A Benzochalcone Derivative, (E)-1-(2-hydroxy-6-methoxyphenyl)-3-(naphthalen-2-yl)prop-2-en-1-one (DK-512), Inhibits Tumor Invasion through Inhibition of the TNFα-Induced NF-κB/MMP-... by Soon Young Shin, Chang Gun Kim, Seunghyun Ahn, You Jung Jung, Dongsoo Koh, Young Han Lee, Yoongho Lim

“…DK-512 significantly reduced tumor necrosis factor-α- (TNFα-) induced MMP-9 mRNA expression through the inhibition of RelA nuclear factor- (NF-) κB transcription factor. …”
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Identification and validation of hub gene TNFAIP6 of fibroblast-like synoviocytes in rheumatoid arthritis by LEI Lei, LYU Yuxin, ZHANG Jing

“…RT-qPCR and Western blot assay demonstrated that TNFAIP6 was significantly highly expressed at mRNA and protein levels in MH7A cells stimulated with 10 ng/mL TNF-α when compared to the cells treated with PBS (P<0.05). …”
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Circular RNA circFGFR1 Functions as an Oncogene in Glioblastoma Cells through Sponging to hsa-miR-224-5p by Qian Zhang, Shan Chen, Yingwei Zhen, Peng Gao, Zhenyu Zhang, Hao Guo, Yong Wang

“…Then, RIP, RNA pull-down, and luciferase reporter assay results showed that hsa-miR-224-5p directly binds to circFGFR1 and CXCR4 mRNA. The CXCR4 3′-untranslated region (UTR) activated luciferase activity was reduced with hsa-miR-224-5p transfection, while it is reversed when cotransfected with circFGFR1, indicating that circFGFR1 acts as a hsa-miR-244-5p sponge to increase CXCR4 expression. …”
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Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice by Hao Liu, Yiwen Li, Yujiao Zhu, Lei Ma, Haibo Xue

“…Correlation analyses revealed that the Th17 cell percentages were positively correlated with the serum TgAb titers, Notch pathway-related mRNA expression levels, and IL-17A concentrations in EAT mice (all P<0.05). …”
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MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis by Kai Yue, Ting Zhang, Huanhuan Wang, Bo Wang, Yalin Mu, Hui Li

“…Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. …”
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Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular Carcinoma by Gorkem Odabas, Metin Cetin, Serdar Turhal, Huseyin Baloglu, A. Emre Sayan, Tamer Yagci

“…Our observations in cancer databases that PLXNC1 is upregulated in HCC led us to investigate the expression profile of Plexin C1 mRNA and protein in HCC cell lines and tissues. Methods. …”
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Intraspinal Grafting of Serotonergic Neurons Modifies Expression of Genes Important for Functional Recovery in Paraplegic Rats by Krzysztof Miazga, Hanna Fabczak, Ewa Joachimiak, Małgorzata Zawadzka, Łucja Krzemień-Ojak, Marek Bekisz, Anna Bejrowska, Larry M. Jordan, Urszula Sławińska

“…Here, we analyzed the mRNA of 5-HT2A and 5-HT7 receptors (encoded by Htr2a and Htr7 genes, resp.) in motoneurons innervating tibialis anterior (TA) and gastrocnemius lateralis (GM) hindlimb muscles and the tail extensor caudae medialis (ECM) muscle in intact as well as spinal rats. …”
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Wogonin Inhibits Cardiac Hypertrophy by Activating Nrf-2-Mediated Antioxidant Responses by Xiaowen Shi, Bin Zhang, Zhenliang Chu, Bingjiang Han, Xueping Zhang, Ping Huang, Jibo Han

“…The mice were then sacrificed for histological assays, with mRNA and protein detection. To further explore the role of nuclear factor- (erythroid-derived 2-) like 2 (Nrf-2) in regulating the antioxidant effects of Wog in cardiac hypertrophy, siRNA analysis was conducted. …”
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DNA Methylation May be Involved in the Analgesic Effect of Hyperbaric Oxygen via Regulating FUNDC1 by Kun Liu, Hao Wu, Rui Gao, Guang Han

“…HBO downregulated DNMT3a mRNA and protein expression, but not those of DNMT1 and DNMT3b. …”
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Endoplasmic Reticulum Stress Regulates Scleral Remodeling in a Guinea Pig Model of Form-Deprivation Myopia by Chengcheng Zhu, Qingzhong Chen, Ying Yuan, Min Li, Bilian Ke

“…In vitro, TM induced ER stress in scleral fibroblasts, which was suppressed by 4-PBA. The mRNA expression of TGF-β1 and COL1A1 was upregulated after TM stimulation for 24 hours, but downregulated for 48 hours. …”
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Evaluation of Plaque Stability of Advanced Atherosclerotic Lesions in Apo E-Deficient Mice after Treatment with the Oral Factor Xa Inhibitor Rivaroxaban by Qianxing Zhou, Florian Bea, Michael Preusch, Hongjie Wang, Berend Isermann, Khurrum Shahzad, Hugo A. Katus, Erwin Blessing

“…Real time-PCR from thoracic aortas revealed that rivaroxaban (5 mg/kg/day) treatment reduced mRNA expression of inflammatory mediators, such of IL-6, TNF-α, MCP-1, and Egr-1 (P<.05). …”
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Th1/17-Biased Inflammatory Environment Associated with COPD Alters the Response of Airway Epithelial Cells to Viral and Bacterial Stimuli by Yifan Chen, Rakesh K. Kumar, Paul S. Thomas, Cristan Herbert

“…Primary AECs pretreated with Th1/17 cytokines exhibited enhanced expression of mRNA for proinflammatory cytokines in response to poly I:C. …”
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Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs) by Leila Mobasheri, Mohsen Khorashadizadeh, Hossein Safarpour, Maryam Mohammadi, Gholamreza Anani Sarab, Vahid Reza Askari

“…In addition, EEA pretreatment decreased TNF-α-induced mRNA expression of VCAM-1 and surface protein expression of ICAM-1 in the target cells. …”
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Effects of Ginkgo biloba Extract on Inflammatory Mediators (SOD, MDA, TNF-α, NF-κBp65, IL-6) in TNBS-Induced Colitis in Rats by Yan-Hong Zhou, Jie-Ping Yu, Yi-Fei Liu, Xiao-Jun Teng, Mei Ming, Peng Lv, Ping An, Shi-Quan Liu, Hong-Gang Yu

“…The results showed that EGB could significantly ameliorate macroscopic and histological damage, evidently elevate the activities of SOD and reduce the contents of MDA, inhibit the protein and mRNA expressions of TNF-α, NF-κBp65, and IL-6 in the colon tissues of experimental colitis in a dose-dependent manner compared with the model group. …”
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PPIH is a novel diagnostic biomarker associated with immune infiltration in cholangiocarcinoma by Jun Ye, Zhitao Chen, Chuan Zhang, Rui Xie, Haini Chen, Peng Ren

“…Results Our findings indicate that overexpression of PPIH mRNA in CHOL is associated with poor prognosis, with increased PPIH protein levels observed in CHOL tissues. …”
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Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells by Bernardo Bonilauri, Amanda C. Camillo-Andrade, Marlon D. M. Santos, Juliana de S. da G. Fischer, Paulo C. Carvalho, Bruno Dallagiovanna

“…We also used our previous next-generation sequencing data (RNA-seq) of the total and polysomal mRNA fractions of hASC to study posttranscriptional regulation during the initial steps of adipogenesis. …”
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Integrated analysis of dermatomyositis reveals heterogeneous immune infiltration and interstitial lung disease-associated endotype by Xinzhi Xu, Tianwen Qiu, Kexin Sun, Xue Han, Junxia Huang, Xiuyuan Wang, Jianchao Ge, Ji Yang

“…Transcriptomic data from skin samples was utilized to assess immune infiltration and investigate the correlation between protein and mRNA levels of biomarkers. Additionally, the prognostic value and clinical significance of identified biomarkers were validated through follow-up studies of DM patients and immunofluorescence analysis of skin tissues. …”
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Involvement of Neuropeptide Y within Paraventricular Nucleus in Electroacupuncture Inhibiting Sympathetic Activities in Hypertensive Rats by Qi Zhang, Yingying Tan, Xin Wen, Fangfang Li

“…The results showed that the enhanced arterial BP and sympathetic activities were effectively reduced by 30 days of EA treatment, and baroreflex sensitivity was improved in 2K1C hypertensive rats. The level of NPY mRNA and protein expression in the PVN was markedly upregulated by EA treatment in 2K1C rats. …”
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Recent advances of nanovaccines on cancer theranostics by Xiaobing Yuan, Xiaobing Yuan, Xiaobing Yuan, Tianyou Xu, Mubashir Hussain, Bin Liu, Jianwei Zhu, Jianwei Zhu

“…We believe that polymer nanoparticle-based nanovaccines have the most widespread applications currently, while liposome nanovaccines using mRNA are expected to see greater development in the future. …”
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Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation by Ji Weng, Yuning Liu, Ying Xu, Ruiqi Hu, Haolin Zhang, Xia Sheng, Gen Watanabe, Kazuyoshi Taya, Qiang Weng, Meiyu Xu

“…ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. …”
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