Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors by Yuliang Liu, Michael S. Lee, Mark A. Olson, Ronald N. Harty

“…To address this gap, we used a bimolecular complementation (BiMC) approach to detect, localize, and follow the trafficking patterns of eVP40-Tsg101 complexes in live mammalian cells. In addition, we used the BiMC approach along with a VLP budding assay to test small molecule inhibitors identified by in silico screening for their ability to block eVP40 PTAP-mediated interactions with Tsg101 and subsequent budding of eVP40 VLPs. …”
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Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells by Vanessa R. Bevilaqua, Gabriel F. Pelentir, Moema A. Hausen, Eliana A. R. Duek, Vadim R. Viviani

“…However, it is known that mammalian cells require temperatures higher than 36 °C, which red-shift the bioluminescence spectra of most firefly luciferases, decreasing their activities and the resolution of ratiometric pH analysis. …”
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Unraveling the function of TSC1-TSC2 complex: implications for stem cell fate by Shuang Wang, Ruishuang Ma, Chong Gao, Yu-Nong Tian, Rong-Gui Hu, Han Zhang, Lan Li, Yue Li

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RNA modifications: emerging players in the regulation of reproduction and development by Wen Junfei, Zhu Qifan, Liu Yong, Gou Lan-Tao

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NOP2/Sun RNA Methyltransferase 4 Regulates the Mammalian Target of Rapamycin Signaling Pathway to Promote Hepatocellular Carcinoma Progression by Congren Wang, Shaoying Ke, Shaoze Lin, Conglin Lin, Zhibing Cai, Lingju Hong, Qunxiong Pan

“…The western blot was utilized to determine the protein expression level of NSUN4 and mammalian target of rapamycin (mTOR) pathway-related proteins in cells and mouse tumor tissues. …”
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Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells by Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch

“…The SsdA-base editor efficiently converts C-to-T in rice and barley protoplasts and induces mutations in rice plants and mammalian cells. The engineered SsdA is a highly efficient genome editing tool.…”
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Isoleucine at position 137 of haemagglutinin acts as a mammalian adaptation marker of H9N2 avian influenza virus by Weiwei Ma, Chenyang Ren, Lin Shi, Bo Meng, Yali Feng, Ying Zhang

“…Our findings demonstrated that HA-137I is a key molecular marker for mammalian adaptation of H9N2 AIV.…”
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Computational model for mammalian circadian oscillator: interacting with NAD+/SIRT1 pathway and age-related changes in gene expression of circadian oscillator by N. L. Podkolodnyy, N. N. Tverdokhleb, O. A. Podkolodnaya

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Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells by Yuanxiu Zhong, Yiyun Duan, Fenju Lai, Jinhua Zhang, Yimin Dai

“…In this study, we identify that LI0758 can be secreted by the Yersinia T3SS, which suppresses yeast growth and activates mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathway in mammalian cells. Bioinformatics analyses indicate that LI0758 is an ortholog of Rce1, a eukaryotic CAAX protein endoprotease, sharing a similar subcellular localization on the endoplasmic reticulum (ER). …”
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Development of a Reference Method and Materials for Quantitative Measurement of UV-Induced DNA Damage in Mammalian Cells: Comparison of Comet Assay and Cell Viability by Donald H. Atha, Alessandro Tona, Vytas Reipa

“…Reference methods and materials for quantitative measurement of UVA-induced DNA damage in mammalian cells are frequently needed. In this study, we examined the use of the single-cell gel electrophoresis (comet) assay to assess the UVA-induced DNA damage in surface-attached Chinese hamster ovary (CHO) cells treated with a photosensitizer as a candidate cellular oxidative damage reference material. …”
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A protocol for loading Calcein-AM into extracellular vesicles from mammalian cells for clear visualization with a fluorescence microscope coupled to a deconvolution system. by María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero

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Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids by Xiuling Cao, Xiang Wu, Lei Zhao, Ju Zheng, Xuejiao Jin, Xinxin Hao, Joris Winderickx, Shenkui Liu, Lihua Chen, Beidong Liu

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Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line by Mahshid Mowla, Gilar Gorji-Bahri, Hamid Reza Moghimi, Atieh Hashemi

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Left ventricular hypertrophy in young hypertensives: the possible crosstalk of mTOR and angiotensin-II -a case-control study by Busayo Onafowoke Oguntola, Stephen Olawale Oguntola, Opeyemi Ezekiel Ojo, Pauleen Ayomide Ukpabio, Adams Olalekan Omoaghe, Kehinde Samuel Olaniyi

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PB2 and PA mutations contribute to the pathogenicity of mouse-adapted pdmH1N1-Venus reporter influenza A virus in a mammalian model by Shixiang Wu, Ruonan Yi, Yingying Tao, Huimin Wu, Li Wu, Jiasheng Song, Xin Zhang, Beibei Yang, Xing Wu, Yulong He, Jianhong Shu, Huapeng Feng

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Association of Bone Loss with the Upregulation of Survival-Related Genes and Concomitant Downregulation of Mammalian Target of Rapamycin and Osteoblast Differentiation-Related Genes in the Peripheral Blood of Late Postmenopausal Osteoporotic Women by Elena V. Tchetina, Karina A. Maslova, Mikhail Y. Krylov, Valery A. Myakotkin

“…OP registered by low BMD indices in late postmenopausal patients was associated with a significant upregulation of autophagy protein ULK1, cyclin-dependent kinase inhibitor p21, and metalloproteinase MMP-9 gene expression in the blood compared to the healthy controls and in a significant downregulation of mTOR (mammalian target of rapamycin), RUNX2, and ALPL gene expression, while expression of cathepsin K, caspase-3, transforming growth factor (TGF)β1, interleukin- (IL-) 1β, and tumor necrosis factor α (TNFα) was not significantly affected. …”
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RESULTS OF CLINICAL TRIALS OF MEDICAL TREA TMENT OF RENAL CELL CARCINOMA IN 2014–2015 by A. M. Popov, O. B. Karyakin, A. D. Kaprin

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Phagocytosis by macrophages decreases the radiance of bioluminescent Staphylococcus aureus by Elles C. Boonstra, Liliana Agresti, Henny C. van der Mei, Paul C. Jutte, Jelmer Sjollema

Subjects: “…Coculture of bacteria and mammalian cells…”
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Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines by Vytas Reipa, Vincent A. Hackley, Alessandro Tona, Min Beom Heo, Ye Ryeong Lee, Tae Geol Lee, Aaron Johnston-Peck, Tae Joon Cho

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Molecular mechanisms of autism as a form of synaptic dysfunction by E. A. Trifonova, T. M. Khlebodarova, N. E. Gruntenko

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