CD4 expression controls epidermal stem cell balance by Nadine Brandes, Heidi Hahn, Anja Uhmann

“…Abstract The balance of stem cell populations is essential for the maintenance, renewal, and repair of the mammalian epidermis. Here, we report that CD4, which is a typical marker of helper T cells, monocytes, macrophages, and dendritic cells, is also expressed on murine K5+ keratinocytes. …”
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Fluo-Cast-Bright: a deep learning pipeline for the non-invasive prediction of chromatin structure and developmental potential in live oocytes by Xiangyu Zhang, Claudia Baumann, Rabindranath De La Fuente

“…Abstract In mammalian oocytes, large-scale chromatin organization regulates transcription, nuclear architecture, and maintenance of chromosome stability in preparation for meiosis onset. …”
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CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers by Qiwen Su-Tobon, Jiayi Fan, Michael Goldstein, Kevin Feeney, Hongyuan Ren, Patrick Autissier, Peiyi Wang, Yingzi Huang, Udayan Mohanty, Jia Niu

“…Application of orthogonal aptamer-RBP pairs in multiplexed CRISPR allows effective simultaneous transcriptional activation and repression of endogenous genes in mammalian cells.…”
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IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice by Tingwenyi Hu, Xiangrong Tang, Tiechao Ruan, Shunhua Long, Guicen Liu, Jing Ma, Xueqi Li, Ruoxuan Zhang, Guoning Huang, Ying Shen, Tingting Lin

“…Protein mass spectrometry, western blotting, and bioinformatic analyses were performed to identify critical components of mammalian RS1 to model its structure and explore the pathological mechanism of IQUB deficiency. …”
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Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles by Hanjing Li, Yining Liu, Yuqing Lin, Sijia Li, Chenlu Liu, Ao Cai, Wei Li, Wanyu Zhang, Xinlu Gao, Zhongyu Ren, Haoyu Ji, Yang Yu, Xiuxiu Wang, Wenya Ma, Ning Wang, Dan Zhao, Tianlong Li, Yu Liu, Benzhi Cai

“…Abstract Neonatal mammalian hearts are capable of regenerating by inducing cardiomyocyte proliferation after injury. …”
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Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter by Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen

“…We describe steps for designing guide RNA to direct Cas9 endonuclease to consensus splice sites, producing transgenic mice through pronuclear injection, and screening for desired mutations in cultured mammalian cells using a minigene splicing reporter. …”
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A Heating and Cooling Stage With Fast Temporal Control for Biological Applications by Manon Valet, Juan M. Iglesias-Artola, Falk Elsner, Anatol W. Fritsch, Otger Campas

“…We envision that this device could benefit the communities using model and non-model organisms with physiological temperatures different from typical mammalian cell culture incubation in biomedical research.…”
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Response of host immune system to tick borne encephalitis virus by Iveta Stibraniova, Pavlina Bartikova, Jozef Dzubara

“…Tick-borne encephalitis virus (TBEV, Flaviviridae), a small enveloped flavivirus with an unsegmented positive-stranded RNA genome, is the most prominent member of the mammalian group of tick-borne flaviviruses. TBEV, originally isolated in 1937, is identified as Orthoflavivirus encephalitidis now. …”
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Advancing immunosuppression in liver transplantation: the role of regulatory T cells in immune modulation and graft tolerance by Ji Won Han, Su-Hyung Park

“…Emerging strategies, including the use of low-dose calcineurin inhibitors with mammalian target of rapamycin inhibitors, adoptive Treg therapy, and low-dose IL-2, aim to enhance Treg function while providing sufficient immunosuppression. …”
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Maternal Vitamin B12 in Pregnancy and Placental Development by Amrita Arcot, Rachel E. Walker, Kelly Gallagher, Kevin C. Klatt, Alison D. Gernand

“…Vitamin B12, or cobalamin, is an essential nutrient required for diverse physiological functions secondary to its role as a critical cofactor for two mammalian enzymes, methionine synthase and methylmalonyl-CoA mutase. …”
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Genes and light: many years later by A. O. Ruvinsky

“…Belyaev and discusses his studies of photoperiodic changes on mammalian reproduction and embryonic development in the realm of modern data. …”
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Reversion of Hormone Treatment Resistance with the Addition of an mTOR Inhibitor in Endometrial Stromal Sarcoma by J. Martin-Liberal, C. Benson, C. Messiou, C. Fisher, I. Judson

“…Hormone treatment is widely used in ESS but primary or acquired resistance is common. The mammalian target of rapamycin (mTOR) pathway has been suggested to play a key role in the mechanisms of hormone resistance. …”
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Kilohertz electrical stimulation evokes robust cellular responses like conventional frequencies but distinct population dynamics by Cara R. Ravasio, Krishnakanth Kondabolu, Samuel Zhou, Eric Lowet, Erynne San Antonio, Rebecca A. Mount, Sukhneet K. Bhogal, Xue Han

“…However, it is unknown how kilohertz stimulation influences neural activity in the mammalian brain. Using cellular calcium imaging in awake mice, we demonstrate that intracranial stimulation at 1 kHz evokes robust responses in many individual neurons, comparable to those induced by conventional 40 and 140 Hz stimulation in both the hippocampus and sensorimotor cortex. …”
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Functional significance of some common oxytocin receptor SNPs involved in complex human traits by Suk Ling Ma, Michael Thomas Bowen, Mark R. Dadds

“…Increasing cooperation between behavioral and biological scientists is needed to bridge the gap between human trait and functional biological studies to improve our understanding of oxytocin and other important mammalian neuroendocrine processes.…”
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REV7: a small but mighty regulator of genome maintenance and cancer development by Lara R. Maggs, Mitch McVey

“…Additionally, REV7 supports genome integrity by directing double-strand break repair pathway choice as part of the recently characterized mammalian shieldin complex. Given that genome instability is a hallmark of cancer, it is unsurprising that REV7, with its numerous genome maintenance roles, is implicated in multiple malignancies, including ovarian cancer, glioma, breast cancer, malignant melanoma, and small-cell lung cancer. …”
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The Role of mTOR Inhibitors in Liver Transplantation: Reviewing the Evidence by Goran B. Klintmalm, Björn Nashan

“…Clearly there is a need for better immunosuppressive agents that maintain similar rates of efficacy and renal function whilst minimizing adverse effects. The mammalian target of rapamycin (mTOR) inhibitors with a mechanism of action that is different from other immunosuppressive agents has the potential to address some of these issues. …”
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Protocol for the purification and analysis of nuclear UFMylated proteins by Pudchalaluck Panichnantakul, Marlene Oeffinger

“…Here, we present a technique to isolate nuclei and purify UFMylated proteins under denaturing non-reducing conditions from commonly used mammalian cell line models such as hTERT-RPE1, HEK293, U2OS, and HCT116 cells. …”
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Conditional Inactivation of HIF-1 Using Intrabodies by Arjan J. Groot, Eelke H. Gort, Elsken van der Wall, Paul J. van Diest, Marc Vooijs

“…Conditional expression of HIF intrabodies in mammalian cells interfered with binding to pVHL and inhibited hypoxia induced activation of endogenous target genes. …”
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Estimating the proportion of beneficial mutations that are not adaptive in mammals. by Thibault Latrille, Julien Joseph, Diego A Hartasánchez, Nicolas Salamin

“…These fitness landscapes have allowed us to predict the fitness effect of polymorphisms found in 28 mammalian populations. Using methods that quantify selection at the population level, we have confirmed that beneficial non-adaptive mutations are indeed positively selected in extant populations. …”
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Adult Hippocampal Neurogenesis in Alzheimer’s Disease: An Overview of Human and Animal Studies with Implications for Therapeutic Perspectives Aimed at Memory Recovery by Stefano Farioli-Vecchioli, Valentina Ricci, Silvia Middei

“…The mammalian hippocampal dentate gyrus is a niche for adult neurogenesis from neural stem cells. …”
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