Single domain antibody-scFv conjugate targeting amyloid β and TfR penetrates the blood–brain barrier and interacts with amyloid β by Rebecca Faresjö, Elisabet O. Sjöström, Tiffany Dallas, Magnus M. Berglund, Jonas Eriksson, Dag Sehlin, Stina Syvänen

“…The VHH-scFv fusions were further investigated in mouse models with Aβ pathology, showing higher retention compared to wild-type mice without Aβ pathology. Our findings suggest that these novel VHH-based FPs hold potential for therapeutic and diagnostic applications in AD, providing a strategy to overcome BBB limitations and enhance brain targeting of antibody-based treatments. …”
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Infected burn wound healing using Hydroxy-propyl-methyl cellulose gel containing bacteriophages against Pseudomonas aeruginosa and Klebsiella pneumoniae by Meysam Adibi, Kazem Javanmardi, Noor Al-Huda Ali A.H. Saeed, Mohammad Ali Mobasher, Javad Jokar, Abdolmajid Ghasemian, Niloofar Rahimian, Ava Soltani Hekmat

“…The infectious burn wound model was performed on Balb/C male mice, aged six weeks and weighing 25 ± 5 gr. The effects of the Hydroxy-propyl-methyl cellulose (HPMC) gel containing phage were investigated compared to gentamicin. …”
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A detoxified TLR4 agonist inhibits tumour growth and lung metastasis of osteosarcoma by promoting CD8+ cytotoxic lymphocyte infiltration by Ryunosuke Oyama, Akira Nabeshima, Makoto Endo, Alexey Novikov, Toshifumi Fujiwara, Capucine Phelip, Nobuhiko Yokoyama, Yoshinao Oda, Martine Caroff, Yoshihiro Matsumoto, Jerome Kerzerho, Yasuharu Nakashima

“…We evaluated tumour growth, lung metastases, and immune cell infiltration in wild-type and TLR4-mutant mice and performed selective immunodepletion. Results Lipo-MP-LPS exhibited antitumour effects against localised osteosarcoma tumours and lung metastases, like those of natural LPS. …”
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A current review on animal models of anti-asthmatic drugs screening by Shivam Singh, Sunita Kularia, Shivakshi Shukla, Mithilesh Singh, Manish Kumar, Ashish Kumar Sharma

“…In-Vivo Models: In vivo small animal models, Broncho Spasmolytic Activity in anaesthetized Guinea Pigs, Guinea Pigs Respiratory and Vascular Dysfunction Caused by Arachidonic Acid or platelet-activated factor (PAF), Guinea Pig Asphyxia Induced by Serotonin Aerosol and Anaphylactic Microshock, Guinea Pigs Under Anaesthesia: Histamine-Induced Bronchoconstriction, Microshock in Rabbits and Pneumotachography in Guinea Pigs, Guinea Pig Bronchial Hyperactivity, Guinea Pig Airway Microvascular Leakage, Mice With Inflammatory Airways. Conclusion: This review focusses on the benefits and limitations of current animal models in asthma research, emphasising the need for more sophisticated, predictive models to decrease translational failures. …”
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Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota by Ruting Zhong, Lanbin Chen, Yuanyuan Liu, Shouxia Xie, Sumei Li, Bin Liu, Chao Zhao

“…Additionally, it diminished the weight loss, reduced fasting blood glucose levels and hemoglobin A1c activity, and promoted glucose tolerance and fasting serum insulin activity in T2D mice. Histopathological analysis of the liver indicated hepatic protection by aloin. …”
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Liraglutide Attenuates Nonalcoholic Fatty Liver Disease through Adjusting Lipid Metabolism via SHP1/AMPK Signaling Pathway by Peng Yu, Xi Xu, Jing Zhang, Xuan Xia, Fen Xu, Jianping Weng, Xiaoyang Lai, Yunfeng Shen

“…The effects of LR were examined on the high-fat diet-induced in vivo model in mice and in vitro model of NAFLD in human HepG2 cells. …”
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Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model by Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji

“…First preserved at −20°C for molecular analysis whereas the second is used for experimental infection in mice. Parasite DNA was extracted, and AgB2 genome was amplified and sequenced. …”
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Differential T cell accumulation within intracranial and subcutaneous melanomas is associated with differences in intratumoral myeloid cells by Katarzyna Stasiak, Aaron D. Stevens, Ashley C. Bolte, Colleen T. Curley, Mirna Perusina Lanfranca, Robin S. Lindsay, Ukpong B. Eyo, John R. Lukens, Richard J. Price, Timothy N. J. Bullock, Victor H. Engelhard

“…Surprisingly, we found no evidence for T cell activation in draining lymph nodes of SC or IC tumor-bearing mice, consistent with the fact that dendritic cells (DC) that had acquired tumor antigen showed an immature phenotype. …”
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Expression and Function of the Homeostatic Molecule Del-1 in Endothelial Cells and the Periodontal Tissue by Jieun Shin, Kavita B. Hosur, Kalyani Pyaram, Ravi Jotwani, Shuang Liang, Triantafyllos Chavakis, George Hajishengallis

“…Developmental endothelial locus-1 (Del-1) is an endothelial cell-secreted protein that limits the recruitment of neutrophils by antagonizing the interaction between the LFA-1 integrin on neutrophils and the intercellular adhesion molecule (ICAM)-1 on endothelial cells. Mice with genetic or age-associated Del-1 deficiency exhibit increased neutrophil infiltration in the periodontium resulting in inflammatory bone loss. …”
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Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis by Feiyan Zhao, Wei Liu, Shaojie Yue, Lei Yang, Qingzhong Hua, Yan Zhou, Haipeng Cheng, Ziqiang Luo, Siyuan Tang

“…The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay. …”
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Prioritization of genes associated with the pathogenesis of leukosis in cattle by N. S. Yudin, N. L. Podkolodnyy, T. A. Agarkova, E. V. Ignatieva

“…The following criteria were used to prioritize 446 genes from the catalog: (1) the gene is associated with leukemia according to a genome-wide association study; (2) the gene is associated with leukemia according to a case-control study; (3) the role of the gene in leukemia development has been studied using knockout mice; (4) protein-protein interactions exist between the gene-encoded protein and either viral particles or individual viral proteins; (5) the gene is annotated with Gene Ontology terms that are overrepresented for a given list of genes; (6) the gene participates in biological pathways from the KEGG or REACTOME databases, which are over-represented for a given list of genes; (7) the protein encoded by the gene has a high number of protein-protein interactions with proteins encoded by other genes from the catalog. …”
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Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse by Chunjun Sheng, Feng Li, Ziwei Lin, Manna Zhang, Peng Yang, Le Bu, Hui Sheng, Hong Li, Shen Qu

“…Our results showed that chronic euglycemia was maintained in the db/db mice with long-term CR intervention, and β-cell dedifferentiation was significantly reduced. …”
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Inhibiting C-Reactive Protein for the Treatment of Cardiovascular Disease: Promising Evidence from Rodent Models by Alexander J. Szalai, Mark A. McCrory, Dongqi Xing, Fadi G. Hage, Andrew Miller, Suzanne Oparil, Yiu-Fai Chen, Michelle Mazzone, Richard Early, Scott P. Henry, Thomas A. Zanardi, Mark J. Graham, Rosanne M. Crooke

“…Likewise in human CRP transgenic mice treated for 2 weeks with a human CRP-specific ASO, blood human CRP was reduced by >70% and carotid artery patency was improved (2 weeks after surgical ligation). …”
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Comparative transcriptomics in serial organs uncovers early and pan-organ developmental changes associated with organ-specific morphological adaptation by Marie Sémon, Marion Mouginot, Manon Peltier, Claudine Corneloup, Philippe Veber, Laurent Guéguen, Sophie Pantalacci

“…Abstract Mice have evolved a new dental plan with two additional cusps on the upper molar, while hamsters were retaining the ancestral plan. …”
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Endothelial Cell-Derived Extracellular Vesicles Target TLR4 via miRNA-326-3p to Regulate Skin Fibroblasts Senescence by Xinni Yang, Jiyong Tan, Jiqing Shen, Xin Zhang, Gaoxiang Huang, Xiaoxue Su, Jing Li

“…Furthermore, the skin tissues of aging mice were shown with overexpression of miR-326-3p and decrease of TLR4 gene and protein expression levels. …”
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Implantable Ultrasound‐Powered MXene/PVA Hydrogel‐Based Generator for Treatment of Glioblastoma by Xiaoping Hu, Ziyi Qiu, Yilin Yang, Ting Xu, Kai Sheng, Weicheng Lu, Jingdun Xie, Bingzhe Xu

“…In vitro and in vivo experiments confirmed the device's therapeutic effect, demonstrating a ≈92% inhibition rate in the growth of clinical tumor cells and a 73% reduction in tumor area in tumor‐bearing mice. The promising results indicate the broad application potential of the device in the treatment and prognostic improvement of GBM.…”
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Visualization of Small Vessels by Micro–Computed Tomography Using Titanium Dioxide Nanoparticles as a Novel Contrast Agent by Taku Goto, Ruriko Tanabe, Hirotoshi Shibuya, Masaru Tamura, Shintaro Nomura

“…Three-dimensionally reconstructed m-CT images in mice perfused with these contrast agents showed high resolution and accuracy in various organs without deformation or dilation of vessels. …”
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MEG3 Alleviated LPS-Induced Intestinal Injury in Sepsis by Modulating miR-129-5p and Surfactant Protein D by Xianjin Du, Dan Tian, Jie Wei, Chen Yan, Peng Hu, Xu Wu, Wenbin Yang

“…Then, MEG3 was overexpressed, and we found that its overexpression repressed the intestinal injury via downregulating miR-129-5p in sepsis mice. Moreover, TNF-α and IL-6 expression was elevated in intestinal tissues compared to the control groups. …”
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Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis by M. Isabel García-Laorden, Ingrid Stroo, Sanne Terpstra, Sandrine Florquin, Jan Paul Medema, Cornelis van´t Veer, Alex F. de Vos, Tom van der Poll

“…For this purpose, we used a murine model of E. coli intraperitoneal infection, resembling the clinical condition commonly associated with septic peritonitis by this bacterium, in wild-type and gzmA-deficient (gzmA−/−), gzmB−/−, and gzmAxB−/−mice. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm+ cells increased. …”
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Design, synthesis and evaluation of benzofuran-acetamide scaffold as potential anticonvulsant agent by Shakya Ashok K., Kamal Mehnaz, Balaramnavar Vishal M., Bardaweel Sanna K., Naik Rajashri R., Saxena Anil K., Siddiqui H. H.

“…The anticonvulsant activity of all synthesized compounds was assessed against the maximal electroshock induced seizures (MES) model in mice. Neurotoxicity was evaluated using the rotarod method. …”
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