SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma ce... by Tianci Han, Xiaoqi Guo, Junwei Xie, Wei Tong, Liang Zhang

“…Overexpression of ETV1 promoted ESCC cell proliferation in vitro and xenograft tumor growth in nude mice, while ETV1 knockdown elicited the opposite effects. …”
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Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway by Chen Feng, Wei Mao, Chenyang Yuan, Pin Dong, Yuying Liu

“…Tumor formation assays are performed in nude mice using sh-CHRNA5 Cal27 cells, followed by western blot and immunohistochemical staining. …”
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EMC2 suppresses ferroptosis via regulating TFRC in nasopharyngeal carcinoma by Xianghui Chen, Xiaoyan Wang, Yuxia Zou, Yan Wang, Tingting Duan, Zijie Zhou, Yi Huang, Qing Ye

“…In addition, EMC2 knockdown suppressed xenograft tumor growth and enhanced ferroptosis in nude mice. Mechanistically, we identified transferrin receptor (TFRC) as a critical downstream protein. …”
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Preclinical optimization of a GPC2-targeting CAR T-cell therapy for neuroblastoma by Nan Li, Haiying Qin, Rosa Nguyen, Mitchell Ho, Ming Sun, Eytan Ruppin, Michael C Kelly, Yingying Cao, Reona Okada, Jeyshka M Reyes-González, Hannah G Stack, Charlie Seibert, Carol J Thiele

“…We developed a new chimeric antigen receptor (CAR) targeting glypican-2 (GPC2) and conducted iterative preclinical engineering of the CAR structure to maximize its anti-tumor efficacy before clinical translation.Methods We evaluated different GPC2-CAR constructs by measuring the CAR activity in vitro. NOD-SCID mice engrafted orthotopically with human NB cell lines or patient-derived xenografts and treated with human CAR T cells served as in vivo models. …”
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C5a-C5aR1 axis mediates lung inflammation and fibrosis induced by single-walled carbon nanotubes via promoting neutrophils recruitment by Jiaojiao Zhu, Xiang Zhang, Lanlan Li, Hongxu Yang, Hang Liu, Danyang Wu, Zikai Liu, Bin Liu, Tong Shen

“…Here, we established a mice model of SWCNT-induced lung injury by intratracheal instillation and found that C5a-C5a receptor-1 (C5aR1) signaling was significantly activated along with abundant neutrophils recruitment in lungs at early phase post SWCNT administration, which were positively correlated with early lung inflammation and late pulmonary fibrosis. …”
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Human striatal progenitor cells that contain inducible safeguards and overexpress BDNF rescue Huntington’s disease phenotypes by Danielle A. Simmons, Sridhar Selvaraj, Tingshuo Chen, Gloria Cao, Talita Souto Camelo, Tyne L.M. McHugh, Selena Gonzalez, Renata M. Martin, Juste Simanauskaite, Nobuko Uchida, Matthew H. Porteus, Frank M. Longo

“…This study examined the efficacy of intrastriatally transplanted striatal progenitor cells (STRpcs) derived from these hPSCs against HD phenotypes in R6/2 mice. Engrafted STRpcs overexpressing BDNF alleviated motor and cognitive deficits and reduced mutant huntingtin aggregates. …”
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RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection by Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Fuminori Sugihara, Yasuhiko Horiguchi

“…Our results show that melanin confers a survival advantage to B. parapertussis within human macrophages through its protective effect against reactive oxygen species and eventually contributes to respiratory infection of the bacterium in mice. This study proposes melanin as a virulence factor involved in the increased survival of B. parapertussis during host infection.…”
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Mechanism of melanoma suppression by Prosopis juliflora derived alkaloids: Apoptosis induction and signaling pathway inhibition by Jasoda Choudhari, Snehal K. Nimal, Shridhar Chougule, Trupti Shinde, N.R. Dhatrak, Gopal C. Kundu, Rajesh N. Gacche

“…Finally, anti-tumor activity of PJLME-AF was further confirmed in a in-vivo mice model. Additionally, PJLME-AF combined with dacarbazine, exhibited enhanced cytotoxicity on melanoma compared to the drug alone. …”
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Preclinical evaluation of avutometinib and defactinib in high‐grade endometrioid endometrial cancer by Tobias Max Philipp Hartwich, Miranda Mansolf, Cem Demirkiran, Michelle Greenman, Stefania Bellone, Blair McNamara, Shuvro P. Nandi, Ludmil B. Alexandrov, Yang Yang‐Hartwich, Silvia Coma, Jonathan Pachter, Alessandro D. Santin

“…Mechanistic studies were performed using Western blot assays while in vivo experiments were completed in UTE10 engrafted mice treated with either vehicle, avutometinib, VS‐4718, or their combination through oral gavage. …”
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Plant-nanoparticles enhance anti-PD-L1 efficacy by shaping human commensal microbiota metabolites by Yun Teng, Chao Luo, Xiaolan Qiu, Jingyao Mu, Mukesh K. Sriwastva, Qingbo Xu, Minmin Liu, Xin Hu, Fangyi Xu, Lifeng Zhang, Juw Won Park, Jae Yeon Hwang, Maiying Kong, Zhanxu Liu, Xiang Zhang, Raobo Xu, Jun Yan, Michael L. Merchant, Craig J. McClain, Huang-Ge Zhang

“…Colonization of germ-free male mice with gut bacteria from anti-PD-L1 non-responding patients supplemented with DHA enhances the efficacy of anti-PD-L1 therapy compared to controls. …”
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The GLP-1R agonist semaglutide reshapes pancreatic cancer associated fibroblasts reducing collagen proline hydroxylation and favoring T lymphocyte infiltration by Chiara Cencioni, Silvia Malatesta, Virginia Vigiano Benedetti, Valerio Licursi, Livia Perfetto, Federica Conte, Danilo Ranieri, Armando Bartolazzi, Martina Kunkl, Loretta Tuosto, Alberto Larghi, Geny Piro, Antonio Agostini, Giampaolo Tortora, Vincenzo Corbo, Carmine Carbone, Francesco Spallotta

“…Methods PDAC development upon dysmetabolic conditions was investigated in: 1) high fat diet fed wild type immunocompetent syngeneic mice by orthotopic transplantation of pancreatic intraepithelial neoplasia (PanIN) organoids; and 2) primary pancreatic CAFs isolated from chemotherapy naïve PDAC patients with/without an history of metabolic syndrome. …”
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High-starchy carbohydrate diet aggravates NAFLD by increasing fatty acids influx mediated by NOX2 by Yuqi Gao, Rui Hua, Kezheng Peng, Yuemiao Yin, Chenye Zeng, Yannan Guo, Yida Wang, Liyuan Li, Xue Li, Ying Qiu, Zhao Wang

“…Our study showed that a high-starch carbohydrate diet (HCD) led to the occurrence and exacerbation of NAFLD in mice. Transcriptomics and metabonomic analyses showed that the increased fatty acid influx mediated by NADPH oxidase 2 (NOX2) exacerbated NAFLD. …”
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Universal conditional networks (UniCoN) for multi-age embryonic cartilage segmentation with Sparsely annotated data by Nishchal Sapkota, Yejia Zhang, Zihao Zhao, Maria Jose Gomez, Yuhan Hsi, Jordan A. Wilson, Kazuhiko Kawasaki, Greg Holmes, Meng Wu, Ethylin Wang Jabs, Joan T. Richtsmeier, Susan M. Motch Perrine, Danny Z. Chen

“…Current research on this disease using mouse models faces challenges since it involves accurately segmenting (precisely delineating) the developing cartilage in 3D micro-CT images of embryonic mice. Tackling this segmentation task with deep learning (DL) methods is laborious due to the big burden of manual image annotation, expensive due to the high acquisition costs of 3D micro-CT images, and difficult due to embryonic cartilage’s complex and rapidly changing shapes. …”
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YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment by Zhenyun Yang, Xin Wang, Yizhen Fu, Weijie Wu, Zili Hu, Qingyang Lin, Wei Peng, Yangxun Pan, Juncheng Wang, Jinbin Chen, Dandan Hu, Zhongguo Zhou, Li Xu, Yaojun Zhang, Jiajie Hou, Minshan Chen

“…Studies using tumor-bearing liver-specific Ythdf2 knockout mice revealed that hepatocyte YTHDF2 suppresses liver tumor growth through CD8+ T cell recruitment and activation. …”
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner I... by Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach

“…This study was designed to determine if human umbilical cord perivascular cells modulate tumor growth when injected systemically in a tumor-bearing mouse model. Immunodeficient mice-bearing palpable subcutaneous SK-MEL-28 human melanoma tumors were randomized to receive a tail vein injection of three human umbilical cord perivascular cell lines resuspended in hank’s buffer saline solution (vehicle) or vehicle only, as a control. …”
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The active roles of Rhodotorula mucilaginosa ZTHY2 in regulating antioxidant capacity and immune function of Leizhou black ducks by Jiang Wu, Yingxin Hu, Namula Zhao, Wei Yang, Zhibao Chen, Zhibao Chen

“…Previous studies in mice have demonstrated that Rhodotorula mucilaginosa ZTHY2 can promote animal growth, enhance antioxidant and immune functions, and regulate intestinal flora in our laboratory. …”
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Changes induced in mouse lipid metabolism by simultaneous impact of antisense oligonucleotide derivatives to <i>apoB</i>, <i>PCSK9</i>, and <i>apoCIII</i> mRNAs by S. I. Oshevski, Y. I. Ragino, E. V. Kashtanova, Y. V. Polonskaya, E. M. Stakhneva, V. P. Nikolin, N. A. Popova, N. A. Kolchanov, M. I. Voevoda

“…The goal of the work was to assess the changes in the lipid metabolism caused by variants of joint impact of the ASOs targeted to the mRNAs of its key genes: apoB, PCSK9, and apoCIII. Female C57BL/6J mice; nuclease-protected 13- and 20-nucleotide ASOs, and standard protocols for quantification of lipoproteins (HDL CHL, non-HDL CHL, and total CHL) and ALT in the blood serum were used in the work. …”
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Characterization of the Beekeeping Value Chain: Challenges, Perceptions, Limitations, and Opportunities for Beekeepers in Kayonza District, Rwanda by Borden Mushonga, Landouard Hategekimana, Gervais Habarugira, Erick Kandiwa, Alaster Samkange, Basiamisi Victor Ernest Segwagwe

“…The hive pests encountered were hive beetles (25%), small black ants (19%), wax moths (12%), lizards (8%), termites (7%), birds (6%), mites (3%), and mice (2%). Fifty-five percent of the respondents hung their hives on trees to prevent pest invasion with 95% effectiveness, 25% frequently smoked their hives with 85% effectiveness, 16% cleared the bushes around apiaries with 55% effectiveness, and 4% used traditional plant insect repellents with 35% effectiveness. …”
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Diabetic Nephropathy Induced by Increased Ace Gene Dosage Is Associated with High Renal Levels of Angiotensin (1–7) and Bradykinin by Nádia Bertoncello, Roseli Peres Moreira, Danielle Yuri Arita, Danielle S. Aragão, Ingrid Kazue Mizuno Watanabe, Patricia S. Dantas, Ralmony Santos, Rodolfo Mattar-Rosa, Rodrigo Yokota, Tatiana Sousa Cunha, Dulce Elena Casarini

“…Population studies have shown an association between diabetic nephropathy (DN) and insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) gene (ACE in humans, Ace in mice). The aim was to evaluate the modulation of Ace copies number and diabetes mellitus (DM) on renal RAS and correlate it with indicators of kidney function. …”
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A multifunctional mesoporous silica drug delivery nanosystem that ameliorates tumor hypoxia and increases radiotherapy efficacy by Yanhong Chu, LiFeng Wang, Yaohua Ke, Xiaoyu Feng, Wenmei Rao, Wei Ren, Kai Xin, Yan Wang, Lixia Yu, Baorui Liu, Qin Liu

“…Tumor cells treated with RT combined with these nanoparticles displayed reduced oxidative stress, alleviated hypoxia, and normalized blood vessel formation. Notably, all mice in the RT + PD-1 + MSN@R837-Mn/ZnO2 group achieved complete tumor regression with extended survival. …”
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