Effects of Long-Term Serum Starvation on Autophagy, Metabolism, and Differentiation of Porcine Skeletal Muscle Satellite Cells by Yi Wang, Juan Gao, Bojun Fan, Yuemin Hu, Yuefei Yang, Yajie Wu, Jiaqiao Zhu, Junwei Li, Feng Li, Huiming Ju

“…The results showed that long-term serum starvation induced autophagy through the AMPK/mTOR signaling pathway, accelerated cell metabolism and apoptosis, exacerbated reactive oxygen species accumulation, and inhibited myogenic and adipogenic differentiation of SMSCs. …”
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5-HT7R enhances neuroimmune resilience and alleviates meningitis by promoting CCR5 ubiquitination by Zhenfang Gao, Yang Gao, Yuxiang Li, Jie Zhou, Ge Li, Shun Xie, Ruiyan Jia, Lanying Wang, Ziying Jiang, Meng Liang, Chunxiao Du, Yaqiong Chen, Yinji Liu, Lin Du, Cong Wang, Shuaijie Dou, Zhonglin Lv, Lubin Wang, Renxi Wang, Beifen Shen, Zhiding Wang, Yunfeng Li, Gencheng Han

“…Mechanistically, the Fluoxetine-5-HT7R axis induces proteasome-dependent degradation of CCR5 via mTOR signaling, and then recruits STUB1, an E3 ubiquitin ligase, to initiate K48-linked polyubiquitination of CCR5 at K138 and K322, promotes its proteasomal degradation. …”
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Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese by Jin Yu, Ichraf Mabrouk, Qiuyuan Liu, Yuxuan Zhou, Yupu Song, Jingyun Ma, Fengshuo Liu, Xiangman Hu, Zhiyi Yang, Yuxia Zeng, Jingtao Hu, Yongfeng Sun

“…These DEGs were enriched in Gene Ontology (GO) terms related to the microtubule cytoskeleton, cytoskeleton, cell adhesion, and biological adhesion and the KEGG enrichment analysis suggested that the DEGs were mainly enriched in “MAPK signaling pathway”, “Wnt signaling pathway”, “Calcium signaling pathway”, “Focal adhesion”, “Neuroactive ligand-receptor interaction”, “Melanogenesis”, “mTOR signaling pathway”, “ECM-receptor interaction”, and “TGF-beta signaling pathway” in the two comparison groups. …”
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RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia by Tim Kong, Angelo B. A. Laranjeira, Christopher T. Letson, LaYow Yu, Shuyang Lin, Jared S. Fowles, Daniel A. C. Fisher, Sherwin Ng, Wei Yang, Fan He, Minyoung Youn, Kailen Mark, Ana San Jose, Jingxian Liu, Alexander B. Kim, Maggie J. Cox, Mary C. Fulbright, Aarthi Jayanthan, Gerrit Los, Stacey L. Rentschler, Li Ding, Kathleen M. Sakamoto, Sandra E. Dunn, Grant A. Challen, Stephen T. Oh

“…Supported by mass cytometry (CyTOF) profiling, we reveal aberrant networks of PI3K/AKT/mTOR signalling and NFκB-mediated hyper-inflammation. …”
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Monocyte eukaryotic initiation factor 2 signaling differentiates 17-hydroxy-docosahexaenoic acid levels and pain by Peter R.W. Gowler, Asta Arendt-Tranholm, James Turnbull, Rakesh R. Jha, David Onion, Tony Kelly, Afroditi Kouraki, Paul Millns, Sameer Gohir, Susan Franks, David A. Barrett, Ana M. Valdes, Victoria Chapman

“…QIAGEN ingenuity pathway analysis identified the top ranked canonical biological pathway to be eukaryotic initiation factor 2 (EIF2) signaling (lower activation level in the low 17-HDHA-high pain group compared to the high 17-HDHA-low pain group (Z score −3)), followed by EIF4 and P70S6K signaling pathways and mTOR signaling. Our approach provides insight into the biological pathways contributing to the association between 17-HDHA and chronic osteoarthritis (OA) pain, identifying EIF2 signaling, with known roles in osteoclast differentiation, OA pathology, and pain, as a potential downstream target.…”
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Steroid hormone receptors, exome sequencing and treatment responsiveness of breast cancer patient-derived xenografts originated in a South American country by Gabriela Pataccini, Andrés Elia, Gonzalo Sequeira, Luisa Ambrosio, Marcela Coianis, Caroline A. Lamb, Paola A. Rojas, Paula Martínez Vázquez, Javier Burruchaga, Eunice Spengler, Silvia I. Vanzulli, Martin Abba, Claudia Lanari

“…BC-AR687 carried a PIK3CA (C420R) mutation and was sensitive to alpelisib and mTOR inhibitors. All PDX expressed AR with varying intensities. …”
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ALKBH5 suppresses gastric cancer tumorigenesis and metastasis by inhibiting the translation of uncapped WRAP53 RNA isoforms in an m6A-dependent manner by Ziqi Zheng, Feizhi Lin, Baiwei Zhao, Guoming Chen, Chengzhi Wei, Xiaojiang Chen, Runcong Nie, Ruopeng Zhang, Zhoukai Zhao, Zhiwei Zhou, Yuanfang Li, Weigang Dai, Yijia Lin, Yongming Chen

“…The lower level of WRAP53 disrupts the interaction between USP6 and RALBP1 protein, promoting RALBP1 degradation and thereby suppressing the PI3K/Akt/mTOR signaling cascade, ultimately attenuating the progression of GC. …”
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Molecular insight into reproductive toxicity and transgenerational effects of Cadmium exposure on Drosophila melanogaster by Yi Pan, Ke Fan, Linhao Zong, Yantao Luo, Xin Ni, Dong Chen, Jinjun Qian, Fei Ma, Jie Cheng, Miao Guan

“…Employing dose-response analysis, Wnt signaling pathway and mTOR signaling pathway were identified as early molecular responses to Cd-induced toxicity. …”
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Accumulation of advanced oxidation protein products aggravates bone-fat imbalance during skeletal aging by Yu-Sheng Huang, Jia-Wen Gao, Rui-Feng Ao, Xin-Yu Liu, Di-Zheng Wu, Jun-Long Huang, Chen Tu, Jing-Shen Zhuang, Si-Yuan Zhu, Zhao-Ming Zhong

“…The antioxidant NAC, after scavenging ROS, ameliorated the AOPPs-induced lineage switch and senescence in MSCs by inhibiting the PI3K/AKT/mTOR pathway. Conclusion: Our findings revealed the involvement of AOPPs in age‐related switch between osteogenic and adipogenic differentiation, and illuminated a novel potential mechanism underlying bone-fat imbalance during skeletal aging. …”
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Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD by Shaowei Wang, Boyang Li, Jie Li, Zhiheng Cai, Cristelle Hugo, Yi Sun, Lu Qian, Julia TCW, Helena C. Chui, Dante Dikeman, Isaac Asante, Stan G. Louie, David A. Bennett, Zoe Arvanitakis, Alan T. Remaley, Bilal E. Kerman, Hussein N. Yassine

“…Immunofluorescence and immunoblotting analyses confirmed increased lipofuscin-stained lipids and ABCA1 expression in AD brains and an association with mTOR phosphorylation. Discovery proteomics identified caveolin-1, a sensor of cellular cholesterol accumulation, as a key promoter of ABCA1 endolysosomal trafficking. …”
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Knockout of the inhibitory receptor TIGIT enhances the antitumor response of ex vivo expanded NK cells and prevents fratricide with therapeutic Fc-active TIGIT antibodies by Dean A Lee, Jeremiah L Oyer, Md Faqrul Hasan, Tayler J Croom-Perez, Liza D Robles-Carrillo, Sanjana Kumar, Brendan W Andersen, Brian P Tullius, Alicja J Copik, Amanda R Campbell, Thomas A Dieffenthaller, Catherine A Cash, Jonathan E Eloriaga, Meisam Naeimi Kararoudi

“…TIGIT KO NK cells upregulated mTOR complex 1 (mTORC1) signaling and had better metabolic fitness with an increased basal glycolytic rate when co-cultured with cancer cells compared with WT NK cells. …”
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NPRL2 gene therapy induces effective antitumor immunity in KRAS/STK11 mutant anti-PD1 resistant metastatic non-small cell lung cancer (NSCLC) in a humanized mouse model by Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

“…Stable-expression of NPRL2 resulted in the downregulation of MAPK and AKT-mTOR signaling. Taken-together, NPRL2 gene-therapy induces antitumor activity on KRAS/STK11mt/aPD1R tumors through DC-mediated antigen-presentation and cytotoxic immune-cell activation.…”
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Immunosuppressive therapy and nutritional diseases of patients after kidney transplantation: a systematic review by Aleksandra Anna Kajdas, Marcin Kleibert, Anne Katrine Normann, Krzysztof Krasuski, Ditte Søndergaard Linde, Dorota Szostak-Węgierek

“…Finally, mammalian target of rapamycin (mTOR) inhibitors was associated with bone status and mycophenolate mofetil was linked to Vitamin B12 deficiencies. …”
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Prevention and management of cytomegalovirus infection and disease in kidney transplant: A consensus statement of the Transplantation Society of Taiwan by Mu-Chi Chung, Cheng-Hsu Chen, Shen-Shin Chang, Chih-Yuan Lee, Ya-Chung Tian, Mei-Yi Wu, Hsu-Han Wang, Chia-Cheng Yu, Teng-Wei Chen, Chien-Chang Kao, Chih-Yang Hsu, Yang-Jen Chiang, Ming-Ju Wu, Yen-Ta Chen, Mai-Szu Wu

“…Routine surveillance after prophylaxis, secondary prophylaxis after CMV disease treatment, and mTOR inhibitors for primary CMV prophylaxis were not recommended. …”
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Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation by Shi-Qing Mu, Jia-Jie Lin, Yu Wang, Li-Yun Yang, Sen Wang, Zhao-Yi Wang, An-Qi Zhao, Wen-Jun Luo, Zi-Qi Dong, Yu-Guang Cao, Ze-An Jiang, Si-Fan Wang, Shan-Hu Cao, Li Meng, Yang Li, Shu-Yan Yang, Shao-Guang Sun

“…Subsequently, the YTHDF2 protein promotes the degradation of mTOR mRNA by binding to the latter’s m6A modification sites. …”
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A General Framework for Closed Loop Negative Feedback Multivariable Physiological Control Systems: Existence, Uniqueness, and Stability of Homeostatic Equilibrium Points by Anna Procopio, Annarita Tedesco, Fabrizio Lo Regio, Giuseppe Cesarelli, Leandro Donisi, Carlo Ricciardi, Alessio Merola, Alfonso Maria Ponsiglione, Maria Romano, Francesco Montefusco, Carlo Cosentino, Francesco Amato

“…To this regard, in this paper, the existence, possible uniqueness and stability properties of the homeostatic equilibrium points of the general model are investigated; the theoretical framework is then illustrated through two real-world case-studies: (i) the PI3K/AKT/mTOR pathway nonlinear dynamics, a critical regulator of cellular growth, proliferation, and survival; (ii) the control mechanism of the neuromuscular stretch reflex, among the prime triggers implicated in postural control. …”
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Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target by Cèlia Dobaño-López, Juan García Valero, Ferran Araujo-Ayala, Ferran Nadeu, Fabien Gava, Carla Faria, Marine Norlund, Renaud Morin, Pascale Bernes-Lasserre, Fabian Arenas, Marta Grau, Cristina López, Irene López-Oreja, Neus Serrat, Ares Martínez-Farran, Lluís Hernández, Heribert Playa-Albinyana, Rubén Giménez, Silvia Beà, Elías Campo, Jean-Michel Lagarde, Armando López-Guillermo, Laura Magnano, Dolors Colomer, Christine Bezombes, Patricia Pérez-Galán

“…FL-PDLS recapitulates the most relevant B-cell transcriptional pathways present in FL-LN (proliferation, epigenetic regulation, mTOR, adaptive immune system, among others). The T cell compartment in the FL-PDLS preserves CD4 subsets (follicular helper, regulatory, and follicular regulatory), also encompassing the spectrum of activation/exhaustion phenotypes in CD4 and CD8 populations. …”
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Pre-operative DNA methylation marks as predictors of weight loss outcomes after sleeve gastrectomy by Guillermo Paz-López, Teresa M. Linares-Pineda, Andrés González-Jiménez, Raquel Sancho-Marín, Luis Ocaña-Wilhelmi, Francisco J. Tinahones, Sonsoles Morcillo, Carolina Gutiérrez-Repiso

“…KEGG enrichment analysis showed that pathways involved in the signalling of MAPK, Wnt, mTor, FoxO and AMPK, among others, were involved in weight loss trajectory.A stepwise logistic regression using the DMPs with an absolute Δβ >0.2 showed that higher methylation levels in the CpG sites cg02405213 (mapping to JAK2) (OR: 1.20098, [0.9586, 1.5044]) and cg01702330 (OR: 2.4426, [0.5761, 10.3567]), were shown to be associated with a higher probability of achieving >60 %EWL after sleeve gastrectomy, whereas higher methylation levels in the CpG site cg04863892 (mapping to HOXA5) were associated with lower probability of achieving >60 %EWL after sleeve gastrectomy (OR: 0.7966, [0.5637, 1.1259]). …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…It was further found by functional enrichment analysis, qRT-PCR, western blot (WB) and ELISA that MMP16 may play a regulatory role in GCs proliferation as well as steroid hormone secretion through the mTOR signaling pathway and PPAR signaling pathway. …”
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Maternal exposure to polystyrene nanoplastics during gestation and lactation caused fertility decline in female mouse offspring by Xiu Cheng, Yue Xue, Houpeng Wang, Zhangqiang Ma, Na Hu, Chenchen Zhang, Yu Gao, Ruihong Fan, Liaoliao Hu, Jia Li, Dalei Zhang, Jian Huang, Sitian Fang, Runting Xiao, Yuanqiao He, Tao Luo, Liping Zheng

“…This premature activation is likely due to the PS-NPs'induction of the AKT-FOXO3a signaling pathway by downregulating AMPK phosphorylation level and enhancing mTOR activity. Furthermore, a significant reduction in transzonal projections (TZPs) was noted in the ovaries of adult offspring mice in PS-NPs group. …”
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