MCP5, a methyl-accepting chemotaxis protein regulated by both the Hk1-Rrp1 and Rrp2-RpoN-RpoS pathways, is required for the immune evasion of Borrelia burgdorferi. by Sajith Raghunandanan, Kai Zhang, Yan Zhang, Raj Priya, Ching Wooen Sze, Yongliang Lou, Michael J Lynch, Brian R Crane, Mark H Kaplan, Chunhao Li, X Frank Yang

“…These findings reveal that MCP5, regulated by both the Rrp1 and Rrp2 pathways, is critical for establishing infection in mammalian hosts by evading NK cell-mediated host innate immunity and is important for the transmission of spirochetes from ticks to mammalian hosts. …”
Get full text

Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude by Anne Neuss, Thomas Steimann, Jacinta Sofia Tomas Borges, Robert Dinger, Jørgen Barsett Magnus

“…Abstract Background For process development in mammalian cell cultivations, scale-up approaches are essential. …”
Get full text

Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells by Zixi Yin, Lingyi Chen

“…However, the diploid status of most mammalian cells restricts the application of CRISPR/Cas9 in genetic screening. …”
Get full text

The Controversy, Challenges, and Potential Benefits of Putative Female Germline Stem Cells Research in Mammals by Zezheng Pan, Mengli Sun, Xia Liang, Jia Li, Fangyue Zhou, Zhisheng Zhong, Yuehui Zheng

“…However, in recent years, researchers have successfully isolated and cultured a type of germ cell from postnatal ovaries in a variety of mammalian species that have the abilities of self-proliferation and differentiation into oocytes, and this finding indicates that putative germline stem cells maybe exist in the postnatal mammalian ovaries. …”
Get full text

Myosin-based nucleation of actin filaments contributes to stereocilia development critical for hearing by Zane G. Moreland, Fangfang Jiang, Carlos Aguilar, Melanie Barzik, Rui Gong, Ghazaleh Behnammanesh, Jinho Park, Arik Shams, Christian Faaborg-Andersen, Jesse C. Werth, Randall Harley, Daniel C. Sutton, James B. Heidings, Stacey M. Cole, Andrew Parker, Susan Morse, Elizabeth Wilson, Yasuharu Takagi, James R. Sellers, Steve D. M. Brown, Thomas B. Friedman, Gregory M. Alushin, Michael R. Bowl, Jonathan E. Bird

Get full text

Generation of stably expressing IRF5 spliced isoform in Jurkat cells by Ashwinder Kaur, Chee-Mun Fang

“… Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein function. …”
Get full text

Abundance, Species Diversity, and Distribution of Diurnal Mammals in Humbo Community-Based Forest Area, Southern Ethiopia by Adanech Lemma, Wondimagegnehu Tekalign

“…The highest number of mammalian species were distributed in bushland habitat during both wet (n = 7) and dry (n = 7) seasons. …”
Get full text

Role of the Endocannabinoid System in the Central Regulation of Nonmammalian Vertebrate Reproduction by Erika Cottone, Valentina Pomatto, Patrizia Bovolin

“…The endocannabinoid system (ECS) has a well-documented pivotal role in the control of mammalian reproductive functions, by acting at multiple levels, that is, central (CNS) and local (gonads) levels. …”
Get full text

The Regulation and Functions of Endogenous Retrovirus in Embryo Development and Stem Cell Differentiation by Yangquan Xiang, Hongqing Liang

“…During the course of life, ERVs are associated with multiple aspects of chromatin and transcriptional regulation in development and pathological conditions. In mammalian embryos, ERVs are extensively activated in early embryo development, but with a highly restricted spatial-temporal pattern; and they are drastically silenced during differentiation with exceptions in extraembryonic tissue and germlines. …”
Get full text

Sirtuin activators as an anti-aging intervention for longevity by Puja Sah, Anita K. Rai, Donkupar Syiem

“…This review discusses mammalian sirtuins in aging, the small molecules that activate sirtuins, modulation of sirtuin activity, and its impact in alleviating the effects of aging.…”
Get full text

Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery by Christine N. Vuong, Kevin M. Reynolds, Gerry S. Rivera, Baisen Zeng, Zahra Karimpourkalou, Manith Norng, Yulei Zhang, Robayet Chowdhury, Darlene Pedersen, Melissa Pantoja, Ellen Collarini, Swetha Garimalla, Shelley Izquierdo, Eric G. Vajda, Brett Antonio, Devendra B. Srivastava, Marie-Cecile van de Lavoir, Yasmina Abdiche, William Harriman, Philip A. Leighton

“…Domain antibodies have commonly been sourced from camelids with ex-vivo humanization or transgenic rodents expressing heavy chains without light chains, but these host species are all mammalian, limiting their capacity to elicit robust immune responses to conserved mammalian targets. …”
Get full text

Evolution and Development of Dual Ingestion Systems in Mammals: Notes on a New Thesis and Its Clinical Implications by Jeffrey R. Alberts, Rita H. Pickler

“…First, we apply an evolutionary perspective, recognizing that suckling-feeding is a universal, mammalian developmental sequence. We find that in mammalian evolution, feeding systems in offspring were established prior to the evolution of lactation, and therefore suckling is a separable feature that was added to feeding. …”
Get full text

Ensuring the Stability of the Genome: DNA Damage Checkpoints by Christine Latif, Susan H. Harvey, Susan J. O'Connell

“…This is reflected by the functional conservation of these pathways in all eukaryotes from yeast to mammalian cells. This review will examine the cellular response to DNA damage throughout the cell cycle. …”
Get full text

A Toxicological Evaluation of Methylliberine (Dynamine®) by Timothy S. Murbach, Róbert Glávits, John R. Endres, Amy E. Clewell, Gábor Hirka, Adél Vértesi, Erzsébet Béres, Ilona Pasics Szakonyiné

“…A (1) bacterial reverse mutation test, (2) in vitro mammalian chromosomal aberration test, (3) in vivo mammalian micronucleus test, and (4) 90-day repeated-dose oral toxicity study in rats with a 28-day recovery period were conducted. …”
Get full text

Widespread release of translational repression across Plasmodium's host-to-vector transmission event. by Kelly T Rios, James P McGee, Aswathy Sebastian, Sanjaya Aththawala Gedara, Robert L Moritz, Marina Feric, Sabrina Absalon, Kristian E Swearingen, Scott E Lindner

“…Malaria parasites must respond quickly to environmental changes, including during their transmission between mammalian and mosquito hosts. Therefore, female gametocytes proactively produce and translationally repress mRNAs that encode essential proteins that the zygote requires to establish a new infection. …”
Get full text

Stem Cell Interaction with Somatic Niche May Hold the Key to Fertility Restoration in Cancer Patients by Deepa Bhartiya, Kalpana Sriraman, Seema Parte

“…Postnatal oogenesis in mammalian ovary from VSELs may be exploited for fertility restoration in cancer survivors including those who were earlier deprived of gametes and/or gonadal tissue cryopreservation options.…”
Get full text

Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line by Khaled Y. Abdel-Halim, Safaa R. Osman

Get full text

Traffic Control of Bacteria-Derived Molecules: A New System of Host-Bacterial Crosstalk by Hiroaki Konishi, Mikihiro Fujiya, Yutaka Kohgo

“…Virulent microorganisms, such as pathogenic bacteria and viruses, are recognized by pattern recognition receptors (PRRs), including toll-like receptors (TLRs) and nucleotide-binding oligomerization-domain proteins (NODs), and induce inflammatory responses in mammalian hosts. Conversely, commensal bacteria and probiotics, which symbiotically confer health benefits on the host organisms, can lodge in the host intestinal tract without inducing intestinal inflammation. …”
Get full text

Single-cell chromatin accessibility landscape profiling reveals the diversity of epigenetic regulation in the rat nervous system by Peiyao Ma, Shanshan Duan, Wen Ma, Qiuting Deng, Yeya Yu, Peng Gao, Yue Yuan, Chuanyu Liu

“…Abstract The mammalian nervous system controls complex functions through highly specialized and interacting structures. …”
Get full text

Production of Toxic Volatile Trimethylbismuth by the Intestinal Microbiota of Mice by Britta Huber, Philip Dammann, Christine Krüger, Petra Kirsch, Beatrix Bialek, Roland A. Diaz-Bone, Reinhard Hensel

“…The biotransformation of metals and metalloids into their volatile methylated derivatives by microbes growing under anaerobic conditions (e.g., the mammalian intestinal microbiota) plays an important role in spreading these compounds in the environment. …”
Get full text