumihiro Fujiki, Manabu Kawakami, Toshiaki Shirakata, Sumiyuki Nishida, Naoki Hosen, Yusuke Oji, Ichiro Kawase, Haruo Sugiyama

“…It was also demonstrated that mice immunized with the WT1 peptide rejected challenges by WT1-expressing cancer cells and survived with no signs of autoaggression to normal organs that physiologically expressed WT1. …”
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Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential by Jasbir Kour, Bashir Ahmad Lone, Amit Kumar, Bashir A. Ganai, Govind Yadav, Prasoon Gupta, Md.Niamat Ali, Seema Akbar

“…Methods: The methanol crude extract and its subsequent fractions were tested for antimutagenic/antigenotoxic activity in vitro (Ames assay) and in vivo (Chromosomal aberration assay in Balb/c mice). The active (ethyl acetate) fraction was further investigated to isolate and identify its bioactive compounds utilizing chromatographic purification and detailed spectroscopic techniques respectively. …”
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The discovery and characterization of K‐563, a novel inhibitor of the Keap1/Nrf2 pathway produced by Streptomyces sp by Ran Hori, Kozo Yamaguchi, Hidetaka Sato, Miwa Watanabe, Kyoko Tsutsumi, Susumu Iwamoto, Masayuki Abe, Hideyuki Onodera, Satoshi Nakamura, Ryuichiro Nakai

“…K‐563 also exerted synergistic combinational effects with lung cancer chemotherapeutic agents. An in vivo study in mice xenotransplanted with A549 cells to further explore the therapeutic potential of K‐563 revealed that it also inhibited Keap1/Nrf2 pathway in lung cancer tumors. …”
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mtSTAT3 suppresses rheumatoid arthritis by regulating Th17 and synovial fibroblast inflammatory cell death with IL-17-mediated autophagy dysfunction by Seon-Yeong Lee, Jeonghyeon Moon, A Ram Lee, Young-Mee Moon, Jeong Won Choi, Chae Rim Lee, Su Been Jeon, Hee Su Sohn, Jeehee Youn, Dongyun Shin, Sung-Hwan Park, Mi-La Cho

“…The abnormal accumulation of autophagosomes and the expression of inflammatory cell death factors were also decreased in these mice. In mouse and human immune cells, ZnSO4, an inducer of mitochondrial STAT3, decreases the production of reactive oxygen species, the IL-17 concentration, and differentiation into Th17 cells. …”
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High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression by Zhengyi Wu, Chao Li, Shouhua Zhang, Liang Sun, Junwen Hu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Tianxiang Chen, Kai Wang, Xiangbao Yin, Jinlong Yan

“…In vitro experiments replicated the HIFP environment to observe changes in HCC cell behavior and protein expression, while in vivo studies involved mice portal hypertension to create an orthotopic liver cancer model, allowing for the evaluation of tumor progression. …”
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Protection of Fecal Microbiota Transplantation in a Mouse Model of Multiple Sclerosis by Kanglan Li, Shouchao Wei, Li Hu, Xiaojian Yin, Yingren Mai, Chunmei Jiang, Xiaoping Peng, Xingxing Cao, Zhongkai Huang, Haihong Zhou, Guoda Ma, Zhou Liu, Huiliang Li, Bin Zhao

“…To evaluate the viability of FMT for MS treatment and its impact on MS pathology, we tested FMT in mice with experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. …”
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TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p by Huikang Xu, Jiamin Fu, Lijun Chen, Sining Zhou, Yangxin Fang, Qi Zhang, Xin Chen, Li Yuan, Yifei Li, Zhenyu Xu, Charlie Xiang

“…The small EVs secreted by TNF-α-stimulating MenSCs were more effective in colonic mice than those secreted directly by MenSCs, as evidenced by the results of histopathology analysis of colonic tissue, immunohistochemistry for tight junction proteins, and enzyme-linked immunosorbent assay (ELISA) for cytokine expression profiles in vivo. …”
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PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation by Yaoxu Li, Bing Li, Kai Yang, Lihua Zhu, Hong Tang, Yinpei Huang, Jinhai Deng, Jun Duan

“…Decitabine combined with LW6 upregulated PER3, downregulated HIF-1α, and inhibited lymph node metastasis of OSCC in nude mice. Our findings reveal the role and mechanism of HIF-1α regulation by PER3 and support the potential clinical application of targeting PER3 in treating OSCC metastasis.…”
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Impact of Timing the Combination of Radiotherapy and PD-1 Inhibitors on Outcomes in Patients with Hepatocellular Carcinoma by Zhong L, Peng W, Sun J, Luo Y, Sheng H, Wu Y, Zhou T, Zhou C, Cao C

“…Additionally, we created an experimental tumor model using 6-week-old female mice to validate our findings.Results: In the concurrent group, the median overall survival was indefinite; however, it was 13 months in the sequential group (95% confidence interval [CI] 6.7– 19.3 months, P=0.010). …”
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Stevioside mitigates metabolic dysregulation in offspring induced by maternal high-fat diet: the role of gut microbiota-driven thermogenesis by Jin Ye, Renjie Shi, Xiaoning Wu, Hua Fan, Yapei Zhao, Xinyun Hu, Lulu Wang, Xiaowei Bo, Dongning Li, Yunshu Ge, Danna Wang, Bing Xia, Zhenting Zhao, Chunxia Xiao, Beita Zhao, Yutang Wang, Xuebo Liu

“…Our study found that maternal STV supplementation reduced obesity during pregnancy, decreased abnormal lipid accumulation in offspring mice caused by maternal obesity, and modified the gut microbiota of both dams and offspring, notably increasing the abundance of Lactobacillus apodemi (L. apodemi). …”
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Ursodeoxycholic acid protects against sepsis-induced acute kidney injury by activating Nrf2/HO-1 and inhibiting NF-κB pathway by Yunpeng Lou, Hongguang Shi, Ning Sha, Feifei Li, Xiaofeng Gu, Huiyan Lin

“…Methods Sixty male C57BL/6 N mice were utilized to establish a sepsis-induced AKI model through intravenous injection of lipopolysaccharides (LPS, 10 mg/kg). …”
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