Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model by Samah Fouad, Awad Rizk, Esam Mosbah, Mostafa M. Nabeeh, Walaa Awadin, Ayman S. Elmezayyen, Ekramy Elmorsy, Adel Zaghloul

“…After a 30-days follow-up, macroscopic examination, histological studies, and mRNA evaluation for TGFβ-1and IL-6, were conducted. …”
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NT5E (CD73) as a prognostic biomarker and therapeutic target associated with immune infiltration in lung adenocarcinoma by Leyan Chen, Tuoya Qi, Bishu Zhang, Xuelong Wang, Mingfeng Zheng

“…We found that NT5E was significantly overexpressed at both the mRNA and protein levels in LUAD tissues. Elevated NT5E expression was significantly linked to multiple clinicopathological factors, including metastasis and pathological stage, and served as a strong predictor of poor prognosis in LUAD patients. …”
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Characterization of Vaccine-Enhanced Humoral Immune Responses Against Emergent SARS-CoV-2 Variants in a Convalescent Cohort by Jared Sheehan, Amber J. Trauth, Michael E. Hagensee, Alistair J. Ramsay

“…Our primary goal was to characterize hybrid antibody (Ab) responses in a patient cohort infected with ancestral Wuhan-Hu-1 virus and vaccinated between 6 and 10 months later with the Wuhan-Hu-1–based BNT162b2 mRNA vaccine. We were particularly interested in determining the efficacy of neutralizing Ab responses against subsequently emergent SARS-CoV-2 variants. …”
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circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD by Xiao-Lin Fu, Fu-Tao He, Mo-Han Li, Chun-Yan Fu, Jian-Zhi Chen

“…Actinomycin D-treated HRMECs were used to confirm the mRNA stability of phosphoinositide-3 kinase catalytic subunit δ (PIK3CD). …”
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A Novel Pathogenic Splicing Mutation of OFD1 is Responsible for a Boy with Joubert Syndrome Exhibiting Orofaciodigital Spectrum Anomalies, Polydactyly and Retinitis Pigmentosa by Chen L, Zhao MF, Deng HW, Liao M, Fan LL, Zhong QB, Wang J, Li K, Wu ZH, Yin JY

“…Whole exome sequencing and Sanger sequencing revealed a splicing mutation (NM_003611.2, c.2387+1G>A) in the OFD1 gene of the patient and his mother. mRNA sequencing further confirmed this mutation. …”
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Ameliorative Effect of Hovenia dulcis Fruit Pedicel Polysaccharides on Alcohol-Induced Intestinal Injury in Mice Investigated Using 16S rRNA Sequencing and Metabolomics by ZHANG Yuchao, ZHU Sijie, LIU Liangyu, WANG Jianyun, ZHANG Jiaxin, SU Zexiong, CHEN Jiangyan, LIU Xudong

“…Furthermore, HDPs inhibited the mRNA and protein expression of apical sodium-dependent bile acid transporter (ASBT) in the intestine of alcohol-exposed mice, thus reducing the absorption of bile acids and alleviating the negative impact of alcohol on the intestine. …”
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The Expression and Clinical Significance of Different Forms of Mer Receptor Tyrosine Kinase in Systemic Lupus Erythematosus by Huaqun Zhu, Xiaolin Sun, Lei Zhu, Fanlei Hu, Lianjie Shi, Zhanguo Li, Yin Su

“…Real-time quantitative PCR was applied to evaluate the mRNA levels of MerTK and ADAM17. Results. Both mMer expression on CD14+ monocytes/macrophages and sMer levels in plasma significantly increased in SLE patients compared to healthy subjects. …”
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Bivalirudin exerts antiviral activity against respiratory syncytial virus-induced lung infections in neonatal mice by Zhuang Shihao, Tang Qiuyu, Chen Ping, Wang Chengyi, Liu Guanghua

“…Furthermore, RSV-infected mice demonstrated altered clotting parameters such as D-dimer, soluble thrombomodulin, and increased inflammatory cytokines IL-5, 6, IFN-γ, IL-13, and CXCL1. Additionally, the mRNA expression analysis displayed increased levels of IL-33, TLR3, and TLR7 genes in RSV-infected lung tissue. …”
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miR-149 Alleviates Ox-LDL-Induced Endothelial Cell Injury by Promoting Autophagy through Akt/mTOR Pathway by Zhongsheng Zhu, Jinyu Li, Rui Tong, Xiaorong Zhang, Bo Yu

“…RT-qPCR and western blot were carried out to determine the mRNA and protein expressions of Akt and mTOR. Results. …”
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Antiviral Drugs and Vaccines for Omicron Variant: A Focused Review by Nidhi Garg, Ananya Sree Kunamneni, Pankaj Garg, Sandeep Sharma, Divakar Sharma, Adinarayana Kunamneni

“…Regarding vaccine efficacy (VE), the 3-dose VE with mRNA vaccines at 14–60 days was found to be 71.6%, and after 60 days, it is 47.4%. …”
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Dietary Cocoa Powder Improves Hyperlipidemia and Reduces Atherosclerosis in apoE Deficient Mice through the Inhibition of Hepatic Endoplasmic Reticulum Stress by Hua Guan, Yan Lin, Liang Bai, Yingfeng An, Jianan Shang, Zhao Wang, Sihai Zhao, Jianglin Fan, Enqi Liu

“…The groups fed dietary cocoa powder showed a significant reduction in both plasma cholesterol levels and aortic atherosclerosis compared to the control group. Analysis of mRNA profiling of aortic atherosclerotic lesions revealed that the expression of several genes related to apoptosis, lipid metabolism, and inflammation was significantly reduced, while the antiapoptotic gene Bcl2 was significantly increased in the cocoa powder group compared to the control. …”
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Downregulation of VRK1 Inhibits Progression of Lung Squamous Cell Carcinoma through DNA Damage by Ning Du, Boxiang Zhang, Yunfeng Zhang

“…The expression of VRK1 mRNA was detected in 25 LUSC clinical tissue samples by RT-PCR. …”
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Crop protection by RNA interference: a review of recent approaches, current state of developments and use as of 2013 by Kirsten Germing, Cecilia Andrea Díaz Navarrete, Andreas Schiermeyer, Udo Hommen, Luise Zühl, Sebastian Eilebrecht, Elke Eilebrecht

“…Abstract RNAi-based applications aim to inhibit the expression of specific essential genes in target organisms by uptake and processing of double-stranded RNA and subsequent degradation of target gene mRNA. While the research on RNAi-based pesticides was initially limited to endogenous applications (i.e., production of the dsRNA by the crop), the development of technologies for exogenous applications like RNAi sprays in particular has increased in recent years. …”
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Salvia-Nelumbinis Naturalis Formula Improved Inflammation in LPS Stressed Macrophages via Upregulating MicroRNA-152 by Zansong Ma, Xiangbing Shu, Jie Huang, Haiyan Zhang, Zhen Xiao, Li Zhang

“…Kupffer cells were isolated from C57BL/6 mice and treated with LPS or LPS and SNN-containing serum; the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was assessed. …”
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Paeonol Attenuates Methotrexate-Induced Cardiac Toxicity in Rats by Inhibiting Oxidative Stress and Suppressing TLR4-Induced NF-κB Inflammatory Pathway by Abdulla Y. Al-Taher, Mohamed A. Morsy, Rehab A. Rifaai, Nagwa M. Zenhom, Seham A. Abdel-Gaber

“…MTX-induced inflammatory effects are evidenced by the increased cardiac toll-like receptor 4 (TLR4) mRNA expression and protein level as well as increased cardiac tumor necrosis factor- (TNF-) α and interleukin- (IL-) 6 levels along with increased nuclear factor- (NF-) κB/p65 immunostaining. …”
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Left ventricle function and post-transcriptional events with exercise training in pigs. by Stephanie L Samani, Shayne C Barlow, Lisa A Freeburg, Traci L Jones, Marlee Poole, Mark A Sarzynski, Michael R Zile, Tarek Shazly, Francis G Spinale

“…Additionally, miR-22 and miR-30e decreased with exercise and mapped to a localized inflammatory process, the inflammasome (NLRP-3, whereby a 2-fold decrease in NLRP-3 mRNA occurred with exercise (p < 0.05).<h4>Conclusion</h4>Chronic exercise reduced LV chamber and myocardial stiffness and was correlated to miRs that map to myocardial relaxation processes as well as local inflammatory pathways. …”
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Association of TNF-α with Impaired Migration Capacity of Mesenchymal Stem Cells in Patients with Systemic Lupus Erythematosus by Linyu Geng, Xia Li, Xuebing Feng, Jiyun Zhang, Dandan Wang, Jinyun Chen, Rui Liu, Haifeng Chen, Lingyun Sun

“…Increased tumor necrosis factor α (TNF-α) in SLE serum significantly inhibited the migration capacity and in vivo homing capacity of SLE BMSCs via a specific TNF receptor I (TNFRI) manner, in which decreased HGF mRNA production caused by the activation of I kappa B kinase beta (IKK-β) pathway is partially involved. …”
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Promiscuous Chemokine Antagonist (BKT130) Suppresses Laser-Induced Choroidal Neovascularization by Inhibition of Monocyte Recruitment by Shira Hagbi-Levi, Michal Abraham, Liran Tiosano, Batya Rinsky, Michelle Grunin, Orly Eizenberg, Amnon Peled, Itay Chowers

“…Quantification of CD11b+ cell in the CNV area was performed, and mRNA levels of genes implicated in CNV growth were measured in the retina and RPE-choroid. …”
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Anger Emotional Stress Influences VEGF/VEGFR2 and Its Induced PI3K/AKT/mTOR Signaling Pathway by Peng Sun, Sheng Wei, Xia Wei, Jieqiong Wang, Yuanyuan Zhang, Mingqi Qiao, Jibiao Wu

“…Anger emotional stress led to abnormal expression of VEGF/VEGFR2 mRNA and protein and disorderly expression of key factors in the PI3K/AKT/mTOR signal pathway. …”
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Exposure of Mesenchymal Stem Cells to an Alzheimer’s Disease Environment Enhances Therapeutic Effects by Sang Eon Park, Hyeong Seop Kim, Soo Jin Kwon, Min-Jeong Kim, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu, Hong Bae Jeon, Duk L. Na, Jong Wook Chang

“…We demonstrated that mouse groups treated with naïve MSCs and primed MSCs showed significant reductions in cell death, ubiquitin conjugate levels, and Aβ levels, but the effects were greater in primed MSCs. Also, mRNA sequencing data analysis indicated that high levels of TGF-β induced primed-MSCs. …”
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