Repression of the stress granule protein G3BP2 inhibits immune checkpoint molecule PD‐L1 by Yanhong Zhang, Changli Yue, Anna M. Krichevsky, Igor Garkavtsev

“…Cancer cells treated with C108 or cancer cells with genetically repressed G3BP2 had decreased PD‐L1 expression due to enhanced mRNA degradation. Our study provides a compelling mechanism linking stress granule formation and immune checkpoint program of cancer, suggesting this link may provide new opportunities for improving anticancer immunotherapy.…”
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Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea by Katsuo Tomoyose, Yuka Okada, Takayoshi Sumioka, Masayasu Miyajima, Kathleen C. Flanders, Kumi Shirai, Tomoya Morii, Peter S. Reinach, Osamu Yamanaka, Shizuya Saika

“…Immunohistochemistry showed that immunoreactivity for active form of TGFβ1 and VEGF was detected in subepithelial stroma at the site of cauterization in both genotypes of mice, but the immunoreactivity seemed less marked in mice lacking TRPV1. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. …”
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Effects of Bile Acids on Growth Performance, Hepatopancreatic Antioxidant Capacity, Intestinal Immune-Related Gene Expression, and Gut Microbiota of <i>Penaeus vannamei</i> by Yun Zhao, Duanduan Chen, Hui Wang

“…Furthermore, BA supplementation increased the activity of antioxidant-related enzymes in the hepatopancreas and enhanced the mRNA expression levels of gut-associated immune genes. …”
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Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells by Po-Jung Tsai, Mei-Ling Chang, Ching-Mei Hsin, Chung-Chieh Chuang, Lu-Te Chuang, Wen-Huey Wu

“…OF and CM also suppressed the mRNA expression of interleukin- (IL-) 1β, IL-6, IL-8, tumor necrosis factor- (TNF-) α, and transforming growth factor- (TGF-) β by HepG2 cells treated with conditioned medium derived from lipopolysaccharide-treated THP-1 monocytes. …”
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Gene Expression of CD70 and CD27 Is Increased in Alopecia Areata Lesions and Associated with Disease Severity and Activity by Radwa El- Sayed Mahmoud Marie, Noha M. Abd El-Fadeel, Yara El-Sayed Marei, Lina M. Atef

“…Methods. CD70 and CD27 mRNA expressions were evaluated by a quantitative real-time polymerase chain reaction in scalp biopsies from 40 AA patients (both AA lesions and non-lesional areas) and 40 healthy controls (HCs). …”
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The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat by Shaohui Zong, Gaofeng Zeng, Ye Fang, Jinzhen Peng, Yong Tao, Keke Li, Jingmin Zhao

“…RT-PCR was used to analyze the mRNA expression of IL-17 and IL-23p19 in the spleen tissue. …”
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Sphingosine-1-Phosphate/Sphingosine-1-Phosphate Receptor 2 Axis Can Promote Mouse and Human Primary Mast Cell Angiogenic Potential through Upregulation of Vascular Endothelial Grow... by Alena Chumanevich, Piper Wedman, Carole A. Oskeritzian

“…Further, we show that S1P stimulation triggered transcriptional upregulation of VEGF-A and MMP-2 mRNA in human but not in mouse MC. S1P exposure also triggered MMP-2 secretion from human MC. …”
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Characterizing the Crosstalk of NCAPG with Tumor Microenvironment and Tumor Stemness in Stomach Adenocarcinoma by Zheng Xiang, Genlan Cha, Yihao Wang, Jikai Gao, Jianguang Jia

“…In addition, we found a higher mRNA stemness index (mRNAsi) in the high-risk group and a positive correlation between NCAPG expression and mRNAsi. …”
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Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis by Dan Yu, Yong Chen, Ming Luo, Yanjin Peng, Shengen Yi

“…Compared with adjacent stomach tissues, gastric adenocarcinoma tissues showed significantly higher SLC39A1 on both mRNA and protein levels. Higher SLC39A1 was observed in patients with larger tumor size (P=0.003) and advanced tumor stages (P<0.001). …”
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Effects of central administration of the human Tau proteinon the <i>Bdnf, Trkb, p75, Mapt, Bax</i> and <i>Bcl-2</i> genes expression in the mouse brain by A. S. Oreshko, A. Ya. Rodnyy, D. V. Bazovkina, V. S. Naumenko

“…., a notable decrease in the mRNA level of the gene encoding the most important neurotrophic factor BDNF (brain-derived neurotrophic factor) was observed in the frontal cortex which could play an important role in neurodegeneration caused by pathological Tau protein aggregation. …”
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Effect of Toll-Like Receptor 4 on Synovial Injury of Temporomandibular Joint in Rats Caused by Occlusal Interference by Jingjing Kong, Yingying Yang, Shuzhen Sun, Jianli Xie, Xuefen Lin, Ping Ji

“…The results showed that obvious inflammation changes were observed in the synovial membranes and the expression of TLR4 and IL-1β was increased at both mRNA and protein levels in the occlusal interference rats. …”
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Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells by Qiong Wu, Jinxi Zhan, Yun Li, Xiaoxia Wang, Lu Xu, Juan Yu, Shiming Pu, Zuping Zhou

“…Integrated analysis of microRNA and mRNA expression indicated that 20 microRNAs and 49 mRNAs were inversely associated with Sca-1+CD31− and Sca-1+CD31+ subtype stemness characteristics. …”
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Anandamide, Acting via CB2 Receptors, Alleviates LPS-Induced Neuroinflammation in Rat Primary Microglial Cultures by Natalia Malek, Katarzyna Popiolek-Barczyk, Joanna Mika, Barbara Przewlocka, Katarzyna Starowicz

“…Microglial activation was assessed based on nitric oxide (NO) production. Analysis of mRNA was conducted for M1 and M2 phenotype markers possibly affected by the treatment. …”
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De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle by Teng Xu, Xudong Guo, Hui Wang, Xiaoyuan Du, Xiaoyu Gao, Dongjun Liu

“…In the present study, using Illumina/Solexa sequencing technology, we de novo assembled 130 million mRNA-Seq reads into a total of 49,115 contigs. Searching public databases revealed that about 45% of the total contigs can be annotated as known proteins, indicating that some of the assembled contigs may have previously uncharacterized functions. …”
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Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation by Nagarajan Selvamurugan, Zhiming He, Daniel Rifkin, Branka Dabovic, Nicola C. Partridge

“…PEMF stimulated activation of Smad2 and miR21-5p expression in differentiated osteoblasts, and TGF-β signaling was essential for PEMF stimulation of alkaline phosphatase mRNA expression. Smad7, an antagonist of the TGF-β signaling pathway, was found to be miR21-5p’s putative target gene and PEMF caused a decrease in Smad7 expression. …”
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Tuberculosis vaccines and therapeutic drug: challenges and future directions by Yajing An, Ruizi Ni, Li Zhuang, Ling Yang, Zhaoyang Ye, Linsheng Li, Seppo Parkkila, Ashok Aspatwar, Wenping Gong

“…We spotlight innovative approaches, such as multi-antigen vaccines and mRNA technology platforms. Furthermore, the review delves into current TB therapeutics, particularly for multidrug-resistant tuberculosis (MDR-TB), exploring promising agents like bedaquiline (BDQ) and delamanid (DLM), as well as the potential of host-directed therapies. …”
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Human Placenta-Derived Mesenchymal Stem Cells Reduce Mortality and Hematoma Size in a Rat Intracerebral Hemorrhage Model in an Acute Phase by Bo Young Choi, Ok Joon Kim, Sae-Hong Min, Jeong Hyun Jeong, Sang Won Suh, Tae Nyoung Chung

“…Expression of tight junction proteins (ZO-1, occludin) and coagulation factor VII mRNA was also compared. Mortality rate (50% versus 8.3%), hematoma size, ventricular size, hemispheric enlargement, and degenerating neuron count were significantly lower in the MSC-treated group (p=0.034, 0.038, 0.001, 0.022, and <0.001, resp.), while the expression of ZO-1 and occludin was higher (p=0.007 and 0.012). …”
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cAMP analogues downregulate the expression of granulocyte macrophage colony-stimulating factor (GM-CSF) in human bone marrow stromal cells in vitro by G. Bug, J. Aman, C. Huber, C. Peschel, H. G. Derigs

“…The coincubation with 8-bromo-cAMP (8BrcAMP), a water soluble cAMP analogue, inhibited this GM-CSF stimulation at the protein and the m RNA level. This effect was dose dependent with a maximal inhibition of about 65% occurring at a 8BrcAMP concentration of 0.75 mM. …”
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Modulation of Apoptosis and ER Stress Markers in Hepatocellular Carcinoma Cells by Irinotecan, Hesperidin, and Piperine by Serkan Sen, Kardelen Kocaman Kalkan, Canan Yilmaz, Sefa Celik

“…Researchers performed total RNA extraction and quantitative PCR to assess mRNA levels of Bad, Bax, and p53 (apoptosis-related genes) and ATF4, CHOP, and GRP78 (ER stress markers). …”
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Inhibition of Synovial Macrophage Pyroptosis Alleviates Synovitis and Fibrosis in Knee Osteoarthritis by Li Zhang, Runlin Xing, Zhengquan Huang, Nongshan Zhang, Li Zhang, Xiaochen Li, Peimin Wang

“…In the synovial tissue of KOA model rats, we observed a decrease of caspase1, NLRP3, ASC, and GSDMD caused by macrophage depletion in both the mRNA and protein expressions. Besides, rats treated with the specific caspase1 inhibitor Ac-YVAD-CMK showed less inflammatory reaction and fibrosis, not only in the expression of proinflammatory factors IL-1β, IL-18, and HMGB1 and fibrosis markers TGF-β, PLOD2, COL1A1, and TIMP1 but also in the observation of HE staining, Sirius Red staining, and the transverse diameters of the right knees. …”
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