Stochastic demethylation and redundant epigenetic suppressive mechanisms generate highly heterogeneous responses to pharmacological DNA methyltransferase inhibition by Mie K. Jakobsen, Sofie Traynor, Aaraby Y. Nielsen, Christina Dahl, Mette Staehr, Simon T. Jakobsen, Maria S. Madsen, Rasmus Siersbaek, Mikkel G. Terp, Josefine B. Jensen, Christina B. Pedersen, Anup Shrestha, Jonathan R. Brewer, Pascal H. G. Duijf, Odd L. Gammelgaard, Henrik J. Ditzel, Alexei F. Kirkin, Per Guldberg, Morten F. Gjerstorff

“…The analysis was performed on breast cancer patient-derived xenograft tumors and cell lines, employing a comprehensive set of techniques, including targeted single-cell mRNA sequencing. Mechanistic insights were further gained through DNA methylation profiling and chromatin structure analysis. …”
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Lymphoma patients treated with anti-CD20 and chemotherapy display disconnected T and B cell responses to COVID-19 vaccine by Sylvain Lamure, Sylvain Lamure, Houria Hendel Chavez, Marie-Ghislaine de Goër de Herve, Luc-Matthieu Fornecker, Bernard Drenou, Caroline Jacquet, Fatiha Merabet, Milena Kohn, Florence Quélin, Angela Jackson, Sylvain Choquet, Rémy Duléry, Yassine Taoufik, Caroline Besson, Caroline Besson

“…We prospectively evaluated CD4, CD8 T-cell and serological responses to the COVID-19 mRNA vaccine in a cohort of patients treated for a B-cell lymphoma with anti-CD20 therapy. …”
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Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation by Sergio Mora-Castilla, Juan R. Tejedo, Rafael Tapia-Limonchi, Irene Díaz, Ana B. Hitos, Gladys M. Cahuana, Abdelkrim Hmadcha, Franz Martín, Bernat Soria, Francisco J. Bedoya

“…Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. …”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…A significant upregulation of atrogin-1 mRNA was observed at salinities of 16 ‰ and 24 ‰ compared to the control group. …”
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Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice by Youxiang Zhang, De-Hui Xiong, Yangyang Li, Guina Xu, Baoxin Zhang, Yang Liu, Shan Zhang, Qing Huang, Simin Chen, Fansheng Zeng, Jingyi Guo, Bin Li, Zhiqiang Qin, Zuping Zhang

“…In addition, the levels of the USP21 protein and mRNA in the liver and spleen of KO mice decreased. …”
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Water homeostasis gene expression in the kidney of broilers divergently selected for water conversion ratio by Kentu Lassiter, Loujain Aloui, Elizabeth S. Greene, Marciela Maqaeda, Travis Tabler, Sami Dridi, Robert F. Wideman, Jr., Sara Orlowski, Walter G. Bottje

“…Molecular analyses from Exp. 1 showed that the renal expression of arginine vasopressin (AVP), angiotensinogen (AGT), angiotensin II receptor type 1 and 2 (AT1/2), sodium-potassium ATPase subunit B1 (ATP1B1), and aquaporin 3 (AQP3) were upregulated in HWE compared to the LWE line. In contrast, mRNA expression of mesotocin receptor (MTR), AT1/2, AQP1/2, and occludin were significantly higher in females than in males. …”
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GLP‐1 Receptor Agonists Alleviate Diabetic Kidney Injury via β‐Klotho‐Mediated Ferroptosis Inhibition by Shasha Tian, Saijun Zhou, Weixi Wu, Yao lin, Tongdan Wang, Haizhen Sun, A‐Shan‐Jiang A‐Ni‐Wan, Yaru Li, Chongyang Wang, Xiaogang Li, Pei Yu, Yanjun Zhao

“…Notably, Smg treatment significantly increases the mRNA expression of KLB through the activation of the cyclic adenosine monophosphate (cAMP) signaling pathway, specifically through the phosphorylation of protein kinase A (PKA) and cAMP‐response element‐binding protein (CREB). …”
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Hesperidoside abolishes dichlorvos-mediated neurotoxicity in rats by suppressing oxidative stress, acetylcholinesterase inhibition, and NF-κB-p65/p53/caspase-3-mediated apoptosis by Adio J. Akamo, Adetutu O. Ojelabi, Oluwatobi T. Somade, Iyabode A. Kehinde, Adewale M. Taiwo, Boluwatife A. Olagunju, Mushafau A. Akinsanya, Adedayo A. Adebisi, Tobi S. Adekunbi, Abiola F. Adenowo, Florence Anifowose, Olufemi M. Ajagun-Ogunleye, Ofem E. Eteng, Jacob K. Akintunde, Regina N. Ugbaja

“…Results: HESP intervention remarkably (p < 0.05) mitigated DDVP-potentiated augmentations in cerebral concentrations of H₂O₂, NO, and malondialdehyde; impaired DDVP-induced decrease in cerebral GSH, GST, SOD, catalase, glutathione peroxidase, and acetylcholinesterase; significantly (p < 0.05) suppressed DDVP-invoked upregulation of mRNA expression of NF-κB-p65, p53, BAX, caspase-3, and TNF-α; and significantly (p < 0.05) revoked DDVP-incited downregulation of interleukin-10. …”
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Interferon-α promotes HLA-B-restricted presentation of conventional and alternative antigens in human pancreatic β-cells by Alexia Carré, Fatoumata Samassa, Zhicheng Zhou, Javier Perez-Hernandez, Christiana Lekka, Anthony Manganaro, Masaya Oshima, Hanqing Liao, Robert Parker, Annalisa Nicastri, Barbara Brandao, Maikel L. Colli, Decio L. Eizirik, Jahnavi Aluri, Deep Patel, Marcus Göransson, Orlando Burgos Morales, Amanda Anderson, Laurie Landry, Farah Kobaisi, Raphael Scharfmann, Lorella Marselli, Piero Marchetti, Sylvaine You, Maki Nakayama, Sine R. Hadrup, Sally C. Kent, Sarah J. Richardson, Nicola Ternette, Roberto Mallone

“…Here we characterize the HLA-I antigen presentation in resting and IFN-α-exposed β-cells and find that IFN-α increases HLA-I expression and expands peptide repertoire to those derived from alternative mRNA splicing, protein cis-splicing and post-translational modifications. …”
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Exploring biomarkers and molecular mechanisms of Type 2 diabetes mellitus promotes colorectal cancer progression based on transcriptomics by Simin Luo, Yuhong Zhu, Zhanli Guo, Chuan Zheng, Xi Fu, Fengming You, Xueke Li

“…External datasets confirmed from both mRNA and protein expression levels that low COX11 expression was significantly associated with poor CRC prognosis. …”
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RNA N6‐Methyladenosine‐Binding Protein YTHDFs Redundantly Attenuate Cancer Immunity by Downregulating IFN‐γ Signaling in Gastric Cancer by Dongjun Jang, Chanwoong Hwa, Seoyeon Kim, Jaeik Oh, Seungjae Shin, Soo‐Jin Lee, Jiwon Kim, Sang Eun Lee, Yoojin Yang, Dohee Kim, Seoho Lee, Hae Rim Jung, Yumi Oh, Kyunggon Kim, Hye Seung Lee, Joon‐Yong An, Sung‐Yup Cho

“…RNA immunoprecipitation sequencing revealed YTHDFs directly target interferon regulatory factor 1 (IRF1) mRNA, a master regulator of IFN‐γ signaling, leading to reduced RNA stability and consequent downregulation of IFN‐γ signaling. …”
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Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses by Fanping Wang, Yanhua Li, Can Yang, Yonghui Mu, Yan Wang, Wei Zhang, Yonghui Yang, Chen Chen, Shijun Song, Zhifa Shen, Wenjun Wang, Junpeng Li, Jingjing Zhai, Kang Guo, Ruili Sun, Lili Yu, Mingyong Wang

“…In this study, we found that MBL significantly attenuated PGN-induced inflammatory cytokine production, including TNF-α and IL-6, in PMA-stimulated THP-1 cells at both mRNA and protein levels. The expression of TLR2 was strongly induced by PGN stimulation. …”
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Identification of EARS2 as a Potential Biomarker with Diagnostic, Prognostic, and Therapeutic Implications in Colorectal Cancer by Wang L, Deng X, Tang J, Gong Y, Bu S, Li Z, Liao B, Ding Y, Dai T, Liao Y, Li Y

“…To investigate the mechanisms underlying EARS2 in CRC, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Gene Set Enrichment Analysis, and Protein-Protein Interaction analyses were performed, alongside the construction of miRNA-mRNA interaction networks. Additionally, the relationships between EARS2 and immune cell infiltration, as well as responses to drug therapy, were examined. …”
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The role of FAM171A2-GRN-NF-κB pathway in TBBPA induced oxidative stress and inflammatory response in mouse-derived hippocampal neuronal HT22 cells by Yi Wang, Jing Shan, Ling Zhang, Rui Wang, Meng-Yu Wu, Hong-Mei Li, Hai-Ming Xu

“…The results showed that TBBPA exposure could lead to an increase in the production of inflammation-related genes IL-6, iNOS, TGF-β1, COX2, and TNF-α in both HT22 cells and HT22-AD-model, intensifying the inflammatory response; it inhibits the mRNA expression of antioxidative enzymes genes Sod1, Cat, Gpx1, and Gsta1, resulting in reduced antioxidant enzyme activities of SOD, CAT, and GSH-Px/GPX. …”
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Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor by Junying Wang, Mengping Chen, Jinxing Jiang, Yike Wan, Xin Li, Minyue Zhang, Fei Xiao, Lu Zhong, Hua Zhong, Zhaoyu Qin, Jian Hou

“…Methods We assessed XPO1 protein and mRNA levels using western blotting and RT-qPCR. Cycloheximide (CHX) chase assays and degradation blockade assays were used to determine the pathway of XPO1 degradation induced by SEL. …”
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DNMT3a Downregulation Ttriggered Upregulation of GABAA Receptor in the mPFC Promotes Paclitaxel‐Induced Pain and Anxiety in Male Mice by Lixia Tian, Xu‐Hui Li, Yu‐Long Zhao, Hui‐Yuan Yi, Xue‐Ru Liu, Rongrong Yao, Xue‐Mei Hou, Xuan Zhu, Fu‐Quan Huo, Tao Chen, Lingli Liang

“…In this study, systemic administration of PTX leads to a decrease in the expression of the DNA methyltransferase DNMT3a, while concurrently upregulating the expression of Gabrb1 mRNA and its encoded GABAARβ1 protein in the medial PFC (mPFC) of male mice. …”
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Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study by Guadalupe Ávalos-Navarro, Luis A. Bautista-Herrera, Asbiel Felipe Garibaldi-Ríos, Ramiro Ramírez-Patiño, Marisol Gutiérrez-García, Perla Briseño-Álvarez, Luis Felipe Jave-Suárez, Emmanuel Reyes-Uribe, Martha Patricia Gallegos-Arreola

“…Furthermore, <i>SERPINA1</i> mRNA overexpression in BC is linked to a protective effect.…”
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Altered zygotic gene expression caused by sperm with Tdrd6 variants disrupts early embryonic development by Zhijie Hu, Yuqi Zhang, Renfei Cai, Qiuju Chen, Haiyan Guo, Danjun Li, Weidong Lin, Hongxi He, Haibo Wu, Yali Liu, Bin Li, Qianwen Xi, Hongyuan Gao, Jian Zhang, Qifeng Lyu, Yanping Kuang, Xuefeng Lu

“…Additionally, our research revealed that OAD influences the expression of multitude of genes at the 2‐pronuclear (2PN) stage, with the Mos gene playing a pivotal role in early embryonic arrest. The injection of Mos mRNA can mitigate this arrest. To our knowledge, this is the first study to show that sperm‐related OAD affects gene expression at the 2PN stage and elucidate how AOA overcomes male factor‐derived embryonic arrest, enabling successful pregnancies and live births.…”
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Cis‐Regulation of an m6A Eraser by an Insertion Variant Associated with Survival of Patients With Non‐Small Cell Lung Carcinoma by Lei Cheng, Qiangsheng Hu, Yanan Wang, Wei Nie, Haijiao Lu, Bo Zhang, Genming Zhao, Shiyun Ding, Feng Pan, Yinchen Shen, Runbo Zhong, Ruoxin Zhang

“…Then, ALKBH5 upregulated FBXL5 expression by m6A demethylation, which is dependent on the ALKBH5 H204 amino acid site and specific m6A sites on FBXL5 mRNA. Finally, the ALKBH5‐FBXL5 axis reduces intracellular reactive oxygen species levels, leading to PI3K/AKT and NF‐kB pathway inhibition and consequently suppresses NSCLC proliferation and metastasis in vitro and in vivo. …”
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High-fat diet-induced liver injury in Channa argus through lipid metabolism, anti-oxidative status, apoptosis, and inflammation by Jiaxin Tian, Ying Li, Jiwu Wan, Zhinan Yang, Guiqin Wang

“…At 90 days, the L22 group exhibited a significant upregulation of mRNA expression levels for liver inflammatory factors, including interleukin-1β (IL-1β), IL-8, IL-10, and tumor necrosis factor-α (TNF-α). …”
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