FOSL2 activates TGF‐β1‐mediated GLUT1/mTOR signaling to promote diabetic kidney disease by Xuelin He, Min Xia, Guanghui Ying, Qien He, Zhaogui Chen, Li Liu, Qiao Zhang, Jianxin Cai

“…Knockdown of FOSL2 reduced the mRNA expression of TGF‐β1 to decrease the protein expression of GLUT1 and mTOR in the kidney tissues of DKD mice, and TGF‐β1 reversed the effects caused by knockdown of FOSL2. …”
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Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra by Jing Zhen, Yue Guo, Tom Villani, Steve Carr, Thomas Brendler, Davis R. Mumbengegwi, Ah-Ng Tony Kong, James E. Simon, Qingli Wu

“…Cell-based assays show that the leaf extract inhibits the mRNA expression of proinflammatory genes (IL-6, iNOS, and TNF-α) by using RT-qPCR, implying its anti-inflammatory effects. …”
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Pharmacological effects of fibroblast growth factor 21 are sex-specific in mice with the <em>lethal yellow</em> (A^y) mutation by E. N. Makarova, T. V. Yakovleva, N. Yu. Balyibina, K. O. Baranov, E. I. Denisova, A. D. Dubinina, N. A. Feofanova, N. M. Bazhan

“…FGF21 administration decreased hypothalamic POMC mRNA only in males. Thus, the pharmacological effect of FGF21 were significantly different in male and female Ay -mice; unlike males, females were resistant to catabolic effects of FGF21.…”
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The comprehensive assessment of epigenetics changes during skin development by Li Lei, Ling Jiang, Yibo Hu, Menglu Chen, Jinhua Huang, Jing Chen, Qinghai Zeng

“…However, the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization, cell-matrix adhesion, innate immune response, mitochondrial function and mRNA processing. In addition, epigenetic changes following UV exposure were more pronounced in the elderly skin compared to the younger skin. …”
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Endometrial factors in the implantation failure spectrum: protocol of a MUltidisciplinary observational cohort study in women with Repeated Implantation failure and recurrent Misca... by Servaas Morré, Ron van Golde, Andrea Romano, Linda Stevens Brentjens, Denise Habets, Janneke Den Hartog, Salwan Al-Nasiry, Lotte Wieten, Aafke Van Montfoort

“…The study parameters are the steroid profile (steroid levels and mRNA levels, protein expression and activity of steroid enzymes) in endometrial tissue and peripheral blood, as well as the activating or inhibitory phenotype of uNK cells based on receptor expression in menstrual blood and endometrial tissue and determination of the vaginal and endometrial microbiome using the inter spacer bacterial profiling technique.Ethics and dissemination The protocol is approved by the local medical ethical review committee at the Maastricht University Medical Centre. …”
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Anti-allergic effect of 5,7-dihydroxy-4-methylcoumarin in IgE-mediated RBL-2H3 cells and PCA murine model by Sugeun Gwon, Seong-Ah Shin, Moonsu Kim, Sun Young Moon, Seyeon Choi, Minji Kim, Jun Hyuck Lee, Hyun Ho Park, Chang Sup Lee

“…Our findings demonstrated that 5,7-dihydroxy-4-methylcoumarin reduced the release of histamine and β-hexosaminidase and downregulated the mRNA expression of allergic-inflammatory cytokines, such as interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha, as well as the inflammatory enzyme cyclooxygenase-2. …”
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Maraviroc/cisplatin combination inhibits gastric cancer tumoroid growth and improves mice survival by Bárbara Mora-Lagos, María Elena Reyes, Lorena Lobos-Gonzalez, Matías del Campo, Kurt Buchegger, Louise Zanella, Ismael Riquelme, Carmen Gloria Ili, Priscilla Brebi

“…The effects of the MVC/CDDP combination on apoptosis and cell cycle progression were correlated with the increase in CDDP (dose-dependent). The mRNA levels of C-C Motif Chemokine Ligand 5 (CCL5), the main ligand for CCR5, decreased significantly in cells treated with the MVC/CDDP combination. …”
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The Role of Cholesterol during the Ovarian Maturation and Lipid Metabolism of Female Chinese Mitten Crab (Eriocheir sinensis) by Huixing Guo, Haokun Hua, Jianfeng Wang, Wei Qiang, Xiaoe Xiang, Wenbin Liu, Guangzhen Jiang

“…Through the analysis of mRNA expression level of genes related to lipid metabolism, it was found that cholesterol enhanced the transcription level of genes related to lipid synthesis and transport in hepatopancreas, thereby promoting the accumulation of lipid in the organism. …”
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lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis by YiFan Wang, GuanYin Zhu, Fang Pei, ZhiHe Zhao

“…The expressions of SNHG15, miR-381-3p, and never in mitosis-related kinase 2 (NEK2) mRNA were detected by real-time quantitative polymerase chain reaction (RT-qPCR); Western blot detected NEK2 and osteoclast markers (Cathepsin K, CTSK), matrix metalloproteinase 9 (MMP9), nuclear factor of activated T cell 2 (NFAT2), and tartrate-resistant acid phosphatase (TRAP) protein levels; cell proliferation was detected by Cell Counting Kit-8 (CCK-8), and the formation of osteoclasts was observed by TRAP staining; the F-actin skeleton was stained with tetramethylrhodamine isothiocyanate (TRITC) phalloidin; cell migration rate was detected by Transwell; dual-luciferase reporter gene assay and RNA-binding protein immunoprecipitation (RIP) assay verified the targeting relationship between miR-381-3p, SNHG15, and NEK2. …”
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(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK by Shinji Takai, Rie Matsushima-Nishiwaki, Seiji Adachi, Hideo Natsume, Chiho Minamitani, Jun Mizutani, Takanobu Otsuka, Haruhiko Tokuda, Osamu Kozawa

“…EGCG significantly reduced the IL-6 synthesis and IL-6 mRNA expression stimulated by PDGF-BB, EGCG reduced the PDGF-BB-stimulated IL-6 synthesis also in primary-cultured osteoblasts. …”
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Anti-SARS-Cov-2 S-RBD IgG Formed after BNT162b2 Vaccination Can Bind C1q and Activate Complement by Anas H. A. Abu-Humaidan, Fatima M. Ahmad, Dima Awajan, Raba’a F. Jarrar, Nader Alaridah

“…In this study, we investigated C1q binding and subsequent complement activation by anti-spike (S) protein receptor-binding domain (RBD) specific antibodies produced following vaccination with either the mRNA vaccine BNT162b2 or the inactivated vaccine BBIBP-CorV. …”
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The key role of the NUDT3 gene in lung adenocarcinoma progression and its association with the manganese ion metabolism family by Deyong Ge, Xinyu Xu, Liyi Fang, Huihui Tao

“…Quantitative PCR (qPCR) was conducted to evaluate the relative mRNA expression of NUDT3 in A549 lung adenocarcinoma cell lines compared to BEAS-2B normal bronchial epithelial cell lines. …”
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Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study by Yun Mi Lee, Kyuhyung Jo, So Yeon Kim, Chang-Seob Seo, Eunjung Son, Aejin Kim, Dong-Seon Kim

“…YWS showed gastroprotective effects through histamine downregulation, while prostaglandin E2 (PGE2) and mucin were upregulated. The mRNA levels of H2R, M3R, CCK2R, and H⁺/K⁺ ATPase were significantly decreased following treatment with YWS. …”
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IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response by Jun-feng Zhu, Ying Xu, Jian Zhao, Xue Li, Xinrui Meng, Tian-qi Wang, Ben-yao Zou, Peng-yan Zhao, Qi Liu, Chang-long Lu, Fang-liang Zheng, Hong-sheng Liu

“…The T and B cell subsets were measured by flow cytometry. The expression of mRNA of transcription factors was measured by quantitative real-time PCR. …”
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FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids by Simone Isling Pærregaard, Marianne Agerholm, Annette Karen Serup, Tao Ma, Bente Kiens, Lise Madsen, Karsten Kristiansen, Benjamin Anderschou Holbech Jensen

“…Moreover, ω3-PUFA fed mice had increased circulating protein levels of the anti-inflammatory adipokine, adiponectin, decreased fasting insulin levels, and decreased mRNA expression of several proinflammatory molecules within visceral adipose tissue. …”
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2′-O-Methylperlatolic Acid Enhances Insulin-Regulated Blood Glucose-Lowering Effect through Insulin Receptor Signaling Pathway by Wang Yinghao, Guan Qiaoli, Liu Guanfu, Wu Xiaoyun, Wang Xuanjun, Sheng Jun

“…Next, qRT-PCR was performed to detect the mRNA expression of genes involved in lipid and glucose metabolism in the liver and muscle tissues. …”
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FLT3 is associated with dendritic cell infiltration, tertiary lymphoid structure construction, and predict response to checkpoint inhibitors immunotherapy in solid cancers by Yongchang Tang, Hong Wang, Jiankun Zhang, Chunhui Yang, Fei Xu, Yan Song, Tianen Li, Qiangbo Zhang

“…We analyzed the frequency of FLT3 alterations, its mRNA expression levels, and its prognostic implications across multiple cancer types. …”
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Ribosomal proteins mediate non-canonical regulation of gut inflammatory signature by crop contaminant deoxynivalenol by Juil Kim, BoGyoung Song, Ki-Hyung Kim, Yuseok Moon

“…Through a combination of transcriptomic analysis and experimental models, we demonstrate that while RPL13A negatively regulates inflammation by enhancing the anti-inflammatory transcription factor ATF3, RPS3 acts as a proinflammatory driver, promoting chemokine production via activation of MAPK pathways, transcriptional upregulation of EGR-1, and stabilization of mRNA through cytosolic translocation of HuR. Our findings reveal a dynamic interplay between RPL13A and RPS3, wherein RPL13A counteracts the proinflammatory effects of RPS3, offering a finely tuned regulatory axis in the inflammatory response to environmental toxins. …”
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Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M... by Yuanping Li, Dan Zheng, Dayue Shen, Xilan Zhang, Xiaoyun Zhao, Hui Liao

“…Results. (1) Compared to the HG group, the Ka and HSYA 100 μM groups decreased iNOS and TNF-α levels and increased Arg-1 and CD206 expressions significantly (protein and mRNA: p<0.05, respectively). (2) The podocyte survival rate of Ka 8 μM was higher than that of HG, and the rates of (Ka) MS and (HSYA 100 μM) MS were higher than that of (HG) MS significantly (all: p<0.05). (3) TNF-α and IL-1β levels of Ka and HSYA 100 μM were significantly lower than those of the HG group, and both levels in the (Ka) MS and (HSYA) MS were lower than those in the (HG) MS group significantly (p<0.05, respectively). …”
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Identification of a novel immunogenic cell death-related classifier to predict prognosis and optimize precision treatment in hepatocellular carcinoma by Dongjing Zhang, Bingyun Lu, Qianqian Ma, Wen Xu, Qi Zhang, Zhiqi Xiao, Yuanheng Li, Ren Chen, An-jiang Wang

“…In this study, we systematically analyzed the mRNA profiles of ICD-related genes in 1847 HCC patients and identified three molecular subtypes with significantly different immune features and prognostic stratification. …”
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