Antioxidant and Antimicrobial Properties of Cactus Pear (Opuntia) Seed Oils by Esther Ramírez-Moreno, Raquel Cariño-Cortés, Nelly del Socorro Cruz-Cansino, Luis Delgado-Olivares, José Alberto Ariza-Ortega, Vanessa Yelina Montañez-Izquierdo, María Manuela Hernández-Herrero, Tomás Filardo-Kerstupp

“…The fatty acid profile was analyzed by gas chromatography-mass spectrometry (GC-MS). Oil from green cactus pear seeds obtained with ethanol and ethyl acetate exhibited higher antioxidant activity (p<0.05) of 323 and 316 μmol TE/20 mg (p < 0.05), respectively, compared to red cactus pear seed oil (≈274 and 247 μmol TE/20 mg with ethyl acetate and ethanol, resp.). …”
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Quantifying LAGA mutated mouse IgG2a monoclonal antibody with a rapid pepsin digestion enabled immunoaffinity LC/MS/MS assay by Linlin Dong, Susan Chen, Konstantin Piatkov, Dong Wei, Mark G. Qian

“…Consequently, a new pepsin digestion-based liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was successfully developed to support the mouse pharmacokinetic (PK) sample analysis. …”
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Seasonal investigation of ultrafine-particle organic composition in an eastern Amazonian rainforest by A. E. Thomas, H. S. Glicker, A. B. Guenther, R. Seco, O. Vega Bustillos, J. Tota, R. A. F. Souza, J. N. Smith

“…Samples collected during the three time periods were compiled and analyzed using liquid chromatography coupled with Orbitrap high-resolution mass spectrometry. Our findings suggest a sustained influence of isoprene organosulfate chemistry on ultrafine particles from the different periods. …”
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Enhancing the targeted and untargeted analysis of honey by vacuum-assisted SPME-GC × GC-MS. A green, practical, and highly informative approach by Damien Eggermont, Francesca Pardi, Giorgia Purcaro

“…In this work, we explored the use of Vac-HS coupled with comprehensive two-dimensional gas chromatography coupled with mass spectrometry to simultaneously determine 5-hydroxymethylfurfural (a semi-volatile processing marker in honey) and the entire volatile profile, which is recognized as a highly informative fingerprint of the geographical and botanical authenticity of honey. …”
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Evaluation of the Inhibitory Effect of Moringa oleifera Leaves Methanolic Extract against In Vitro Growth of Several Babesia Species and Theileria equi and the In Vivo Growth of Ba... by Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mohamed Z. Sayed-Ahmed, Yosif Almoshari, Saad S. Alqahtani, Sarfaraz Ahmad, Nawazish Alam, Basma H. Marghani, Abdelbaset E. Abdelbaset, Ikuo Igarashi

“…Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL’s anti-piroplasm efficacy was assessed both in vitro and in vivo using the SYBR Green I fluorescence assay. …”
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LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation by Litao Liang, Wenbo Jia, Jinyi Wang, Yanzhi Feng, Deming Zhu, Wenhu Zhao, Chao Xu, Xiangyu Ling, Qingpeng Lv, Xiaoming Ai, Lianbao Kong, Wenzhou Ding

“…Nile red staining and lipid assays showed that LRP11 knockdown significantly reduces lipid accumulation in HCC cells, with a concurrent decrease in key lipid metabolism markers such as FSAN, ACLY and ACSL4, as demonstrated by Western blotting. Mass spectrometry (MS) and co-immunoprecipitation (Co-IP) revealed that LRP11 recruits USP5, enhancing USP5-mediated deubiquitination of RACK1. …”
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In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants by Roggers, Gang, Richard, Komakech, Yuseong, Chung, Denis, Okello, Wook, Jin Kim, Byeong, Cheol Moon, Nam, Hui Yim, Youngmin, Kang

“…Furthermore, the tissues (roots and shoots) of maternal and in vitro‑regenerated C. pilosula plants were subjected to Fourier‑transform near‑infrared (FT‑NIR) spectrometry, Gas chromatography‑mass spectrometry (GC–MS), and their total flavonoids, phenolics, and antioxidant capacity were determined and compared. …”
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The antibacterial and antifungal potential of marine natural ingredients from the symbiont bacteria of mangrove by D. Pringgenies, W. Ari Setyati, F. Feliatra, D. Ariyanto

“…Further, everal tests were conducted, including the antibacterial test, antifungal test, molecular identification, and gas chromatography-mass spectrometry. The pathogenic bacteria used in the antibacterial test were Staphylococcus aureus, Vibrio harveyi, and Vibrio alginolyticusFINDINGS: The antibacterial test results showed that eight isolates were capable of producing an inhibition zone against S. aureus, seven isolates were positive for antibacterial activity against Vibrio harveyi, and 10 isolates were positive for antibacterial activity against Vibrio alginolyticus. …”
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Proteomic profiling of serum in cats with naturally occurring degenerative joint disease and co-morbid conditions by B. Duncan X. Lascelles, B. Duncan X. Lascelles, B. Duncan X. Lascelles, B. Duncan X. Lascelles, Rakesh Ponnala, Steven G. Kamerling, Tracey Williams

“…A greater understanding of the systems biology of DJD, DJD-associated pain, and CKD may contribute to identifying disease specific biomarkers and relevant targets for the development of therapeutics for the control of these conditions in cats, and help inform human pain therapeutic development.MethodsUsing mass spectrometry-based proteomic profiling of the serum of 200 highly phenotyped cats with varying burdens of DJD, pain, and CKD, we identified significant individual proteins and pathways.ResultsFunctional pathway analysis, based on differentially abundant proteins across individual disease states (DJD, pain, CKD), identified pathways playing a role in DJD and DJD-associated pain including acute phase response signaling, LXR/RXR and FXR/RXR activation and the complement system. …”
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Purification, Characterization and Antibacterial Mechanism of Plantaricin 2-1 by Mengyang LI, Xiaoxue CHE, Qingshen SUN

“…To explore the basic properties and antibacterial mechanism of bacteriocin produced by Lactobacillus plantarum L3, the strain was sub-cultured in MRS medium, and the broth was extracted by ethyl acetate extraction, followed by purification serially through dextran gel chromatography, cation exchange chromatography and reverse phase high performance liquid chromatography (RP-HPLC), and finally identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Listeria monocytogenes and Escherichia coli were used as indicator strain and the antibacterial mechanism of the plantaricin was determined by crystal violet staining, scanning electron microscopy, infrared spectroscopy, fluorescence spectroscopy, extracellular alkaline phosphatase (AKP), ATP, and lactate dehydrogenase (LDH) content analysis. …”
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Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis by Yun-Long Guo, Yang Wang, Yi-Lin Zhao, Xiu-Ying Xu, Hao Zhang, Cheng-Bin Zhao, Ming-Zhu Zheng, Shu-Ying Liu, Yu-Zhu Wu, Jing-Sheng Liu

“…Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). …”
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Extraction of Saponins from Xanthoceras sorbifolium Bunge Leaves Using Deep Eutectic Solvents and Their Inhibitory Activity against Postharvest Pathogenic Fungi by Xue LI, Xinyu CHU, Erzheng SU, Jiahong WANG

“…The extracts were characterized using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The mechanism of XLs saponins extraction by DES was investigated by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). …”
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Antiviral Activity of the Marine Haptophyta <i>Diacronema lutheri</i> by Eleonora Montuori, Annalisa Ambrosino, Gerardo Della Sala, Costanza Ragozzino, Gianluigi Franci, Carla Zannella, Anna De Filippis, Donatella de Pascale, Massimiliano Galdiero, Chiara Lauritano

“…Liquid chromatography coupled with untargeted high-resolution tandem mass spectrometry (LC-HRMS²) were employed to chart the metabolite distribution in all SPE fractions and pinpoint molecular families unique (or almost unique) to the bioactive fraction. …”
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Comparison of Extracellular Metabolites and Antioxidant Activity of Different Strains of Wolfiporia hoelen (Fr.) Y.C. Dai &amp;V. Papp by TAN Yun, SHEN Airong, SHEN Baoming, YU Jinxiu, LIU Lina, LI Sainan, TAN Zhuming

“…Totally 55 flavonoids and 33 phenolic compounds were qualitatively detected using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS). The relative content of each component varied among different W. hoelen strains; the most abundant flavonoid was malonyldaidzin and the most abundant phenolic component was 3-formylsalicylic acid in W. hoelen strains FLA, FGD, FGZ, FHB, GTR1, XJ28 and F5.78. …”
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Corrosion inhibition effects of eco-friendly clarithromycin molecules on aluminium in hydrochloric acid solution via experimental, theoretical and optimization approach by O.D. Onukwuli, I.A. Nnanwube, F.O. Ochili, M. Omotioma, J.I. Obibuenyi

“…Characterization of the inhibitor was executed with gas chromatography mass spectrometry (GC–MS) and Fourier transform infrared (FTIR) spectroscopy, while optimization of the process was executed with response surface methodology (RSM). …”
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Recovery of Phenolic Compounds with Antioxidant Capacity Through Solid-State Fermentation of Pistachio Green Hull by Andrés Javier Ordoñez-Cano, Ulises Ramírez-Esparza, Fernando Méndez-González, Mónica Alvarado-González, Ramiro Baeza-Jiménez, Leonardo Sepúlveda-Torre, Lilia Arely Prado-Barragán, José Juan Buenrostro-Figueroa

“…Gallic acid 4-<i>O</i>-glucoside and geranine were identified in the PGH extracts using high-performance liquid chromatography coupled with mass spectrometry. The SSF provides eco-friendly alternatives for releasing bioactive compounds from PGH, adding value to this waste.…”
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In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergi... by Laila Muñoz Castellanos, Nubia Amaya Olivas, Juan Ayala-Soto, Carmen Miriam De La O Contreras, Miriam Zermeño Ortega, Fabiola Sandoval Salas, Leon Hernández-Ochoa

“…Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.…”
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Pharmacokinetics of Eight Flavonoids in Rats Assayed by UPLC-MS/MS after Oral Administration of Drynariae rhizoma Extract by Zhan-Ling Xu, Ming-Yue Xu, Hai-Tao Wang, Qing-Xuan Xu, Ming-Yang Liu, Chun-Peng Jia, Fang Geng, Ning Zhang

“…In order to make good and rational use of Drynariae rhizoma in future, a rapid, sensitive, and selective ultraperformance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed to investigate the pharmacokinetics of eight main flavonoids in rat plasma after oral administration of the Drynariae rhizoma extract, including neoeriocitrin, luteolin-7-O-β-D-glucoside, astragalin, naringin, eriodictyol, luteolin, naringenin, and kaempferol. …”
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Hair Mercury Levels Detection in Fishermen from Sicily (Italy) by ICP-MS Method after Microwave-Assisted Digestion by Giuseppe Giangrosso, Gaetano Cammilleri, Andrea Macaluso, Antonio Vella, Nicolantonio D’Orazio, Stefania Graci, Gianluigi Maria Lo Dico, Fabio Galvano, Margherita Giangrosso, Vincenzo Ferrantelli

“…A number of ninety-six hair samples from Sicilian fishermen were examined for total mercury detection by an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) method. The mercury levels obtained were compared with mercury levels of 96 hair samples from a control group, in order to assess potential exposure to heavy metals of Sicilian fishermen due to fish consumption and closeness to industrial activities. …”
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Precision fetal cardiology detects cyanotic congenital heart disease using maternal saliva metabolome and artificial intelligence by Ray Bahado-Singh, Nadia Ashrafi, Amin Ibrahim, Buket Aydas, Ali Yilmaz, Perry Friedman, Stewart F. Graham, Onur Turkoglu

“…Metabolomic analyses using Ultra-High Performance Liquid Chromatography/Mass Spectrometry identified 468 metabolites in the saliva. …”
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