A study on the differences in the gut microbiota and metabolism between male and female mice in different stress periods by Yajun Qiao, Yajun Qiao, Yajun Qiao, Juan Guo, Qi Xiao, Jianv Wang, Jianv Wang, Xingfang Zhang, Xingfang Zhang, Xinxin Liang, Xinxin Liang, Lixin Wei, Lixin Wei, Hongtao Bi, Tingting Gao, Tingting Gao

“…Microbial diversity analysis and metabolomics were performed on collected mouse feces. …”
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Identification of glutamine as a potential therapeutic target in dry eye disease by Xiaoniao Chen, Chuyue Zhang, Fei Peng, Lingling Wu, Deyi Zhuo, Liqiang Wang, Min Zhang, Zhaohui Li, Lei Tian, Ying Jie, Yifei Huang, Xinji Yang, Xiaoqi Li, Fengyang Lei, Yu Cheng

“…Our study demonstrated that co-treatment with mesenchymal stem cells (MSCs) and thymosin beta-4 (Tβ4) yielded the best therapeutic outcome against dry eye, surpassing monotherapy outcomes. In situ metabolomics through matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased glutamine levels in cornea following MSC + Tβ4 combined therapy. …”
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Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora by Qianqian Liu, Kexin Jiang, Shipeng Duan, Na Zhao, Yanmei Shen, Lirong Zhu, Ke-Qin Zhang, Jinkui Yang

“…Additionally, untargeted metabolomic analysis identified an important function of AoMsn2 in the modulation of secondary metabolites. …”
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Identification of serum metabolite biomarkers in premature infants with bronchopulmonary dysplasia: protocol for a multicentre prospective observational cohort study by Ping Zhou, Jun Chen, Ying Liu, Cheng Chen, Xin Guo, Zhangbin Yu, Jiebo Liu, Yanping Guo, Dan Dan Rao, Shengnan He, Xudong Yan, Wanxiang He, Songzhou Xu, Ruolin Zhang

“…These samples will undergo analysis using liquid chromatography-tandem mass spectrometry for untargeted metabolomics studies. Participants will be categorised into BPD and non-BPD groups based on their final diagnosis, and metabolite differences between these groups will be analysed. …”
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Baicalein induces apoptosis by inhibiting the glutamine-mTOR metabolic pathway in lung cancer by Jingyang Li, Di Zhang, Shaohui Wang, Peng Yu, Jiayi Sun, Yi Zhang, Xianli Meng, Juan Li, Li Xiang

“…In vitro experiments were used to assess the efficacy of baicalein (for H1299: 12.5, 25, and 50 μM; for A549: 10, 20, and 40 μM) on lung cancer cell proliferation, colony formation, and apoptosis. Metabolomics analysis was performed using liquid chromatography-mass spectrometry. …”
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Integrative computational analysis of anti-influenza potential in Caesalpinia mimosoides Lamk hydroethanolic extract by Anuwatchakij Klamrak, Shaikh Shahinur Rahman, Napapuch Nopkuesuk, Jaran Nabnueangsap, Jaraspim Narkpuk, Piyapon Janpan, Yutthakan Saengkun, Thananya Soonkum, Supawadee Sriburin, Samaporn Teeravechyan, Poramet Sitthiwong, Nisachon Jangpromma, Sirinan Kulchat, Kiattawee Choowongkomon, Rina Patramanon, Arunrat Chaveerach, Jureerut Daduang, Sakda Daduang

“…Through untargeted metabolomic analyses (UPLC-ESI(±)-QTOF-MS/MS) and subsequent stepwise computational metabolomics analyses, we identified the extract as primarily containing simple phenolics (e.g., gallic acid, ellagic acid, shikimic acid, and chlorogenic acid), flavonoid derivatives (e.g., quercetin, taxifolin, myricitrin, and afzelin), and other bioactive components, including dicarboxylic acids and germacrone. …”
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Lignin Metabolism Is Crucial in the Plant Responses to <i>Tambocerus elongatus</i> (Shen) in <i>Camellia sinensis</i> L. by Wenli Wang, Xiaogui Zhou, Qiang Hu, Qiuhong Wang, Yanjun Zhou, Jingbo Yu, Shibei Ge, Lan Zhang, Huawei Guo, Meijun Tang, Xin Li

“…Moreover, secondary metabolite analysis showed a significant increase in flavonoid compounds and lignin content after <i>T. elongatus</i> infestation. Metabolomics analysis of the flavonoid compounds revealed a decrease in the proanthocyanidin level and an increase in anthocyanidin glycosides (anthocyanins and their derivatives) after <i>T. elongatus</i> infestation. …”
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A novel α-conotoxin [D1G, ΔQ14] LvIC decreased mouse locomotor activity by Wen Wang, Wen Wang, Meiting Wang, Huanbai Wang, Weifeng Xu, Conggang Wang, Jie Pei, Xiaodan Li, Dongting Zhangsun, Dongting Zhangsun

“…To investigate the molecular alterations in the mice’s brains, untargeted metabolomics and label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) were employed. …”
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Folic acid alleviates the negative effects of dexamethasone induced stress on production performance in Hyline Brown laying hens by Xi Sun, Chaohui Wang, Sijing Li, Xiaoying Liu, Yun Li, Yumeng Wang, Yuxin Niu, Zhouzheng Ren, Xin Yang, Xiaojun Yang, Yanli Liu

“…Kyoto Encyclopedia of Genes and Genomes enrichment from the transcriptome and metabolomics showed that the reduced production performance may be due to the disturbance of oocyte production, calcium metabolism, and oxidative stress. …”
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Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by Bacteroides and their extracellular vesicles by Basit Yousuf, Walid Mottawea, Galal Ali Esmail, Nazila Nazemof, Nour Elhouda Bouhlel, Emmanuel Njoku, Yingxi Li, Xu Zhang, Zoran Minic, Riadh Hammami

“…Using untargeted and targeted metabolomics, we identified key neurotransmitter-related or precursor metabolites, including GABA, l-tryptophan, 5-HTP, normelatonin, kynurenic acid, l-tyrosine, and norepinephrine, in a strain- and media-specific manner, with GABA (1–2 mM) being the most abundant. …”
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Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling by Walid Mottawea, Basit Yousuf, Salma Sultan, Tamer Ahmed, JuDong Yeo, Nico Hüttmann, Yingxi Li, Nour Elhouda Bouhlel, Hebatoallah Hassan, Xu Zhang, Zoran Minic, Riadh Hammami

“…First, we conducted a multi-omics profiling of MEVs’ contents from ex vivo and stool samples. Metabolomics analysis identified various neuro-related compounds encapsulated within MEVs, such as arachidonyl-dopamine, gabapentin, glutamate, and N-acylethanolamines. …”
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Sleep Promotion by 3-Hydroxy-4-Iminobutyric Acid in Walnut Diaphragma juglandis Fructus by Jian Ji, Yongli Ye, Lina Sheng, Jiadi Sun, Qianqian Hong, Chang Liu, Jun Ding, Shuxiang Geng, Deping Xu, Yinzhi Zhang, Xiulan Sun

“…In this investigation, we analyzed the sedative and hypnotic components of the walnut Diaphragma juglandis fructus and innovatively discovered a compound, defined as 3-hydroxy-4-iminobutyric acid (HIBA), which disrupts motor activity and enhances sleep duration by regulating the neurotransmitters (GABA, DA, etc.) within the brain and serum of mice. Subsequently, a metabolomics approach of the serum, basal ganglia, hypothalamus, and hippocampus as well as the gut microbiota was undertaken to unravel the underlying molecular mechanisms of sleep promotion. …”
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Medium- to long-term health condition of patients post-COVID-19, exercise intolerance and potential mechanisms: A narrative review and perspective by Fabian Schwendinger, Denis Infanger, Debbie J Maurer, Thomas Radtke, Justin Carrard, Julia M Kröpfl, Aglaia Emmenegger, Henner Hanssen, Christoph Hauser, Udo Schwehr, Hans H Hirsch, Julijana Ivanisevic, Karoline Leuzinger, Aurélien E Martinez, Marc Maurer, Thomas Sigrist, Lukas Streese, Roland von Känel, Timo Hinrichs, Arno Schmidt-Trucksäss

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Characteristics and multi-omics analysis of spontaneous spondyloarthritis in non-human primates: Case report by Lei Cai, Qing Lv, Ronghua Ma, Wei Liu, Yalun Guan, Zhongqiang Huang, Qingyu Liu, Yunfeng Li, Shuhua Liu, Ge Li, Yu Zhang

“…To delve into the pathological and physiological traits of spontaneous non-human primate spondyloarthritis, this study meticulously examined the disease features of this natural disease model through an array of techniques including X-ray imaging, MRI imaging, blood biochemistry, markers of bone metabolism, transcriptomics, proteomics, and metabolomics. X-ray imaging results revealed that crab-eating monkeys (Macaca fascicularis) with spontaneous spondyloarthritis developed bone spurs in the spine and experienced bone destruction in peripheral joints. …”
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Dihydroquercetin attenuated Prevotella copri-caused intestinal injury by modulating gut microbiota and bile acids in weaned piglets by Long Wang, Ruizhi Hu, Siqi Ma, Xizi Yang, Jiatai Gong, Hongkun Xiang, Mingkun Shi, Xupeng Yuan, Liang Chen, Hongfu Zhang, Bie Tan, Xi He, Jianhua He, Shusong Wu

“…Intestinal flora analysis showed that P. copri increased the relative abundance of Prevotella (P = 0.026) and Eubacterium coprostanoligenes group (P < 0.001), but decreased the relative abundance of Lachnospiraceae NK4A136 group (P < 0.001), while supplementation of DHQ reduced the relative abundance of Prevotella (P = 0.026). Metabolomics results indicated that P. copri enhanced the content of 12-OH bile acid, but decreased the contents of glycodeoxycholic acid (GDCA) and glycochenodeoxycholic acid (GCDCA) (P < 0.001), while DHQ reduced the 12-OH bile acid content (P < 0.001) and increased the GDCA content (P = 0.020). …”
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Skatole Alleviates Osteoarthritis by Reprogramming Macrophage Polarization and Protecting Chondrocytes by Weiyun Wang, Yaru Chu, Yunkun Lu, Jie Xu, Weixuan Zhao, Zhuo Liang, Xueqiang Guo, Lingling Xi, Tao Han, Yaping Shen, Wenjuan Song, Yanhua Tang, Mengnan Wen, Zhuang Qian, Lei Wang, Zhenlin Fan, Guangdong Zhou, Wenjie Ren

“…RNA-sequencing analysis, targeted metabolomics, and mitochondrial stress tests further revealed that skatole treatment shifted macrophages toward oxidative phosphorylation for energy production. …”
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Bifidobacterium animalis ssp. lactis BX-245-fermented milk alleviates tumor burden in mice with colorectal cancer by Chengcong Yang, Musu Zha, Lu Li, Jiaqi Qiao, Lai-Yu Kwok, Dandan Wang, Yongfu Chen

“…The study monitored changes in tumor size and number, gut microbiota, metabolomics, and inflammation levels before and after the intervention. …”
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Invited review: Improved control of Johne's disease in dairy cattle through advancements in diagnostics, testing, and management of young stock by Larissa Martins, Karin Orsel, Razieh Eshraghisamani, Jose Miguel Hernández-Agudelo, A. Caroline Pereira, Waseem Shaukat, Ad P. Koets, John P. Bannantine, Caroline Ritter, David F. Kelton, Richard J. Whittington, Maarten F. Weber, Antonio Facciuolo, Navneet K. Dhand, Karsten Donat, Susanne Eisenberg, Miguel A. Salgado, John P. Kastelic, Jeroen De Buck, Herman W. Barkema

“…New diagnostics have been developed, including phage-based tests to detect viable MAP, as well as gene expression patterns and metabolomics to detect MAP-infected young stock. In addition, refinements in testing and management of calves and heifers may enable reductions in MAP prevalence. …”
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Attentional dysfunction arises from right frontocentral and occipital network connectivity in Parkinson's disease by Isobel Timothea French, Kuo-Hsuan Chang, Wei-Kuang Liang, Wen-Sheng Chang, Yen-Shi Lo, Yi-Ru Wang, Mei-Ling Cheng, Norden E. Huang, Hsiu-Chuan Wu, Siew-Na Lim, Chiung-Mei Chen, Chi-Hung Juan

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Effect of the gut core microbiota Cetobacterium on the growth, physiology, and nutritional metabolism of Nile tilapia (Oreochromis niloticus) by Miao Wang, Chenglin Tang, Ziyue Zhang, Zijian Fan, Lijin Jiang, Zhigang Liu, Jianmeng Cao, Zhang Wang, Maixin Lu, Mengmeng Yi, Xiaoli Ke

“…Additionally, we examined the transcriptome of the liver and the metabolome of the serum in tilapia. Our results showed that dietary supplementation with NK01 significantly improved carcass ratio, increased levels of insulin and amino acids in the serum, and reduced serum glucose and TG levels as well as gut carbohydrate content. …”
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