Transcription factor RUNX1 regulates coagulation factor XIII-A (F13A1): decreased platelet-megakaryocyte F13A1 expression and clot contraction in RUNX1 haplodeficiency by Fabiola Del Carpio-Cano, Natthapol Songdej, Liying Guan, Guangfen Mao, Lawrence E. Goldfinger, Jeremy G.T. Wurtzel, Kiwon Lee, Michele P. Lambert, Mortimer Poncz, A. Koneti Rao

“…Results: Platelet F13A1 mRNA and protein were decreased in our index patient and in 2 siblings from an unrelated family with RHD. …”
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Endoplasmic reticulum stress in the salivary glands of patients with primary and associated Sjögren’s disease, and non-Sjögren’s sicca syndrome: a comparative analysis and the infl... by Graziela Vieira Cavalcanti, Fabiola Reis de Oliveira, Rafael Ferraz Bannitz, Natalia Aparecida de Paula, Ana Carolina Fragoso Motta, Eduardo Melani Rocha, John Chiorini, Hilton Marcos Alves Ricz, Denny Marcos Garcia, Maria Cristina Foss-Freitas, Luiz Carlos Conti de Freitas

“…Histopathological analysis and mRNA expression analysis of genes associated with ERS and UPR (PERK, XBP1, ATF-6, ATF-4, CANX, CALR, CHOP, and BIP) were performed on the samples. …”
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Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model by Tiantian Hu, Rui Chen, Yu Qian, Ke Ye, Xingyao Long, Kun-Young Park, Xin Zhao

“…The activities of GSH, GSH-Px, SOD, CAT and T-AOC in serum, brain and liver of soybean milk fermented by LF-HFY02 were significantly increased, while the content of MDA and the level of AGEs in hippocampal were significantly decreased compared with D-galactose induced group. Further more, the mRNA expression of GSH and SOD in mouse liver were obviously up-regulated by soybean milk fermented by LF-HFY02. …”
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Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice by Dongliang Chen, Yue Shen, Yue Shen, Fang Huang, Bo Huang, Shangfu Xu, Shangfu Xu, Lisheng Li, Lisheng Li, Jie Liu, Zheng Li, Xia Li

“…PCE restored the expression of AMPK, SIRT1, SREBP1 and PPAR-α both in mRNA and protein levels. RNAseq identified unique gene expression profiles in response to high-fat diet (HFD) compared to the PCE treatments. …”
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Exploring the relationship between sepsis and Golgi apparatus dysfunction: bioinformatics insights and diagnostic marker discovery by Wanli Ma, Xinyi Liu, Ran Yu, Jiannan Song, Lina Hou, Ying Guo, Hongwei Wu, Dandan Feng, Qi Zhou, Haibo Li

“…This study aims to explore the relationship between sepsis and the Golgi apparatus using bioinformatics, offering fresh insights into its diagnosis and treatment.MethodsTo explore the role of Golgi-related genes in sepsis, we analyzed mRNA expression profiles from the NCBI GEO database. …”
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Understanding the mechanisms of hypoxia-induced tissue damage in fish: The role of GasderminEa/b in Larimichthys crocea by Lin Wang, Shuaiyu Chen, Yongyang Wang, Weini Zhang, Yinnan Mu, Yudong Jia, Changtao Guan, Meng Xue, Xinhua Chen

“…Compared to LcGSDMEb, the expression of LcGSDMEa was higher in both mRNA and protein levels, thus exhibiting a stronger response during hypoxia stress. …”
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Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean by Liya Zhang, Jun Liu, Jiaqi Chen, Yanyan Zhang, Chao Qin, Xiangguang Lyu, Zhuang Li, Ronghuan Ji, Bin Liu, Hongyu Li, Tao Zhao

“…Abstract Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechanism regulating plant biological processes; however, its impact on shade avoidance remains unexplored. …”
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Establishment of <i>Echinococcus granulosus EgM123</i> Recombinant Gene Rabies Virus SRV₉ and Identification of Its Biological Characteristics by Yueqi Yang, Mengdan Hou, Guicheng Su, Xiaoyan Ma, Xiaohui Su, Kunlei Li, Songhan Liu, Luheng Xiao, Jingjing Yao, Jiahao Zhai, Xiaoying Wei, Yang Zhou, Qianqian Lai, Yuwei Dong, Jieyu Liu, Shaohua Zhai

“…The rescued virus-infected BSR cells were characterized by TEM, and the results indicated that bullet-like viral particles with an average size of 148.47 nm and a cyst structure were present in the cytoplasm of BSR cells; the expression levels of continuously cultivated 9th-, 10th-, 11th-, 12th-, and 13th-generation viruses were quantified by qRT-PCR, and the results showed that mRNA expression of the virus was upregulated. The LD₅₀ titer of suckling rats was measured to be 10^−1.4. …”
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Transcriptomic profiling of lncRNAs and mRNAs in a venous thrombosis mouse model by Risheng Hao, Haobo Li, Xincheng Li, Jixiang Liu, Xiaofan Ji, Hong Zhang, Zhu Zhang, Peiran Yang, Zhenguo Zhai

“…Enrichment analyses revealed significant pathways, including HIF-1α signaling, glycolysis/gluconeogenesis, and platelet activation. A lncRNA-miRNA-mRNA network highlighted key regulatory interactions. …”
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Black carp RNF115 restricts IRF3/7-mediated antiviral signaling in innate immunity by Yixuan He, Qun Wang, Lili Xiao, Hui Wu, Jun Xiao, Jun Zou, Hao Feng

“…Overexpression of RNF115 impaired interferon (IFN) and the related interferon-stimulated gene (ISG) mRNA expression, while upregulating SVCV replication. …”
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Salvianolic Acid D Alleviates Cerebral Ischemia-Reperfusion Injury by Suppressing the Cytoplasmic Translocation and Release of HMGB1-Triggered NF-κB Activation to Inhibit Inflammat... by Wen Zhang, Junke Song, Wan Li, Dewen Kong, Yu Liang, Xiaoyue Zhao, Guanhua Du

“…Cytokines and cytokine mRNA, including TNF-α, IL-1β, and IL-6, were studied via ELISA and PCR methods. …”
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Dual-release hydrocortisone treatment improves serum and peripheral blood mononuclear cell inflammatory and immune profiles in patients with autoimmune primary adrenal insufficienc... by Laura Tomasello, Antonina Coppola, Giuseppe Pizzolanti, Giuseppe Pizzolanti, Carla Giordano, Giorgio Arnaldi, Valentina Guarnotta

“…After 12 months of DR-HC treatment, we found significantly lower IL-6 (p = 0.019), IL-17A (p = 0.046), COX-2 (p &lt; 0.001), HSP-70 (p = 0.006), and TXN-1 (p = 0.008) and higher PD-L1 (p &lt; 0.001) and IDO (p &lt; 0.001) mRNA values compared to baseline. After 12 months of DR-HC treatment, a significant increase in CD4+ T cells (p = 0.012), PD-L1 (p = 0.003), and IDO (p &lt; 0.001) and a decrease in CD8+ T cells (p &lt; 0.001), IL-6 (p = 0.003), IL-17A (p = 0.0014), COX-2 (p &lt; 0.001), HSP-70 (p = 0.005), and TXN-1 (p = 0.0008), as well as a significantly higher conversion in the CD4+/CD8+ ratio (p = 0.033), were observed compared to conventional GCs.ConclusionsThe switch from conventional GCs to DR-HC treatment altered the T lymphocyte phenotype and CD4+/CD8+ ratio in a Treg-independent manner, inducing significant improvements in the immune and inflammatory profile in PAI.…”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…Additionally, among the significantly up-regulated cell cycle-related genes in the major nodules when compared with minor nodules, CCNE1 mRNA expression demonstrated significant correlation with poor prognosis in the lung cancer. …”
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Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses by Hongyu Yu, Shaoyuan Cui, Yan Mei, Qinggang Li, Lingling Wu, Shuwei Duan, Guangyan Cai, Hanyu Zhu, Bo Fu, Li Zhang, Zhe Feng, Xiangmei Chen

“…Polarization of naïve CD4+ T cells (Th0 cells) towards the Th1 phenotype was induced by coculture with activated mesangial cells, and the resulting Th1 cells showed increased mRNA and protein expression of inflammation-associated genes. …”
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Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide by Yazmin M. Flores-Martinez, Manuel A. Fernandez-Parrilla, Jose Ayala-Davila, David Reyes-Corona, Victor M. Blanco-Alvarez, Luis O. Soto-Rojas, Claudia Luna-Herrera, Juan A. Gonzalez-Barrios, Bertha A. Leon-Chavez, Maria E. Gutierrez-Castillo, Irma A. Martínez-Dávila, Daniel Martinez-Fong

“…During the acute phase of neuroinflammation, pro- and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-4, and IL-10) responded differentially at mRNA and protein level. Increased NO production and lipid peroxidation occurred at 168 h after LPS injection. …”
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Clinicopathological Significance of FOXO4 Expression and Correlation with Prx1 in Head and Neck Squamous Cell Carcinoma by Yunping Lu, Yajun Shen, Lingyu Li, Min Zhang, Min Wang, Lihua Ge, Jing Yang, Xiaofei Tang

“…By the analysis of Bioinformation Databases, there was a significant interaction of FOXO4 down expression to clinical tumor stages and pathological grades in the patients with HNSCC. Reduced mRNA and protein expression of FOXO4 were found to be significantly correlated with the poor overall survival (OS) of HNSCC patients. …”
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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders by Nincy Debeuf, Sahine Lameire, Manon Vanheerswynghels, Julie Deckers, Caroline De Wolf, Wendy Toussaint, Rein Verbeke, Kevin Verstaen, Hamida Hammad, Stijn Vanhee, Bart N Lambrecht

“…After immunization with Spike/CpG-motifs, mRNA vaccination or SARS-CoV-2 infection, CORSET8 T cells strongly proliferated and showed signs of T cell activation. …”
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G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration by Liana C. Boraas, Mengwei Hu, Pieter Martino, Lauren Thornton, Charles E. Vejnar, Gang Zhen, Longhui Zeng, Dylan M. Parker, Andy L. Cox, Antonio J. Giraldez, Xiaolei Su, Christine Mayr, Siyuan Wang, Stefania Nicoli

“…For instance, β-actin mRNA and its associated RNA-binding protein (RBP), ZBP1/IGF2BP1, are recruited to focal adhesions (FAs) to support localized β-actin synthesis, crucial for cell migration. …”
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Acquired vulnerability against EGF receptor inhibition in gastric cancer promoted by class I histone deacetylase inhibitor entinostat by Tamara Zenz, Robert Jenke, Denys Oliinyk, Sandra Noske, René Thieme, Tim Kahl, Ines Gockel, Florian Meier-Rosar, Achim Aigner, Thomas RH Büch

“…Methods: We investigated entinostat effects on EGFR and amphiregulin (AREG) expression in various cell line- and primary patient tumor-based in vitro, ex vivo and in vivo models, on the mRNA and protein level. Based on these results, a combined entinostat plus EGFR inhibitor erlotinib treatment in vitro and in vivo was studied. …”
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Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity by Yang Wang, Jianguo Xu, Wanchun You, Haitao Shen, Xiang Li, Zhengquan Yu, Haiying Li, Gang Chen

“…In experiment one, we found that the protein level of Rufy3 decreased and neuronal axon injury in the injured neurons but was rectified by LV-Rufy3 treatment. In experiment two, mRNA and protein levels of Rufy3 were downregulated in brain tissue and reached the lowest level at 24 h after SAH. …”
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