Blockade of ICAM-1 Improves the Outcome of Polymicrobial Sepsis via Modulating Neutrophil Migration and Reversing Immunosuppression by Yan-jun Zhao, Wen-jing Yi, Xiao-jian Wan, Jun Wang, Tian-zhu Tao, Jin-bao Li, Jia-feng Wang, Xiao-ming Deng

“…The results demonstrated that ICAM-1 mRNA was upregulated in lung, thymus, and spleen of CLP mice. …”
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Advancements in the Treatment of Mucopolysaccharidoses: From Established Therapies to Gene Therapy by Joanna Wanat, Wojciech Homa, Aleksandra Warunek, Gabriela Gronowicz, Izabela Dzikowska, Agata Siejka, Daria Stefaniak, Weronika Zielińska, Michał Chról

“…Among the promising new treatments are: blood-brain barrier (BBB)-penetrable enzyme replacement therapy (ERT), substrate reduction therapy, nonsense suppression therapy, and mRNA engineering. However, the most advanced treatments, that are currently under investigation are in-vivo and ex-vivo gene therapy. …”
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A Novel Liposome Formulation Carrying Both an Insulin Peptide and a Ligand for Invariant Natural Killer T Cells Induces Accumulation of Regulatory T Cells to Islets in Nonobese Dia... by Hidetoshi Akimoto, Emi Fukuda-Kawaguchi, Omar Duramad, Yasuyuki Ishii, Kazunari Tanabe

“…Cotransfer of diabetogenic T cells and splenocytes of NOD mice treated with RGI-3100-iB, but not liposomal α-GalCer encapsulating an unrelated peptide, to NOD-SCID mice resulted in the prevention of diabetes and elevation of Foxp3 mRNA expression in the islets. These data indicate that the migration of insulin B-peptide-specific Tregs to islet of NOD mice that are involved in the suppression of pathogenic T cells related to diabetes onset and progression could be enhanced by the administration of liposomes containing α-GalCer and insulin B peptide.…”
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A hypomorphic mutation in the mouse Csn1s1 gene generated by CRISPR/Cas9 pronuclear microinjection by A. V. Smirnov, T. A. Shnaider, A. N. Korablev, A. M. Yunusova, I. A. Serova, N. R. Battulin

“…We performed pronuclear microinjections with Cas9 mRNA and sgRNA against the first coding exon of the mouse Csn1s1 gene to introduce random mutations in the α-casein (Csn1s1) signal peptide sequence at the beginning of the mouse gene. …”
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The Influence of Monoamine Oxidase Variants on the Risk of Betel Quid-Associated Oral and Pharyngeal Cancer by Ping-Ho Chen, Bin Huang, Tien-Yu Shieh, Yan-Hsiung Wang, Yuk-Kwan Chen, Ju-Hui Wu, Jhen-Hao Huang, Chun-Chia Chen, Ka-Wo Lee

“…A significant trend of MAO-A mRNA expression was found in in vitro studies. Using paired human tissues, we confirmed the significantly decreased expression of MAO-A and MAO-B in cancerous tissues when compared with adjacent noncancerous tissues. …”
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Biochanin a modulates steroidogenesis and cellular metabolism in human granulosa cells through TAS2Rs activation: a spotlight on ovarian function by Francesca Paola Luongo, Sofia Passaponti, Alesandro Haxhiu, Irene Ortega Baño, Rosetta Ponchia, Giuseppe Morgante, Paola Piomboni, Alice Luddi

“…Results BCA treatment significantly upregulated TAS2R14 and TAS2R43 expression, leading to a 70% increase in StAR mRNA levels and a twofold increase in CYP17A1 expression (p < 0.05). …”
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Dopamine receptors and key elements of the neurotrophins (BDNF, CDNF) expression patterns during critical periods of ontogenesis in the brain structures of mice with autism-like be... by P. D. Pravikova, M. A. Arssan, E. A. Zalivina, E. M. Kondaurova, E. A. Kulikova, I. I. Belokopytova, V. S. Naumenko

“…A decrease in the expression of Cdnf, Bdnf and its receptor Ntrkb2, as well as dopamine receptors (Drd1, Drd2) was detected in BTBR mice in the postnatal period of ontogenesis mainly in the frontal cortex, while in the hippocampus of mature mice (p60), only a decrease in the Drd2 mRNA level was revealed. The obtained results suggest that the decrease in the expression levels of CDNF, BDNF-TrkB and dopamine receptors in the frontal cortex in the postnatal period can lead to significant changes in both the morphology of neurons and dopamine neurotransmission in cortical brain structures. …”
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Kojic Acid Derivative as an Antimitotic Agent That Selectively Kills Tumour Cells by Giuseppina Pichiri, Marco Piludu, Terenzio Congiu, Nicole Grandi, Pierpaolo Coni, Monica Piras, Mariusz Jaremko, Joanna Izabela Lachowicz

“…Light and electron microscopy observations were supported by the next generation sequencing (NGS), cytoflow, and spectroscopy analysis of mRNA and biomolecules, respectively. <b>Results:</b> The light and electron microscopy observations showed that L1 treatment leads to significant morphological changes in Caco2 cells, which are characteristic of mitosis arrest. …”
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Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…This approach utilizes an engineered small RNA (sRNA) to inhibit translation of the mRNA for a target gene and produced inducible and reversible protein knockdown. …”
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Exosomal miR-21-5p from glioma associated mesenchymal stem cells promotes the progression and glycolysis of glioblastoma via PDHA1 by Yanbin Zhang, Peng Lv, Qing Zhang, Wei Xiang, Xiaobing Jiang, Zijun Guo, Tao Zhang

“…Mechanismly, miR-21-5p directly binds to the mRNA of PDHA1 and downregulates its transcription, thereby promoting GBM glycolysis. …”
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Role of Sex Hormones in the Development and Progression of Hepatitis B Virus-Associated Hepatocellular Carcinoma by Maurizio Montella, Giovanni D’Arena, Anna Crispo, Mario Capunzo, Flavia Nocerino, Maria Grimaldi, Antonio Barbieri, Anna Maria D’Ursi, Mario Felice Tecce, Alfonso Amore, Massimiliano Galdiero, Gennaro Ciliberto, Aldo Giudice

“…Additionally, sex hormones can also affect HBV-related hepatocarcinogenesis by inducing epigenetic changes such as the regulation of mRNA levels by microRNAs (miRNAs), DNA methylation, and histone modification in liver tissue. …”
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Interleukin-10 Polymorphisms in Association with Prognosis in Patients with B-Cell Lymphoma Treated by R-CHOP by Min Kyeong Kim, Kyong-Ah Yoon, Eun Young Park, Jungnam Joo, Eun Young Lee, Hyeon-Seok Eom, Sun-Young Kong

“…Previous studies have revealed that the transcription of IL10 mRNA and its protein expression may be infl uenced by several single-nucleotide polymorphisms in the promoter and intron regions, including rs1800896, rs1800871, and rs1800872. …”
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Promising markers of CIMP+ colon tumors identified on the basis of TCGA data analysis by G. S. Krasnov, A. D. Beniaminov, R. A. Tychko, G. A. Puzanov, R. O. Novakovskiy, A. V. Kudryavtseva, A. A. Dmitriev

“…To verify the obtained data in independent sampling, first, the quantitative PCR was used to assess the relative mRNA level of the AMOTL1, ZNF43, ZNF134, and CHFR genes in 30 paired (tumor/histologically normal tissue) colon samples. …”
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Cell-free osteoarthritis treatment with dual-engineered chondrocyte-targeted extracellular vesicles derived from mechanical loading primed mesenchymal stem cells by Peng Wang, Haiyue Zhao, Wei Chen, Yuhui Guo, Shuo Zhang, Xin Xing, Shuai Yang, Fengkun Wang, Juan Wang, Zengwu Shao, Yingze Zhang

“…Subsequent miRNA sequencing reveals that ML-EVs exert their effects by delivering miR-27b-3p, which targets ROR1 mRNA in chondrocytes and suppresses downstream NF-κB pathways. …”
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Myocardial Expression of PPARγ and Exercise Capacity in Patients after Coronary Artery Bypass Surgery by Izabela Wojtkowska, Tomasz A. Bonda, Jadwiga Wolszakiewicz, Jerzy Osak, Andrzej Tysarowski, Katarzyna Seliga, Janusz A. Siedlecki, Maria M. Winnicka, Ryszard Piotrowicz, Janina Stępińska

“…The mean distance 1 month after CABG in HF was 397±85 m versus 420±93 m in non-HF. PPARγ mRNA expression was similar in both HF and non-HF groups. 6MWT distance 1 month after CABG was inversely correlated with PPARγ level only in HF group. …”
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Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism, and hormone levels by Nathalie Idlin, Sivakumar Krishnamoorthy, Magdalena Wolczyk, Mouad Fakhri, Michal Lechowski, Natalia Stec, Jacek Milek, Pratik Kumar Mandal, Jaroslaw Cendrowski, Christos Spanos, Magdalena Dziembowska, Katarzyna Mleczko-Sanecka, Juri Rappsilber, Gracjan Michlewski

“…Abstract Background HuR/ELAV1, a ubiquitous RNA-binding protein, belongs to the RNA-binding protein family and is crucial for stabilizing and regulating the translation of various mRNA targets, influencing gene expression. Elevated HuR levels are associated with multiple disorders, including cancer and neurodegenerative diseases. …”
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CD154 Induces Interleukin-6 Secretion by Kidney Tubular Epithelial Cells under Hypoxic Conditions: Inhibition by Chloroquine by Antoine Dewitte, Julien Villeneuve, Sébastien Lepreux, Marion Bouchecareilh, Xavier Gauthereau, Claire Rigothier, Christian Combe, Alexandre Ouattara, Jean Ripoche

“…Both intracellular IL-6 expression and secretion were stimulated by CD154 and associated with a strong upregulation of IL-6 mRNA and increased transcription. Searching for inhibitors of CD154-mediated IL-6 production by HK-2 cells in hypoxic conditions, we observed that chloroquine, a drug that has been repurposed as an anti-inflammatory agent, alleviated this induction. …”
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CircRNAs in colorectal cancer: potential roles, clinical applications, and natural product-based regulation by Jingjing Yang, Jingjing Yang, Qiqi Fan, Yiyun Wang, Yuanyue Liu, Xiaoning Xu, Yeqi Liang, Jiakang Xie, Jiajie Li, Fengting Ai, Yong Cao, Shangzhen Yu, Jinman Liu

“…Additionally, natural products such as flavonoids and glycosides, by modulating circRNA-miRNA-mRNA networks, offer promising therapeutic strategies. …”
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Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines by Jovanović Krivokuća Milica, Legner Janko, Ćujić Danica, Vilotić Aleksandra, Grubiša Ivana, Vićovac Ljiljana, Bojić-Trbojević Žanka

“…Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. …”
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A nociceptor-specific RNAi screen in Drosophila larvae identifies RNA-binding proteins that regulate thermal nociception by Amber Dyson, Gita Gajjar, Katherine C. Hoffman, Dakota Lewis, Sara Palega, Erik Rangel Silva, James Auwn, Andrew Bellemer

“…Other screen isolates have previously described roles in regulating nociceptor morphology and mRNA processing, but less clear roles in regulating nociceptor function. …”
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