Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses by Fanping Wang, Yanhua Li, Can Yang, Yonghui Mu, Yan Wang, Wei Zhang, Yonghui Yang, Chen Chen, Shijun Song, Zhifa Shen, Wenjun Wang, Junpeng Li, Jingjing Zhai, Kang Guo, Ruili Sun, Lili Yu, Mingyong Wang

“…Whether MBL can alter PGN-elicited cellular responses through TLR2 in macrophages is still unknown, and possible mechanism underlying it should be investigated. …”
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Development and utilization of Treponema pallidum expressing green fluorescent protein to study spirochete-host interactions and antibody-mediated clearance: expanding the toolbox... by Kristina N. Delgado, Crystal F. Vicente, Christopher M. Hennelly, Farhang Aghakhanian, Jonathan B. Parr, Kevin P. Claffey, Justin D. Radolf, Kelly L. Hawley, Melissa J. Caimano

“…We then used the GFP+ strain to visualize TPA interactions with host cells during co-cultivation in vitro, within infected rabbit testes, and following opsonophagocytosis by murine bone marrow-derived macrophages. The development of fluorescent strain also enabled us to develop a flow cytometric-based assay to assess antibody-mediated damage to the spirochete’s fragile outer membrane (OM), demonstrating dose-dependent growth inhibition and OM disruption in vitro. …”
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Targeted intra-tumoral hyperthermia using uniquely biocompatible gold nanorods induces strong immunogenic cell death in two immunogenically ‘cold’ tumor models by Barry E. Kennedy, Erin B. Noftall, Cheryl Dean, Alexander Roth, Kate N. Clark, Darren Rowles, Kulbir Singh, Len Pagliaro, Carman A. Giacomantonio, Carman A. Giacomantonio, Carman A. Giacomantonio

“…By 48 hours, CD45+ immune cell levels were increased, including increased levels of immunosuppressive M2 macrophages. While THT led to innate immune cell stimulations highlighted by gene expression upregulation in the STING cGAS pathway and enhanced M1 and dendritic cell levels, tumor regrowth was observed within six days post-treatment. …”
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Double-targeted liposomes coated with matrix metallopeptidase-2-responsive polypeptide nanogel for chemotherapy and enhanced immunotherapy against cervical cancer by Zhimin Bai, Yibo Yang, Zutong Cui, Wenming Liang, Xin Zhang, Zihan Zhang, Jianming Sun, Zhiwei Liu, Kun Li, Ming Shi, Jian Li

“…The second, a mannosyl-modified cationic liposome (CL), is loaded with Cytosine phosphate guanine (CpG) oligodeoxynucleotides to specifically target M2 phenotype macrophages, reversing their tumor-associated phenotype (TAM) and activating immune cytokines to promote tumor destruction. …”
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Spatial and single-cell transcriptomics reveal cellular heterogeneity and a novel cancer-promoting Treg cell subset in human clear-cell renal cell carcinoma by Lijuan Wang, Qian He, Weijun Qin, Xiyu Song, Yumeng Zhu, Wenwen Geng, Jianhua Jiao, Hongjiao Liu, Ruo Chen, Xiuxuan Sun, Jiejie Geng, Zhinan Chen

“…They were colocalized with MRC1+FOLR2+ tumor-associated macrophages (TAMs) at the tumor-normal interface to form a positive feedback loop, maintaining a synergistic procarcinogenic effect. …”
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Stimulation of Alpha1-Adrenergic Receptor Ameliorates Cellular Functions of Multiorgans beyond Vasomotion through PPARδ by Yong-Jik Lee, Hyun Soo Kim, Hong Seog Seo, Jin Oh Na, You-Na Jang, Yoon-Mi Han, Hyun-Min Kim

“…It also activated those energetic molecules and mitochondrial oxidative phosphorylation with cardiomyocytes, endothelial cells, adipocytes, macrophages, and hepatic cells and affected their relevant cell-specific biological functions. …”
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The mcpC mutant of Salmonella enteritidis exhibits attenuation and confers both immunogenicity and protective efficacy in mice by Lu Zhang, Li Chen, Xuqiang Zhang, Yang Li, Qingfeng Zheng, Yun Li, Ning Li, Qiumei Shi, Yanying Zhang, Tonglei Wu

“…However, ΔmcpC showed significantly reduced survival under acidic, alkaline, thermal, and oxidative stress conditions; markedly diminished motility; weakened invasion of Caco-2 cells; and reduced intracellular survival in RAW264.7 macrophages. The LD50 of ΔmcpC increased by 30-fold, and the expression levels of certain virulence genes were significantly downregulated. …”
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29 m6A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation – 102 Transcriptomic Dataset... by Ming Liu, Keman Xu, Fatma Saaoud, Ying Shao, Ruijing Zhang, Yifan Lu, Yu Sun, Charles Drummer, Li Li, Sheng Wu, Satya P. Kunapuli, Gerard J. Criner, Jianxin Sun, Huimin Shan, Xiaohua Jiang, Hong Wang, Xiaofeng Yang

“…We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA methylation (epitranscriptomic) regulators (m6A-RMRs) in 41 diseases and cancers and made significant findings: (1) a few m6A-RMRs were upregulated; and most m6A-RMRs were downregulated in sepsis, acute respiratory distress syndrome, shock, and trauma; (2) half of 29 m6A-RMRs were downregulated in atherosclerosis; (3) inflammatory bowel disease and rheumatoid arthritis modulated m6A-RMRs more than lupus and psoriasis; (4) some organ failures shared eight upregulated m6A-RMRs; end-stage renal failure (ESRF) downregulated 85% of m6A-RMRs; (5) Middle-East respiratory syndrome coronavirus infections modulated m6A-RMRs the most among viral infections; (6) proinflammatory oxPAPC modulated m6A-RMRs more than DAMP stimulation including LPS and oxLDL; (7) upregulated m6A-RMRs were more than downregulated m6A-RMRs in cancer types; five types of cancers upregulated ≥10 m6A-RMRs; (8) proinflammatory M1 macrophages upregulated seven m6A-RMRs; (9) 86% of m6A-RMRs were differentially expressed in the six clusters of CD4+Foxp3+ immunosuppressive Treg, and 8 out of 12 Treg signatures regulated m6A-RMRs; (10) immune checkpoint receptors TIM3, TIGIT, PD-L2, and CTLA4 modulated m6A-RMRs, and inhibition of CD40 upregulated m6A-RMRs; (11) cytokines and interferons modulated m6A-RMRs; (12) NF-κB and JAK/STAT pathways upregulated more than downregulated m6A-RMRs whereas TP53, PTEN, and APC did the opposite; (13) methionine-homocysteine-methyl cycle enzyme Mthfd1 downregulated more than upregulated m6A-RMRs; (14) m6A writer RBM15 and one m6A eraser FTO, H3K4 methyltransferase MLL1, and DNA methyltransferase, DNMT1, regulated m6A-RMRs; and (15) 40 out of 165 ROS regulators were modulated by m6A eraser FTO and two m6A writers METTL3 and WTAP. …”
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Alleviating Tumor Hypoxia and Immunosuppression via Sononeoperfusion: A New Ally for potentiating anti-PD-L1 blockade of solid Tumor by Guoliang Yang, Hui Li, Jiabei Yin, Lei Yao, Jun Yang, Jiawei Tang, You Wu, Meng Zhou, TingTing Luo, Yi Zhang, Jing Zhang, Xuezhi Yang, XiaoXiao Dong, Zheng Liu, Ningshan Li

“…Moreover, the sononeoperfusion effect reduced the accumulation of immunosuppressive cells, such as regulatory T cells (Tregs), myeloid-derived suppressor cells (MDSCs) and M2-like tumor-associated macrophages (M2-TAMs), and decreased the production of immune inhibitory factors like transforming growth factor-β1 (TGF-β1), interleukin 10 (IL-10), chemoattractant chemokines CC-chemokine ligand 22 (CCL22), CCL28, adenosine and lactate within tumors. …”
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Previous Exercise Training Reduces Markers of Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Female Rats by Liliany Souza de Brito Amaral, Cláudia Silva Souza, Rildo Aparecido Volpini, Maria Heloisa Massola Shimizu, Ana Carolina de Bragança, Daniele Canale, Antonio Carlos Seguro, Terezila Machado Coimbra, Amélia Cristina Mendes de Magalhães, Telma de Jesus Soares

“…At the end of the training protocol, we evaluated the following: glycosuria, body weight gain, plasma, renal and urinary levels of nitric oxide and thiobarbituric acid reactive substances, renal glutathione, and immunolocalization of lymphocytes, macrophages, and nuclear factor-kappa B (NF-κB/p65) in the renal cortex. …”
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Adaptation for Staphylococcus aureus to hosts via insertion mutation in the accessory gene regulator agrC gene: decreased virulence and enhanced persistence capacity by Jiawei Chen, Yun Wu, Ying Zhu, Li Zhang, Yingchun Xu, Yali Liu

“…Experimental findings showed that strain 23B had decreased hemolytic activity, lower cytotoxicity against human alveolar epithelial A549 cells and in the Galleria mellonella model, and a reduced ability to survive intracellularly after infecting macrophages, in comparison to 23H. Conversely, 23B exhibited enhanced biofilm formation, greater adherence to A549 cells, and increased persistence in the face of vancomycin and daptomycin treatment. …”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…A noteworthy suppression was observed in the immune microenvironment in nodules, revealed by the expression of macrophage, neutrophils, and NK cells. Furthermore, minor nodules exhibited a modestly elevated expression of macrophages compared to major nodules. …”
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A Study on the Inflammatory Response of the Brain in Neurosyphilis by Qiyu Zhang, Jie Ma, Jia Zhou, Hanlin Zhang, Mansheng Li, Huizi Gong, Yujie Wang, Heyi Zheng, Jun Li, Ling Leng

“…Moreover, monocyte‐derived macrophages are key cells involved in the inflammatory response to Tp in the CNS of NS patients. …”
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Novel combination strategy of high intensity focused ultrasound (HIFU) and checkpoint blockade boosted by bioinspired and oxygen-supplied nanoprobe for multimodal imaging-guided ca... by Ying Zhou, Chen Cheng, Qi Wang, Min Zheng, Pan Li, Yang Zhou, Yuting Cao, Li Wan, Xun Guo, Xiaohong Lin, Rui Tang, Hongye He, Nianhong Wu, Qiaoqi Chen, Yaqin Hu, Xialin Xiong, Faqi Li

“…At the same time, M@P-SOP released oxygen to alleviate the tumor hypoxic environment, repolarizing the protumor M2-type macrophages into antitumor M1-type. With concurrent anti-PD-L1 treatment, the antitumor immune response was further amplified to the whole body, and the growth of mimic distant tumor was effectively suppressed.Conclusions Our findings offer a highly promising HIFU synergist for effectively ameliorating acoustic and hypoxia environment, eventually inhibiting tumor growth and metastasis by stimulating host’s antitumor immunity under HIFU ablation, especially in synergizing with PD-L1 antibody immunotherapy.…”
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Deciphering the generation of heterogeneity in esophageal squamous cell carcinoma metastasis via single-cell multiomics analysis by Kaiwen Sheng, Jun Chen, Ruitang Xu, Haoqiang Sun, Ran Liu, Yongjie Wang, Wenwen Xu, Jiao Guo, Miao Zhang, Shuai Liu, Juan Lei, Yawen Sun, Yang Jia, Dianhao Guo

“…Furthermore, we observed that chromatin accessibility promoted the differentiation of tumor-associated macrophages (TAMs) and cancer associated fibroblasts (CAFs). …”
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KMO-driven metabolic reconfiguration and its impact on immune cell infiltration in nasopharyngeal carcinoma: a new avenue for immunotherapy by Nijun Chen, Yuan Zong, Chen Yang, Lei Li, Yang Yi, Jiawen Zhao, Xiaoyu Zhao, Xianfei Xie, Xingmei Sun, Ning Li, Liting Jiang

“…Importantly, KMO levels positively correlated with the infiltration of various immune cell populations, including B cells, T cells, and macrophages, as well as inflammatory signatures. Further investigation indicated that individuals with elevated KMO expression may exhibit heightened sensitivity to immune checkpoint blockade (ICB) therapy compared to those with lower KMO expression. …”
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Exosome proteomic analyses identify inflammatory phenotype and novel biomarkers in African American prostate cancer patients by Gati K. Panigrahi, Prakash P. Praharaj, Hiroki Kittaka, Asit R. Mridha, Olen M. Black, Rakesh Singh, Roger Mercer, Adrie van Bokhoven, Kathleen C. Torkko, Chapla Agarwal, Rajesh Agarwal, Zakaria Y. Abd Elmageed, Hariom Yadav, Santosh K. Mishra, Gagan Deep

“…Interestingly, African American PCa E006AA‐hT cells secreted exosomes strongly induced a proinflammatory M2‐phenotype in macrophages and showed calcium response on sensory neurons, suggesting a neuroinflammatory response. …”
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Gene signatures derived from transcriptomic-causal networks stratify colorectal cancer patients for effective targeted therapy by Akram Yazdani, Heinz-Josef Lenz, Gianluigi Pillonetto, Raul Mendez-Giraldez, Azam Yazdani, Hanna Sanoff, Reza Hadi, Esmat Samiei, Alan P. Venook, Mark J. Ratain, Naim Rashid, Benjamin G. Vincent, Xueping Qu, Yujia Wen, Michael Kosorok, William F. Symmans, John Paul Y. C. Shen, Michael S. Lee, Scott Kopetz, Andrew B. Nixon, Monica M. Bertagnolli, Charles M. Perou, Federico Innocenti

“…These signatures are highly associated with immune features, including macrophages, cytotoxicity, and wound healing. Furthermore, the corresponding proteins encoded by the genes within the signatures interact with each other and are functionally related. …”
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Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma by Yan Liao, Junkai Chen, Hao Yao, Ting Zheng, Jian Tu, Weidong Chen, ZeHao Guo, Yutong Zou, Lili Wen, Xianbiao Xie

“…SLC7A1 also regulate interactions between tumor cells and macrophages, as well as modulate macrophage function through multiple pathways. …”
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New evidence on the nephrotoxicity of fine particulate matter: Potential toxic components from different emission sources by Fu-Jen Cheng, Chien-Er Huang, Pei-Shih Chen, Yu-Lun Tseng, Chung-Shin Yuan, Ching-Shu Lai

“…To assess the impact of different PM2.5 components on inflammatory responses, a study was conducted by exposing the murine macrophage cell line (RAW 264.7). The study used a chelating resin to remove the influence of heavy metals from the water extract and employed a Toll-like receptor 4 (TLR4) antagonist to eliminate the effects of endotoxin, thereby evaluating the cellular inflammatory responses induced by various PM2.5 components. …”
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