Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress by Ping Han, Jianming Chen, Zhennan Sun, Shengjie Ren, Xubo Wang

“…Abstract Background Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels. The most crucial step in obtaining accurate results of qRT-PCR is to select suitable reference genes. …”
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The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women by Huihui Li, Xingyu Yan, Man Yang, Mei Liu, Shan Tian, Mengru Yu, Wei-Ping Li, Cong Zhang

“…PTPRK is primarily localized in placental trophoblast cells. The mRNA and protein levels of PTPRK in PE were significantly higher than those in controls. …”
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Effects of Rosa roxburghii & edible fungus fermentation broth on immune response and gut microbiota in immunosuppressed mice by Dechang Xu, Jielun Hu, Yadong Zhong, Yanli Zhang, Wenting Liu, Shaoping Nie, Mingyong Xie

“…REFB administration also up-regulated the expression of IL-4, INF-γ, TNF-α, T-bet and GATA-3 mRNA in small intestine. Furthermore, administration of REFB modulated gut microbiota composition and increased the relative abundance of beneficial genus, such as Bacteroides. …”
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Comparative analysis of the whole transcriptome landscapes of muscle and adipose tissue in Qinchuan beef cattle by Ju Qiu, Zheng Ma, Zhipeng Hong, Xu Yin, Yun Chen, Hafiz Qadeer Ahmed, Linsen Zan, Anning Li

“…Based on the competing endogenous RNA (ceRNA) regulatory mechanism, we constructed a lncRNA/circRNA-miRNA-mRNA network consisting of 6 circRNAs, 5 lncRNAs, 6 miRNAs and 27 mRNAs. …”
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Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis by Mónica Kurte, Javiera Bravo-Alegría, Alexander Torres, Vania Carrasco, Cristina Ibáñez, Ana María Vega-Letter, Catalina Fernández-O’Ryan, Carlos E. Irarrázabal, Fernando E. Figueroa, Rodrigo A. Fuentealba, Claudia Riedel, Flavio Carrión

“…Interestingly, regardless of time administration, MSCs significantly reduced IL-6 and also T-bet, RORγT, and Foxp3 mRNA levels in brain samples of EAE mice. The downregulation of IL-6 could restore the well-functioning of the blood-brain barrier of EAE mice, correlated with a decreased number of brain infiltrating leukocytes. …”
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Clinical Characteristics of Aldosterone- and Cortisol-Coproducing Adrenal Adenoma in Primary Aldosteronism by Lu Tang, Xintao Li, Baojun Wang, Xin Ma, Hongzhao Li, Yu Gao, Liangyou Gu, Wenyuan Nie, Xu Zhang

“…In the molecular biological findings, quantitative real-time PCR analysis revealed similar CYP11B1 and CYP17A1 mRNA expressions in resected A/CPA specimens and in pure APA specimens. …”
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Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes by Maria Mesuraca, Olimpio Galasso, Leonardo Guido, Emanuela Chiarella, Stefania Scicchitano, Renaud Vatrinet, Giovanni Morrone, Heather M. Bond, Giorgio Gasparini

“…Interestingly, this decrease was mirrored by the similarly reduced expression of PPARγ, a known target of ZNF423 with anti-inflammatory and chondroprotective properties. The ZNF521 mRNA instead was abundant in all primary chondrocytes studied; the RNAi-mediated silencing of this gene significantly altered the COL2A/COL1 expression ratio, associated with the maintenance of the differentiated phenotype, in chondrocytes cultivated in alginate beads. …”
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Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease by Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana

“…Trigeminal ganglions (TG) were collected to determine SP levels by ELISA and transient receptor potential cation channel subfamily V member 1 (TRPV1), transient receptor potential cation channel subfamily M (melastatin) member 8, c-Fos, and activating transcription factor 3 (ATF3) mRNA levels by real-time polymerase chain reaction. …”
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Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds by Zhuo Deng, Jing Wang, Wentao Lyu, Xuwen Wieneke, Robert Matts, Xi Ma, Guolong Zhang

“…Eight compounds were subsequently observed to be comparable to sodium butyrate in inducing PBD3 mRNA expression in parental IPEC-J2 cells in the low micromolar range. …”
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Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sou... by Zhili Ding, Na Luo, Youqin Kong, Jingfen Li, Yixiang Zhang, Fang Cao, Jinyun Ye

“…The MnSR-BI primary structure consisted of a CD36 domain that contained two transmembrane regions at the N- and C-terminals of the protein. SR-BI mRNA expression was specifically detected in muscle, gill, ovum, intestine, hepatopancreas, stomach, and ovary tissues. …”
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Comprehensive multi-omics analysis showed that CDC6 is a potential prognostic and immunotherapy biomarker for multiple cancer types including HCC by Chenxuan Li, En-di Zhang, Rui Yu, Bo Yuan, Yunxin Yang, Zhong Zeng, Hanfei Huang

“…Methods: Explored CDC6 mRNA and protein expression in normal human tissues and tumors using TCGA, GTEx, and HPA. …”
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Oral Supplementation with Three Vegetable Oils Differing in Fatty Acid Composition Alleviates High-Fat Diet-Induced Obesity in Mice by Regulating Inflammation and Lipid Metabolism by Waleed Aldamarany, Huang Taocui, Deng Liling, Yang Wanfu, Geng Zhong

“…It also decreased leptin, pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and (IL)-1beta (IL-1β), and increased anti-inflammatory adipokine adiponectin at both secretion and mRNA expression levels in the epididymal adipose tissue (EAT). …”
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Epigenome-wide association study of Chinese monozygotic twins identifies DNA methylation loci associated with estimated glomerular filtration rate by Xueting Qi, Jingjing Wang, Tong Wang, Weijing Wang, Dongfeng Zhang

“…Gene expression profiling sequencing was employed to measure mRNA expression levels, and the GEE model was used to investigate the association between gene expression and eGFR. …”
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Identification of Immune-Related lncRNA Pairs and Construction and Validation of a New Prognostic Signature of Colon Cancer by Minxian Xu, Qing Li, Jianfang Zhang, Hui Xie

“…We downloaded the expression matrix of mRNA and lncRNA of patients with colon cancer and their clinical information from the public TCGA database. …”
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FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent man... by Xinghui Li, Qi Liang, Lu Liu, Shujun Chen, Yong Li, Yu Pu

“…Methylated RNA immunoprecipitation and mRNA stability assays were performed to elucidate the mechanism of FTO-mediated N6-methyladenosine (m6A) modification. …”
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Paradoxical Glucose-Sensitizing yet Proinflammatory Effects of Acute ASP Administration in Mice by Alexandre Fisette, Pegah Poursharifi, Katerina Oikonomopoulou, Mercedes N. Munkonda, Marc Lapointe, Katherine Cianflone

“…ASP supplementation induced a proinflammatory phenotype, with higher levels of cytokines including IL-6 and TNF-α in plasma and in adipose tissue, liver, and skeletal muscle mRNA. Additionally, ASP increased M1 macrophage content of these tissues. …”
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Vitamin D Regulates the Expression of Glucocorticoid Receptors in Blood of Severe Asthmatic Patients by Bassam Mahboub, Saba Al Heialy, Mahmood Yaseen Hachim, Rakhee K. Ramakrishnan, Ashraf Alzaabi, Rania Medhat Seliem, Laila Ibraheem Salameh, Sameen Masooma Toor, Fathelrahman Salem Shendi, Ola Mohamed Al Ali, Basil Khalid Safarini, Wafa Taleb Erabia, Rabih Halwani, Qutayba Hamid

“…Upon VitD supplementation of severe asthmatic patients, there was a significant increase in the blood levels of GR-α with no change in GR-β mRNA expression. VitD supplementation also suppressed the blood levels of IL-17F and IL-4. …”
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Regulatory Effect of Iguratimod on the Balance of Th Subsets and Inhibition of Inflammatory Cytokines in Patients with Rheumatoid Arthritis by Yunzhi Xu, Qi Zhu, Jinglve Song, Hongli Liu, Yutong Miao, Fan Yang, Feiyan Wang, Wenjing Cheng, Yebin Xi, Xiaoyin Niu, Dongyi He, Guangjie Chen

“…Frequencies of Th1, Th17, Tfh, and Treg were quantified using flow cytometry, and serum cytokines were detected by enzyme-linked immunosorbent assay. mRNA expression of cytokines and transcriptional factor were quantified by RT-PCR. …”
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New insights into the mechanism of triphenyl phosphate and its metabolite diphenyl phosphate in diabetic kidney disease by Ting Fang, Qiaoyan Liu, Xinxin Huangfu, Hongkai Zhu, Hongwen Sun, Liming Chen

“…The study also detected mRNA expression of core targets molecularly docked with TPHP and DPHP using real-time-PCR. …”
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Exacerbation of diabetes due to F. Nucleatum LPS-induced SGLT2 overexpression in the renal proximal tubular epithelial cells by Aiko Seki, Koichiro Kajiwara, Jumpei Teramachi, Masahiko Egusa, Takuya Miyawaki, Yoshihiko Sawa

“…The renal tissue SGLT2 mRNA amounts and the number of renal proximal tubules with overexpressed SGLT2 in the lumen were more in the Fn-LPS-administered-diabetic mice than in diabetic mice. …”
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