An Integrated Database for Exploring Alternative Promoters in Animals by Feiyang Xue, Yuqin Yan, Weiwei Jin, Haotian Zhu, Yanbo Yang, Zhanhui Yu, Xuewen Xu, Jing Gong, Xiaohui Niu

“…Abstract Alternative promoter (AP) events, as a major pre-transcriptional mechanism, can initiate different transcription start sites to generate distinct mRNA isoforms and regulate their expression. At present, hundreds of thousands of APs have been identified across human tissues, and a considerable number of APs have been demonstrated to be associated with complex traits and diseases. …”
Get full text

Differential Expression of Matrix Metalloproteases in Human Fibroblasts with Different Origins by Diana Lindner, Christin Zietsch, P. Moritz Becher, Karsten Schulze, Heinz-Peter Schultheiss, Carsten Tschöpe, Dirk Westermann

“…Interestingly, we found major differences in basal mRNA expression especially for MMP1 and MMP3. Moreover, we treated fibroblasts with TNF-α and observed different alterations under these proinflammatory conditions. …”
Get full text

New insights into N6-methyladenosine in hepatocellular carcinoma immunotherapy by Mengran Li, Mengran Li, Hu Tian, Yanshuang Zhuang, Zili Zhang, Zili Zhang

“…This modification is crucial for controlling important functions that are vital for gene expression, including mRNA splicing, stability, and translation. Due to its intricate participation in both normal cellular processes and the course of disease, as well as its critical role in determining cell fate, N6-methyladenosine (m6A) alteration has recently attracted a lot of interest. …”
Get full text

The Protective Effect of Lidocaine on Septic Rats via the Inhibition of High Mobility Group Box 1 Expression and NF-κB Activation by Huan-Liang Wang, Yan-Qiu Xing, Ying-Xue Xu, Fei Rong, Wei-Fu Lei, Wen-Hua Zhang

“…The mechanism was relative to the inhibitory effect of lidocaine on the mRNA expression level of HMGB1 in multiple organs, release of HMGB1 to plasma, and activation of NF-κB.…”
Get full text

RETRACTED: Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (<em>Danio rerio</em>) by Davide Di Paola, Fabiano Capparucci, Sabrina Natale, Rosalia Crupi, Salvatore Cuzzocrea, Nunziacarla Spanò, Enrico Gugliandolo, Alessio Filippo Peritore

“…Morphological alteration, mucus production, messenger RNA (mRNA) expression related to intestinal function and oxidative stress were measured. …”
Get full text

An Interactive Database of Cocaine-Responsive Gene Expression by Willard M. Freeman, Kathryn E. Dougherty, Sally E. Vacca, Kent E. Vrana

“…The database is limited to statistically significant changes in mRNA or protein expression after cocaine administration. …”
Get full text

Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α by Ken Shirato, Kazuhiko Imaizumi

“…Although LPS-stimulated secretion of tumor necrosis factor-α (TNF-α) by the macrophages from middle-aged mice was significantly lower than that from young mice, LPS caused marked increases in levels of TNF-α mRNA in macrophages from middle-aged as well as young mice. …”
Get full text

Identification and Validation of a Potential Stemness-Associated Biomarker in Hepatocellular Carcinoma by Yangyang Zhang, Ruike Zhang, Lingxiu Zeng, Haizhou Wang, Ruyi Peng, Meng Zhang, Hailin Zhang, Zhenwei Yang, Liping Gao, Meng Wang, Jing Liu

“…The data of HCC patients from The Cancer Genome Atlas (TCGA) were collected and divided based on the mRNA expression-based stemness index (mRNAsi) in this study. …”
Get full text

XRCC5 VNTR, XRCC6 -61C>G, and XRCC7 6721G>T Gene Polymorphisms Associated with Male Infertility Risk: Evidences from Case-Control and In Silico Studies by Danial Jahantigh, Abasalt Hosseinzadeh Colagar

“…The XRCC7 6721G>T gene polymorphism occurred in an acceptor-splicing site, but this polymorphism has no severe modification on XRCC7 mRNA splicing. Our results indicate the association of XRCC5 VNTR, XRCC6 -61C>G, and XRCC7 6721G>T gene polymorphisms with male infertility in Iranian men.…”
Get full text

Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit by Xiao Qin, Peng Wang, Yongming Huang, Yansen Li, Min Chao, Wei Wang

“…High-throughput RNA sequencing was also performed to investigate the anal tissue (normal and ADSC treatment group) expression profile of mRNA. Our data showed that ADSC treatment had the shortest time to promote fistula healing compared to the other treatments, and fistula filled with new adipose tissue and muscle cells without scar. …”
Get full text

Modern vaccines and coronavirus infections by Yu. A. Belikova, Yu. V. Samsonov, E. V. Abakushina

“…This review is on recent advances in the development of new generation vaccines, as well as those developed using earlier time-tested technologies: live attenuated vaccines, inactivated vaccines, recombinant vaccines, subunit vaccines, virus-like particle-based vaccines, synthetic peptide vaccines, DNA vaccines and mRNA vaccines. However, many infections are still not preventable with the currently available vaccines and they represent a major cause of mortality worldwide — for example, severe acute respiratory syndrome coronavirus, middle east respiratory syndrome coronavirus and severe acute respiratory syndrome coronavirus 2 that causes coronavirus disease 2019 — COVID-19. …”
Get full text

Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier by Shinobu Watarai, Tana Iwase, Tomoko Tajima, Eiji Yuba, Kenji Kono

“…In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ-(Th1-type-) and IL-4-(Th2 type-) specific mRNA were detected. Moreover, substantial production of IFN-γ and IL-4 was demonstrated in spleen cells from OVA-containing SucPG-modified liposomes in vitro. …”
Get full text

Berberine decorated zinc oxide loaded chitosan nanoparticles a potent anti cancer agent against breast cancer by Fatemeh Esnaashari, Hojjatolah Zamani, Hossein Zahmatkesh, Mojtaba Soleimani, Golnesa Amirian Dashtaki, Behnam Rasti

“…Also, annexin V-FITC/PI double staining, Hoechst 33,342 staining, caspases-8 and 9 activity assays, and cell cycle analysis were performed. Furthermore, the mRNA levels of Bax and Bcl-2 genes were quantified using qPCR. …”
Get full text

Prognostic Value of GPNMB, EGFR, p-PI3K, and Ki-67 in Patients with Esophageal Squamous Cell Carcinoma by Bo Wang, Mengyan Li, Anna Su, Yongmei Gao, Yan Shi, Chao Li, Wenying Liu, Liping Su, Wan Li, Yuqing Ma

“…Statistical analysis was done by SPSS 22.0 and R. Results. GPNMB mRNA expression is higher in ESCC compared with paracancerous tissues. …”
Get full text

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines by Jodie R. Malcolm, Katherine S. Bridge, Andrew N. Holding, William J. Brackenbury

“…Reference genes (RGs) are endogenous RT-qPCR controls used to normalise mRNA levels, allowing accurate assessment of transcriptional changes. …”
Get full text

Local Gene Transfer of OPG Prevents Joint Damage and Disease Progression in Collagen-Induced Arthritis by Qingguo Zhang, Weiming Gong, Bin Ning, Lin Nie, Paul H. Wooley, Shang-You Yang

“…MicroCT data suggested significant protection against subchondral bone mineral density changes in OPG treated CIA mice. Interestingly, mRNA expressions of IFN-g and MMP3 were noticeably diminished following OPG gene transfer. …”
Get full text

Pan-Cancer Analyses of the Tumor Microenvironment Reveal That Ubiquitin-Conjugating Enzyme E2C Might Be a Potential Immunotherapy Target by Guang-zhao Huang, Ze-qun Chen, Juan Wu, Ting-ru Shao, Chen Zou, Yi-long Ai, Xiao-zhi Lv

“…Furthermore, the UBE2C mRNA level and protein level were all identified as upregulated in OSCC cell lines and tissues. …”
Get full text

The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats by Yusu Ni, Diyan Chen, Yi Jiang, Danhong Qiu, Wen Li, Huawei Li

“…Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. …”
Get full text

Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22T/VGH Cells Treated with Epirubicin by Wen-Tsan Chang, Hsiao-Ling Cheng, Bau-Shan Hsieh, Chien-Chih Chiu, King-Teh Lee, Kee-Lung Chang

“…The combination also caused a decrease in autophagy and in the expression of multidrug resistance-related protein 1 mRNA compared to epirubicin alone. This study shows the epirubicin/progesterone combination was more effective in increasing apoptosis and inversely decreasing autophagy on HA22T/VGH cells treated with epirubicin alone, suggesting that this combination can potentially be used to treat HCC.…”
Get full text

Computer analysis shows differences between mitochondrial miRNAs and other miRNAs by P. S. Vorozheykin, I. I. Titov

“…The analysis revealed, for the first time, a significant difference between mitomiRs and other microRNAs by the following criteria (in descending order of importance in the classification): mitomiRs are evolutionarily older (have a lower phylostratigraphic age index, PAI); have more targets and disease associations, including mitochondrial ones (twosided Fisher’s exact test, average p-values 1.82×10–89/1.13×10–96 for all mRNA/diseases and 6.01×10–22/1.09×10–9 for mitochondria, respectively); and are in the class of “circulating” miRNAs (average pvalue 1.20×10–56). …”
Get full text